Rh4DG371400

Cell division topological specificity factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010355
Physical Location & Seq
Forward (+)
58997090 .. 58998817
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG371400.1

Sequence Viewer

Length: 666 bp
ATGGCTATTATTGGCGATCTGAGAGTCTCGGCTACACTGGCTTCTCACTCTCTCCGGAGCTCTTTGCCGTCTTCAAAGGTAGAATCCGTTAGCTTTCTGAATGGAGGGTCTAGCATTTCTGAAATTAAACCGAAGTGGCCCAGTATGGCATTTGATAGACGTGATATTCGTCAACATTCCAAGCGATCTATTGGAGATTTTCAAATGTCGCCAAACTCCATTAACCAAGATGCTGAGAGCTTCCTCATTAATGCCATCAATATGAGTTTCTTTGAACGATTCAACTTGGCTTGGAAGATATTGTTCCCATCACCAGCCTCTAGAAGGAGCTCTAATGCAAATATAGCCAAGCAGCGCTTGAAGATGATCCTCTTCTCTGACCGATGTGCAGTTAGTGACGAGGCTAAACGGAAGATTGTCAACAACATTGTGCATGCTCTATCAGATTTTGTGGAGATAGAATCGCGCGACAAAGTTCAGCTGAGTGTGTCTACTGATACTGACCTCGGTACCATTTACTCTGTCACGGTGCCTGTAAGACGGGTTAAGCCAGAATATCAAATTGGAGATGAGACTGGAACAATAACAAATATCGAATACAAAGATACTGGAGAAAATTCTGGTTCTGTTGATGTCAGGTTCGATTTCTTTGTCCCTGACGAATGA

Protein Analysis

221

Amino Acids

24.74

Weight (kDa)

6.85

Isoelectric Point (pI)

