Rw4G032230

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005350
Physical Location & Seq
Reverse (-)
59382839 .. 59386800
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032230.1

Sequence Viewer

Length: 1788 bp
ATGGGAAAAACGCCGGGAAAATGGATCAAGAATTTGCTTCTTGGGAAGAAATCGTCGAGGTCTAGTTTGTCCAAAGGAAGAGAGAAGTCTGCAACTAAAGGGGAGACAGTGGTCTCTTCAAAGTTGTCTGTGCCTGAGTTAACTGTCAGTGCACCTGTGATATCACCACCATTGGTTGGGACGGTTGTTAGTAACGTGGACTCAGAAATCAGAGCAGCTGTGGAAATACCAAACGATAACATTGCACTTTCATCGGCAAAGGAAGATGGCCAAACACAAGCTATCTCTAGTTCGGGGTCTCAGGATCTTCAGAGAATTAACCGAGAGGAAGCTGCCACAAAGGCACAGAGTGCTTATAGAGGATATGTGGCTCGCCGTGCGTATCGAACCCTGAAGGGCATCATAAGGCTGCAAGCACTTATTCGTGGTCATTTGGTTAGAAGACAAGCTGTATCCACATTATTATGTGTCCAGGGGATTGTGAAGTTTCAAGCACTGATTCGTGGTCAGCATGTTAGACATTCTGATATTGGGGTTGAAGTGCATAAAACACATCAGGGTGCTACATGTTTCGATTACTCTGGTGTGAGTGCATCTTCCCAGGTGGACAGGCTTTCGAAAAATGCATTTGCTCAGAAGCTTCTGGCATCATTGCCTACTGCTAGTCCTCTACGTCTCCAGTATAATCCAGAGGACCCGAACTCGGCCTGGGTGTGGCTTGACCGCTGGACCAGATCATGCTTTTGGGAACCTGTTTTACAACTAGAGAAAAAACCTGATCCGAAGTCTCGTGGCAAATACAAGAAGATTCAAACAGTAGAAACTGAACCAGCTAAGCCAAAAAGAACTGTCCGGAGACTGTCCACTGAAAATGGGCCAAATCGTGTCACTTCAGATACTGAAAAGCCTAAACGAAATATGAGGAAAGTTTCAAGTCATCCAATGAACTCAGTTCAGGAACATCAACAAAACGGAGTTGAGAAGGTCAAAAGTAATGTCAGAAAAGTTTCTGGTCCCAAGGAGGTTTCCAATTCCAAAAAGGTTTCTGATCCCAAGAATAAGGTTTCTGATCCCAAGGAGGAGGTTTCTGATACCAAGAAGGAGGTTTCTGATTCCAAGGAGGAGGTTTCTGATCGGCCAGAGGTTGATAAGGAGATACCAAAGCACAGTACAGAAACAATTTTAGCTCCTGCTGTTCTTGATGATTCAGAGCAGCAGCACACTAGTGAGATTTCTCTGGAGATACAACATGTGGAAGTTTCTATCTCCAAACACTCTGATCTAGAAGAATGTATGGAGCTGCCACCAACAGATGAGACATTGCAAAAGTTAGATGACCATCCTTCTGTTGACATGCAATCCATGGAGAAGAATGGCAAAAATGAAGAAGTTCAAGCACTAAATGAGGTTCTAACCTCTGCAGACAACTTTATTAGCAACGAGGACCAGAAAACCAGCCAGAGAAGGGCTTCTTTACCTGTGAAGTTTGATCATCGGGATAATGGGGTTCAAACCACACCAAGAGTGCCAAGTTACATGGCACCAACTGAATCTGCCAGAGCTAGGCTGAAAGGACAGAGCTCCCCCAGGTTTGCCCGAGACGTGATTGATAAGAATCTTTTAACCAGACGGCATTCTCTGTCTTCTTCCACCAACACCAAGTTAAGTTTACTTTCTCCACGAGCACAAAAATTGGCTCAAGGCAAAGGAGTGATCAGAACTGACAGATCTTTATCATCTTCAAGGGATGGTTTTGATAAGGGGATCCAACCTGAGTGGAGGAGGTGA

Protein Analysis

595

Amino Acids

66.19

Weight (kDa)

