FvH4_4g31720

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005350
Physical Location & Seq
Reverse (-)
30746043 .. 30751488
5446 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31720.t2

Sequence Viewer

Length: 1797 bp
ATGGGGAAAACGCCGGGGAAATGGATCAAGAATTTGCTTCTTGGGAAGAAATCGTCCAAAGGAAGAGAGAAATCTGCAAGTAAAGGGGAGTCGGTGTTCTCTTCAAAGCTGTCTGTGCCGGAGTTCACTGTCAGTGCACCTGTGATATCACTACCATTGGTTGCGACGGTTGTTAGTAACGGAGTGGACTCAGAAATCAGAGCAGCTGCAGAAGTACCGAATGATAACATTACACTTTCGTCGGGAAAGGATGATGGACAGACACAGGCTATCTCTAGTTCGGGGTCTCAGGATCTTCGGAAGCGAGAGGAAGCGGCCACAAAGGCGCAGAGTGCTTATAGAGGATATGTGGCTCGCCGTGCATATCGAACCCTGAAGGGCATCATAAGGCTGCAAGCACTTATTCGTGGTCATTTGGTCAGAAGACAAGCTGTATCCACATTGTTATGTGTCCAGGGGATTGTGAAGTTTCAAGCACTGATTCGTGGTCAGCATGTTAGACATTCGGATATTGGAGTTGAAGTGCATAAAATACATCAGGGTGATACATGTTTCGGTTACTCTGGTGAAACTGCATCGTCCCAAGTGGAGAGGCTTCCAAAAAATGCTTTTGCTCAGAAGCTTCTGGCATCATTGCCTACTGCTAGTCCTCTACGTCTCCAGTATGGTCCAGAGGATCCGAACTCGTCCGGGGTGTGGCTTGACCGGTGGACCAGATCATGCTTTTGGGAACCTGTTTTACAACTAAAGAAAAAACCTGATCCGAAATCTCGTGGCAAATACAAGAAGATTCAAACAGTAGAAACTGAACCAGCTAAGCCAAAAAGAACTGTCCGCAGACTATCCAATGAAAATGGGCCAAATCGTGTCACTTCAGATTCTGAAAAGCCTAAACGAATTATGAGGAAAGTTTCAAGTCATCCAGTGAACTCAGTTCAGGAACATCAACAGAATGGAGTTGAGAAGCACAAAAGTAATGTTAGAAAAGTATCTGATCCCAAGGAGGTTTCTAATTCCAAGAAGGTTTCTGATCCCAAGAAGAAGGTTTCTGATCCCAAGGAGGAGGTTTCTGGTTCCAAGAAGAAGGTTTCTGATTCCAAGGAGGAGGTTTCTGATCGGTCAGAGGTTGATAAGGAGATACCGAAGCACAGTACAGAGATAGTTATAGCCCCTGCTCTTGATGATGCAGAGCAGCGCACTAGCGAGAGTTCTCCAAAGATGCAACATGTGGAAGTTTCTGTCTCCAAACACTCTGATCCAGAATGTATGGAGTTGACACCAACAAATGAGACATTGCAAAAGTTAGATGACCATTCTTCTGGTGACATGCAATCCATGGAGAACAATGGCAAAAATGAAGAAATTCAACCACTAAATGAGGTTCTAACCTCTGCAGACAACTTTATTAGCAATGAGGAACAAAAACACAGCCAGAGAAGGGCTTCCTTACCTGTGAAGTTTGATCATCGGGAGAATGGGCTTCAGACCACACCAAGAGTTCCAAGTTACATGGCACCAACTGAATCTGCCAGAGTTCCAAGTTACATGGCACCAACTGAATCTGCCAGAGCTAGGCTGAAAGGACAGGGCTCCCCCAGGTTTGCACGAGACGTGGTTGAGAAGAATCTTTTAACCAGACGGCATTCTCTGTCTTCTTCCACCAACACCAAGTTAAGTTTACTTTCTCCTCGAGCACAAAAATTGGTTCAAGGCAAAGGAGTGATCAGGACCGACAGATCTTTATCATCTTCAAGGGATGGTTTTGATAAGGGGATCCAACCTGAGTGGAGGAGGTGA

Protein Analysis

599

Amino Acids

66.36

Weight (kDa)

9.8

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 491 - 562 9.1e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1513, 1549
AciI CCGC 2 cut(s) 314, 835
