MD16G1062100.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016506
Physical Location & Seq
Reverse (-)
4419295 .. 4422061
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1062100.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCGTCGCCCAAGAAGGAGAGAGAGACCCAGGTTTACTTGGCCAAGCTCGCTGAGCAGGCCGAGCGATACGAAGAAATGGTTGAGTGTATGAAGAAAGTTGCGAAACTTGACCTTGAGCTGACTGTAGAGGAGAGGAATCTTCTTTCTGTGGGATACAAAAATGTCATAGGTGCTCGAAGGGCCTCTTGGCGCATCATGTCTTCCATTGAGCAAAAGGAAGAGTCTAAGGGAAATGAGCACAATGTCAAACTGATCAAGGGTTACCGGGTGAAGGTGGAGGAGGAACTCTCCAAGATTTGCGATGACATTCTGTCAATCATAGACAAGCACCTGATCCCCTCTTCCTCCTCTGGAGAGGCCACTGTTTTCTACTACAAGATGAAAGGTGACTATTATCGGTACCTTGCTGAGTTCAAGACCGATCAGGACAGAAAAGAGGCAGCTGAGCAGTCACTCAAGGGATACGAGGCATGTTTAATTTTACCAATTTTAACACAAATGGATGCTGCATCAGCCACTGCAAACAAAGATCTCCCTTCAACCCACCCAATCCGTCTTGGTCTAGCCCTCAACTTCTCTGTCTTCTACTATGAGATTATGAATTCTCCCGAGAGGGCTTGCCATTTGGCTAAACAGGCATTTGATGAGGCAATTGCAGAGTTGGACACCTTGAGTGAGGAGTCATACAAGGACAGCACCCTTATCATGCAATTGTTGAGAGACAACCTCACTCTCTGGACCTCTGATTTGCCTGAAGACGGAGGTGATGACAACTTCAAAGGTGAAGAATCCAAGCCTGCCGCCGAACCCGCAACTGCTAAGCATTCACGGGCTTACCTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

283

Amino Acids

32.03

Weight (kDa)

