MD16G1026600.v1.1

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014313
Physical Location & Seq
Reverse (-)
1883424 .. 1886829
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1026600.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGGAATCGTCGGAAATCTCAGTCCTGACCAGCTCCAGACCTTCCGCTCCCAAGGTTATCTTGTCATGGAATCGTTTGCGAGCGCGGAGGACATCGAGGCCATGAGGAGCAGAATGGACCAATTGCTCCAAGACTTTGACTGCTCTTCCACCGCCTCTATTTTCTCCACCAAGAACCAGCAACACACTACTGATAATTACTTCTATGATAGCGTCGACAACATTTCGTTTTTCTTCGAAGAGAAAGCCTTTGGGAGTGATGGAGAACTGCAACAGCCGAAGGAACTTTCGATTAACAAAGTTGGCCATGCGTTACATGAGCTCGATCCAGTATTTAAAAGGTTCACGTATTCTGAGAAAATTTCAGGCTTGTTCTCCTCCTTGGGTTACAAAAGGCCCATTGTCATTCAGTCTATGTACATTTTCAAGCAACCAGGAATCGGAGGTGAGGTAGTGCCACATCAGGATAATTCATTTCTTTATACCGATCCACCAACTTGCACAGGGTTGTGGCTGGCTTTAGAAGATGCAACAATAACGAATGGCTGCCTATGGGCTATTCCTGGATCTCAAAAGAATGGGCTTGTACGACGGTTCATTAGAGGAGAAGAAGGTGTGACCTTTGATCGGCCTTCCCCTGTGTATGATCAAAAAGATTTTATTCCCATCGAAGTGAAAGCTGGGTCTTTAGTTGTTATTCACGGTGATCTTATTCATCAAAGTTTCGAGAATCAGTCCTCGAAATCAAGGCATGCGTACAGCTTGCATGTGGTGGATACCGATGGCTGCAAATGGTCTCAAGATAATTGGATCAGGAGAAAAGTGGAGCCAGAGCCTCTCTATGTATCTTCTTTGACCTAA

Protein Analysis

287

Amino Acids

32.31

Weight (kDa)

5.18

Isoelectric Point (pI)

