MD13G1063600.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016506
Physical Location & Seq
Reverse (-)
4396805 .. 4399529
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1063600.v1.1.491

Sequence Viewer

Length: 792 bp
ATGTCGTCGCCCGAGAAGGAGAGAGAGTCCCAGGTTTACATGGCCAAGCTCGCCGAGCAGGCCGAGCGTTACGAAGAAATGGTTGAGTGTATGAAGAAAGTTGCGAAACTTGACCTTGAGCTGACTGTAGAGGAGAGGAATCTTCTTTCTGTGGGATACAAAAATGTCATAGGTGCTCGACGGGCCTCTTGGCGCATCATGTCTTCCATTGAGCAAAAGGAAGAGTCTAAGGGGAATGAGCACAATGTGAAACTGATCAAGGGTTACCGGGTGAAGGTGGAGGAGGAACTCTCCAAGATTTGCAATGACATTCTGTCAATCATAGACAAGCACCTGATTCCCTCTTCCAACTCTGGAGAGGCCACTGTTTTCTACTACAAGATGAAAGGTGACTATTACCGGTACCTTTCTGAGTTCAAGACCGATCAGGACAAAAAAGAGGCAGCTGATCAGTCACTCAAGGGATACGAGGCTGCATCAGCCACTGCAAACACAGATCTCCCATCAACCCACCCAATCCATCTTGGTCTAGCCCTCAACTTCTCTGTCTTCTACTATGAGATTATGAATTCTCCTGAGAGGGCTTGCCATTTGGCTAAACAAGCATTTGATGAGGCAATTGCAGAGTTGGACACCTTGAGTGAGGAGTCATACAAGGACAGCACCCTTATCATGCAATTGTTGAGAGACAACCTCACTCTCTGGACCTCTGATTTGCCTGAAGACGGAGGTGATGACAACTTCAAAGGTGAAGAATCCAAACATGCCGCCGAATCTGCAACTGATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

264

Amino Acids

29.91

Weight (kDa)

