Rw0G016570

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0016506
Physical Location & Seq
Reverse (-)
382983 .. 385386
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016570.1

Sequence Viewer

Length: 792 bp
ATGTCGTCGCCCGAGAAGGAGAGAGAGACCCAGGTTTACATGGCCAAGCTCGCCGAGCAGGCCGAGCGTTACGAAGAAATGGTCGAGTGTATGAAGAAAGTTGCGAAGCTTGATCTTGAGCTGACTGTGGAAGAGAGGAATCTTTTGTCTGTGGGATACAAAAATGTGATAGGTGCTCGAAGGGCGTCTTGGCGCATCATGTCTTCCATTGAGCAAAAGGAAGAGTCTAAAGGGAATGAGCACAATGTCAAACTGATCAAGAGTTACCGCCAGAAGGTGGAGGAGGAACTCTCCAAGATATGCAGTGACATTCTCACTATCATTGACAAGCACCTGATTCCCTCTTCCAGCTCCGGAGAGGCCACTGTTTTCTACTACAAGATGAAAGGTGACTATTACCGGTACCTTGCTGAGTTCAAGACTGACCAGGACAGAAAAGAGGCAGCTGACCAGTCACTCAAGGGATATGAGGCTGCTTCAGCCACTGCAAACACAGATCTCCCATCGACCCACCCAATCCGTCTTGGTCTAGCTCTCAACTTCTCTGTCTTCTACTATGAGATTATGAATTCTCCCGAGAGGGCTTGTCATTTGGCTAAACAAGCTTTTGATGAGGCAATTGCAGAGTTGGACACCCTGAGTGAGGAGTCATACAAGGACAGCACCCTTATCATGCAACTGTTGAGAGACAACCTCACTCTCTGGACCTCTGATTTGCCTGAAGATGGAGGTGATGACAATTTTAAAGCCGACGAAACCAAATCTATCGAACCTAATGCTGCTGCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

263

Amino Acids

29.93

Weight (kDa)