9.62

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 498 - 559 2.3e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1540
AccB7I CCANNNNNTGG 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 852
AciI CCGC 1 cut(s) 724
AclWI GGATC 7 cut(s) 32, 312, 773, 1043, 1064, 1759, 1772
AcoI YGGCCR 2 cut(s) 268, 1136
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 3 cut(s) 293, 413, 876
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 1 cut(s) 1171
AfiI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1465, 1563
AflIII ACRYGT 2 cut(s) 566, 1249
AgsI TTSAA 8 cut(s) 120, 491, 539, 812, 933, 1394, 1511, 1743
AhlI ACTAGT 1 cut(s) 1223
AjiI CACGTC 1 cut(s) 1603
AjnI CCWGG 4 cut(s) 471, 600, 707, 1586
AleI CACNNNNGTG 1 cut(s) 1224
Alw21I GWGCWC 3 cut(s) 154, 1583, 1687
Alw26I GTCTC 8 cut(s) 98, 118, 303, 680, 792, 850, 1310, 1593
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 7 cut(s) 32, 312, 773, 1043, 1064, 1759, 1772
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 1596
Aor13HI TCCGGA 1 cut(s) 852
AoxI GGCC 4 cut(s) 268, 705, 875, 1136
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 6 cut(s) 215, 332, 409, 1213, 1216, 1300
ApoI RAATTY 1 cut(s) 31
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
Asp700I GAANNNNTTC 3 cut(s) 1006, 1389, 1468
AspS9I GGNCC 5 cut(s) 694, 729, 875, 1013, 1444
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 156
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 1596
AvaII GGWCC 4 cut(s) 694, 729, 1013, 1444
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 1764
BanI GGYRCC 1 cut(s) 1540
BanII GRGCYC 1 cut(s) 1583
BauI CACGAG 2 cut(s) 789, 1680
BbsI GAAGAC 2 cut(s) 448, 1635
Bbv12I GWGCWC 3 cut(s) 154, 1583, 1687
BbvI GCAGC 6 cut(s) 227, 319, 396, 1225, 1228, 1287
BccI CCATC 3 cut(s) 260, 1347, 1742
BceAI ACGGC 2 cut(s) 360, 1646
BcgI CGANNNNNNTGC 4 cut(s) 224, 234, 258, 268
BciT130I CCWGG 4 cut(s) 473, 602, 709, 1588
BciVI GTATCC 1 cut(s) 463
BclI TGATCA 2 cut(s) 1489, 1713
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 8 cut(s) 98, 118, 303, 680, 792, 850, 1310, 1593
BcuI ACTAGT 1 cut(s) 1223
BfaI CTAG 7 cut(s) 63, 288, 663, 764, 1224, 1283, 1563
BfmI CTRYAG 1 cut(s) 1419
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1727
BisI GCNGC 6 cut(s) 216, 333, 410, 1214, 1217, 1301
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 6 cut(s) 217, 334, 411, 1215, 1218, 1302
Bme1390I CCNGG 5 cut(s) 15, 473, 602, 709, 1588
Bme18I GGWCC 4 cut(s) 694, 729, 1013, 1444
BmeT110I CYCGRG 1 cut(s) 1596
BmgBI CACGTC 1 cut(s) 1603
BmgT120I GGNCC 5 cut(s) 694, 729, 875, 1013, 1444
BmiI GGNNCC 5 cut(s) 696, 750, 1015, 1542, 1766
BmrFI CCNGG 5 cut(s) 15, 473, 602, 709, 1588
BmsI GCATC 3 cut(s) 408, 602, 656
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 2 cut(s) 448, 1635
BpmI CTGGAG 2 cut(s) 662, 1259
Bpu1102I GCTNAGC 1 cut(s) 834
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 1683
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 3 cut(s) 1338, 1614, 1732
BsaI GGTCTC 2 cut(s) 118, 303
BsaJI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1074, 1116, 1362, 1586
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1465, 1563
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 3 cut(s) 240, 650, 1319
