Rh4BG389500

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005350
Physical Location & Seq
Reverse (-)
54285397 .. 54289383
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG389500.1

Sequence Viewer

Length: 1785 bp
ATGGGAAAAACGCCGGGAAAATGGATCAAGAATTTGCTTCTTGGGAAGAAATCGTCCAGGTCTAGTTTGTCCAAAGGAAGAGAGAAATCTGCAAATAAAGGGGAGACAGTGGTCTCTTCAAAGTTGTCTGTGCCTGAGTTAACTGTCAGTGCACCTGTGATATCACCACCATTGGTTGGGACGGTTGTTAGTAACGTGGACTCAGAAATCAGAGCAGCTGTTGAAATACCAAACGATAACATTACACTTTCATCAGCAAAGGAAGATGGCCAAACACAAGCTATCTCTAGTTCGGGGTCTCAGGATCTTCATAGAATTAAGCGAGAGGAAGCTGCCACAAAGGCACAGAGTGCTTATAGAGGATATGTGGCTCGCCGTGCATATCGAACCCTGAAGGGCATCATAAGGCTGCAAGCACTTATTCGTGGTCATTTGGTTAGAAGACAAGCTGTATCCACATTATTATGTGTCCAGGGGATTGTGAAGTTTCAAGCACTGATTCGTGGTCAGCATGTTAGACATTCTGATATTGGGGTTGAAGTGCATAAAACACATCAGGGTGCTACATGTTTCGATTACTCTGGTGTAAGTGCATCTTCCCAGGTGGACAGGCTTTCGAAAAATGCATTTGCTCAGAAGCTTCTAGCATCATTGCCTACTGCTAGTCCTCTACGTCTCCAGTATGATCCAGAGGACCCGAACTCAGCCTGGGTGTGGCTTGACCGCTGGACCAGATCATGCTTTTGGGAACCTGTTTTACAACTAAAGAAAAAACCTGATCCGAAGTCTCGTGGCAAATACAAGAAGATTCAAACAGTAGAAACTGAACCGGCTGAGCCAAAAAGAACTGTCCGGAGACTGTCCACTGAAAATGGGCCAAATCGTGTCACTTCAGATACTGAAAAGCCTAAACGAAATATGAGGAAAGTTTCAAGTCATCCAATGAACTCAGTTCAGGAACATCAACAAAACGGAGTTGAGAAGGTCAAAAGTAAGGTCAGAAAAGTTTCTGGTCCCAAGGAGGTTTCCAATTCCAAAAAGGTTTCTGATCCCAAGGAGGAGGTTTCTGATCCCAAGGAGGAGGTTTCTGATACCAAGGAGGAGGTTTCTGATTCCAAGGAGGAGGTTTCTGACCGGCCAGAGGTTGATAAGGAGATACCAAAGCACAGTACAGAAACAATTTTAGCTCCTGCTGTTCTTGATTATTCAGAGCAGCACACTAGTGAGATTTCTCCGAAGATGCAACATGTGGAAGTTTCTGTCTCCAAACACTCTGATCTAGAAGAATGTATGGAGCTGCCACCAACAGATGAGACATTGCAAAAGTTAGATGACCATCCTTCTGTTGACATGCAATCCATGGAGAAGAATGGCAAAAATGAAGAAGTTCAAGCACTAAATGAGGTTCTAACCTCTGCAGACAACTTTATTAGCAACGAGGACCAGAAAACCAGCCAGAGAAGGGCTTCTTTACCTGTGAAGTTTGATCATCGGGATAATGGGGTTCAAACCACACCAAGAGTGCCAAGTTACATGGCACCAACTGAATCTGCCAGAGCTAGGCTGAAAGGACAGAGCTCTCCCAGGTTTGCACGAGACGTGATTGATAAGAATCTTTTAACCAGACGGCATTCTCTGTCTTCTTCCACCAACACCAAGTTAAGTTTACTTTCTCCACGAGCACAAAAATTGGCTCAAGGCAAAGGAGTGATCAGAACTGACAGATCTTTATCATCTTCAAGGGATGGTTTTGATAAGGGGATCCAACCTGAGTGGAGGAGGTGA

Protein Analysis

594

Amino Acids

66.2

Weight (kDa)

