FvH4_4g30590

Cell division topological specificity factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010355
Physical Location & Seq
Forward (+)
30125554 .. 30127885
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30590.t1

Sequence Viewer

Length: 675 bp
ATGGCTGGTATTGGGGATCTGAGAGTCTCGGCAACACTGGCTTCTCGTACGACGCACACTCTCCGGAGCTCTTTGCCGTCTTCGAAGGTAGAATCCGTCAGCTTTCTGAGCGGAGGGTCTAACATTTCTGAAATTAAACCAAAGTGGCCCGGGATAGCATTTGTCAGACGTGATATTCGTCAACATTCCAAGCGTTCTCTTGGAGATTTTCAAATGTCACCGAACTCCATTAACCAAGATGCTGAGAGCTTCCTCATTAACGCCATCAATATGAGTTTCTTTGAACGATTCAACTTGGCTTGGAAGATATTGTTCCCATCACCAGCCTCTAGAAGGAGCTCTAATGCAAATATAGCCAAGCAGCGCTTGAAGATGATCCTCTTCTCTGACCGATGTGCAGTTAGTGATGAGGCTAAACGGAAGATTGTCAACAACATCGTGCATGCTCTGTCTGATTTTGTGGAGATTGAATCACGCGACAAAGTTCAGCTGAGTGTGTCTACTGATACTGACCTCGGCACCATTTACTCAGTCACAGTGCCTGTAAGACGGGTTAAGCCAGAATATCAAATTGGAGATGAGACTGGAACAATAACAAATATCGAATACAAAGATACCGGAGAAAAGTCTGGTGCTGTAGATGTCAGGTTCGATTTCTTCGTCCCTGACGAATGA

Protein Analysis

225

Amino Acids

24.99

Weight (kDa)