Bse8I GATNNNNATC 3 cut(s) 1338, 1614, 1732
BseAI TCCGGA 1 cut(s) 852
BseBI CCWGG 4 cut(s) 473, 602, 709, 1588
BseDI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1074, 1116, 1362, 1586
BseGI GGATG 3 cut(s) 937, 1339, 1753
BseJI GATNNNNATC 3 cut(s) 1338, 1614, 1732
BseLI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1465, 1563
BseMI GCAATG 3 cut(s) 240, 650, 1319
BseMII CTCAG 6 cut(s) 126, 216, 314, 647, 963, 1764
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 2 cut(s) 1094, 1136
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 6 cut(s) 227, 319, 396, 1225, 1228, 1287
BshFI GGCC 4 cut(s) 270, 707, 877, 1138
BshNI GGYRCC 1 cut(s) 1540
BsiHKAI GWGCWC 3 cut(s) 154, 1583, 1687
BsiHKCI CYCGRG 1 cut(s) 1596
BsiSI CCGG 2 cut(s) 14, 853
BslFI GGGAC 2 cut(s) 193, 999
BslI CCNNNNNNNGG 5 cut(s) 176, 703, 714, 1465, 1563
BsmAI GTCTC 8 cut(s) 98, 118, 303, 680, 792, 850, 1310, 1593
BsmBI CGTCTC 2 cut(s) 680, 1593
BsmFI GGGAC 2 cut(s) 193, 999
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 4 cut(s) 270, 707, 877, 1138
Bso31I GGTCTC 2 cut(s) 118, 303
BsoBI CYCGRG 1 cut(s) 1596
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 154, 1583, 1687
Bsp13I TCCGGA 1 cut(s) 852
Bsp1720I GCTNAGC 1 cut(s) 834
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 1 cut(s) 724
BspANI GGCC 4 cut(s) 270, 707, 877, 1138
BspCNI CTCAG 6 cut(s) 127, 215, 313, 646, 962, 1765
BspEI TCCGGA 1 cut(s) 852
BspLI GGNNCC 5 cut(s) 696, 750, 1015, 1542, 1766
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 7 cut(s) 32, 312, 773, 1043, 1064, 1759, 1772
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1540
BspTNI GGTCTC 2 cut(s) 118, 303
BsrDI GCAATG 3 cut(s) 240, 650, 1319
BsrI ACTGG 1 cut(s) 679
BssECI CCNNGG 8 cut(s) 472, 600, 708, 1017, 1074, 1116, 1362, 1586
BssSI CACGAG 2 cut(s) 789, 1680
BssT1I CCWWGG 4 cut(s) 1017, 1074, 1116, 1362
Bst2BI CACGAG 2 cut(s) 789, 1680
Bst2UI CCWGG 4 cut(s) 473, 602, 709, 1588
Bst4CI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
Bst6I CTCTTC 2 cut(s) 73, 121
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 373, 414
BstDEI CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1773
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 3 cut(s) 937, 1339, 1753
BstMAI GTCTC 8 cut(s) 98, 118, 303, 680, 792, 850, 1310, 1593
BstMWI GCNNNNNNNGC 3 cut(s) 341, 350, 377
BstNI CCWGG 4 cut(s) 473, 602, 709, 1588
BstNSI RCATGY 4 cut(s) 515, 570, 1253, 1357
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 5 cut(s) 13, 471, 600, 707, 1586
BstSFI CTRYAG 1 cut(s) 1419
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 6 cut(s) 227, 319, 396, 1225, 1228, 1287
BstV2I GAAGAC 2 cut(s) 448, 1635
BstX2I RGATCY 3 cut(s) 304, 1727, 1764
BstYI RGATCY 3 cut(s) 304, 1727, 1764
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 4 cut(s) 270, 707, 877, 1138
BtgI CCRYGG 1 cut(s) 1362
BtrI CACGTC 1 cut(s) 1603
BtsCI GGATG 3 cut(s) 937, 1339, 1753
BtsIMutI CAGTG 4 cut(s) 114, 154, 494, 864
Cac8I GCNNGC 2 cut(s) 373, 414
CaiI CAGNNNCTG 1 cut(s) 899
Cfr13I GGNCC 5 cut(s) 694, 729, 875, 1013, 1444
Csp6I GTAC 1 cut(s) 1170
CspCI CAANNNNNGTGG 1 cut(s) 1757
CviAII CATG 7 cut(s) 512, 567, 738, 1250, 1354, 1363, 1537
