RchiOBHm_Chr4g0440321

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005350
Physical Location & Seq
Reverse (-)
62491125 .. 62495998
4874 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40829

Sequence Viewer

Length: 1743 bp
ATGGGAAAAACGCCGGGAAAATGGATCAAGAATTTGCTTCTTGGGAAGAAATCGTCGAGGTCTAGTTTGTCCAAAGGAAGAGAGAAGTCTGCAACTAAAGGGGAGACAGTGGTCTCTTCAAAGTTGTCTGTGCCTGAGTTAACTGTCAGTGCACCGGTGATATCACCACCATTCGTTGGGACGGTTGTTAGTAACGTGGACTCAGAAATCAGAGCAGCTGTGGAAATACCAAACGATAACATTACACTTTCATCGGCAAAGGAAGATGGCCAAACACAAGCTATCTCTAGTTCGGGGTCTCAGGATCTTCAGAGAATTAACCGAGAGGAAGCTGCCACAAAGGCACAGAGTGCTTATAGAGGATATGTGGCTCGCCGTGCATATCGAACCCTGAAGGGCATCATAAGGCTGCAAGCACTTATTCGTGGTCATTTGGTTAGAAGACAAGCTGTATCCACATTATTATGTGTCCAGGGGATTGTGAAGTTTCAAGCACTGATTCGTGGTCAGCATGTTAGACATTCTGATATTGGGGTTGAAGTGCATAAAACACATCAGGGTGCTACATGTTTCGATTACTCTGGTGTGAGTGCATCTTCCCAGGTGGACAGGCTTTCGAAAAATGCATTTGCTCAGAAGCTTCTGGCATCATTGCCTACTGCTAGTCCTCTACGTCTCCAGTATGATCCAGAGGACCCGAACTCGGCCTGGGTGTGGCTTGACCGCTGGACCAGATCATGCTTTTGGGAACCTGTTTTACAACTAAAGAAAAAACCTGATCCGAAGTCTCGTGGCAAATACAAGAAGATTCAAACAGTAGAAACTGAACCAGCTAAGCCAAAAAGAACTGTCCGGAGACTGTCCACTGAAAATGGGCCAAATCGTGTCACTTCAGATACTGAAAAGCCTAAACGAAATATGAGGAAAGTTTCAAGTCATCCAATGAACTCAGTTCAGGAACATCAACAAAACGGAGTTGAGAAGGTCAAAAGTAATGTCAGAAAAGTTTCTGGTCCCAAGGAGGTTTCCAATTCCAAAAAGGTTTCTGATACCAAGGAGGAGGTTTCTGATTCCAAGGAGGAGGTTTCTGATCAGCCAGAGGTTGATAAGGAGATACCAAAGCACAGTACAGAAACAATTTTAGCTCCTGCTGTTCTTGATGATTCAGAGCAGCACACTAGTGAGATTTCTCCGAAGATACAACATGTGGAAGTTTCTGTCTCCAAACACTCTGATCTAGAAGAATGTATGGAGCTGCCACCAACAGATGAGACATTGCAAAAGTTAGATGACCATCCTTCTGTTGACATGCAATCCATGGAGAAGAATGGCAAAAATGAAGAAGTTCAAGCACTAAATGAGGTTCTAACCTCTGCAGACAACTTTATTAGCAACGAGGACCAGAAAACCAGCCAGAGAAGGGCTTCTTTACCTGTGAAGTTTGATCATCGGGATAATGGGGTTCAAACCACACCAAGAGTGCCAAGTTACATGGCACCAACTGAATCTGCCAGAGCTAGGCTGAAAGGACAGAGCTCCCCCAGGTTTGCACGAGACGTGATTGATAAGAATCTTTTAACCAGACGGCATTCTCTGTCTTCTTCCACCAACACCAAGTTAAGTTTACTTTCTCCACGAGCACAAAAATTGGCTCAAGGCAAAGGAGTGATCAGAACTGACAGGTCTTTATCATCTTCAAGGGATGGTTTTGATAAGGGGATCCAACCTGAGTGGAGGAGGTGA

Protein Analysis

580

Amino Acids

64.52

Weight (kDa)