4.9

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 236 1.3e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26480
fragaria_vesca FvH4_4g30210
malus_domestica MD13G1063600.v1.1 MD16G1062100.v1.1
prunus_persica Prupe.1G285800_v2.0.a1
pyrus_communis pycom13g05620
rosa_chinensis RchiOBHm_Chr4g0438531
rosa_laevigata RLG00000006354
rosa_multiflora Rmu_sc0002327.1_g000007
rosa_roxburghii Rroxscaffold_5G00379530
rosa_rugosa Rorug04G0311100
rosa_samantha Rh4DG367600
rosa_wichuraiana Rw0G006620 Rw0G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 353
AciI CCGC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 2 cut(s) 462, 741
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 4 cut(s) 274, 277, 643, 725
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 2 cut(s) 30, 426
AjuI GAANNNNNNNTTGG 2 cut(s) 172, 204
AluBI AGCT 7 cut(s) 49, 109, 121, 351, 446, 533, 605
AluI AGCT 7 cut(s) 49, 109, 121, 351, 446, 533, 605
Alw21I GWGCWC 2 cut(s) 178, 243
Alw26I GTCTC 2 cut(s) 20, 681
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 2 cut(s) 11, 575
Aor13HI TCCGGA 1 cut(s) 353
AoxI GGCC 3 cut(s) 42, 60, 360
ApeKI GCWGC 4 cut(s) 443, 473, 779, 782
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 4 cut(s) 66, 98, 697, 729
AsiGI ACCGGT 1 cut(s) 399
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 705
AsuHPI GGTGA 2 cut(s) 401, 743
AvaI CYCGRG 2 cut(s) 11, 575
AvaII GGWCC 1 cut(s) 705
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 402
BbsI GAAGAC 2 cut(s) 195, 541
Bbv12I GWGCWC 2 cut(s) 178, 243
BbvI GCAGC 4 cut(s) 455, 460, 766, 769
BccI CCATC 2 cut(s) 511, 719
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 2 cut(s) 32, 428
BciVI GTATCC 1 cut(s) 149
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 20, 681
BfaI CTAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 59
BglII AGATCT 1 cut(s) 496
BisI GCNGC 4 cut(s) 444, 474, 780, 783
BlsI GCNGC 4 cut(s) 445, 475, 781, 784
Bme1390I CCNGG 2 cut(s) 32, 428
Bme18I GGWCC 1 cut(s) 705
BmeT110I CYCGRG 2 cut(s) 11, 575
BmgT120I GGNCC 1 cut(s) 705
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 32, 428
BmsI GCATC 1 cut(s) 204
BpiI GAAGAC 2 cut(s) 195, 541
BplI GAGNNNNNCTC 4 cut(s) 275, 307, 678, 710
BpuEI CTTGAG 2 cut(s) 137, 443
BsaHI GRCGYC 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 2 cut(s) 353, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 274, 277, 643, 725
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 451
BseAI TCCGGA 1 cut(s) 353
BseBI CCWGG 2 cut(s) 32, 428
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 4 cut(s) 274, 277, 643, 725
BseMII CTCAG 2 cut(s) 402, 629
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 296, 659
BseXI GCAGC 4 cut(s) 455, 460, 766, 769
BshFI GGCC 3 cut(s) 44, 62, 362
BshNI GGYRCC 1 cut(s) 402
BshTI ACCGGT 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 178, 243
BsiHKCI CYCGRG 2 cut(s) 11, 575
BsiSI CCGG 2 cut(s) 354, 400
BslI CCNNNNNNNGG 4 cut(s) 274, 277, 643, 725
BsmAI GTCTC 2 cut(s) 20, 681
BsnI GGCC 3 cut(s) 44, 62, 362
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 2 cut(s) 11, 575
Bsp1286I GDGCHC 2 cut(s) 178, 243
Bsp13I TCCGGA 1 cut(s) 353
Bsp143I GATC 3 cut(s) 112, 255, 496
BspACI CCGC 1 cut(s) 268
BspANI GGCC 3 cut(s) 44, 62, 362
BspCNI CTCAG 2 cut(s) 403, 630
BspEI TCCGGA 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 402
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 112, 255, 496
BssNI GRCGYC 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 32, 428
Bst4CI ACNGT 3 cut(s) 127, 367, 681
Bst6I CTCTTC 3 cut(s) 126, 216, 349
BstACI GRCGYC 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 51, 60
BstDEI CTNAG 2 cut(s) 411, 638
BstENI CCTNNNNNAGG 1 cut(s) 641
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 3 cut(s) 115, 258, 499
BstMAI GTCTC 2 cut(s) 20, 681
BstMBI GATC 3 cut(s) 112, 255, 496
BstMWI GCNNNNNNNGC 7 cut(s) 50, 55, 59, 64, 182, 479, 602
BstNI CCWGG 2 cut(s) 32, 428
BstSCI CCNGG 2 cut(s) 30, 426
BstV1I GCAGC 4 cut(s) 455, 460, 766, 769
BstV2I GAAGAC 2 cut(s) 195, 541
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 3 cut(s) 44, 62, 362
BtsI GCAGTG 2 cut(s) 310, 483
BtsIMutI CAGTG 3 cut(s) 310, 363, 483
Cac8I GCNNGC 2 cut(s) 51, 60
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 705
CseI GACGC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 403