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 2 cut(s) 31, 1362
AcuI CTGAAG 3 cut(s) 395, 858, 1466
AfaI GTAC 2 cut(s) 216, 1153
AfiI CCNNNNNNNGG 3 cut(s) 696, 1438, 1572
AflIII ACRYGT 2 cut(s) 548, 1225
AgeI ACCGGT 1 cut(s) 705
AgsI TTSAA 8 cut(s) 105, 473, 521, 794, 915, 1367, 1709, 1752
AjiI CACGTC 1 cut(s) 1612
AjnI CCWGG 2 cut(s) 453, 1595
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 6 cut(s) 109, 206, 431, 622, 815, 1571
AluI AGCT 6 cut(s) 109, 206, 431, 622, 815, 1571
Alw21I GWGCWC 2 cut(s) 139, 1696
Alw26I GTCTC 5 cut(s) 291, 662, 1246, 1283, 1602
Alw44I GTGCAC 1 cut(s) 135
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 1 cut(s) 1689
AoxI GGCC 2 cut(s) 315, 857
ApaLI GTGCAC 1 cut(s) 135
ApeKI GCWGC 4 cut(s) 203, 206, 391, 1192
ApoI RAATTY 2 cut(s) 31, 1362
AsiGI ACCGGT 1 cut(s) 705
Asp700I GAANNNNTTC 2 cut(s) 1362, 1441
AspLEI GCGC 2 cut(s) 328, 1197
AspS9I GGNCC 4 cut(s) 668, 711, 857, 1728
AsuC2I CCSGG 2 cut(s) 15, 691
AsuHPI GGTGA 3 cut(s) 554, 578, 1334
AvaI CYCGRG 1 cut(s) 1689
AvaII GGWCC 3 cut(s) 668, 711, 1728
BaeGI GKGCMC 1 cut(s) 139
BamHI GGATCC 2 cut(s) 676, 1773
BanI GGYRCC 2 cut(s) 1513, 1549
BanII GRGCYC 1 cut(s) 1592
BauI CACGAG 2 cut(s) 771, 1605
BbsI GAAGAC 2 cut(s) 430, 1644
Bbv12I GWGCWC 2 cut(s) 139, 1696
BbvI GCAGC 4 cut(s) 193, 215, 378, 1204
BccI CCATC 2 cut(s) 248, 1751
BceAI ACGGC 2 cut(s) 342, 1655
BciT130I CCWGG 2 cut(s) 455, 1597
BciVI GTATCC 1 cut(s) 445
BclI TGATCA 2 cut(s) 1462, 1722
BcnI CCSGG 2 cut(s) 15, 691
BcoDI GTCTC 5 cut(s) 291, 662, 1246, 1283, 1602
BfaI CTAG 4 cut(s) 276, 645, 1200, 1572
BfmI CTRYAG 2 cut(s) 207, 1392
BfuI GTATCC 1 cut(s) 445
BglI GCCNNNNNGGC 1 cut(s) 323
BglII AGATCT 1 cut(s) 1736
BisI GCNGC 5 cut(s) 204, 207, 315, 392, 1193
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 5 cut(s) 205, 208, 316, 393, 1194
Bme1390I CCNGG 4 cut(s) 15, 455, 691, 1597
Bme18I GGWCC 3 cut(s) 668, 711, 1728
BmeT110I CYCGRG 1 cut(s) 1689
BmgBI CACGTC 1 cut(s) 1612
BmgT120I GGNCC 4 cut(s) 668, 711, 857, 1728
BmiI GGNNCC 7 cut(s) 678, 732, 1075, 1515, 1551, 1591, 1775
BmrFI CCNGG 4 cut(s) 15, 455, 691, 1597
BmsI GCATC 5 cut(s) 390, 584, 638, 1174, 1209
BpiI GAAGAC 2 cut(s) 430, 1644
BpmI CTGGAG 1 cut(s) 644