9.67

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 483 - 544 2.2e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1495
AccB7I CCANNNNNTGG 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 852
AciI CCGC 1 cut(s) 724
AclWI GGATC 6 cut(s) 32, 312, 680, 773, 1714, 1727
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 3 cut(s) 293, 413, 876
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 1 cut(s) 1129
AfiI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1420, 1518
AflIII ACRYGT 2 cut(s) 566, 1204
AgeI ACCGGT 1 cut(s) 154
AgsI TTSAA 8 cut(s) 120, 491, 539, 812, 933, 1349, 1466, 1698
AhlI ACTAGT 1 cut(s) 1178
AjiI CACGTC 1 cut(s) 1558
AjnI CCWGG 4 cut(s) 471, 600, 707, 1541
AleI CACNNNNGTG 1 cut(s) 1179
Alw21I GWGCWC 3 cut(s) 154, 1538, 1642
Alw26I GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1225, 1265, 1548
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 6 cut(s) 32, 312, 680, 773, 1714, 1727
AlwNI CAGNNNCTG 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 852
AoxI GGCC 3 cut(s) 268, 705, 875
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 5 cut(s) 215, 332, 409, 1171, 1255
ApoI RAATTY 1 cut(s) 31
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
AsiGI ACCGGT 1 cut(s) 154
Asp700I GAANNNNTTC 3 cut(s) 1006, 1344, 1423
AspS9I GGNCC 5 cut(s) 694, 729, 875, 1013, 1399
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 2 cut(s) 156, 169
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 4 cut(s) 694, 729, 1013, 1399
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 1719
BanI GGYRCC 1 cut(s) 1495
BanII GRGCYC 1 cut(s) 1538
BauI CACGAG 3 cut(s) 789, 1551, 1635
BbsI GAAGAC 2 cut(s) 448, 1590
Bbv12I GWGCWC 3 cut(s) 154, 1538, 1642
BbvI GCAGC 5 cut(s) 227, 319, 396, 1183, 1242
BccI CCATC 3 cut(s) 260, 1302, 1697
BceAI ACGGC 2 cut(s) 360, 1601
BciT130I CCWGG 4 cut(s) 473, 602, 709, 1543
BciVI GTATCC 1 cut(s) 463
BclI TGATCA 3 cut(s) 1090, 1444, 1668
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1225, 1265, 1548
BcuI ACTAGT 1 cut(s) 1178
BfaI CTAG 6 cut(s) 63, 288, 663, 1179, 1238, 1518
BfmI CTRYAG 1 cut(s) 1374
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 341
BisI GCNGC 5 cut(s) 216, 333, 410, 1172, 1256
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 5 cut(s) 217, 334, 411, 1173, 1257
Bme1390I CCNGG 5 cut(s) 15, 473, 602, 709, 1543
Bme18I GGWCC 4 cut(s) 694, 729, 1013, 1399
BmgBI CACGTC 1 cut(s) 1558
BmgT120I GGNCC 5 cut(s) 694, 729, 875, 1013, 1399
BmiI GGNNCC 5 cut(s) 696, 750, 1015, 1497, 1721
BmrFI CCNGG 5 cut(s) 15, 473, 602, 709, 1543
BmsI GCATC 3 cut(s) 408, 602, 656
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 2 cut(s) 448, 1590
BpmI CTGGAG 1 cut(s) 662
Bpu1102I GCTNAGC 1 cut(s) 834
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 1638
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 2 cut(s) 1293, 1569
BsaI GGTCTC 2 cut(s) 118, 303
BsaJI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1053, 1074, 1317, 1541
BsaWI WCCGGW 2 cut(s) 154, 852
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1420, 1518
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 2 cut(s) 650, 1274
Bse8I GATNNNNATC 2 cut(s) 1293, 1569
