FvH4_4g32190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015609
Physical Location & Seq
Reverse (-)
30950506 .. 30951360
855 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32190.t1

Sequence Viewer

Length: 471 bp
ATGGCGACTACTTCTTCTCTTTGCTCGGCTGTCTCACCGGAACCGTGGTTCAGTTCCGGCAACCAGGCGTGTCCCAAGACGGGACTTGTTTGTTTGCAGAGGAGACGACGACGCCCCATGAAGGTTTCTGCGTCGTATGATCGTGACAATCACGACGGTAAACTTGTTGACGAGGACATGATTGTTCTTAGGATGAGGATTCATGATATTGAGATGACTGAGACGAGTGATCGGAACGAAATGCCGTCGCATTGGATGGAATGGGAGAAGCGATACCATGCGCATTACAATTCAGATGTCTATGAAGCCGTCGGGTTGTTGCAGTCCCAATTGATGAAAACGAGGCCTTGCTTAGCTTTGGGAATACTTGCTCTTTTTACACTTAGCGTGCCGGTCACCACTGCTGCTTTGATGTCCCCTCTGTTCGACATCGTTAAGGGTATATTATTAGCCAACGGGATTCAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.62

Weight (kDa)

6.95

Isoelectric Point (pI)

66.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015609)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32190
malus_domestica MD13G1027400.v1.1
prunus_persica Prupe.1G322500_v2.0.a1
pyrus_communis pycom16g02650 pycom16g02660
rosa_chinensis RchiOBHm_Chr4g0440931
rosa_laevigata RLG00000006139
rosa_multiflora Rmu_sc0000424.1_g000023 Rmu_sc0005054.1_g000002
rosa_roxburghii Rroxscaffold_5G00381790
rosa_rugosa Rorug04G0329300
rosa_samantha Rh4AG381200 Rh4BG394400 Rh4CG409300 Rh4DG387700
rosa_wichuraiana Rw4G032830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 80, 121
AgsI TTSAA 1 cut(s) 464
AjnI CCWGG 1 cut(s) 63
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 1 cut(s) 356
AluI AGCT 1 cut(s) 356
Alw26I GTCTC 3 cut(s) 37, 97, 215
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 27, 388
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 250
BceAI ACGGC 2 cut(s) 229, 293
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 37, 97, 215
BisI GCNGC 1 cut(s) 405
BlpI GCTNAGC 1 cut(s) 352
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 44
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 121
Bse118I RCCGGY 1 cut(s) 391
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 2 cut(s) 198, 261
BseLI CCNNNNNNNGG 2 cut(s) 80, 121
BseMII CTCAG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 1 cut(s) 391
BshFI GGCC 1 cut(s) 346
BsiSI CCGG 3 cut(s) 38, 57, 392
BslFI GGGAC 4 cut(s) 57, 96, 310, 400
BslI CCNNNNNNNGG 2 cut(s) 80, 121
BsmAI GTCTC 3 cut(s) 37, 97, 215
BsmBI CGTCTC 2 cut(s) 97, 215
BsmFI GGGAC 4 cut(s) 57, 96, 310, 400
BsnI GGCC 1 cut(s) 346
Bsp143I GATC 2 cut(s) 139, 229
Bsp1720I GCTNAGC 1 cut(s) 352
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 211
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 44
BssMI GATC 2 cut(s) 139, 229
BssNI GRCGYC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 2 cut(s) 45, 158
BstACI GRCGYC 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 4 cut(s) 188, 219, 352, 383
BstDSI CCRYGG 1 cut(s) 44
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 2 cut(s) 198, 261
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 142, 232
BstMAI GTCTC 3 cut(s) 37, 97, 215
BstMBI GATC 2 cut(s) 139, 229
BstNI CCWGG 1 cut(s) 65
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 346