5.22

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 248 6.1e-102 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26480
fragaria_vesca FvH4_4g30210
malus_domestica MD13G1063600.v1.1 MD16G1062100.v1.1
prunus_persica Prupe.1G285800_v2.0.a1
pyrus_communis pycom13g05620
rosa_chinensis RchiOBHm_Chr4g0438531
rosa_laevigata RLG00000006354
rosa_multiflora Rmu_sc0002327.1_g000007
rosa_roxburghii Rroxscaffold_5G00379530
rosa_rugosa Rorug04G0311100
rosa_samantha Rh4DG367600
rosa_wichuraiana Rw0G006620 Rw0G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 402
AciI CCGC 2 cut(s) 804, 813
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 777
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 2 cut(s) 274, 761
AgsI TTSAA 3 cut(s) 418, 543, 781
AhdI GACNNNNNGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 172, 204
AluBI AGCT 3 cut(s) 49, 121, 446
AluI AGCT 3 cut(s) 49, 121, 446
Alw21I GWGCWC 2 cut(s) 178, 243
Alw26I GTCTC 2 cut(s) 20, 717
AlwI GGATC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 4 cut(s) 42, 60, 183, 360
ApeKI GCWGC 2 cut(s) 443, 509
ApoI RAATTY 1 cut(s) 604
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 183, 741
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 4 cut(s) 283, 401, 779, 797
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 1 cut(s) 741
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 402
BbsI GAAGAC 3 cut(s) 195, 577, 765
Bbv12I GWGCWC 2 cut(s) 178, 243
BbvI GCAGC 2 cut(s) 455, 496
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 2 cut(s) 149, 458
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 20, 717
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 126
BfuI GTATCC 2 cut(s) 149, 458
BglII AGATCT 1 cut(s) 532
BisI GCNGC 3 cut(s) 444, 510, 804
BlpI GCTNAGC 3 cut(s) 54, 447, 822
BlsI GCNGC 3 cut(s) 445, 511, 805
Bme1390I CCNGG 2 cut(s) 32, 269
Bme18I GGWCC 1 cut(s) 741
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 2 cut(s) 183, 741
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 32, 269
BmsI GCATC 3 cut(s) 204, 496, 521
BpiI GAAGAC 3 cut(s) 195, 577, 765
BplI GAGNNNNNCTC 4 cut(s) 275, 307, 714, 746
BpmI CTGGAG 1 cut(s) 375
Bpu1102I GCTNAGC 3 cut(s) 54, 447, 822
BpuEI CTTGAG 3 cut(s) 137, 443, 694
BpuMI CCSGG 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 761
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 274, 761
BseMII CTCAG 3 cut(s) 45, 402, 438
BseRI GAGGAG 4 cut(s) 146, 296, 340, 695
BseXI GCAGC 2 cut(s) 455, 496
BshFI GGCC 4 cut(s) 44, 62, 185, 362
BshNI GGYRCC 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 178, 243
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 274, 761
BsmAI GTCTC 2 cut(s) 20, 717
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 4 cut(s) 44, 62, 185, 362
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 178, 243
Bsp143I GATC 4 cut(s) 255, 336, 424, 532
Bsp1720I GCTNAGC 3 cut(s) 54, 447, 822
BspACI CCGC 2 cut(s) 804, 813
BspANI GGCC 4 cut(s) 44, 62, 185, 362
BspCNI CTCAG 3 cut(s) 46, 403, 439
BspLI GGNNCC 1 cut(s) 404
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 402
BspTNI GGTCTC 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 255, 336, 424, 532
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 127, 367
Bst6I CTCTTC 2 cut(s) 216, 349
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 51, 60, 622, 801
BstDEI CTNAG 5 cut(s) 54, 228, 411, 447, 822
BstEII GGTNACC 1 cut(s) 263
BstF5I GGATG 1 cut(s) 511
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 4 cut(s) 258, 339, 427, 535
BstMAI GTCTC 2 cut(s) 20, 717
BstMBI GATC 4 cut(s) 255, 336, 424, 532
BstMWI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 515, 638, 812
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 477
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 30, 267
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 455, 496
BstV2I GAAGAC 3 cut(s) 195, 577, 765
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuI GTATCC 2 cut(s) 149, 458
BsuRI GGCC 4 cut(s) 44, 62, 185, 362
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 363, 519
Cac8I GCNNGC 4 cut(s) 51, 60, 622, 801
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 2 cut(s) 183, 741
Csp6I GTAC 1 cut(s) 403
CviAII CATG 3 cut(s) 199, 474, 709
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 5 cut(s) 54, 228, 411, 447, 822
DpnI GATC 4 cut(s) 257, 338, 426, 534
DpnII GATC 4 cut(s) 255, 336, 424, 532
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 216, 349
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 2 cut(s) 216, 349
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 741
Eco57I CTGAAG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 611
Eco91I GGTNACC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 604
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 3 cut(s) 202, 477, 712
FaiI YATR 9 cut(s) 92, 170, 200, 323, 475, 594, 602, 688, 710
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 3 cut(s) 198, 473, 708
FauI CCCGC 1 cut(s) 820
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 444, 510, 804
FokI GGATG 1 cut(s) 518
Fsp4HI GCNGC 3 cut(s) 444, 510, 804
FspBI CTAG 1 cut(s) 566
GlaI GCGC 1 cut(s) 194
GluI GCNGC 3 cut(s) 444, 510, 804
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 4 cut(s) 44, 62, 185, 362
HapII CCGG 1 cut(s) 268
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 202, 477, 712
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 4 cut(s) 139, 224, 683, 791
HpaII CCGG 1 cut(s) 268
HphI GGTGA 4 cut(s) 283, 401, 779, 797
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 748
Hpy188III TCNNGA 5 cut(s) 354, 418, 428, 611, 739
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 10, 174, 268, 549
HpyCH4III ACNGT 2 cut(s) 127, 367
HpyCH4V TGCA 4 cut(s) 512, 524, 659, 712
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 515, 638, 812
HpyF3I CTNAG 5 cut(s) 54, 228, 411, 447, 822
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 202, 477, 712
HspAI GCGC 1 cut(s) 193
KpnI GGTACC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 4 cut(s) 255, 336, 424, 532
Lsp1109I GCAGC 2 cut(s) 455, 496
LweI GCATC 3 cut(s) 204, 496, 521
MaeI CTAG 1 cut(s) 566
MaeIII GTNAC 3 cut(s) 263, 389, 453
MalI GATC 4 cut(s) 257, 338, 426, 534
MboI GATC 4 cut(s) 255, 336, 424, 532
MboII GAAGA 9 cut(s) 86, 106, 134, 195, 233, 336, 577, 770, 800
MfeI CAATTG 2 cut(s) 654, 713
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 178, 243
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 5 cut(s) 480, 489, 604, 654, 713
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 233, 692
MmeI TCCRAC 1 cut(s) 645
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 479, 494
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 32, 269
MunI CAATTG 2 cut(s) 654, 713
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 515, 638, 812
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 255, 336, 424, 532
NlaIII CATG 3 cut(s) 202, 477, 712
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 389, 453
NspI RCATGY 1 cut(s) 477
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 2 cut(s) 139, 791
PkrI GCNGC 3 cut(s) 445, 511, 805
PleI GAGTC 2 cut(s) 232, 691
PpsI GAGTC 2 cut(s) 232, 691
Psp6I CCWGG 1 cut(s) 30
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 2 cut(s) 183, 741
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 479, 494
SatI GCNGC 3 cut(s) 444, 510, 804
Sau3AI GATC 4 cut(s) 255, 336, 424, 532
Sau96I GGNCC 2 cut(s) 183, 741
SchI GAGTC 2 cut(s) 233, 692
ScrFI CCNGG 2 cut(s) 32, 269
SduI GDGCHC 2 cut(s) 178, 243
SfaNI GCATC 3 cut(s) 204, 496, 521
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 741
SmlI CTYRAG 3 cut(s) 116, 458, 673
SmoI CTYRAG 3 cut(s) 116, 458, 673
Sse9I AATT 5 cut(s) 480, 489, 604, 654, 713
SsiI CCGC 2 cut(s) 804, 813
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 30, 267
TaaI ACNGT 2 cut(s) 127, 367
TaqI TCGA 1 cut(s) 178
TaqII GACCGA 1 cut(s) 437
TasI AATT 5 cut(s) 480, 489, 604, 654, 713
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 2 cut(s) 139, 791
Tru1I TTAA 2 cut(s) 479, 494
Tru9I TTAA 2 cut(s) 479, 494
TscAI CASTG 2 cut(s) 370, 526
TseFI GTSAC 2 cut(s) 389, 453
TseI GCWGC 2 cut(s) 443, 509
Tsp45I GTSAC 2 cut(s) 389, 453
TspDTI ATGAA 3 cut(s) 107, 398, 617
TspGWI ACGGA 2 cut(s) 545, 777
TspRI CASTG 2 cut(s) 370, 526
VpaK11BI GGWCC 1 cut(s) 741
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 477
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.