Rorug04G0329300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015609
Physical Location & Seq
Reverse (-)
50367967 .. 50369360
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0329300.1

Sequence Viewer

Length: 1083 bp
ATGCTGATCAGGACAGTGATCAGTGAGGTCCAGACTATGGTCGGCCAGTTGATACCTCAAAAACTCCAGGACAAAATCATTGCCACACTTGCAGGCCTATTCGGTAACCTTCCTAATTCTCAAATGACTCTTATCATTGATGAAAGCAATGGTCTCTCTATCAATGAAGTTTACCAGGATTCAGAACTCTACTTAAGCACAAGGATCACTGCCTCAATTGATCAACTTAAGGTTTCCAAGTCCCCACGCCAAAACAACTTCTCTGTTACCATAAACAAAGGCGAGAAGCTAACTGACGAGTTCGAAGGAATGCAGTTGAGGTGGGAATTCTTGCAAAGATCAAAAGCAATTAAAGAGGAAAACAAGGTGGTGAGGCTGCATGCACTTGGAAACTTTGGTGGAGAATGTTCGATCGGTCCCTGGGGTTCAGTCATCTTTGAACATCCATCAACTTTTGACACATTGGCAATGGACCCAATGCTGAAGAACGAGGTGATTGCTGACTTGGACAGGTTTGTGAAGCGAAAGGAATTTTATGGCAGAGTAGGCAAGGCATGGAAACGAGGGTACTTGTTGTATGGTCCTCCTGGCACAGGAAAGTCGAGCTTGATTGCAGCCATGGCTAATCACCTCAACATCGAATTGAATGACAGACAAGTTAGAGGAACCAACCCCAGTGACAGCCAGTTGACACTATCCGGATTGCTCAATTTCATTGATGGTTTATGGTCAAGCTGTGGAGATGAGAGAATAATTGTGTTTACCACCAACTACAAGGACAAGCTAGATCCTGCATTGTTGAGACCAGGCCGAATGGATATGCATGTTCACATGTCCTACTGCACACCATGTGGATTCAAAACCCTTGCTCATAACTACTTTAAAGTAAAAACTCATCATCTCTTCAATGAAATTGAAAAATTCATAAAGGAGGTTGATACGACTCCAGCAGAGATTGCAGAAGAACTCATGAAAAGTGAAGACACCGATATAGCGCTGGGAGGACTCATTGCATTCCTGAAGAAGAAGAAAGAGATGAACTGCAGTGCTGAAGCCAACGTTGAAAAAAACAATAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

40.58

Weight (kDa)

7.09

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 18 - 111 8e-23 Domain associated at C-terminal with AAA
AAA PF00004 222 - 279 3.4e-06 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 281 - 349 4.6e-24 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015609)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32190
malus_domestica MD13G1027400.v1.1
prunus_persica Prupe.1G322500_v2.0.a1
pyrus_communis pycom16g02650 pycom16g02660
rosa_chinensis RchiOBHm_Chr4g0440931
rosa_laevigata RLG00000006139
rosa_multiflora Rmu_sc0000424.1_g000023 Rmu_sc0005054.1_g000002
rosa_roxburghii Rroxscaffold_5G00381790
rosa_rugosa Rorug04G0329300
rosa_samantha Rh4AG381200 Rh4BG394400 Rh4CG409300 Rh4DG387700
rosa_wichuraiana Rw4G032830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 37
AccIII TCCGGA 1 cut(s) 698
AclI AACGTT 1 cut(s) 1059
AclWI GGATC 2 cut(s) 212, 782
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 3 cut(s) 326, 530, 920
AcuI CTGAAG 3 cut(s) 503, 1040, 1071
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 1 cut(s) 569
AfeI AGCGCT 1 cut(s) 996
AfiI CCNNNNNNNGG 2 cut(s) 37, 593
AflII CTTAAG 2 cut(s) 193, 227
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 6 cut(s) 440, 646, 859, 907, 917, 1064
AjnI CCWGG 5 cut(s) 66, 174, 419, 586, 805
AluBI AGCT 4 cut(s) 289, 606, 735, 784
AluI AGCT 4 cut(s) 289, 606, 735, 784
Alw26I GTCTC 2 cut(s) 158, 796
AlwI GGATC 2 cut(s) 212, 782
Aor13HI TCCGGA 1 cut(s) 698
Aor51HI AGCGCT 1 cut(s) 996
AoxI GGCC 3 cut(s) 43, 94, 808
ApeKI GCWGC 2 cut(s) 376, 614
ApoI RAATTY 3 cut(s) 326, 530, 920
AspLEI GCGC 1 cut(s) 997
