FvH4_4g32640

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014313
Physical Location & Seq
Forward (+)
31230403 .. 31234499
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32640.t1

Sequence Viewer

Length: 852 bp
ATGGGAATCGCCGGCAATCTCAGTTCGGACCAAGTCCAGTTCTTCAACTCCCAAGGTTATCTTGTACTGGAATCGTTCGCCACTCCTGAGGAAATCGAAGCCATGAGGACGAGGATGGACCAGTTGCTCCACGACTTTGACTGCTCCGCCACCGCCTCTATTTTCTCTACCAAGAACCAGCAACAGTTGACCGACAACTATTTCTTGGAAAGTGTCGACAAGATTTCGTTTTTCTTCGAAGAGAAAGCTTTTGGGGATGATGGAAGCCTAAAGCAGCCAAAGGGACTTTCCATCAATAAAGTTGGACATGCATTACATGAGCTTGATCCGGTATTCAAAAAGTTCTCCTATTCTGAGAAAGTTTCGGGTTTGTTATCCTCCTTGGCATACAAAAGGCCCATTCCCATTCAGTCTATGTATCTCTTTAAGCAACCGGGTATTGGCGGTGAGGTAGTGCCACACCAGGATAACTCATTTCTTTATACCGAACCACAAACTTGCACTGGGCTGTGGCTAGCCTTGGAAGATGCAACAGTAGTAAATGGCTGCCTGTGGGCTATTCCTGGATCTCAAAAGAATGGCCTTGTTAGACGGTTGATTAGAGGTGAAGACGGTATGACCTTTGATCGTCCGTCCCCTGTGTATGATCCAAAAGATTTTGTGCCTATTGAAGTCAAAGCTGGGTCTTTAGTTGTTATTCATGGTGATCTTATTCATCAAAGTTTCGAGAACCAGTCTTCAAAGTCAAGGCATGCATATAGCGTGCATGTGGTGGATACAGATGGCTGCAAATGGGCTCCAGATAATTGGATTCGGCGGAAGGTGGAACCAGAGCCCATCTATGTATCTTAA

Protein Analysis

284

Amino Acids

31.73

Weight (kDa)