BseAI TCCGGA 1 cut(s) 852
BseBI CCWGG 4 cut(s) 473, 602, 709, 1543
BseDI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1053, 1074, 1317, 1541
BseGI GGATG 3 cut(s) 937, 1294, 1708
BseJI GATNNNNATC 2 cut(s) 1293, 1569
BseLI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1420, 1518
BseMI GCAATG 2 cut(s) 650, 1274
BseMII CTCAG 6 cut(s) 126, 216, 314, 647, 963, 1719
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 2 cut(s) 1073, 1094
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 5 cut(s) 227, 319, 396, 1183, 1242
BshFI GGCC 3 cut(s) 270, 707, 877
BshNI GGYRCC 1 cut(s) 1495
BshTI ACCGGT 1 cut(s) 154
BsiHKAI GWGCWC 3 cut(s) 154, 1538, 1642
BsiSI CCGG 3 cut(s) 14, 155, 853
BslFI GGGAC 2 cut(s) 193, 999
BslI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1420, 1518
BsmAI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1225, 1265, 1548
BsmBI CGTCTC 2 cut(s) 680, 1548
BsmFI GGGAC 2 cut(s) 193, 999
BsmI GAATGC 1 cut(s) 1588
BsnI GGCC 3 cut(s) 270, 707, 877
Bso31I GGTCTC 2 cut(s) 118, 303
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 154, 1538, 1642
Bsp13I TCCGGA 1 cut(s) 852
Bsp1720I GCTNAGC 1 cut(s) 834
Bsp19I CCATGG 1 cut(s) 1317
BspACI CCGC 1 cut(s) 724
BspANI GGCC 3 cut(s) 270, 707, 877
BspCNI CTCAG 6 cut(s) 127, 215, 313, 646, 962, 1720
BspEI TCCGGA 1 cut(s) 852
BspLI GGNNCC 5 cut(s) 696, 750, 1015, 1497, 1721
BspMAI CTGCAG 1 cut(s) 1378
BspPI GGATC 6 cut(s) 32, 312, 680, 773, 1714, 1727
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1495
BspTNI GGTCTC 2 cut(s) 118, 303
BsrDI GCAATG 2 cut(s) 650, 1274
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1053, 1074, 1317, 1541
BssSI CACGAG 3 cut(s) 789, 1551, 1635
BssT1I CCWWGG 4 cut(s) 1017, 1053, 1074, 1317
Bst2BI CACGAG 3 cut(s) 789, 1551, 1635
Bst2UI CCWGG 4 cut(s) 473, 602, 709, 1543
Bst4CI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1127
Bst6I CTCTTC 2 cut(s) 73, 121
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 373, 414
BstDEI CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1728
BstDSI CCRYGG 1 cut(s) 1317
BstF5I GGATG 3 cut(s) 937, 1294, 1708
BstMAI GTCTC 9 cut(s) 98, 118, 303, 680, 792, 850, 1225, 1265, 1548
BstMWI GCNNNNNNNGC 3 cut(s) 341, 350, 377
BstNI CCWGG 4 cut(s) 473, 602, 709, 1543
BstNSI RCATGY 4 cut(s) 515, 570, 1208, 1312
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 5 cut(s) 13, 471, 600, 707, 1541
BstSFI CTRYAG 1 cut(s) 1374
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 5 cut(s) 227, 319, 396, 1183, 1242
BstV2I GAAGAC 2 cut(s) 448, 1590
BstX2I RGATCY 2 cut(s) 304, 1719
BstYI RGATCY 2 cut(s) 304, 1719
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 3 cut(s) 270, 707, 877
BtgI CCRYGG 1 cut(s) 1317
BtrI CACGTC 1 cut(s) 1558
BtsCI GGATG 3 cut(s) 937, 1294, 1708
BtsIMutI CAGTG 4 cut(s) 114, 154, 494, 864
Cac8I GCNNGC 2 cut(s) 373, 414
CaiI CAGNNNCTG 1 cut(s) 899
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 5 cut(s) 694, 729, 875, 1013, 1399
Csp6I GTAC 1 cut(s) 1128
CspAI ACCGGT 1 cut(s) 154
CspCI CAANNNNNGTGG 1 cut(s) 1712
CviAII CATG 7 cut(s) 512, 567, 738, 1205, 1309, 1318, 1492
CviQI GTAC 1 cut(s) 1128
DdeI CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1728