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 2 cut(s) 198, 261
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 389
CciI TCATGA 1 cut(s) 202
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 391
CseI GACGC 2 cut(s) 120, 120
CviAII CATG 4 cut(s) 118, 178, 203, 278
CviJI RGCY 5 cut(s) 29, 308, 346, 356, 452
CviKI_1 RGCY 5 cut(s) 29, 308, 346, 356, 452
DdeI CTNAG 4 cut(s) 188, 219, 352, 383
DpnI GATC 2 cut(s) 141, 231
DpnII GATC 2 cut(s) 139, 229
Eco147I AGGCCT 1 cut(s) 346
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 63
Esp3I CGTCTC 2 cut(s) 97, 215
FaeI CATG 4 cut(s) 121, 181, 206, 281
FaiI YATR 7 cut(s) 119, 138, 179, 204, 279, 303, 443
FaqI GGGAC 4 cut(s) 57, 96, 310, 400
FatI CATG 4 cut(s) 117, 177, 202, 277
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 2 cut(s) 205, 268
Fsp4HI GCNGC 1 cut(s) 405
FspAI RTGCGCAY 1 cut(s) 282
FspI TGCGCA 1 cut(s) 282
GlaI GCGC 1 cut(s) 282
GluI GCNGC 1 cut(s) 405
HaeIII GGCC 1 cut(s) 346
HapII CCGG 3 cut(s) 38, 57, 392
HgaI GACGC 2 cut(s) 120, 120
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 112
Hin1II CATG 4 cut(s) 121, 181, 206, 281
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 2 cut(s) 199, 460
HpaII CCGG 3 cut(s) 38, 57, 392
HphI GGTGA 2 cut(s) 27, 388
Hpy166II GTNNAC 2 cut(s) 161, 169
Hpy188I TCNGA 2 cut(s) 234, 295
Hpy188III TCNNGA 3 cut(s) 143, 152, 203
Hpy8I GTNNAC 2 cut(s) 161, 169
Hpy99I CGWCG 6 cut(s) 111, 114, 136, 158, 250, 314
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 45, 158
HpyCH4V TGCA 2 cut(s) 97, 322
HpyF3I CTNAG 4 cut(s) 188, 219, 352, 383
Hsp92I GRCGYC 1 cut(s) 112
Hsp92II CATG 4 cut(s) 121, 181, 206, 281
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 2 cut(s) 139, 229
LpnPI CCDG 5 cut(s) 50, 51, 70, 77, 405
Lsp1109I GCAGC 1 cut(s) 391
MaeIII GTNAC 2 cut(s) 143, 394
MalI GATC 2 cut(s) 141, 231
MboI GATC 2 cut(s) 139, 229
MboII GAAGA 1 cut(s) 6
MfeI CAATTG 1 cut(s) 329
MluCI AATT 2 cut(s) 289, 329
MnlI CCTC 5 cut(s) 93, 166, 189, 336, 429
MseI TTAA 1 cut(s) 435
MspI CCGG 3 cut(s) 38, 57, 392
MspR9I CCNGG 1 cut(s) 65
MunI CAATTG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 65
NdeII GATC 2 cut(s) 139, 229
NlaIII CATG 4 cut(s) 121, 181, 206, 281
NlaIV GGNNCC 1 cut(s) 42
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 2 cut(s) 143, 394
NsbI TGCGCA 1 cut(s) 282
PagI TCATGA 1 cut(s) 202
PceI AGGCCT 1 cut(s) 346
PfeI GAWTC 2 cut(s) 199, 460
PkrI GCNGC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 42
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 2 cut(s) 139, 229
ScrFI CCNGG 1 cut(s) 65
SetI ASST 2 cut(s) 126, 358
Sse9I AATT 2 cut(s) 289, 329
SseBI AGGCCT 1 cut(s) 346
StuI AGGCCT 1 cut(s) 346
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 45, 158
TaqI TCGA 1 cut(s) 426
TasI AATT 2 cut(s) 289, 329
TfiI GAWTC 2 cut(s) 199, 460
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 143, 394
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 2 cut(s) 143, 394
TspDTI ATGAA 4 cut(s) 134, 191, 318, 350
TspRI CASTG 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.