5.38

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 15 - 252 9.3e-54 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01490 AT2G01490
fragaria_vesca FvH4_4g32640
malus_domestica MD13G1023900.v1.1 MD16G1026600.v1.1
prunus_persica Prupe.1G326800_v2.0.a1
pyrus_communis pycom16g02240
rosa_chinensis RchiOBHm_Chr4g0441521
rosa_laevigata RLG00000006088
rosa_roxburghii Rroxscaffold_5G00382370 Rroxscaffold_7G00185860
rosa_rugosa Rorug04G0334400 Rorug06G0150600
rosa_samantha Rh4BG399900 Rh4CG414900
rosa_wichuraiana Rw4G033320 Rw6G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AciI CCGC 4 cut(s) 147, 153, 444, 817
AclWI GGATC 3 cut(s) 320, 574, 641
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 4 cut(s) 46, 337, 671, 741
AjnI CCWGG 2 cut(s) 462, 562
AjuI GAANNNNNNNTTGG 2 cut(s) 271, 303
AluBI AGCT 3 cut(s) 248, 322, 680
AluI AGCT 3 cut(s) 248, 322, 680
AlwI GGATC 3 cut(s) 320, 574, 641
AoxI GGCC 2 cut(s) 395, 580
ApeKI GCWGC 3 cut(s) 274, 546, 786
AspS9I GGNCC 3 cut(s) 28, 118, 396
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 458, 617, 716
AsuII TTCGAA 1 cut(s) 237
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 2 cut(s) 28, 118
AxyI CCTNAGG 1 cut(s) 87
BanII GRGCYC 2 cut(s) 799, 837
BbsI GAAGAC 2 cut(s) 615, 729
BbvI GCAGC 3 cut(s) 286, 533, 773
BccI CCATC 5 cut(s) 109, 254, 299, 776, 845
BciT130I CCWGG 2 cut(s) 464, 564
BciVI GTATCC 1 cut(s) 769
BcnI CCSGG 1 cut(s) 435
BfaI CTAG 1 cut(s) 515
BfuI GTATCC 1 cut(s) 769
BisI GCNGC 3 cut(s) 275, 547, 787
BlsI GCNGC 3 cut(s) 276, 548, 788
Bme1390I CCNGG 3 cut(s) 435, 464, 564
Bme18I GGWCC 2 cut(s) 28, 118
BmgT120I GGNCC 3 cut(s) 28, 118, 396
BmiI GGNNCC 2 cut(s) 798, 828
BmrFI CCNGG 3 cut(s) 435, 464, 564
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 1 cut(s) 517
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 2 cut(s) 615, 729
BpmI CTGGAG 1 cut(s) 783
Bpu14I TTCGAA 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 52, 381, 519
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 5 cut(s) 37, 72, 121, 508, 733
Bse21I CCTNAGG 1 cut(s) 87
BseBI CCWGG 2 cut(s) 464, 564
BseDI CCNNGG 3 cut(s) 52, 381, 519
BseGI GGATG 2 cut(s) 120, 262
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 3 cut(s) 34, 78, 345
BseNI ACTGG 5 cut(s) 37, 72, 121, 508, 733
BseXI GCAGC 3 cut(s) 286, 533, 773
BseYI CCCAGC 1 cut(s) 680
BshFI GGCC 2 cut(s) 397, 582
BsiSI CCGG 3 cut(s) 12, 329, 434
BslFI GGGAC 2 cut(s) 297, 619
BslI CCNNNNNNNGG 1 cut(s) 440
BsmFI GGGAC 2 cut(s) 297, 619
BsnI GGCC 2 cut(s) 397, 582
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 799, 837
Bsp143I GATC 5 cut(s) 325, 566, 625, 646, 706
BspACI CCGC 4 cut(s) 147, 153, 444, 817
BspANI GGCC 2 cut(s) 397, 582
BspCNI CTCAG 3 cut(s) 33, 79, 346
BspLI GGNNCC 2 cut(s) 798, 828
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 3 cut(s) 320, 574, 641
BspT104I TTCGAA 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 5 cut(s) 37, 72, 121, 508, 733
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 52, 381, 519
BssMI GATC 5 cut(s) 325, 566, 625, 646, 706
BssT1I CCWWGG 3 cut(s) 52, 381, 519
Bst2UI CCWGG 2 cut(s) 464, 564
Bst4CI ACNGT 4 cut(s) 186, 535, 594, 614
Bst6I CTCTTC 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 13, 516, 753, 764
BstDEI CTNAG 3 cut(s) 20, 87, 354
BstF5I GGATG 2 cut(s) 120, 262
BstKTI GATC 5 cut(s) 328, 569, 628, 649, 709
BstMBI GATC 5 cut(s) 325, 566, 625, 646, 706
BstNI CCWGG 2 cut(s) 464, 564
BstNSI RCATGY 3 cut(s) 311, 755, 770
BstSCI CCNGG 3 cut(s) 433, 462, 562
BstV1I GCAGC 3 cut(s) 286, 533, 773
BstV2I GAAGAC 2 cut(s) 615, 729
BstX2I RGATCY 1 cut(s) 566
BstXI CCANNNNNNTGG 1 cut(s) 807
BstYI RGATCY 1 cut(s) 566
Bsu36I CCTNAGG 1 cut(s) 87
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 2 cut(s) 397, 582
BtsCI GGATG 2 cut(s) 120, 262
BtsIMutI CAGTG 1 cut(s) 501
Cac8I GCNNGC 4 cut(s) 13, 516, 753, 764
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 28, 118, 396
Csp6I GTAC 1 cut(s) 65
CviAII CATG 6 cut(s) 103, 308, 317, 701, 752, 767
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 3 cut(s) 20, 87, 354
DpnI GATC 5 cut(s) 327, 568, 627, 648, 708
DpnII GATC 5 cut(s) 325, 566, 625, 646, 706