AspS9I GGNCC 4 cut(s) 28, 416, 472, 581
AsuHPI GGTGA 3 cut(s) 382, 505, 620
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 4 cut(s) 28, 416, 472, 581
BbsI GAAGAC 1 cut(s) 987
BbvI GCAGC 2 cut(s) 363, 626
BccI CCATC 2 cut(s) 454, 713
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 5 cut(s) 68, 176, 421, 588, 807
BclI TGATCA 3 cut(s) 6, 18, 220
BcoDI GTCTC 2 cut(s) 158, 796
BfaI CTAG 1 cut(s) 785
BfmI CTRYAG 1 cut(s) 1042
BfoI RGCGCY 1 cut(s) 998
BfrI CTTAAG 2 cut(s) 193, 227
BisI GCNGC 2 cut(s) 377, 615
BlsI GCNGC 2 cut(s) 378, 616
Bme1390I CCNGG 5 cut(s) 68, 176, 421, 588, 807
Bme18I GGWCC 4 cut(s) 28, 416, 472, 581
BmgT120I GGNCC 4 cut(s) 28, 416, 472, 581
BmiI GGNNCC 3 cut(s) 418, 474, 667
BmrFI CCNGG 5 cut(s) 68, 176, 421, 588, 807
BmrI ACTGGG 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 669
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 987
BpmI CTGGAG 2 cut(s) 50, 930
Bpu14I TTCGAA 1 cut(s) 303
BsaI GGTCTC 2 cut(s) 158, 796
BsaJI CCNNGG 3 cut(s) 419, 420, 618
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 593
Bse1I ACTGG 3 cut(s) 46, 675, 685
Bse3DI GCAATG 4 cut(s) 78, 154, 474, 1008
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 5 cut(s) 68, 176, 421, 588, 807
BseDI CCNNGG 3 cut(s) 419, 420, 618
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 37, 593
BseMI GCAATG 4 cut(s) 78, 154, 474, 1008
BseNI ACTGG 3 cut(s) 46, 675, 685
BseXI GCAGC 2 cut(s) 363, 626
BseYI CCCAGC 1 cut(s) 997
BsgI GTGCAG 1 cut(s) 826
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 3 cut(s) 45, 96, 810
BsiEI CGRYCG 1 cut(s) 414
BsiSI CCGG 1 cut(s) 699
BslFI GGGAC 2 cut(s) 226, 402
BslI CCNNNNNNNGG 2 cut(s) 37, 593
BsmAI GTCTC 2 cut(s) 158, 796
BsmFI GGGAC 2 cut(s) 226, 402
BsmI GAATGC 2 cut(s) 315, 1013
BsnI GGCC 3 cut(s) 45, 96, 810
Bso31I GGTCTC 2 cut(s) 158, 796
Bsp119I TTCGAA 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 698
Bsp143I GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
Bsp19I CCATGG 1 cut(s) 618
BspANI GGCC 3 cut(s) 45, 96, 810
BspEI TCCGGA 1 cut(s) 698
BspHI TCATGA 1 cut(s) 969
BspLI GGNNCC 3 cut(s) 418, 474, 667
BspMAI CTGCAG 1 cut(s) 1046
BspPI GGATC 2 cut(s) 212, 782
BspT104I TTCGAA 1 cut(s) 303
BspTI CTTAAG 2 cut(s) 193, 227
BspTNI GGTCTC 2 cut(s) 158, 796
BsrDI GCAATG 4 cut(s) 78, 154, 474, 1008
BsrI ACTGG 3 cut(s) 46, 675, 685
BssECI CCNNGG 3 cut(s) 419, 420, 618
BssMI GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 5 cut(s) 68, 176, 421, 588, 807
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 1 cut(s) 908
BstAFI CTTAAG 2 cut(s) 193, 227
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 94, 381
BstDSI CCRYGG 1 cut(s) 618
BstEII GGTNACC 1 cut(s) 104
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 1 cut(s) 442
BstH2I RGCGCY 1 cut(s) 998
BstHHI GCGC 1 cut(s) 997
BstKTI GATC 7 cut(s) 9, 21, 207, 223, 341, 414, 790
BstMAI GTCTC 2 cut(s) 158, 796
BstMBI GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 4 cut(s) 89, 546, 620, 956