DraIII CACNNNGTG 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 73, 121
EarI CTCTTC 2 cut(s) 73, 121
Ecl136II GAGCTC 1 cut(s) 1536
Eco130I CCWWGG 4 cut(s) 1017, 1053, 1074, 1317
Eco24I GRGCYC 1 cut(s) 1538
Eco31I GGTCTC 2 cut(s) 118, 303
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 4 cut(s) 694, 729, 1013, 1399
Eco53kI GAGCTC 1 cut(s) 1536
Eco57I CTGAAG 3 cut(s) 293, 413, 876
EcoICRI GAGCTC 1 cut(s) 1536
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 4 cut(s) 471, 600, 707, 1541
EcoRV GATATC 1 cut(s) 162
EcoT14I CCWWGG 4 cut(s) 1017, 1053, 1074, 1317
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 1538
ErhI CCWWGG 4 cut(s) 1017, 1053, 1074, 1317
Esp3I CGTCTC 2 cut(s) 680, 1548
FaeI CATG 7 cut(s) 515, 570, 741, 1208, 1312, 1321, 1495
FalI AAGNNNNNCTT 2 cut(s) 1411, 1443
FaqI GGGAC 2 cut(s) 193, 999
FatI CATG 7 cut(s) 511, 566, 737, 1204, 1308, 1317, 1491
FbaI TGATCA 3 cut(s) 1090, 1444, 1668
Fnu4HI GCNGC 5 cut(s) 216, 333, 410, 1172, 1256
FokI GGATG 3 cut(s) 924, 1281, 1715
FriOI GRGCYC 1 cut(s) 1538
Fsp4HI GCNGC 5 cut(s) 216, 333, 410, 1172, 1256
FspBI CTAG 6 cut(s) 63, 288, 663, 1179, 1238, 1518
GluI GCNGC 5 cut(s) 216, 333, 410, 1172, 1256
GsuI CTGGAG 1 cut(s) 662
HaeIII GGCC 3 cut(s) 270, 707, 877
HapII CCGG 3 cut(s) 14, 155, 853
Hin1II CATG 7 cut(s) 515, 570, 741, 1208, 1312, 1321, 1495
HincII GTYRAC 2 cut(s) 141, 1306
HindII GTYRAC 2 cut(s) 141, 1306
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 7 cut(s) 200, 499, 808, 1070, 1163, 1505, 1570
HpaI GTTAAC 1 cut(s) 141
HpaII CCGG 3 cut(s) 14, 155, 853
HphI GGTGA 2 cut(s) 156, 169
Hpy166II GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1306, 1625
Hpy188III TCNNGA 8 cut(s) 28, 302, 689, 853, 956, 1157, 1238, 1451
Hpy8I GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1306, 1625
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 4 cut(s) 388, 976, 1308, 1413
HpyCH4III ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1127
HpyCH4IV ACGT 3 cut(s) 195, 673, 1557
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 350, 377
HpyF3I CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1728
HpySE526I ACGT 3 cut(s) 195, 673, 1557
Hsp92II CATG 7 cut(s) 515, 570, 741, 1208, 1312, 1321, 1495
Kpn2I TCCGGA 1 cut(s) 852
Ksp22I TGATCA 3 cut(s) 1090, 1444, 1668
KspAI GTTAAC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 1150, 1252, 1541
Lsp1109I GCAGC 5 cut(s) 227, 319, 396, 1183, 1242
LweI GCATC 3 cut(s) 408, 602, 656
MaeI CTAG 6 cut(s) 63, 288, 663, 1179, 1238, 1518
MaeII ACGT 3 cut(s) 195, 673, 1557
MaeIII GTNAC 3 cut(s) 191, 886, 1487
MflI RGATCY 2 cut(s) 304, 1719
MhlI GDGCHC 3 cut(s) 154, 1538, 1642
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 5 cut(s) 31, 315, 1030, 1137, 1646
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 1 cut(s) 194
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 628
MroI TCCGGA 1 cut(s) 852
MroXI GAANNNNTTC 3 cut(s) 1006, 1344, 1423
MscI TGGCCA 1 cut(s) 270
MseI TTAA 4 cut(s) 140, 318, 1577, 1619
MslI CAYNNNNRTG 3 cut(s) 463, 558, 1179
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 218, 726
MspI CCGG 3 cut(s) 14, 155, 853
MspR9I CCNGG 5 cut(s) 15, 473, 602, 709, 1543
Mva1269I GAATGC 1 cut(s) 1588