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 2 cut(s) 136, 832
Eco130I CCWWGG 3 cut(s) 52, 381, 519
Eco24I GRGCYC 2 cut(s) 799, 837
Eco47I GGWCC 2 cut(s) 28, 118
Eco81I CCTNAGG 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 462, 562
EcoT14I CCWWGG 3 cut(s) 52, 381, 519
EcoT22I ATGCAT 2 cut(s) 313, 757
EcoT38I GRGCYC 2 cut(s) 799, 837
ErhI CCWWGG 3 cut(s) 52, 381, 519
FaeI CATG 6 cut(s) 106, 311, 320, 704, 755, 770
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 297, 619
FatI CATG 6 cut(s) 102, 307, 316, 700, 751, 766
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 275, 547, 787
FokI GGATG 2 cut(s) 127, 269
FriOI GRGCYC 2 cut(s) 799, 837
Fsp4HI GCNGC 3 cut(s) 275, 547, 787
FspBI CTAG 1 cut(s) 515
GluI GCNGC 3 cut(s) 275, 547, 787
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 2 cut(s) 397, 582
HapII CCGG 3 cut(s) 12, 329, 434
Hin1II CATG 6 cut(s) 106, 311, 320, 704, 755, 770
HincII GTYRAC 2 cut(s) 189, 217
HindII GTYRAC 2 cut(s) 189, 217
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 3 cut(s) 6, 71, 811
HpaII CCGG 3 cut(s) 12, 329, 434
HphI GGTGA 3 cut(s) 458, 617, 716
Hpy166II GTNNAC 2 cut(s) 189, 217
Hpy188I TCNGA 2 cut(s) 28, 355
Hpy188III TCNNGA 3 cut(s) 86, 727, 800
Hpy8I GTNNAC 2 cut(s) 189, 217
HpyAV CCTTC 1 cut(s) 814
HpyCH4III ACNGT 4 cut(s) 186, 535, 594, 614
HpyCH4V TGCA 6 cut(s) 311, 501, 530, 755, 766, 789
HpyF3I CTNAG 3 cut(s) 20, 87, 354
Hsp92II CATG 6 cut(s) 106, 311, 320, 704, 755, 770
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 5 cut(s) 325, 566, 625, 646, 706
LmnI GCTCC 3 cut(s) 132, 149, 802
Lsp1109I GCAGC 3 cut(s) 286, 533, 773
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 515
MalI GATC 5 cut(s) 327, 568, 627, 648, 708
MboI GATC 5 cut(s) 325, 566, 625, 646, 706
MboII GAAGA 6 cut(s) 34, 226, 251, 536, 620, 729
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 2 cut(s) 799, 837
MluCI AATT 1 cut(s) 805
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 7 cut(s) 82, 99, 105, 166, 388, 442, 596
Mph1103I ATGCAT 2 cut(s) 313, 757
MroNI GCCGGC 1 cut(s) 11
MseI TTAA 2 cut(s) 426, 850
MspI CCGG 3 cut(s) 12, 329, 434
MspR9I CCNGG 3 cut(s) 435, 464, 564
MvaI CCWGG 2 cut(s) 464, 564
NaeI GCCGGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 435
NdeII GATC 5 cut(s) 325, 566, 625, 646, 706
NgoMIV GCCGGC 1 cut(s) 11
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 6 cut(s) 106, 311, 320, 704, 755, 770
NlaIV GGNNCC 2 cut(s) 798, 828
NsiI ATGCAT 2 cut(s) 313, 757
NspI RCATGY 3 cut(s) 311, 755, 770
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 755
PdiI GCCGGC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 6, 71, 811
PflFI GACNNNGTC 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 3 cut(s) 276, 548, 788
Psp6I CCWGG 2 cut(s) 462, 562
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 2 cut(s) 462, 562
PspN4I GGNNCC 2 cut(s) 798, 828
PspPI GGNCC 3 cut(s) 28, 118, 396
PsuI RGATCY 1 cut(s) 566
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 426, 850
SatI GCNGC 3 cut(s) 275, 547, 787
Sau3AI GATC 5 cut(s) 325, 566, 625, 646, 706
Sau96I GGNCC 3 cut(s) 28, 118, 396
ScrFI CCNGG 3 cut(s) 435, 464, 564
SduI GDGCHC 2 cut(s) 799, 837
SetI ASST 8 cut(s) 58, 250, 324, 453, 607, 623, 682, 825
SfaNI GCATC 1 cut(s) 517
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 2 cut(s) 28, 118
SphI GCATGC 1 cut(s) 755
Sse9I AATT 1 cut(s) 805
SsiI CCGC 4 cut(s) 147, 153, 444, 817
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 3 cut(s) 433, 462, 562
StyI CCWWGG 3 cut(s) 52, 381, 519
TaaI ACNGT 4 cut(s) 186, 535, 594, 614
TaqI TCGA 4 cut(s) 96, 216, 237, 726
TaqII GACCGA 1 cut(s) 206
TasI AATT 1 cut(s) 805
TatI WGTACW 1 cut(s) 64
TfiI GAWTC 3 cut(s) 6, 71, 811
Tru1I TTAA 2 cut(s) 426, 850
Tru9I TTAA 2 cut(s) 426, 850
TscAI CASTG 1 cut(s) 508
TseI GCWGC 3 cut(s) 274, 546, 786
TspDTI ATGAA 2 cut(s) 689, 704
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 1 cut(s) 508
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 2 cut(s) 28, 118
XceI RCATGY 3 cut(s) 311, 755, 770
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 515
Zsp2I ATGCAT 2 cut(s) 313, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.