57.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 15 - 252 3e-54 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01490 AT2G01490
fragaria_vesca FvH4_4g32640
malus_domestica MD13G1023900.v1.1 MD16G1026600.v1.1
prunus_persica Prupe.1G326800_v2.0.a1
pyrus_communis pycom16g02240
rosa_chinensis RchiOBHm_Chr4g0441521
rosa_laevigata RLG00000006088
rosa_roxburghii Rroxscaffold_5G00382370 Rroxscaffold_7G00185860
rosa_rugosa Rorug04G0334400 Rorug06G0150600
rosa_samantha Rh4BG399900 Rh4CG414900
rosa_wichuraiana Rw4G033320 Rw6G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 1 cut(s) 216
AccII CGCG 1 cut(s) 86
AciI CCGC 3 cut(s) 46, 86, 153
AclWI GGATC 4 cut(s) 320, 482, 574, 818
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 360
AfaI GTAC 3 cut(s) 419, 588, 758
AfiI CCNNNNNNNGG 2 cut(s) 440, 627
AgsI TTSAA 1 cut(s) 427
AjnI CCWGG 2 cut(s) 433, 562
AluBI AGCT 4 cut(s) 34, 322, 680, 762
AluI AGCT 4 cut(s) 34, 322, 680, 762
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 801
AlwI GGATC 4 cut(s) 320, 482, 574, 818
AoxI GGCC 4 cut(s) 99, 304, 395, 629
ApeKI GCWGC 2 cut(s) 546, 786
ApoI RAATTY 1 cut(s) 360
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 118, 396
AsuHPI GGTGA 2 cut(s) 458, 716
AsuII TTCGAA 1 cut(s) 237
AvaII GGWCC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 306
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 533, 773
BccI CCATC 3 cut(s) 254, 674, 776
BciT130I CCWGG 2 cut(s) 435, 564
BciVI GTATCC 1 cut(s) 769
BclI TGATCA 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 801
BfuI GTATCC 1 cut(s) 769
BisI GCNGC 2 cut(s) 547, 787
BlsI GCNGC 2 cut(s) 548, 788
Bme1390I CCNGG 2 cut(s) 435, 564
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 396
BmiI GGNNCC 1 cut(s) 828
BmrFI CCNGG 2 cut(s) 435, 564
BmsI GCATC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 20
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 783
BsaAI YACGTR 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 801
BsaJI CCNNGG 2 cut(s) 52, 381
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 627
Bse1I ACTGG 1 cut(s) 329
BseBI CCWGG 2 cut(s) 435, 564
BseDI CCNNGG 2 cut(s) 52, 381
BseLI CCNNNNNNNGG 2 cut(s) 440, 627
BseMII CTCAG 2 cut(s) 34, 345
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 3 cut(s) 121, 367, 618
BseXI GCAGC 2 cut(s) 533, 773
BseYI CCCAGC 1 cut(s) 680
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 4 cut(s) 101, 306, 397, 631
BsiHKAI GWGCWC 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 440, 627
BsmAI GTCTC 1 cut(s) 801
BsnI GGCC 4 cut(s) 101, 306, 397, 631
Bso31I GGTCTC 1 cut(s) 801
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 324
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
BspACI CCGC 3 cut(s) 46, 86, 153
BspANI GGCC 4 cut(s) 101, 306, 397, 631
BspCNI CTCAG 2 cut(s) 33, 346
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 828
BspPI GGATC 4 cut(s) 320, 482, 574, 818
BspQI GCTCTTC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 801
BsrBI CCGCTC 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 52, 381
BssMI GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
BssT1I CCWWGG 2 cut(s) 52, 381
Bst2UI CCWGG 2 cut(s) 435, 564
Bst4CI ACNGT 2 cut(s) 594, 704
Bst6I CTCTTC 2 cut(s) 151, 234
BstAUI TGTACA 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 348
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 82, 516, 753, 764
BstDEI CTNAG 2 cut(s) 20, 354
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 7 cut(s) 328, 490, 569, 628, 649, 709, 813
BstMAI GTCTC 1 cut(s) 801
BstMBI GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
BstNI CCWGG 2 cut(s) 435, 564
BstNSI RCATGY 2 cut(s) 755, 770
BstSCI CCNGG 2 cut(s) 433, 562
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 2 cut(s) 533, 773
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 4 cut(s) 101, 306, 397, 631
Cac8I GCNNGC 4 cut(s) 82, 516, 753, 764
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 118, 396
CseI GACGC 1 cut(s) 202
Csp6I GTAC 3 cut(s) 418, 587, 757
CviAII CATG 6 cut(s) 67, 103, 308, 317, 752, 767
CviQI GTAC 3 cut(s) 418, 587, 757
DdeI CTNAG 2 cut(s) 20, 354
DpnI GATC 7 cut(s) 327, 489, 568, 627, 648, 708, 812