9.01

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 116 - 183 1e-13 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 500
AccII CGCG 1 cut(s) 477
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 1 cut(s) 111
AclWI GGATC 2 cut(s) 24, 370
AfaI GTAC 1 cut(s) 49
AfeI AGCGCT 1 cut(s) 365
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 5 cut(s) 212, 284, 292, 370, 470
AjiI CACGTC 1 cut(s) 170
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 5 cut(s) 69, 102, 249, 339, 490
AluI AGCT 5 cut(s) 69, 102, 249, 339, 490
Alw21I GWGCWC 2 cut(s) 71, 341
Alw26I GTCTC 2 cut(s) 31, 575
AlwI GGATC 2 cut(s) 24, 370
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 63
Aor51HI AGCGCT 1 cut(s) 365
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 159, 191
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 1 cut(s) 147
AsuC2I CCSGG 2 cut(s) 150, 151
AsuHPI GGTGA 2 cut(s) 210, 312
AsuII TTCGAA 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 149
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 71, 341
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 71, 341
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 272, 325
BceAI ACGGC 1 cut(s) 61
BcnI CCSGG 2 cut(s) 150, 151
BcoDI GTCTC 2 cut(s) 31, 575
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 367
BisI GCNGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 363
Bme1390I CCNGG 2 cut(s) 150, 151
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 520
BmrFI CCNGG 2 cut(s) 150, 151
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 150, 151
BsaJI CCNNGG 2 cut(s) 149, 514
BsaWI WCCGGW 2 cut(s) 63, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 2 cut(s) 42, 589
BseAI TCCGGA 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 149, 514
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMII CTCAG 5 cut(s) 11, 98, 234, 482, 543
BseNI ACTGG 2 cut(s) 42, 589
BseXI GCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 71, 341
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 64, 150, 618
BsiWI CGTACG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 2 cut(s) 31, 575
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 71, 341
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 2 cut(s) 16, 375
BspACI CCGC 1 cut(s) 111
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 5 cut(s) 12, 99, 235, 483, 542
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 520
BspPI GGATC 2 cut(s) 24, 370
BspT104I TTCGAA 1 cut(s) 83
BspT107I GGYRCC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 111
BsrI ACTGG 2 cut(s) 42, 589
BssECI CCNNGG 2 cut(s) 149, 514
BssMI GATC 2 cut(s) 16, 375
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 5 cut(s) 20, 107, 243, 491, 529
BstENI CCTNNNNNAGG 1 cut(s) 331
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 367
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 2 cut(s) 19, 378
BstMAI GTCTC 2 cut(s) 31, 575
BstMBI GATC 2 cut(s) 16, 375
BstMWI GCNNNNNNNGC 3 cut(s) 38, 108, 353
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 148, 149
BstSFI CTRYAG 1 cut(s) 636
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 1 cut(s) 148
BtrI CACGTC 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 35, 543
Cac8I GCNNGC 1 cut(s) 444
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 147
Cfr9I CCCGGG 1 cut(s) 149
CseI GACGC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 48
CviAII CATG 1 cut(s) 443
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 5 cut(s) 20, 107, 243, 491, 529
DpnI GATC 2 cut(s) 18, 377
DpnII GATC 2 cut(s) 16, 375
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Ecl136II GAGCTC 2 cut(s) 69, 339
Eco24I GRGCYC 2 cut(s) 71, 341
Eco47III AGCGCT 1 cut(s) 365
Eco53kI GAGCTC 2 cut(s) 69, 339
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 2 cut(s) 69, 339
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoT38I GRGCYC 2 cut(s) 71, 341
FaeI CATG 1 cut(s) 446
FaiI YATR 3 cut(s) 272, 353, 444
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 647
FatI CATG 1 cut(s) 442
FblI GTMKAC 1 cut(s) 500
Fnu4HI GCNGC 1 cut(s) 362
FriOI GRGCYC 2 cut(s) 71, 341
Fsp4HI GCNGC 1 cut(s) 362
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 365
GluI GCNGC 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 367
HaeIII GGCC 1 cut(s) 148
HapII CCGG 3 cut(s) 64, 150, 618
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 366
Hin1II CATG 1 cut(s) 446
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HincII GTYRAC 2 cut(s) 182, 430
HindII GTYRAC 2 cut(s) 182, 430
HinfI GANTC 4 cut(s) 24, 92, 288, 470
HpaII CCGG 3 cut(s) 64, 150, 618
HphI GGTGA 2 cut(s) 210, 312
Hpy166II GTNNAC 3 cut(s) 182, 430, 501
Hpy188I TCNGA 6 cut(s) 21, 108, 130, 167, 388, 454
Hpy188III TCNNGA 2 cut(s) 64, 330
Hpy8I GTNNAC 3 cut(s) 182, 430, 501
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 79, 327
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 3 cut(s) 347, 398, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 108, 353
HpyF3I CTNAG 5 cut(s) 20, 107, 243, 491, 529
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 1 cut(s) 446
HspAI GCGC 1 cut(s) 364
Kpn2I TCCGGA 1 cut(s) 63
Kzo9I GATC 2 cut(s) 16, 375
LmnI GCTCC 2 cut(s) 66, 336
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 2 cut(s) 216, 532
MalI GATC 2 cut(s) 18, 377
MbiI CCGCTC 1 cut(s) 111
MboI GATC 2 cut(s) 16, 375
MboII GAAGA 6 cut(s) 72, 316, 373, 382, 433, 649
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 71, 341
MluCI AATT 2 cut(s) 132, 570
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 6 cut(s) 107, 263, 337, 389, 403, 524
MroI TCCGGA 1 cut(s) 63
MseI TTAA 4 cut(s) 135, 231, 258, 555
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 3 cut(s) 64, 150, 618
MspR9I CCNGG 2 cut(s) 150, 151
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 38, 108, 353
NciI CCSGG 2 cut(s) 150, 151
NdeII GATC 2 cut(s) 16, 375
NlaIII CATG 1 cut(s) 446
NlaIV GGNNCC 1 cut(s) 520
NmeAIII GCCGAG 2 cut(s) 8, 495
NmuCI GTSAC 2 cut(s) 216, 532
NspI RCATGY 1 cut(s) 446
NspV TTCGAA 1 cut(s) 83
PaeI GCATGC 1 cut(s) 446
PcsI WCGNNNNNNNCGW 3 cut(s) 175, 657, 666
PfeI GAWTC 3 cut(s) 92, 288, 470
Pfl23II CGTACG 1 cut(s) 47
PkrI GCNGC 1 cut(s) 363
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp124BI GAGCTC 2 cut(s) 71, 341
PspLI CGTACG 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 520
PspPI GGNCC 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 490
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 2 cut(s) 71, 341
SaqAI TTAA 4 cut(s) 135, 231, 258, 555
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 2 cut(s) 16, 375
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 150, 151
SduI GDGCHC 2 cut(s) 71, 341
SetI ASST 9 cut(s) 71, 90, 104, 172, 251, 341, 492, 516, 650
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 83
SmaI CCCGGG 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 269
SphI GCATGC 1 cut(s) 446
Sse9I AATT 2 cut(s) 132, 570
SsiI CCGC 1 cut(s) 111
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 2 cut(s) 71, 341
StyD4I CCNGG 2 cut(s) 148, 149
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 172
TaqI TCGA 3 cut(s) 83, 603, 651
TaqII GACCGA 1 cut(s) 405
TasI AATT 2 cut(s) 132, 570
TfiI GAWTC 3 cut(s) 92, 288, 470
Tru1I TTAA 4 cut(s) 135, 231, 258, 555
Tru9I TTAA 4 cut(s) 135, 231, 258, 555
TscAI CASTG 2 cut(s) 42, 543
TseFI GTSAC 2 cut(s) 216, 532
TseI GCWGC 1 cut(s) 361
Tsp45I GTSAC 2 cut(s) 216, 532
TspGWI ACGGA 2 cut(s) 85, 433
TspMI CCCGGG 1 cut(s) 149
TspRI CASTG 2 cut(s) 42, 543
XagI CCTNNNNNAGG 1 cut(s) 331
XbaI TCTAGA 1 cut(s) 329
XceI RCATGY 1 cut(s) 446
XmaI CCCGGG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 500
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.