CviQI GTAC 1 cut(s) 1170
DdeI CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1773
DraIII CACNNNGTG 1 cut(s) 350
EaeI YGGCCR 2 cut(s) 268, 1136
Eam1104I CTCTTC 2 cut(s) 73, 121
EarI CTCTTC 2 cut(s) 73, 121
Ecl136II GAGCTC 1 cut(s) 1581
Eco130I CCWWGG 4 cut(s) 1017, 1074, 1116, 1362
Eco24I GRGCYC 1 cut(s) 1583
Eco31I GGTCTC 2 cut(s) 118, 303
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 4 cut(s) 694, 729, 1013, 1444
Eco53kI GAGCTC 1 cut(s) 1581
Eco57I CTGAAG 3 cut(s) 293, 413, 876
Eco88I CYCGRG 1 cut(s) 1596
EcoICRI GAGCTC 1 cut(s) 1581
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 4 cut(s) 471, 600, 707, 1586
EcoRV GATATC 1 cut(s) 162
EcoT14I CCWWGG 4 cut(s) 1017, 1074, 1116, 1362
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 1583
ErhI CCWWGG 4 cut(s) 1017, 1074, 1116, 1362
Esp3I CGTCTC 2 cut(s) 680, 1593
FaeI CATG 7 cut(s) 515, 570, 741, 1253, 1357, 1366, 1540
FalI AAGNNNNNCTT 2 cut(s) 1456, 1488
FaqI GGGAC 2 cut(s) 193, 999
FatI CATG 7 cut(s) 511, 566, 737, 1249, 1353, 1362, 1536
FbaI TGATCA 2 cut(s) 1489, 1713
Fnu4HI GCNGC 6 cut(s) 216, 333, 410, 1214, 1217, 1301
FokI GGATG 3 cut(s) 924, 1326, 1760
FriOI GRGCYC 1 cut(s) 1583
Fsp4HI GCNGC 6 cut(s) 216, 333, 410, 1214, 1217, 1301
FspBI CTAG 7 cut(s) 63, 288, 663, 764, 1224, 1283, 1563
GluI GCNGC 6 cut(s) 216, 333, 410, 1214, 1217, 1301
GsuI CTGGAG 2 cut(s) 662, 1259
HaeIII GGCC 4 cut(s) 270, 707, 877, 1138
HapII CCGG 2 cut(s) 14, 853
Hin1II CATG 7 cut(s) 515, 570, 741, 1253, 1357, 1366, 1540
HincII GTYRAC 2 cut(s) 141, 1351
HindII GTYRAC 2 cut(s) 141, 1351
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 7 cut(s) 200, 499, 808, 1112, 1205, 1550, 1615
HpaI GTTAAC 1 cut(s) 141
HpaII CCGG 2 cut(s) 14, 853
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1351, 1670
Hpy188III TCNNGA 9 cut(s) 28, 302, 689, 853, 956, 1199, 1238, 1283, 1496
Hpy8I GTNNAC 7 cut(s) 141, 152, 199, 607, 864, 1351, 1670
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 5 cut(s) 388, 976, 1093, 1353, 1458
HpyCH4III ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
HpyCH4IV ACGT 3 cut(s) 195, 673, 1602
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 350, 377
HpyF3I CTNAG 7 cut(s) 135, 202, 300, 633, 834, 949, 1773
HpySE526I ACGT 3 cut(s) 195, 673, 1602
Hsp92II CATG 7 cut(s) 515, 570, 741, 1253, 1357, 1366, 1540
Kpn2I TCCGGA 1 cut(s) 852
Ksp22I TGATCA 2 cut(s) 1489, 1713
KspAI GTTAAC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 1192, 1297, 1586
Lsp1109I GCAGC 6 cut(s) 227, 319, 396, 1225, 1228, 1287
LweI GCATC 3 cut(s) 408, 602, 656
MaeI CTAG 7 cut(s) 63, 288, 663, 764, 1224, 1283, 1563
MaeII ACGT 3 cut(s) 195, 673, 1602
MaeIII GTNAC 3 cut(s) 191, 886, 1532
MflI RGATCY 3 cut(s) 304, 1727, 1764
MhlI GDGCHC 3 cut(s) 154, 1583, 1687
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 5 cut(s) 31, 315, 1030, 1179, 1691
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 1 cut(s) 194
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 628
MroI TCCGGA 1 cut(s) 852
MroXI GAANNNNTTC 3 cut(s) 1006, 1389, 1468
MscI TGGCCA 1 cut(s) 270
MseI TTAA 4 cut(s) 140, 318, 1622, 1664
MslI CAYNNNNRTG 3 cut(s) 463, 558, 1224
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 218, 726
MspI CCGG 2 cut(s) 14, 853