Bpu1102I GCTNAGC 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 15, 691
BsaBI GATNNNNATC 1 cut(s) 1741
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 8 cut(s) 14, 454, 690, 999, 1056, 1098, 1335, 1595
BsaWI WCCGGW 1 cut(s) 705
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 696, 1438, 1572
Bse118I RCCGGY 1 cut(s) 705
Bse1I ACTGG 2 cut(s) 661, 923
Bse3DI GCAATG 3 cut(s) 632, 1292, 1417
Bse8I GATNNNNATC 1 cut(s) 1741
BseBI CCWGG 2 cut(s) 455, 1597
BseDI CCNNGG 8 cut(s) 14, 454, 690, 999, 1056, 1098, 1335, 1595
BseGI GGATG 3 cut(s) 256, 919, 1762
BseJI GATNNNNATC 1 cut(s) 1741
BseLI CCNNNNNNNGG 3 cut(s) 696, 1438, 1572
BseMI GCAATG 3 cut(s) 632, 1292, 1417
BseMII CTCAG 5 cut(s) 204, 302, 629, 945, 1773
BseNI ACTGG 2 cut(s) 661, 923
BseRI GAGGAG 3 cut(s) 1076, 1118, 1677
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 4 cut(s) 193, 215, 378, 1204
BshFI GGCC 2 cut(s) 317, 859
BshNI GGYRCC 2 cut(s) 1513, 1549
BshTI ACCGGT 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 139, 1696
BsiHKCI CYCGRG 1 cut(s) 1689
BsiSI CCGG 4 cut(s) 14, 119, 690, 706
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 3 cut(s) 696, 1438, 1572
BsmAI GTCTC 5 cut(s) 291, 662, 1246, 1283, 1602
BsmBI CGTCTC 2 cut(s) 662, 1602
BsmFI GGGAC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 1642
BsnI GGCC 2 cut(s) 317, 859
Bso31I GGTCTC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 1689
Bsp1286I GDGCHC 3 cut(s) 139, 1592, 1696
Bsp1720I GCTNAGC 1 cut(s) 816
Bsp19I CCATGG 1 cut(s) 1335
BspACI CCGC 2 cut(s) 314, 835
BspANI GGCC 2 cut(s) 317, 859
BspCNI CTCAG 5 cut(s) 203, 301, 628, 944, 1774
BspLI GGNNCC 7 cut(s) 678, 732, 1075, 1515, 1551, 1591, 1775
BspMAI CTGCAG 2 cut(s) 211, 1396
BspT107I GGYRCC 2 cut(s) 1513, 1549
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 3 cut(s) 632, 1292, 1417
BsrFI RCCGGY 1 cut(s) 705
BsrI ACTGG 2 cut(s) 661, 923
BssAI RCCGGY 1 cut(s) 705
BssECI CCNNGG 8 cut(s) 14, 454, 690, 999, 1056, 1098, 1335, 1595
BssSI CACGAG 2 cut(s) 771, 1605
BssT1I CCWWGG 4 cut(s) 999, 1056, 1098, 1335
Bst2BI CACGAG 2 cut(s) 771, 1605
Bst2UI CCWGG 2 cut(s) 455, 1597
Bst4CI ACNGT 5 cut(s) 130, 169, 799, 832, 1151
Bst6I CTCTTC 2 cut(s) 58, 106
BstC8I GCNNGC 2 cut(s) 355, 396