9.57

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 497 - 558 2.3e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1537
AccB7I CCANNNNNTGG 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 852
AciI CCGC 1 cut(s) 724
AclWI GGATC 8 cut(s) 32, 312, 680, 773, 1043, 1064, 1756, 1769
AcoI YGGCCR 2 cut(s) 268, 1136
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 2 cut(s) 413, 876
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 1 cut(s) 1171
AfiI CCNNNNNNNGG 5 cut(s) 176, 714, 1141, 1462, 1560
AflIII ACRYGT 2 cut(s) 566, 1246
AgsI TTSAA 9 cut(s) 120, 224, 491, 539, 812, 933, 1391, 1508, 1740
AhlI ACTAGT 1 cut(s) 1220
AjiI CACGTC 1 cut(s) 1600
AjnI CCWGG 5 cut(s) 56, 471, 600, 707, 1583
AleI CACNNNNGTG 1 cut(s) 1221
AluBI AGCT 9 cut(s) 218, 281, 332, 449, 640, 1187, 1297, 1559, 1578
AluI AGCT 9 cut(s) 218, 281, 332, 449, 640, 1187, 1297, 1559, 1578
Alw21I GWGCWC 3 cut(s) 154, 1580, 1684
Alw26I GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1267, 1307, 1590
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 8 cut(s) 32, 312, 680, 773, 1043, 1064, 1756, 1769
AlwNI CAGNNNCTG 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 852
AoxI GGCC 3 cut(s) 268, 875, 1136
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 5 cut(s) 215, 332, 409, 1213, 1297
ApoI RAATTY 1 cut(s) 31
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
Asp700I GAANNNNTTC 3 cut(s) 1006, 1386, 1465
AspS9I GGNCC 5 cut(s) 694, 729, 875, 1013, 1441
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 156
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 4 cut(s) 694, 729, 1013, 1441
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 1761
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 1 cut(s) 1580
BauI CACGAG 3 cut(s) 789, 1593, 1677
BbsI GAAGAC 2 cut(s) 448, 1632
Bbv12I GWGCWC 3 cut(s) 154, 1580, 1684
BbvI GCAGC 5 cut(s) 227, 319, 396, 1225, 1284
BccI CCATC 3 cut(s) 260, 1344, 1739
BceAI ACGGC 2 cut(s) 360, 1643
BciT130I CCWGG 5 cut(s) 58, 473, 602, 709, 1585
BciVI GTATCC 1 cut(s) 463
BclI TGATCA 2 cut(s) 1486, 1710
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1267, 1307, 1590
BcuI ACTAGT 1 cut(s) 1220
BfaI CTAG 7 cut(s) 63, 288, 644, 663, 1221, 1280, 1560
BfmI CTRYAG 1 cut(s) 1416
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1724
BisI GCNGC 5 cut(s) 216, 333, 410, 1214, 1298
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 5 cut(s) 217, 334, 411, 1215, 1299
Bme1390I CCNGG 6 cut(s) 15, 58, 473, 602, 709, 1585
Bme18I GGWCC 4 cut(s) 694, 729, 1013, 1441
BmgBI CACGTC 1 cut(s) 1600
BmgT120I GGNCC 5 cut(s) 694, 729, 875, 1013, 1441
BmiI GGNNCC 5 cut(s) 696, 750, 1015, 1539, 1763
BmrFI CCNGG 6 cut(s) 15, 58, 473, 602, 709, 1585
BmsI GCATC 4 cut(s) 408, 602, 656, 1230
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 2 cut(s) 448, 1632
BpmI CTGGAG 1 cut(s) 662
Bpu1102I GCTNAGC 1 cut(s) 834
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 1680
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 3 cut(s) 1335, 1611, 1729
BsaI GGTCTC 2 cut(s) 118, 303
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 714, 1141, 1462, 1560
Bse118I RCCGGY 2 cut(s) 829, 1134
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 2 cut(s) 650, 1316