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 3 cut(s) 40, 199, 673
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 411, 638
DpnI GATC 3 cut(s) 114, 257, 498
DpnII GATC 3 cut(s) 112, 255, 496
DraI TTTAAA 1 cut(s) 745
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 3 cut(s) 126, 216, 349
EarI CTCTTC 3 cut(s) 126, 216, 349
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 705
Eco57I CTGAAG 2 cut(s) 462, 741
Eco88I CYCGRG 2 cut(s) 11, 575
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 30, 426
FaeI CATG 3 cut(s) 43, 202, 676
FaiI YATR 9 cut(s) 41, 92, 200, 301, 468, 558, 566, 652, 674
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 3 cut(s) 39, 198, 672
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 4 cut(s) 444, 474, 780, 783
Fsp4HI GCNGC 4 cut(s) 444, 474, 780, 783
FspBI CTAG 1 cut(s) 530
GlaI GCGC 1 cut(s) 194
GluI GCNGC 4 cut(s) 444, 474, 780, 783
HaeIII GGCC 3 cut(s) 44, 62, 362
HapII CCGG 2 cut(s) 354, 400
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 3 cut(s) 43, 202, 676
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 2 cut(s) 107, 603
HinfI GANTC 4 cut(s) 139, 224, 337, 647
HpaII CCGG 2 cut(s) 354, 400
HphI GGTGA 2 cut(s) 401, 743
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 712
Hpy188III TCNNGA 6 cut(s) 116, 259, 354, 418, 575, 703
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 2 cut(s) 10, 755
HpyAV CCTTC 3 cut(s) 10, 174, 268
HpyCH4III ACNGT 3 cut(s) 127, 367, 681
HpyCH4V TGCA 4 cut(s) 303, 488, 623, 676
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 55, 59, 64, 182, 479, 602
HpyF3I CTNAG 2 cut(s) 411, 638
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 3 cut(s) 43, 202, 676
HspAI GCGC 1 cut(s) 193
Kpn2I TCCGGA 1 cut(s) 353
KpnI GGTACC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 3 cut(s) 112, 255, 496
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 4 cut(s) 455, 460, 766, 769
LweI GCATC 1 cut(s) 204
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 5 cut(s) 68, 263, 305, 389, 453
MalI GATC 3 cut(s) 114, 257, 498
MboI GATC 3 cut(s) 112, 255, 496
MboII GAAGA 8 cut(s) 86, 106, 143, 195, 233, 336, 541, 734
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 178, 243
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 3 cut(s) 568, 618, 739
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 233, 656
MmeI TCCRAC 1 cut(s) 609
Mox20I TGGCCA 1 cut(s) 44
MroI TCCGGA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 44
MseI TTAA 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 354, 400
MspR9I CCNGG 2 cut(s) 32, 428
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 32, 428
MwoI GCNNNNNNNGC 7 cut(s) 50, 55, 59, 64, 182, 479, 602
NdeII GATC 3 cut(s) 112, 255, 496
NlaIII CATG 3 cut(s) 43, 202, 676
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 2 cut(s) 79, 88
NmuCI GTSAC 3 cut(s) 305, 389, 453
PfeI GAWTC 2 cut(s) 139, 337
PflMI CCANNNNNTGG 1 cut(s) 277
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 4 cut(s) 445, 475, 781, 784
PleI GAGTC 2 cut(s) 232, 655
PpsI GAGTC 2 cut(s) 232, 655
Psp6I CCWGG 2 cut(s) 30, 426
PspGI CCWGG 2 cut(s) 30, 426
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 705
PstNI CAGNNNCTG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 744
SatI GCNGC 4 cut(s) 444, 474, 780, 783
Sau3AI GATC 3 cut(s) 112, 255, 496
Sau96I GGNCC 1 cut(s) 705
SchI GAGTC 2 cut(s) 233, 656
ScrFI CCNGG 2 cut(s) 32, 428
SduI GDGCHC 2 cut(s) 178, 243
SfaNI GCATC 1 cut(s) 204
SinI GGWCC 1 cut(s) 705
SmlI CTYRAG 2 cut(s) 116, 458
SmoI CTYRAG 2 cut(s) 116, 458
Sse9I AATT 3 cut(s) 568, 618, 739
SsiI CCGC 1 cut(s) 268
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 30, 426
TaaI ACNGT 3 cut(s) 127, 367, 681
TaqI TCGA 4 cut(s) 84, 178, 506, 768
TasI AATT 3 cut(s) 568, 618, 739
TfiI GAWTC 2 cut(s) 139, 337
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TscAI CASTG 3 cut(s) 310, 370, 490
TseFI GTSAC 3 cut(s) 305, 389, 453
TseI GCWGC 4 cut(s) 443, 473, 779, 782
Tsp45I GTSAC 3 cut(s) 305, 389, 453
TspDTI ATGAA 3 cut(s) 107, 398, 581
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 3 cut(s) 310, 370, 490
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 705
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 1 cut(s) 568
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.