MD16G1066100.v1.1

Cell division topological specificity factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010355
Physical Location & Seq
Forward (+)
4618103 .. 4618624
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1066100.v1.1.491

Sequence Viewer

Length: 300 bp
ATGATCCTATTCTCTGACCGATGTGCAGTTAGTGATGAGGCTAAAAGATTGATTGTCAACAACATTGTGCATGCTCTGTCAGATTTCGTTGAGATAGAATCGCGAGACAAAGTTCAGCTGAGTGTGTCTGCCGATACTGATGTGGGAACCATTTACTCCGTCACGGTACCTGTAAGACGGGTTAAGCCAGAATACCAGATTGGAGACGAGACTGGAACAATAACCAATATCGAGTACAAAGATACCGGTGACAGTTCTGGTTCTGTTGATGTCAAATTTGATTTCTTCATCCCTGACTAA

Protein Analysis

100

Amino Acids

10.99

Weight (kDa)

4.44

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 1 - 59 2.1e-07 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 166
AccB1I GGYRCC 1 cut(s) 166
AccII CGCG 1 cut(s) 103
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 2 cut(s) 168, 236
AgeI ACCGGT 1 cut(s) 245
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
Alw26I GTCTC 3 cut(s) 99, 198, 203
ApoI RAATTY 1 cut(s) 275
AsiGI ACCGGT 1 cut(s) 245
Asp718I GGTACC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 260
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BanI GGYRCC 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 99, 198, 203
BmiI GGNNCC 2 cut(s) 148, 168
BsaWI WCCGGW 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 166
BshTI ACCGGT 1 cut(s) 245
BsiSI CCGG 1 cut(s) 246
BsmAI GTCTC 3 cut(s) 99, 198, 203
BsmBI CGTCTC 1 cut(s) 198
Bsp143I GATC 1 cut(s) 3
Bsp68I TCGCGA 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 148, 168
BspT107I GGYRCC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 245
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 2 cut(s) 166, 254
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 288
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 3 cut(s) 99, 198, 203
BstMBI GATC 1 cut(s) 3
BstNSI RCATGY 1 cut(s) 74
BstUI CGCG 1 cut(s) 103
BtsCI GGATG 1 cut(s) 288
BtuMI TCGCGA 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 245
Csp6I GTAC 2 cut(s) 167, 235
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 1 cut(s) 71
CviJI RGCY 3 cut(s) 41, 118, 187
CviKI_1 RGCY 3 cut(s) 41, 118, 187
CviQI GTAC 2 cut(s) 167, 235
DdeI CTNAG 1 cut(s) 119
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 1 cut(s) 74
FaiI YATR 1 cut(s) 72
FatI CATG 1 cut(s) 70
FokI GGATG 1 cut(s) 275
HapII CCGG 1 cut(s) 246
Hin1II CATG 1 cut(s) 74
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 16, 82
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 166, 254
HpyCH4V TGCA 2 cut(s) 26, 70
HpyF3I CTNAG 1 cut(s) 119
Hsp92II CATG 1 cut(s) 74
KpnI GGTACC 1 cut(s) 170
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 6 cut(s) 183, 198, 201, 209, 243, 259
MaeIII GTNAC 2 cut(s) 160, 248
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 1 cut(s) 277
MluCI AATT 1 cut(s) 275
MnlI CCTC 1 cut(s) 31
MseI TTAA 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 103
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 2 cut(s) 148, 168
NmuCI GTSAC 2 cut(s) 160, 248
NruI TCGCGA 1 cut(s) 103
NspI RCATGY 1 cut(s) 74
PaeI GCATGC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 148, 168
PvuII CAGCTG 1 cut(s) 118
RruI TCGCGA 1 cut(s) 103
RsaI GTAC 2 cut(s) 168, 236
RsaNI GTAC 2 cut(s) 167, 235
SaqAI TTAA 1 cut(s) 183
Sau3AI GATC 1 cut(s) 3
SetI ASST 2 cut(s) 120, 172
SphI GCATGC 1 cut(s) 74
Sse9I AATT 1 cut(s) 275
TaaI ACNGT 2 cut(s) 166, 254
TaqI TCGA 1 cut(s) 231
TaqII GACCGA 1 cut(s) 33
TasI AATT 1 cut(s) 275
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 2 cut(s) 160, 248
Tsp45I GTSAC 2 cut(s) 160, 248
TspDTI ATGAA 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 148
XapI RAATTY 1 cut(s) 275
XceI RCATGY 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.