Bse8I GATNNNNATC 3 cut(s) 1335, 1611, 1729
BseAI TCCGGA 1 cut(s) 852
BseBI CCWGG 5 cut(s) 58, 473, 602, 709, 1585
BseGI GGATG 3 cut(s) 937, 1336, 1750
BseJI GATNNNNATC 3 cut(s) 1335, 1611, 1729
BseLI CCNNNNNNNGG 5 cut(s) 176, 714, 1141, 1462, 1560
BseMI GCAATG 2 cut(s) 650, 1316
BseMII CTCAG 8 cut(s) 126, 216, 314, 647, 717, 825, 963, 1761
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 4 cut(s) 1073, 1094, 1115, 1136
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 5 cut(s) 227, 319, 396, 1225, 1284
BshFI GGCC 3 cut(s) 270, 877, 1138
BshNI GGYRCC 1 cut(s) 1537
BsiHKAI GWGCWC 3 cut(s) 154, 1580, 1684
BsiSI CCGG 4 cut(s) 14, 830, 853, 1135
BslFI GGGAC 2 cut(s) 193, 999
BslI CCNNNNNNNGG 5 cut(s) 176, 714, 1141, 1462, 1560
BsmAI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1267, 1307, 1590
BsmBI CGTCTC 2 cut(s) 680, 1590
BsmFI GGGAC 2 cut(s) 193, 999
BsmI GAATGC 1 cut(s) 1630
BsnI GGCC 3 cut(s) 270, 877, 1138
Bso31I GGTCTC 2 cut(s) 118, 303
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 154, 1580, 1684
Bsp13I TCCGGA 1 cut(s) 852
Bsp1720I GCTNAGC 1 cut(s) 834
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 1 cut(s) 724
BspANI GGCC 3 cut(s) 270, 877, 1138
BspCNI CTCAG 8 cut(s) 127, 215, 313, 646, 716, 826, 962, 1762
BspEI TCCGGA 1 cut(s) 852
BspLI GGNNCC 5 cut(s) 696, 750, 1015, 1539, 1763
BspMAI CTGCAG 1 cut(s) 1420
BspPI GGATC 8 cut(s) 32, 312, 680, 773, 1043, 1064, 1756, 1769
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1537
BspTNI GGTCTC 2 cut(s) 118, 303
BsrDI GCAATG 2 cut(s) 650, 1316
BsrFI RCCGGY 2 cut(s) 829, 1134
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 2 cut(s) 829, 1134
BssSI CACGAG 3 cut(s) 789, 1593, 1677
BssT1I CCWWGG 6 cut(s) 1017, 1053, 1074, 1095, 1116, 1359
Bst2BI CACGAG 3 cut(s) 789, 1593, 1677
Bst2UI CCWGG 5 cut(s) 58, 473, 602, 709, 1585
Bst4CI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
Bst6I CTCTTC 2 cut(s) 73, 121
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 373, 414
BstDEI CTNAG 8 cut(s) 135, 202, 300, 633, 703, 834, 949, 1770
BstDSI CCRYGG 1 cut(s) 1359
BstF5I GGATG 3 cut(s) 937, 1336, 1750
BstMAI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1267, 1307, 1590
BstMWI GCNNNNNNNGC 3 cut(s) 341, 350, 377
BstNI CCWGG 5 cut(s) 58, 473, 602, 709, 1585
BstNSI RCATGY 4 cut(s) 515, 570, 1250, 1354
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 6 cut(s) 13, 56, 471, 600, 707, 1583
BstSFI CTRYAG 1 cut(s) 1416
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 5 cut(s) 227, 319, 396, 1225, 1284
BstV2I GAAGAC 2 cut(s) 448, 1632
BstX2I RGATCY 3 cut(s) 304, 1724, 1761
BstYI RGATCY 3 cut(s) 304, 1724, 1761
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 3 cut(s) 270, 877, 1138
BtgI CCRYGG 1 cut(s) 1359
BtrI CACGTC 1 cut(s) 1600
BtsCI GGATG 3 cut(s) 937, 1336, 1750
BtsIMutI CAGTG 4 cut(s) 114, 154, 494, 864
Cac8I GCNNGC 2 cut(s) 373, 414
CaiI CAGNNNCTG 1 cut(s) 899
Cfr10I RCCGGY 2 cut(s) 829, 1134
Cfr13I GGNCC 5 cut(s) 694, 729, 875, 1013, 1441
Csp6I GTAC 1 cut(s) 1170
CspCI CAANNNNNGTGG 1 cut(s) 1754
CviAII CATG 7 cut(s) 512, 567, 738, 1247, 1351, 1360, 1534
CviQI GTAC 1 cut(s) 1170