MvaI CCWGG 4 cut(s) 473, 602, 709, 1543
MwoI GCNNNNNNNGC 3 cut(s) 341, 350, 377
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 1317
NlaIII CATG 7 cut(s) 515, 570, 741, 1208, 1312, 1321, 1495
NlaIV GGNNCC 5 cut(s) 696, 750, 1015, 1497, 1721
NmeAIII GCCGAG 1 cut(s) 683
NmuCI GTSAC 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 4 cut(s) 515, 570, 1208, 1312
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 1179
PciI ACATGT 2 cut(s) 566, 1204
PctI GAATGC 1 cut(s) 1588
PdmI GAANNNNTTC 3 cut(s) 1006, 1344, 1423
PfeI GAWTC 6 cut(s) 499, 808, 1070, 1163, 1505, 1570
PflMI CCANNNNNTGG 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 154
PkrI GCNGC 5 cut(s) 217, 334, 411, 1173, 1257
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 694
PscI ACATGT 2 cut(s) 566, 1204
PshAI GACNNNNGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 1538
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 4 cut(s) 471, 600, 707, 1541
PspGI CCWGG 4 cut(s) 471, 600, 707, 1541
PspN4I GGNNCC 5 cut(s) 696, 750, 1015, 1497, 1721
PspPI GGNCC 5 cut(s) 694, 729, 875, 1013, 1399
PspPPI RGGWCCY 1 cut(s) 694
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PstI CTGCAG 1 cut(s) 1378
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 2 cut(s) 304, 1719
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 1129
RsaNI GTAC 1 cut(s) 1128
RseI CAYNNNNRTG 3 cut(s) 463, 558, 1179
SacI GAGCTC 1 cut(s) 1538
SaqAI TTAA 4 cut(s) 140, 318, 1577, 1619
SatI GCNGC 5 cut(s) 216, 333, 410, 1172, 1256
Sau96I GGNCC 5 cut(s) 694, 729, 875, 1013, 1399
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 5 cut(s) 15, 473, 602, 709, 1543
SduI GDGCHC 3 cut(s) 154, 1538, 1642
SfaNI GCATC 3 cut(s) 408, 602, 656
SfcI CTRYAG 1 cut(s) 1374
SfuI TTCGAA 1 cut(s) 617
SgrAI CRCCGGYG 1 cut(s) 154
SinI GGWCC 4 cut(s) 694, 729, 1013, 1399
SmiMI CAYNNNNRTG 3 cut(s) 463, 558, 1179
SmlI CTYRAG 1 cut(s) 1653
SmoI CTYRAG 1 cut(s) 1653
SpeI ACTAGT 1 cut(s) 1178
Sse9I AATT 5 cut(s) 31, 315, 1030, 1137, 1646
SsiI CCGC 1 cut(s) 724
SspMI CTAG 6 cut(s) 63, 288, 663, 1179, 1238, 1518
SstI GAGCTC 1 cut(s) 1538
StyD4I CCNGG 5 cut(s) 13, 471, 600, 707, 1541
StyI CCWWGG 4 cut(s) 1017, 1053, 1074, 1317
TaaI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1127
TaiI ACGT 3 cut(s) 198, 676, 1560
TaqI TCGA 4 cut(s) 56, 385, 573, 617
TasI AATT 5 cut(s) 31, 315, 1030, 1137, 1646
TatI WGTACW 1 cut(s) 1127
TfiI GAWTC 6 cut(s) 499, 808, 1070, 1163, 1505, 1570
Tru1I TTAA 4 cut(s) 140, 318, 1577, 1619
Tru9I TTAA 4 cut(s) 140, 318, 1577, 1619
TscAI CASTG 4 cut(s) 114, 154, 501, 871
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 5 cut(s) 215, 332, 409, 1171, 1255
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 3 cut(s) 240, 959, 1353
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 4 cut(s) 114, 154, 501, 871
Van91I CCANNNNNTGG 1 cut(s) 176
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 4 cut(s) 694, 729, 1013, 1399
XapI RAATTY 1 cut(s) 31
XbaI TCTAGA 1 cut(s) 1237
XceI RCATGY 4 cut(s) 515, 570, 1208, 1312
XmnI GAANNNNTTC 3 cut(s) 1006, 1344, 1423
XspI CTAG 6 cut(s) 63, 288, 663, 1179, 1238, 1518
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.