BstNI CCWGG 5 cut(s) 68, 176, 421, 588, 807
BstNSI RCATGY 3 cut(s) 383, 827, 835
BstPAI GACNNNNGTC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 104
BstSCI CCNGG 5 cut(s) 66, 174, 419, 586, 805
BstSFI CTRYAG 1 cut(s) 1042
BstV1I GCAGC 2 cut(s) 363, 626
BstV2I GAAGAC 1 cut(s) 987
BstX2I RGATCY 1 cut(s) 787
BstYI RGATCY 1 cut(s) 787
BsuRI GGCC 3 cut(s) 45, 96, 810
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 1 cut(s) 442
BtsI GCAGTG 2 cut(s) 207, 1051
BtsIMutI CAGTG 5 cut(s) 21, 28, 207, 682, 1051
Cac8I GCNNGC 2 cut(s) 94, 381
CciI TCATGA 1 cut(s) 969
CfoI GCGC 1 cut(s) 997
Cfr13I GGNCC 4 cut(s) 28, 416, 472, 581
Csp6I GTAC 1 cut(s) 568
CviAII CATG 7 cut(s) 380, 555, 619, 824, 832, 849, 970
CviQI GTAC 1 cut(s) 568
DpnI GATC 7 cut(s) 8, 20, 206, 222, 340, 413, 789
DpnII GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
DraI TTTAAA 1 cut(s) 883
DraIII CACNNNGTG 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 908
EarI CTCTTC 1 cut(s) 908
Eco130I CCWWGG 1 cut(s) 618
Eco147I AGGCCT 1 cut(s) 96
Eco31I GGTCTC 2 cut(s) 158, 796
Eco47I GGWCC 4 cut(s) 28, 416, 472, 581
Eco47III AGCGCT 1 cut(s) 996
Eco57I CTGAAG 3 cut(s) 503, 1040, 1071
Eco91I GGTNACC 1 cut(s) 104
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoO65I GGTNACC 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 5 cut(s) 66, 174, 419, 586, 805
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 7 cut(s) 383, 558, 622, 827, 835, 852, 973
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FaqI GGGAC 2 cut(s) 226, 402
FatI CATG 7 cut(s) 379, 554, 618, 823, 831, 848, 969
FbaI TGATCA 3 cut(s) 6, 18, 220
Fnu4HI GCNGC 2 cut(s) 377, 615
FokI GGATG 1 cut(s) 429
Fsp4HI GCNGC 2 cut(s) 377, 615
FspBI CTAG 1 cut(s) 785
GlaI GCGC 1 cut(s) 996
GluI GCNGC 2 cut(s) 377, 615
GsaI CCCAGC 1 cut(s) 1001
GsuI CTGGAG 2 cut(s) 50, 930
HaeII RGCGCY 1 cut(s) 998
HaeIII GGCC 3 cut(s) 45, 96, 810
HapII CCGG 1 cut(s) 699
HhaI GCGC 1 cut(s) 997
Hin1II CATG 7 cut(s) 383, 558, 622, 827, 835, 852, 973
Hin6I GCGC 1 cut(s) 995
HinP1I GCGC 1 cut(s) 995
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HinfI GANTC 5 cut(s) 127, 179, 855, 943, 1005
HpaII CCGG 1 cut(s) 699
HphI GGTGA 3 cut(s) 382, 505, 620
Hpy166II GTNNAC 4 cut(s) 172, 690, 762, 829
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 5 cut(s) 10, 31, 699, 970, 1018
Hpy8I GTNNAC 4 cut(s) 172, 690, 762, 829
HpyAV CCTTC 2 cut(s) 119, 299
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 1 cut(s) 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 546, 620, 956
HpySE526I ACGT 1 cut(s) 1059
Hsp92II CATG 7 cut(s) 383, 558, 622, 827, 835, 852, 973
HspAI GCGC 1 cut(s) 995
Kpn2I TCCGGA 1 cut(s) 698
Ksp22I TGATCA 3 cut(s) 6, 18, 220
Kzo9I GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
Lsp1109I GCAGC 2 cut(s) 363, 626
MaeI CTAG 1 cut(s) 785
MaeII ACGT 1 cut(s) 1059
MaeIII GTNAC 3 cut(s) 104, 265, 677
MalI GATC 7 cut(s) 8, 20, 206, 222, 340, 413, 789
MboI GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