DdeI CTNAG 8 cut(s) 135, 202, 300, 633, 703, 834, 949, 1770
DraIII CACNNNGTG 1 cut(s) 350
EaeI YGGCCR 2 cut(s) 268, 1136
Eam1104I CTCTTC 2 cut(s) 73, 121
EarI CTCTTC 2 cut(s) 73, 121
Ecl136II GAGCTC 1 cut(s) 1578
Eco130I CCWWGG 6 cut(s) 1017, 1053, 1074, 1095, 1116, 1359
Eco24I GRGCYC 1 cut(s) 1580
Eco31I GGTCTC 2 cut(s) 118, 303
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 4 cut(s) 694, 729, 1013, 1441
Eco53kI GAGCTC 1 cut(s) 1578
Eco57I CTGAAG 2 cut(s) 413, 876
EcoICRI GAGCTC 1 cut(s) 1578
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 5 cut(s) 56, 471, 600, 707, 1583
EcoRV GATATC 1 cut(s) 162
EcoT14I CCWWGG 6 cut(s) 1017, 1053, 1074, 1095, 1116, 1359
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 1580
ErhI CCWWGG 6 cut(s) 1017, 1053, 1074, 1095, 1116, 1359
Esp3I CGTCTC 2 cut(s) 680, 1590
FaeI CATG 7 cut(s) 515, 570, 741, 1250, 1354, 1363, 1537
FalI AAGNNNNNCTT 4 cut(s) 580, 612, 1453, 1485
FaqI GGGAC 2 cut(s) 193, 999
FatI CATG 7 cut(s) 511, 566, 737, 1246, 1350, 1359, 1533
FbaI TGATCA 2 cut(s) 1486, 1710
Fnu4HI GCNGC 5 cut(s) 216, 333, 410, 1214, 1298
FokI GGATG 3 cut(s) 924, 1323, 1757
FriOI GRGCYC 1 cut(s) 1580
Fsp4HI GCNGC 5 cut(s) 216, 333, 410, 1214, 1298
FspBI CTAG 7 cut(s) 63, 288, 644, 663, 1221, 1280, 1560
GluI GCNGC 5 cut(s) 216, 333, 410, 1214, 1298
GsuI CTGGAG 1 cut(s) 662
HaeIII GGCC 3 cut(s) 270, 877, 1138
HapII CCGG 4 cut(s) 14, 830, 853, 1135
Hin1II CATG 7 cut(s) 515, 570, 741, 1250, 1354, 1363, 1537
HincII GTYRAC 2 cut(s) 141, 1348
HindII GTYRAC 2 cut(s) 141, 1348
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 6 cut(s) 200, 499, 808, 1112, 1547, 1612
HpaI GTTAAC 1 cut(s) 141
HpaII CCGG 4 cut(s) 14, 830, 853, 1135
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1348, 1667
Hpy188III TCNNGA 8 cut(s) 28, 302, 689, 853, 956, 1199, 1280, 1493
Hpy8I GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1348, 1667
HpyAV CCTTC 4 cut(s) 388, 976, 1350, 1455
HpyCH4III ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
HpyCH4IV ACGT 3 cut(s) 195, 673, 1599
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 350, 377
HpyF3I CTNAG 8 cut(s) 135, 202, 300, 633, 703, 834, 949, 1770
HpySE526I ACGT 3 cut(s) 195, 673, 1599
Hsp92II CATG 7 cut(s) 515, 570, 741, 1250, 1354, 1363, 1537
Kpn2I TCCGGA 1 cut(s) 852
Ksp22I TGATCA 2 cut(s) 1486, 1710
KspAI GTTAAC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 1192, 1294
Lsp1109I GCAGC 5 cut(s) 227, 319, 396, 1225, 1284
LweI GCATC 4 cut(s) 408, 602, 656, 1230
MaeI CTAG 7 cut(s) 63, 288, 644, 663, 1221, 1280, 1560
MaeII ACGT 3 cut(s) 195, 673, 1599
MaeIII GTNAC 3 cut(s) 191, 886, 1529
MflI RGATCY 3 cut(s) 304, 1724, 1761
MhlI GDGCHC 3 cut(s) 154, 1580, 1684
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 5 cut(s) 31, 315, 1030, 1179, 1688
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 1 cut(s) 194
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 628
MroI TCCGGA 1 cut(s) 852
MroXI GAANNNNTTC 3 cut(s) 1006, 1386, 1465
MscI TGGCCA 1 cut(s) 270
MseI TTAA 4 cut(s) 140, 318, 1619, 1661
MslI CAYNNNNRTG 3 cut(s) 463, 558, 1221
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 218, 726
MspI CCGG 4 cut(s) 14, 830, 853, 1135
MspR9I CCNGG 6 cut(s) 15, 58, 473, 602, 709, 1585