BstDEI CTNAG 6 cut(s) 190, 288, 615, 816, 931, 1782
BstDSI CCRYGG 1 cut(s) 1335
BstF5I GGATG 3 cut(s) 256, 919, 1762
BstHHI GCGC 2 cut(s) 328, 1197
BstMAI GTCTC 5 cut(s) 291, 662, 1246, 1283, 1602
BstMWI GCNNNNNNNGC 4 cut(s) 115, 323, 332, 359
BstNI CCWGG 2 cut(s) 455, 1597
BstNSI RCATGY 4 cut(s) 497, 552, 1229, 1330
BstSCI CCNGG 4 cut(s) 13, 453, 689, 1595
BstSFI CTRYAG 2 cut(s) 207, 1392
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 4 cut(s) 193, 215, 378, 1204
BstV2I GAAGAC 2 cut(s) 430, 1644
BstX2I RGATCY 4 cut(s) 292, 676, 1736, 1773
BstXI CCANNNNNNTGG 1 cut(s) 1319
BstYI RGATCY 4 cut(s) 292, 676, 1736, 1773
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 2 cut(s) 317, 859
BtgI CCRYGG 1 cut(s) 1335
BtrI CACGTC 1 cut(s) 1612
BtsCI GGATG 3 cut(s) 256, 919, 1762
BtsIMutI CAGTG 4 cut(s) 126, 139, 476, 930
Cac8I GCNNGC 2 cut(s) 355, 396
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 2 cut(s) 328, 1197
Cfr10I RCCGGY 1 cut(s) 705
Cfr13I GGNCC 4 cut(s) 668, 711, 857, 1728
Csp6I GTAC 2 cut(s) 215, 1152
CspAI ACCGGT 1 cut(s) 705
CspCI CAANNNNNGTGG 1 cut(s) 1766
CviAII CATG 8 cut(s) 494, 549, 720, 1226, 1327, 1336, 1510, 1546
CviQI GTAC 2 cut(s) 215, 1152
DdeI CTNAG 6 cut(s) 190, 288, 615, 816, 931, 1782
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 58, 106
EarI CTCTTC 2 cut(s) 58, 106
Eco130I CCWWGG 4 cut(s) 999, 1056, 1098, 1335
Eco24I GRGCYC 1 cut(s) 1592
Eco31I GGTCTC 1 cut(s) 291
Eco32I GATATC 1 cut(s) 147
Eco47I GGWCC 3 cut(s) 668, 711, 1728
Eco57I CTGAAG 3 cut(s) 395, 858, 1466
Eco88I CYCGRG 1 cut(s) 1689
EcoRII CCWGG 2 cut(s) 453, 1595
EcoRV GATATC 1 cut(s) 147
EcoT14I CCWWGG 4 cut(s) 999, 1056, 1098, 1335
EcoT38I GRGCYC 1 cut(s) 1592
ErhI CCWWGG 4 cut(s) 999, 1056, 1098, 1335
Esp3I CGTCTC 2 cut(s) 662, 1602
FaeI CATG 8 cut(s) 497, 552, 723, 1229, 1330, 1339, 1513, 1549
FaqI GGGAC 1 cut(s) 565
FatI CATG 8 cut(s) 493, 548, 719, 1225, 1326, 1335, 1509, 1545
FbaI TGATCA 2 cut(s) 1462, 1722
Fnu4HI GCNGC 5 cut(s) 204, 207, 315, 392, 1193
FokI GGATG 3 cut(s) 263, 906, 1769
FriOI GRGCYC 1 cut(s) 1592
Fsp4HI GCNGC 5 cut(s) 204, 207, 315, 392, 1193
FspBI CTAG 4 cut(s) 276, 645, 1200, 1572
GlaI GCGC 2 cut(s) 327, 1196
GluI GCNGC 5 cut(s) 204, 207, 315, 392, 1193
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 2 cut(s) 317, 859