DpnII GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 304
Eam1104I CTCTTC 2 cut(s) 151, 234
EarI CTCTTC 2 cut(s) 151, 234
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 52, 381
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 801
Eco47I GGWCC 1 cut(s) 118
Eco53kI GAGCTC 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 433, 562
EcoT14I CCWWGG 2 cut(s) 52, 381
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 52, 381
FaeI CATG 6 cut(s) 70, 106, 311, 320, 755, 770
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FatI CATG 6 cut(s) 66, 102, 307, 316, 751, 766
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 547, 787
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 2 cut(s) 547, 787
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 547, 787
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 4 cut(s) 101, 306, 397, 631
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 86
Hin1II CATG 6 cut(s) 70, 106, 311, 320, 755, 770
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 4 cut(s) 6, 71, 438, 730
HphI GGTGA 2 cut(s) 458, 716
Hpy166II GTNNAC 2 cut(s) 217, 345
Hpy188I TCNGA 3 cut(s) 14, 355, 443
Hpy188III TCNNGA 6 cut(s) 26, 37, 464, 727, 800, 814
Hpy8I GTNNAC 2 cut(s) 217, 345
Hpy99I CGWCG 3 cut(s) 14, 218, 594
HpyAV CCTTC 4 cut(s) 52, 274, 605, 642
HpyCH4III ACNGT 2 cut(s) 594, 704
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 5 cut(s) 271, 501, 530, 766, 789
HpyF3I CTNAG 2 cut(s) 20, 354
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 6 cut(s) 70, 106, 311, 320, 755, 770
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 5 cut(s) 39, 53, 108, 132, 826
Lsp1109I GCAGC 2 cut(s) 533, 773
LweI GCATC 1 cut(s) 517
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 3 cut(s) 312, 386, 616
MalI GATC 7 cut(s) 327, 489, 568, 627, 648, 708, 812
MbiI CCGCTC 1 cut(s) 48
MboI GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
MboII GAAGA 6 cut(s) 138, 226, 251, 536, 620, 840
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 1 cut(s) 324
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 5 cut(s) 122, 196, 360, 469, 805
MluNI TGGCCA 1 cut(s) 306
Mox20I TGGCCA 1 cut(s) 306
MscI TGGCCA 1 cut(s) 306
MseI TTAA 2 cut(s) 294, 336
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 306
MspR9I CCNGG 2 cut(s) 435, 564
MunI CAATTG 1 cut(s) 122
MvaI CCWGG 2 cut(s) 435, 564
MvnI CGCG 1 cut(s) 86
NdeII GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
NlaIII CATG 6 cut(s) 70, 106, 311, 320, 755, 770
NlaIV GGNNCC 1 cut(s) 828
NmuCI GTSAC 1 cut(s) 616
NspI RCATGY 2 cut(s) 755, 770
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 151
PfeI GAWTC 4 cut(s) 6, 71, 438, 730
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 2 cut(s) 548, 788
Ppu21I YACGTR 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 433, 562
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 2 cut(s) 433, 562
PspN4I GGNNCC 1 cut(s) 828
PspPI GGNCC 2 cut(s) 118, 396
PsuI RGATCY 1 cut(s) 566
RsaI GTAC 3 cut(s) 419, 588, 758
RsaNI GTAC 3 cut(s) 418, 587, 757
RseI CAYNNNNRTG 1 cut(s) 671
SacI GAGCTC 1 cut(s) 324
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 294, 336
SatI GCNGC 2 cut(s) 547, 787
Sau3AI GATC 7 cut(s) 325, 487, 566, 625, 646, 706, 810
Sau96I GGNCC 2 cut(s) 118, 396
ScrFI CCNGG 2 cut(s) 435, 564
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 1 cut(s) 517
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
SphI GCATGC 1 cut(s) 755
Sse9I AATT 5 cut(s) 122, 196, 360, 469, 805
SsiI CCGC 3 cut(s) 46, 86, 153
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 433, 562
StyI CCWWGG 2 cut(s) 52, 381
TaaI ACNGT 2 cut(s) 594, 704
TaiI ACGT 1 cut(s) 350
TaqI TCGA 8 cut(s) 96, 216, 237, 290, 324, 669, 726, 740
TasI AATT 5 cut(s) 122, 196, 360, 469, 805
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 4 cut(s) 6, 71, 438, 730
Tru1I TTAA 2 cut(s) 294, 336
Tru9I TTAA 2 cut(s) 294, 336
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 2 cut(s) 546, 786
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 3 cut(s) 462, 586, 704
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 360
XceI RCATGY 2 cut(s) 755, 770
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.