MspR9I CCNGG 5 cut(s) 15, 473, 602, 709, 1588
Mva1269I GAATGC 1 cut(s) 1633
MvaI CCWGG 4 cut(s) 473, 602, 709, 1588
MwoI GCNNNNNNNGC 3 cut(s) 341, 350, 377
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 1362
NlaIII CATG 7 cut(s) 515, 570, 741, 1253, 1357, 1366, 1540
NlaIV GGNNCC 5 cut(s) 696, 750, 1015, 1542, 1766
NmeAIII GCCGAG 1 cut(s) 683
NmuCI GTSAC 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 4 cut(s) 515, 570, 1253, 1357
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 1224
PciI ACATGT 2 cut(s) 566, 1249
PctI GAATGC 1 cut(s) 1633
PdmI GAANNNNTTC 3 cut(s) 1006, 1389, 1468
PfeI GAWTC 6 cut(s) 499, 808, 1112, 1205, 1550, 1615
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 6 cut(s) 217, 334, 411, 1215, 1218, 1302
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 694
PscI ACATGT 2 cut(s) 566, 1249
PshAI GACNNNNGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 1583
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 4 cut(s) 471, 600, 707, 1586
PspGI CCWGG 4 cut(s) 471, 600, 707, 1586
PspN4I GGNNCC 5 cut(s) 696, 750, 1015, 1542, 1766
PspPI GGNCC 5 cut(s) 694, 729, 875, 1013, 1444
PspPPI RGGWCCY 1 cut(s) 694
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PstI CTGCAG 1 cut(s) 1423
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 3 cut(s) 304, 1727, 1764
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 1171
RsaNI GTAC 1 cut(s) 1170
RseI CAYNNNNRTG 3 cut(s) 463, 558, 1224
SacI GAGCTC 1 cut(s) 1583
SaqAI TTAA 4 cut(s) 140, 318, 1622, 1664
SatI GCNGC 6 cut(s) 216, 333, 410, 1214, 1217, 1301
Sau96I GGNCC 5 cut(s) 694, 729, 875, 1013, 1444
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 5 cut(s) 15, 473, 602, 709, 1588
SduI GDGCHC 3 cut(s) 154, 1583, 1687
SfaNI GCATC 3 cut(s) 408, 602, 656
SfcI CTRYAG 1 cut(s) 1419
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 4 cut(s) 694, 729, 1013, 1444
SmiMI CAYNNNNRTG 3 cut(s) 463, 558, 1224
SmlI CTYRAG 1 cut(s) 1698
SmoI CTYRAG 1 cut(s) 1698
SpeI ACTAGT 1 cut(s) 1223
Sse9I AATT 5 cut(s) 31, 315, 1030, 1179, 1691
SsiI CCGC 1 cut(s) 724
SspMI CTAG 7 cut(s) 63, 288, 663, 764, 1224, 1283, 1563
SstI GAGCTC 1 cut(s) 1583
StyD4I CCNGG 5 cut(s) 13, 471, 600, 707, 1586
StyI CCWWGG 4 cut(s) 1017, 1074, 1116, 1362
TaaI ACNGT 7 cut(s) 109, 145, 184, 817, 850, 861, 1169
TaiI ACGT 3 cut(s) 198, 676, 1605
TaqI TCGA 4 cut(s) 56, 385, 573, 617
TasI AATT 5 cut(s) 31, 315, 1030, 1179, 1691
TatI WGTACW 1 cut(s) 1169
TfiI GAWTC 6 cut(s) 499, 808, 1112, 1205, 1550, 1615
Tru1I TTAA 4 cut(s) 140, 318, 1622, 1664
Tru9I TTAA 4 cut(s) 140, 318, 1622, 1664
TscAI CASTG 4 cut(s) 114, 154, 501, 871
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 6 cut(s) 215, 332, 409, 1213, 1216, 1300
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 3 cut(s) 240, 959, 1398
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 4 cut(s) 114, 154, 501, 871
Van91I CCANNNNNTGG 1 cut(s) 176
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 4 cut(s) 694, 729, 1013, 1444
XapI RAATTY 1 cut(s) 31
XbaI TCTAGA 1 cut(s) 1282
XceI RCATGY 4 cut(s) 515, 570, 1253, 1357
XmnI GAANNNNTTC 3 cut(s) 1006, 1389, 1468
XspI CTAG 7 cut(s) 63, 288, 663, 764, 1224, 1283, 1563
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.