4.96

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 236 1.4e-107 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26480
fragaria_vesca FvH4_4g30210
malus_domestica MD13G1063600.v1.1 MD16G1062100.v1.1
prunus_persica Prupe.1G285800_v2.0.a1
pyrus_communis pycom13g05620
rosa_chinensis RchiOBHm_Chr4g0438531
rosa_laevigata RLG00000006354
rosa_multiflora Rmu_sc0002327.1_g000007
rosa_roxburghii Rroxscaffold_5G00379530
rosa_rugosa Rorug04G0311100
rosa_samantha Rh4DG367600
rosa_wichuraiana Rw0G006620 Rw0G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 402
AciI CCGC 1 cut(s) 768
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 1 cut(s) 741
AdeI CACNNNGTG 1 cut(s) 247
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 2 cut(s) 274, 725
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 2 cut(s) 418, 745
AhdI GACNNNNNGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 49, 121, 446
AluI AGCT 3 cut(s) 49, 121, 446
Alw21I GWGCWC 2 cut(s) 178, 243
Alw26I GTCTC 1 cut(s) 681
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 4 cut(s) 42, 60, 183, 360
ApeKI GCWGC 2 cut(s) 443, 473
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 2 cut(s) 697, 729
AsiGI ACCGGT 1 cut(s) 399
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 183, 705
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 4 cut(s) 283, 401, 743, 761
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 705
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 402
BbsI GAAGAC 3 cut(s) 195, 541, 729
Bbv12I GWGCWC 2 cut(s) 178, 243
BbvI GCAGC 2 cut(s) 455, 460
BccI CCATC 2 cut(s) 511, 528
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 2 cut(s) 149, 458
BclI TGATCA 2 cut(s) 255, 448
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 126
BfuI GTATCC 2 cut(s) 149, 458
BglI GCCNNNNNGGC 1 cut(s) 59
BglII AGATCT 1 cut(s) 496
BisI GCNGC 3 cut(s) 444, 474, 768
BlsI GCNGC 3 cut(s) 445, 475, 769
Bme1390I CCNGG 2 cut(s) 32, 269
Bme18I GGWCC 1 cut(s) 705
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 183, 705
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 32, 269
BmsI GCATC 2 cut(s) 204, 485
BpiI GAAGAC 3 cut(s) 195, 541, 729
BplI GAGNNNNNCTC 4 cut(s) 275, 307, 678, 710
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 3 cut(s) 137, 443, 658
BpuMI CCSGG 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 725
Bse118I RCCGGY 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 274, 725
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 402, 567
BseRI GAGGAG 3 cut(s) 146, 296, 659
BseXI GCAGC 2 cut(s) 455, 460
BshFI GGCC 4 cut(s) 44, 62, 185, 362
BshNI GGYRCC 1 cut(s) 402
BshTI ACCGGT 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 178, 243
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 2 cut(s) 268, 400
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 274, 725
BsmAI GTCTC 1 cut(s) 681
BsmFI GGGAC 1 cut(s) 13
BsnI GGCC 4 cut(s) 44, 62, 185, 362
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 178, 243
Bsp143I GATC 4 cut(s) 255, 424, 448, 496
BspACI CCGC 1 cut(s) 768
BspANI GGCC 4 cut(s) 44, 62, 185, 362
BspCNI CTCAG 2 cut(s) 403, 568
BspLI GGNNCC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 255, 424, 448, 496
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 127, 367
Bst6I CTCTTC 2 cut(s) 216, 349
BstC8I GCNNGC 3 cut(s) 51, 60, 586
BstDEI CTNAG 3 cut(s) 228, 411, 576
BstEII GGTNACC 1 cut(s) 263
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 4 cut(s) 258, 427, 451, 499
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 4 cut(s) 255, 424, 448, 496
BstMWI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 479, 602, 776
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 767
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 30, 267
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 455, 460
BstV2I GAAGAC 3 cut(s) 195, 541, 729
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 2 cut(s) 149, 458
BsuRI GGCC 4 cut(s) 44, 62, 185, 362
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 363, 483
Cac8I GCNNGC 3 cut(s) 51, 60, 586
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 183, 705
Csp6I GTAC 1 cut(s) 403
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 4 cut(s) 40, 199, 673, 764
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 228, 411, 576
DpnI GATC 4 cut(s) 257, 426, 450, 498
DpnII GATC 4 cut(s) 255, 424, 448, 496
DraIII CACNNNGTG 1 cut(s) 247
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 216, 349
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 2 cut(s) 216, 349
Eco47I GGWCC 1 cut(s) 705
Eco57I CTGAAG 1 cut(s) 741
Eco88I CYCGRG 1 cut(s) 11
Eco91I GGTNACC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 43, 202, 676, 767
FaqI GGGAC 1 cut(s) 13
FatI CATG 4 cut(s) 39, 198, 672, 763
FbaI TGATCA 2 cut(s) 255, 448
Fnu4HI GCNGC 3 cut(s) 444, 474, 768
Fsp4HI GCNGC 3 cut(s) 444, 474, 768
FspBI CTAG 1 cut(s) 530
GlaI GCGC 1 cut(s) 194
GluI GCNGC 3 cut(s) 444, 474, 768
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 4 cut(s) 44, 62, 185, 362
HapII CCGG 2 cut(s) 268, 400
HhaI GCGC 1 cut(s) 195
Hin1II CATG 4 cut(s) 43, 202, 676, 767
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 7 cut(s) 26, 139, 224, 337, 647, 755, 773
HpaII CCGG 2 cut(s) 268, 400
HphI GGTGA 4 cut(s) 283, 401, 743, 761
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 2 cut(s) 412, 712
Hpy188III TCNNGA 5 cut(s) 354, 418, 428, 575, 703
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 2 cut(s) 10, 183
HpyAV CCTTC 2 cut(s) 10, 268
HpyCH4III ACNGT 2 cut(s) 127, 367
HpyCH4V TGCA 6 cut(s) 303, 476, 488, 623, 676, 779
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 479, 602, 776
HpyF3I CTNAG 3 cut(s) 228, 411, 576
Hsp92II CATG 4 cut(s) 43, 202, 676, 767
HspAI GCGC 1 cut(s) 193
KpnI GGTACC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 255, 448
Kzo9I GATC 4 cut(s) 255, 424, 448, 496
Lsp1109I GCAGC 2 cut(s) 455, 460
LweI GCATC 2 cut(s) 204, 485
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 4 cut(s) 68, 263, 389, 453
MalI GATC 4 cut(s) 257, 426, 450, 498
MboI GATC 4 cut(s) 255, 424, 448, 496
MboII GAAGA 9 cut(s) 86, 106, 134, 195, 233, 336, 541, 734, 764
MfeI CAATTG 2 cut(s) 618, 677
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 178, 243
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 3 cut(s) 568, 618, 677
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 3 cut(s) 35, 233, 656
MmeI TCCRAC 2 cut(s) 372, 609
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 268, 400
MspR9I CCNGG 2 cut(s) 32, 269
MunI CAATTG 2 cut(s) 618, 677
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 8 cut(s) 50, 55, 59, 64, 182, 479, 602, 776
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 255, 424, 448, 496
NlaIII CATG 4 cut(s) 43, 202, 676, 767
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 2 cut(s) 79, 88
NmuCI GTSAC 2 cut(s) 389, 453
NspI RCATGY 1 cut(s) 767
PfeI GAWTC 4 cut(s) 139, 337, 755, 773
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 3 cut(s) 445, 475, 769
PleI GAGTC 3 cut(s) 34, 232, 655
PpsI GAGTC 3 cut(s) 34, 232, 655
Psp6I CCWGG 1 cut(s) 30
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 2 cut(s) 183, 705
PstNI CAGNNNCTG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SatI GCNGC 3 cut(s) 444, 474, 768
Sau3AI GATC 4 cut(s) 255, 424, 448, 496
Sau96I GGNCC 2 cut(s) 183, 705
SchI GAGTC 3 cut(s) 35, 233, 656
ScrFI CCNGG 2 cut(s) 32, 269
SduI GDGCHC 2 cut(s) 178, 243
SfaNI GCATC 2 cut(s) 204, 485
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 705
SmlI CTYRAG 3 cut(s) 116, 458, 637
SmoI CTYRAG 3 cut(s) 116, 458, 637
Sse9I AATT 3 cut(s) 568, 618, 677
SsiI CCGC 1 cut(s) 768
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 30, 267
TaaI ACNGT 2 cut(s) 127, 367
TaqI TCGA 1 cut(s) 178
TaqII GACCGA 1 cut(s) 437
TasI AATT 3 cut(s) 568, 618, 677
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 4 cut(s) 139, 337, 755, 773
TscAI CASTG 2 cut(s) 370, 490
TseFI GTSAC 2 cut(s) 389, 453
TseI GCWGC 2 cut(s) 443, 473
Tsp45I GTSAC 2 cut(s) 389, 453
TspDTI ATGAA 3 cut(s) 107, 398, 581
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 2 cut(s) 370, 490
VpaK11BI GGWCC 1 cut(s) 705
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 767
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.