61.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 113 - 180 9.9e-14 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 2 cut(s) 509, 529
AccI GTMKAC 1 cut(s) 491
AccII CGCG 2 cut(s) 466, 468
AccIII TCCGGA 1 cut(s) 54
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 1 cut(s) 616
AfaI GTAC 1 cut(s) 511
AfeI AGCGCT 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 5 cut(s) 75, 203, 275, 283, 361
AjiI CACGTC 1 cut(s) 161
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
AluBI AGCT 5 cut(s) 60, 93, 240, 330, 481
AluI AGCT 5 cut(s) 60, 93, 240, 330, 481
Alw21I GWGCWC 2 cut(s) 62, 332
Alw26I GTCTC 2 cut(s) 31, 566
AlwI GGATC 1 cut(s) 361
Aor13HI TCCGGA 1 cut(s) 54
Aor51HI AGCGCT 1 cut(s) 356
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 1 cut(s) 616
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
AseI ATTAAT 1 cut(s) 249
Asp718I GGTACC 1 cut(s) 509
AspLEI GCGC 2 cut(s) 357, 468
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 303
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BanI GGYRCC 2 cut(s) 509, 529
BanII GRGCYC 2 cut(s) 62, 332
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 62, 332
BbvI GCAGC 1 cut(s) 364
BccI CCATC 2 cut(s) 263, 316
BceAI ACGGC 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 31, 566
BfaI CTAG 2 cut(s) 111, 321
BfoI RGCGCY 1 cut(s) 358
BisI GCNGC 1 cut(s) 353
BlsI GCNGC 1 cut(s) 354
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 2 cut(s) 511, 531
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 220
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 630
BsaJI CCNNGG 1 cut(s) 505
BsaWI WCCGGW 1 cut(s) 54
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse1I ACTGG 4 cut(s) 42, 141, 580, 613
BseAI TCCGGA 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 505
BseLI CCNNNNNNNGG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 11, 225, 473
BseNI ACTGG 4 cut(s) 42, 141, 580, 613
BseXI GCAGC 1 cut(s) 364
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 466, 468
BshFI GGCC 1 cut(s) 139
BshNI GGYRCC 2 cut(s) 509, 529
BsiHKAI GWGCWC 2 cut(s) 62, 332
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 1 cut(s) 324
BsmAI GTCTC 2 cut(s) 31, 566
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 1 cut(s) 139
Bsp1286I GDGCHC 2 cut(s) 62, 332
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 3 cut(s) 16, 185, 366
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 3 cut(s) 12, 226, 474
BspEI TCCGGA 1 cut(s) 54
BspFNI CGCG 2 cut(s) 466, 468
BspLI GGNNCC 2 cut(s) 511, 531
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 2 cut(s) 509, 529
BsrI ACTGG 4 cut(s) 42, 141, 580, 613
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 3 cut(s) 16, 185, 366
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 20, 234, 482
BstENI CCTNNNNNAGG 1 cut(s) 322
BstFNI CGCG 2 cut(s) 466, 468
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 2 cut(s) 357, 468
BstKTI GATC 3 cut(s) 19, 188, 369
BstMAI GTCTC 2 cut(s) 31, 566
BstMBI GATC 3 cut(s) 16, 185, 366
BstMWI GCNNNNNNNGC 2 cut(s) 38, 344
BstNSI RCATGY 1 cut(s) 437
BstUI CGCG 2 cut(s) 466, 468
BstV1I GCAGC 1 cut(s) 364
BstV2I GAAGAC 1 cut(s) 63
BsuRI GGCC 1 cut(s) 139
BtrI CACGTC 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 2 cut(s) 357, 468
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 510
CviAII CATG 1 cut(s) 434
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 3 cut(s) 20, 234, 482
DpnI GATC 3 cut(s) 18, 187, 368
DpnII GATC 3 cut(s) 16, 185, 366
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Ecl136II GAGCTC 2 cut(s) 60, 330
Eco24I GRGCYC 2 cut(s) 62, 332
Eco47III AGCGCT 1 cut(s) 356
Eco53kI GAGCTC 2 cut(s) 60, 330
EcoICRI GAGCTC 2 cut(s) 60, 330
EcoNI CCTNNNNNAGG 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 62, 332
FaeI CATG 1 cut(s) 437
FaiI YATR 4 cut(s) 146, 263, 344, 435
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 638
FatI CATG 1 cut(s) 433
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 1 cut(s) 353
FriOI GRGCYC 2 cut(s) 62, 332
Fsp4HI GCNGC 1 cut(s) 353
FspBI CTAG 2 cut(s) 111, 321
GlaI GCGC 2 cut(s) 356, 467
GluI GCNGC 1 cut(s) 353
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 358
HaeIII GGCC 1 cut(s) 139
HapII CCGG 1 cut(s) 55
HhaI GCGC 2 cut(s) 357, 468
Hin1II CATG 1 cut(s) 437
Hin6I GCGC 2 cut(s) 355, 466
HinP1I GCGC 2 cut(s) 355, 466
HincII GTYRAC 2 cut(s) 173, 421
HindII GTYRAC 2 cut(s) 173, 421
HinfI GANTC 4 cut(s) 24, 83, 279, 461
HpaII CCGG 1 cut(s) 55
HphI GGTGA 1 cut(s) 303
Hpy166II GTNNAC 3 cut(s) 173, 421, 492
Hpy188I TCNGA 5 cut(s) 21, 99, 121, 379, 445
Hpy188III TCNNGA 2 cut(s) 55, 321
Hpy8I GTNNAC 3 cut(s) 173, 421, 492
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 338, 389, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 344
HpyF3I CTNAG 3 cut(s) 20, 234, 482
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 1 cut(s) 437
HspAI GCGC 2 cut(s) 355, 466
Kpn2I TCCGGA 1 cut(s) 54
KpnI GGTACC 1 cut(s) 513
Kzo9I GATC 3 cut(s) 16, 185, 366
LmnI GCTCC 2 cut(s) 57, 327
Lsp1109I GCAGC 1 cut(s) 364
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 111, 321
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 395, 523
MalI GATC 3 cut(s) 18, 187, 368
MboI GATC 3 cut(s) 16, 185, 366
MboII GAAGA 5 cut(s) 63, 307, 364, 373, 424
MhlI GDGCHC 2 cut(s) 62, 332
MluCI AATT 3 cut(s) 123, 561, 616
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 6 cut(s) 98, 254, 328, 380, 394, 515
MroI TCCGGA 1 cut(s) 54
MseI TTAA 4 cut(s) 126, 222, 249, 546
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 481
MspI CCGG 1 cut(s) 55
MvnI CGCG 2 cut(s) 466, 468
MwoI GCNNNNNNNGC 2 cut(s) 38, 344
NdeII GATC 3 cut(s) 16, 185, 366
NlaIII CATG 1 cut(s) 437
NlaIV GGNNCC 2 cut(s) 511, 531
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 395, 523
NspI RCATGY 1 cut(s) 437
PaeI GCATGC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PfeI GAWTC 3 cut(s) 83, 279, 461
PkrI GCNGC 1 cut(s) 354
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PshBI ATTAAT 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 62, 332
PspN4I GGNNCC 2 cut(s) 511, 531
PspPI GGNCC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 481
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 2 cut(s) 62, 332
SaqAI TTAA 4 cut(s) 126, 222, 249, 546
SatI GCNGC 1 cut(s) 353
Sau3AI GATC 3 cut(s) 16, 185, 366
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 33
SduI GDGCHC 2 cut(s) 62, 332
SetI ASST 9 cut(s) 62, 81, 95, 163, 242, 332, 483, 507, 641
SfaNI GCATC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 260
SphI GCATGC 1 cut(s) 437
Sse9I AATT 3 cut(s) 123, 561, 616
SspMI CTAG 2 cut(s) 111, 321
SstI GAGCTC 2 cut(s) 62, 332
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 1 cut(s) 163
TaqI TCGA 2 cut(s) 594, 642
TaqII GACCGA 1 cut(s) 396
TasI AATT 3 cut(s) 123, 561, 616
TfiI GAWTC 3 cut(s) 83, 279, 461
Tru1I TTAA 4 cut(s) 126, 222, 249, 546
Tru9I TTAA 4 cut(s) 126, 222, 249, 546
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 2 cut(s) 395, 523
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 2 cut(s) 395, 523
TspGWI ACGGA 2 cut(s) 76, 424
TspRI CASTG 1 cut(s) 42
VspI ATTAAT 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 322
XapI RAATTY 1 cut(s) 616
XbaI TCTAGA 1 cut(s) 320
XceI RCATGY 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 491
XspI CTAG 2 cut(s) 111, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.