Rw6G022810

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014313
Physical Location & Seq
Forward (+)
44861090 .. 44872633
11544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022810.1

Sequence Viewer

Length: 633 bp
ATGTTGCAAAGGGACTTTAAGAATGATCAATTAAATTACTTTCAATACCTCCCAAACAAACCTTTCCAACTTCCAACCAAAGTCAAAGCTATGGGACAGCGAGGCAACTGGATCATTTCTTGCTCGCACCTTTCCGGGTACTTTAGCCTAAAAGAGGTCCAGCTCAGCTCCATATACTTTTTGCACCAAGATGGTTCCAAGAAGCAGCCTTACTATCAACCGAGTATTGGAGGTGAGGTAGTGCCACATCAGGATAACTCATTTCTTTATACCGAAGCACAAACTTGCACAGGTATGTGGCTAGCCTTGGAAGATGCAACAGTAGTAAATGGCTGCCTTTGGGCTATTCCTGGATCTCAAAAGATTTTCCTCTTATCTGTCTTTATCTGGAATGCAAATGGCCTTGTTAGATGGTTTATTAGAGGTGAAGACGGTGTGACCTTTGATCGGCCTTCCCCTGCATATGATCCAAAAGATTTTATGCCTATTGAAGTCAAAGTTGGGTCTTTAATTGTTATTCGTGGTGATCTTGTTCATCAAAGTTTTGAGAACCAGTCTTTGAAGTCAAGGCATGCGTATAGCTTGCATGTGGTGGATACAGATGGCTGCAAATGGGCTCTAGATAATTGGTAA

Protein Analysis

210

Amino Acids

24.03

Weight (kDa)