Mva1269I GAATGC 1 cut(s) 1630
MvaI CCWGG 5 cut(s) 58, 473, 602, 709, 1585
MwoI GCNNNNNNNGC 3 cut(s) 341, 350, 377
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 1359
NlaIII CATG 7 cut(s) 515, 570, 741, 1250, 1354, 1363, 1537
NlaIV GGNNCC 5 cut(s) 696, 750, 1015, 1539, 1763
NmuCI GTSAC 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 4 cut(s) 515, 570, 1250, 1354
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 1221
PciI ACATGT 2 cut(s) 566, 1246
PctI GAATGC 1 cut(s) 1630
PdmI GAANNNNTTC 3 cut(s) 1006, 1386, 1465
PfeI GAWTC 5 cut(s) 499, 808, 1112, 1547, 1612
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 5 cut(s) 217, 334, 411, 1215, 1299
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 694
PscI ACATGT 2 cut(s) 566, 1246
PshAI GACNNNNGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 1580
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 5 cut(s) 56, 471, 600, 707, 1583
PspGI CCWGG 5 cut(s) 56, 471, 600, 707, 1583
PspN4I GGNNCC 5 cut(s) 696, 750, 1015, 1539, 1763
PspPI GGNCC 5 cut(s) 694, 729, 875, 1013, 1441
PspPPI RGGWCCY 1 cut(s) 694
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PstI CTGCAG 1 cut(s) 1420
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 3 cut(s) 304, 1724, 1761
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 1171
RsaNI GTAC 1 cut(s) 1170
RseI CAYNNNNRTG 3 cut(s) 463, 558, 1221
SacI GAGCTC 1 cut(s) 1580
SaqAI TTAA 4 cut(s) 140, 318, 1619, 1661
SatI GCNGC 5 cut(s) 216, 333, 410, 1214, 1298
Sau96I GGNCC 5 cut(s) 694, 729, 875, 1013, 1441
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 6 cut(s) 15, 58, 473, 602, 709, 1585
SduI GDGCHC 3 cut(s) 154, 1580, 1684
SfaNI GCATC 4 cut(s) 408, 602, 656, 1230
SfcI CTRYAG 1 cut(s) 1416
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 4 cut(s) 694, 729, 1013, 1441
SmiMI CAYNNNNRTG 3 cut(s) 463, 558, 1221
SmlI CTYRAG 1 cut(s) 1695
SmoI CTYRAG 1 cut(s) 1695
SpeI ACTAGT 1 cut(s) 1220
Sse9I AATT 5 cut(s) 31, 315, 1030, 1179, 1688
SsiI CCGC 1 cut(s) 724
SspMI CTAG 7 cut(s) 63, 288, 644, 663, 1221, 1280, 1560
SstI GAGCTC 1 cut(s) 1580
StyD4I CCNGG 6 cut(s) 13, 56, 471, 600, 707, 1583
StyI CCWWGG 6 cut(s) 1017, 1053, 1074, 1095, 1116, 1359
TaaI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
TaiI ACGT 3 cut(s) 198, 676, 1602
TaqI TCGA 3 cut(s) 385, 573, 617
TasI AATT 5 cut(s) 31, 315, 1030, 1179, 1688
TatI WGTACW 1 cut(s) 1169
TfiI GAWTC 5 cut(s) 499, 808, 1112, 1547, 1612
Tru1I TTAA 4 cut(s) 140, 318, 1619, 1661
Tru9I TTAA 4 cut(s) 140, 318, 1619, 1661
TscAI CASTG 4 cut(s) 114, 154, 501, 871
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 5 cut(s) 215, 332, 409, 1213, 1297
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 4 cut(s) 240, 299, 959, 1395
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 4 cut(s) 114, 154, 501, 871
Van91I CCANNNNNTGG 1 cut(s) 176
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 4 cut(s) 694, 729, 1013, 1441
XapI RAATTY 1 cut(s) 31
XbaI TCTAGA 1 cut(s) 1279
XceI RCATGY 4 cut(s) 515, 570, 1250, 1354
XmnI GAANNNNTTC 3 cut(s) 1006, 1386, 1465
XspI CTAG 7 cut(s) 63, 288, 644, 663, 1221, 1280, 1560
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.