HapII CCGG 4 cut(s) 14, 119, 690, 706
HhaI GCGC 2 cut(s) 328, 1197
Hin1II CATG 8 cut(s) 497, 552, 723, 1229, 1330, 1339, 1513, 1549
Hin6I GCGC 2 cut(s) 326, 1195
HinP1I GCGC 2 cut(s) 326, 1195
HincII GTYRAC 1 cut(s) 1275
HindII GTYRAC 1 cut(s) 1275
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 9 cut(s) 89, 188, 481, 790, 878, 1094, 1523, 1559, 1624
HpaII CCGG 4 cut(s) 14, 119, 690, 706
HphI GGTGA 3 cut(s) 554, 578, 1334
Hpy166II GTNNAC 7 cut(s) 126, 137, 187, 711, 928, 1275, 1679
Hpy188III TCNNGA 9 cut(s) 28, 243, 290, 671, 938, 1178, 1259, 1469, 1726
Hpy8I GTNNAC 7 cut(s) 126, 137, 187, 711, 928, 1275, 1679
Hpy99I CGWCG 2 cut(s) 169, 244
HpyAV CCTTC 5 cut(s) 370, 1015, 1036, 1078, 1431
HpyCH4III ACNGT 5 cut(s) 130, 169, 799, 832, 1151
HpyCH4IV ACGT 2 cut(s) 655, 1611
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 323, 332, 359
HpyF3I CTNAG 6 cut(s) 190, 288, 615, 816, 931, 1782
HpySE526I ACGT 2 cut(s) 655, 1611
Hsp92II CATG 8 cut(s) 497, 552, 723, 1229, 1330, 1339, 1513, 1549
HspAI GCGC 2 cut(s) 326, 1195
Ksp22I TGATCA 2 cut(s) 1462, 1722
LmnI GCTCC 1 cut(s) 1595
Lsp1109I GCAGC 4 cut(s) 193, 215, 378, 1204
LweI GCATC 5 cut(s) 390, 584, 638, 1174, 1209
MaeI CTAG 4 cut(s) 276, 645, 1200, 1572
MaeII ACGT 2 cut(s) 655, 1611
MaeIII GTNAC 6 cut(s) 176, 557, 868, 1322, 1505, 1541
MflI RGATCY 4 cut(s) 292, 676, 1736, 1773
MhlI GDGCHC 3 cut(s) 139, 1592, 1696
MluCI AATT 5 cut(s) 31, 897, 1012, 1362, 1700
MlyI GAGTC 2 cut(s) 98, 182
MroXI GAANNNNTTC 2 cut(s) 1362, 1441
MseI TTAA 2 cut(s) 1631, 1673
MslI CAYNNNNRTG 2 cut(s) 445, 540
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 4 cut(s) 14, 119, 690, 706
MspR9I CCNGG 4 cut(s) 15, 455, 691, 1597
Mva1269I GAATGC 1 cut(s) 1642
MvaI CCWGG 2 cut(s) 455, 1597
MwoI GCNNNNNNNGC 4 cut(s) 115, 323, 332, 359
NciI CCSGG 2 cut(s) 15, 691
NcoI CCATGG 1 cut(s) 1335
NlaIII CATG 8 cut(s) 497, 552, 723, 1229, 1330, 1339, 1513, 1549
NlaIV GGNNCC 7 cut(s) 678, 732, 1075, 1515, 1551, 1591, 1775
NmuCI GTSAC 2 cut(s) 868, 1322
NspI RCATGY 4 cut(s) 497, 552, 1229, 1330
PaeR7I CTCGAG 1 cut(s) 1689
PciI ACATGT 2 cut(s) 548, 1225
PctI GAATGC 1 cut(s) 1642
PdmI GAANNNNTTC 2 cut(s) 1362, 1441
PfeI GAWTC 7 cut(s) 481, 790, 878, 1094, 1523, 1559, 1624