MboII GAAGA 7 cut(s) 496, 895, 974, 992, 1033, 1036, 1039
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 1 cut(s) 787
MlyI GAGTC 3 cut(s) 121, 937, 999
Mph1103I ATGCAT 1 cut(s) 825
MroI TCCGGA 1 cut(s) 698
MseI TTAA 4 cut(s) 194, 228, 351, 882
MspCI CTTAAG 2 cut(s) 193, 227
MspI CCGG 1 cut(s) 699
MspR9I CCNGG 5 cut(s) 68, 176, 421, 588, 807
MunI CAATTG 1 cut(s) 216
Mva1269I GAATGC 2 cut(s) 315, 1013
MvaI CCWGG 5 cut(s) 68, 176, 421, 588, 807
MwoI GCNNNNNNNGC 4 cut(s) 89, 546, 620, 956
NcoI CCATGG 1 cut(s) 618
NdeII GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
NlaIII CATG 7 cut(s) 383, 558, 622, 827, 835, 852, 973
NlaIV GGNNCC 3 cut(s) 418, 474, 667
NmuCI GTSAC 1 cut(s) 677
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 3 cut(s) 383, 827, 835
NspV TTCGAA 1 cut(s) 303
PaeI GCATGC 1 cut(s) 383
PagI TCATGA 1 cut(s) 969
PasI CCCWGGG 1 cut(s) 420
PceI AGGCCT 1 cut(s) 96
PciI ACATGT 1 cut(s) 831
PctI GAATGC 2 cut(s) 315, 1013
PfeI GAWTC 2 cut(s) 179, 855
PflMI CCANNNNNTGG 1 cut(s) 37
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 2 cut(s) 378, 616
Ple19I CGATCG 1 cut(s) 414
PleI GAGTC 3 cut(s) 121, 937, 999
PpsI GAGTC 3 cut(s) 121, 937, 999
PscI ACATGT 1 cut(s) 831
PshAI GACNNNNGTC 1 cut(s) 38
Psp1406I AACGTT 1 cut(s) 1059
Psp6I CCWGG 5 cut(s) 66, 174, 419, 586, 805
PspEI GGTNACC 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 997
PspGI CCWGG 5 cut(s) 66, 174, 419, 586, 805
PspN4I GGNNCC 3 cut(s) 418, 474, 667
PspPI GGNCC 4 cut(s) 28, 416, 472, 581
PstI CTGCAG 1 cut(s) 1046
PsuI RGATCY 1 cut(s) 787
PvuI CGATCG 1 cut(s) 414
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SaqAI TTAA 4 cut(s) 194, 228, 351, 882
SatI GCNGC 2 cut(s) 377, 615
Sau3AI GATC 7 cut(s) 6, 18, 204, 220, 338, 411, 787
Sau96I GGNCC 4 cut(s) 28, 416, 472, 581
SchI GAGTC 3 cut(s) 121, 937, 999
ScrFI CCNGG 5 cut(s) 68, 176, 421, 588, 807
SfcI CTRYAG 1 cut(s) 1042
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 4 cut(s) 28, 416, 472, 581
SmlI CTYRAG 2 cut(s) 193, 227
SmoI CTYRAG 2 cut(s) 193, 227
SphI GCATGC 1 cut(s) 383
SseBI AGGCCT 1 cut(s) 96
SspMI CTAG 1 cut(s) 785
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 5 cut(s) 66, 174, 419, 586, 805
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 1 cut(s) 1062
TaqI TCGA 4 cut(s) 303, 410, 602, 639
TaqII GACCGA 1 cut(s) 404
TfiI GAWTC 2 cut(s) 179, 855
Tru1I TTAA 4 cut(s) 194, 228, 351, 882
Tru9I TTAA 4 cut(s) 194, 228, 351, 882
TscAI CASTG 5 cut(s) 21, 28, 214, 682, 1051
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 2 cut(s) 376, 614
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 7 cut(s) 156, 180, 703, 913, 924, 986, 1052
TspRI CASTG 5 cut(s) 21, 28, 214, 682, 1051
Van91I CCANNNNNTGG 1 cut(s) 37
Vha464I CTTAAG 2 cut(s) 193, 227
VpaK11BI GGWCC 4 cut(s) 28, 416, 472, 581
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 3 cut(s) 326, 530, 920
XceI RCATGY 3 cut(s) 383, 827, 835
XspI CTAG 1 cut(s) 785
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.