6.58

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 69 - 192 5.2e-28 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01490 AT2G01490
fragaria_vesca FvH4_4g32640
malus_domestica MD13G1023900.v1.1 MD16G1026600.v1.1
prunus_persica Prupe.1G326800_v2.0.a1
pyrus_communis pycom16g02240
rosa_chinensis RchiOBHm_Chr4g0441521
rosa_laevigata RLG00000006088
rosa_roxburghii Rroxscaffold_5G00382370 Rroxscaffold_7G00185860
rosa_rugosa Rorug04G0334400 Rorug06G0150600
rosa_samantha Rh4BG399900 Rh4CG414900
rosa_wichuraiana Rw4G033320 Rw6G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 119, 361, 461
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 3 cut(s) 154, 227, 447
AgsI TTSAA 3 cut(s) 44, 491, 562
AjnI CCWGG 1 cut(s) 349
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 4 cut(s) 89, 163, 168, 582
AluI AGCT 4 cut(s) 89, 163, 168, 582
AlwI GGATC 3 cut(s) 119, 361, 461
AoxI GGCC 2 cut(s) 400, 449
ApeKI GCWGC 3 cut(s) 205, 333, 606
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 3 cut(s) 245, 437, 536
AsuNHI GCTAGC 1 cut(s) 301
AvaII GGWCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 619
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
BbsI GAAGAC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 217, 320, 593
BccI CCATC 3 cut(s) 185, 405, 596
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 589
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 136
BfaI CTAG 2 cut(s) 302, 620
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 3 cut(s) 206, 334, 607
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 3 cut(s) 207, 335, 608
Bme1390I CCNGG 2 cut(s) 136, 351
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 136, 351
BmsI GCATC 1 cut(s) 304
BmtI GCTAGC 1 cut(s) 305
BpiI GAAGAC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 227, 447
Bse1I ACTGG 2 cut(s) 113, 553
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 154, 227, 447
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 2 cut(s) 113, 553
BseXI GCAGC 3 cut(s) 217, 320, 593
BshFI GGCC 2 cut(s) 402, 451
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 2 cut(s) 26, 108
BslI CCNNNNNNNGG 3 cut(s) 154, 227, 447
BsmFI GGGAC 2 cut(s) 26, 108
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 402, 451
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 6 cut(s) 25, 111, 353, 445, 466, 526
Bsp1720I GCTNAGC 1 cut(s) 164
BspANI GGCC 2 cut(s) 402, 451
BspCNI CTCAG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 305
BspPI GGATC 3 cut(s) 119, 361, 461
BsrI ACTGG 2 cut(s) 113, 553
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 6 cut(s) 25, 111, 353, 445, 466, 526
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 322, 434
BstC8I GCNNGC 4 cut(s) 125, 303, 573, 584
BstDEI CTNAG 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 152
BstKTI GATC 6 cut(s) 28, 114, 356, 448, 469, 529
BstMBI GATC 6 cut(s) 25, 111, 353, 445, 466, 526
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 2 cut(s) 575, 590
BstSCI CCNGG 2 cut(s) 134, 349
BstV1I GCAGC 3 cut(s) 217, 320, 593
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 2 cut(s) 402, 451
Cac8I GCNNGC 4 cut(s) 125, 303, 573, 584
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 572, 587
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 164
DpnI GATC 6 cut(s) 27, 113, 355, 447, 468, 528
DpnII GATC 6 cut(s) 25, 111, 353, 445, 466, 526
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 619
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 575, 590
FaqI GGGAC 2 cut(s) 26, 108
FatI CATG 2 cut(s) 571, 586
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 206, 334, 607
FriOI GRGCYC 1 cut(s) 619
Fsp4HI GCNGC 3 cut(s) 206, 334, 607
FspBI CTAG 2 cut(s) 302, 620
GluI GCNGC 3 cut(s) 206, 334, 607
HaeIII GGCC 2 cut(s) 402, 451
HapII CCGG 1 cut(s) 135
Hin1II CATG 2 cut(s) 575, 590
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 245, 437, 536
Hpy188III TCNNGA 3 cut(s) 251, 388, 620
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 2 cut(s) 322, 434
HpyCH4V TGCA 8 cut(s) 7, 184, 288, 317, 395, 461, 586, 609
HpyF3I CTNAG 1 cut(s) 164
Hsp92II CATG 2 cut(s) 575, 590
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 6 cut(s) 25, 111, 353, 445, 466, 526
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 3 cut(s) 217, 320, 593
LweI GCATC 1 cut(s) 304
MaeI CTAG 2 cut(s) 302, 620
MaeIII GTNAC 1 cut(s) 436
MalI GATC 6 cut(s) 27, 113, 355, 447, 468, 528
MboI GATC 6 cut(s) 25, 111, 353, 445, 466, 526
MboII GAAGA 2 cut(s) 323, 440
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 4 cut(s) 29, 34, 510, 625
MmeI TCCRAC 2 cut(s) 91, 98
MnlI CCTC 7 cut(s) 59, 95, 148, 224, 229, 380, 416
MseI TTAA 3 cut(s) 18, 32, 509
MslI CAYNNNNRTG 2 cut(s) 189, 293
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 2 cut(s) 136, 351
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 1 cut(s) 351
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 463
NdeII GATC 6 cut(s) 25, 111, 353, 445, 466, 526
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 2 cut(s) 575, 590
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 436
NspI RCATGY 2 cut(s) 575, 590
PaeI GCATGC 1 cut(s) 575
PctI GAATGC 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 3 cut(s) 207, 335, 608
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 2 cut(s) 189, 293
SaqAI TTAA 3 cut(s) 18, 32, 509
SatI GCNGC 3 cut(s) 206, 334, 607
Sau3AI GATC 6 cut(s) 25, 111, 353, 445, 466, 526
Sau96I GGNCC 1 cut(s) 157
ScrFI CCNGG 2 cut(s) 136, 351
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 1 cut(s) 304
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 2 cut(s) 189, 293
SphI GCATGC 1 cut(s) 575
Sse9I AATT 4 cut(s) 29, 34, 510, 625
SspMI CTAG 2 cut(s) 302, 620
StyD4I CCNGG 2 cut(s) 134, 349
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 322, 434
TasI AATT 4 cut(s) 29, 34, 510, 625
Tru1I TTAA 3 cut(s) 18, 32, 509
Tru9I TTAA 3 cut(s) 18, 32, 509
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 3 cut(s) 205, 333, 606
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 157
XagI CCTNNNNNAGG 1 cut(s) 152
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 2 cut(s) 575, 590
XspI CTAG 2 cut(s) 302, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.