PinAI ACCGGT 1 cut(s) 705
PkrI GCNGC 5 cut(s) 205, 208, 316, 393, 1194
PleI GAGTC 2 cut(s) 97, 182
PpsI GAGTC 2 cut(s) 97, 182
PscI ACATGT 2 cut(s) 548, 1225
Psp6I CCWGG 2 cut(s) 453, 1595
PspGI CCWGG 2 cut(s) 453, 1595
PspN4I GGNNCC 7 cut(s) 678, 732, 1075, 1515, 1551, 1591, 1775
PspPI GGNCC 4 cut(s) 668, 711, 857, 1728
PspXI VCTCGAGB 1 cut(s) 1689
PsrI GAACNNNNNNTAC 2 cut(s) 723, 755
PstI CTGCAG 2 cut(s) 211, 1396
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 4 cut(s) 292, 676, 1736, 1773
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 216, 1153
RsaNI GTAC 2 cut(s) 215, 1152
RseI CAYNNNNRTG 2 cut(s) 445, 540
SaqAI TTAA 2 cut(s) 1631, 1673
SatI GCNGC 5 cut(s) 204, 207, 315, 392, 1193
Sau96I GGNCC 4 cut(s) 668, 711, 857, 1728
SchI GAGTC 2 cut(s) 98, 182
ScrFI CCNGG 4 cut(s) 15, 455, 691, 1597
SduI GDGCHC 3 cut(s) 139, 1592, 1696
SfaNI GCATC 5 cut(s) 390, 584, 638, 1174, 1209
SfcI CTRYAG 2 cut(s) 207, 1392
Sfr274I CTCGAG 1 cut(s) 1689
SinI GGWCC 3 cut(s) 668, 711, 1728
SlaI CTCGAG 1 cut(s) 1689
SmiMI CAYNNNNRTG 2 cut(s) 445, 540
SmlI CTYRAG 1 cut(s) 1689
SmoI CTYRAG 1 cut(s) 1689
Sse9I AATT 5 cut(s) 31, 897, 1012, 1362, 1700
SsiI CCGC 2 cut(s) 314, 835
SspMI CTAG 4 cut(s) 276, 645, 1200, 1572
StyD4I CCNGG 4 cut(s) 13, 453, 689, 1595
StyI CCWWGG 4 cut(s) 999, 1056, 1098, 1335
TaaI ACNGT 5 cut(s) 130, 169, 799, 832, 1151
TaiI ACGT 2 cut(s) 658, 1614
TaqI TCGA 2 cut(s) 367, 1690
TaqII GACCGA 2 cut(s) 1107, 1745
TasI AATT 5 cut(s) 31, 897, 1012, 1362, 1700
TatI WGTACW 1 cut(s) 1151
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 7 cut(s) 481, 790, 878, 1094, 1523, 1559, 1624
Tru1I TTAA 2 cut(s) 1631, 1673
Tru9I TTAA 2 cut(s) 1631, 1673
TscAI CASTG 4 cut(s) 133, 139, 483, 930
TseFI GTSAC 2 cut(s) 868, 1322
TseI GCWGC 4 cut(s) 203, 206, 391, 1192
Tsp45I GTSAC 2 cut(s) 868, 1322
TspDTI ATGAA 2 cut(s) 864, 1371
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 4 cut(s) 133, 139, 483, 930
VneI GTGCAC 1 cut(s) 135
VpaK11BI GGWCC 3 cut(s) 668, 711, 1728
XapI RAATTY 2 cut(s) 31, 1362
XceI RCATGY 4 cut(s) 497, 552, 1229, 1330
XhoI CTCGAG 1 cut(s) 1689
XmnI GAANNNNTTC 2 cut(s) 1362, 1441
XspI CTAG 4 cut(s) 276, 645, 1200, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.