Rroxscaffold_7G00185860

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014313
Physical Location & Seq
Reverse (-)
25342213 .. 25343777
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185860.1

Sequence Viewer

Length: 393 bp
ATGCTCGATATTCCATGGCTAAATGATTCTTCGTCTTCCAATTTTGATAAGTTGCTTAAACCATATGAAGGGTCTGATTTGCAACCGGGTATTGGAGGCAAGGTGGTGCCACATCAGGATAACTCATTTCTTTATACCGAACCACAAACTTGCACAGATGGCCTTGTTAGACGGTTTATTAGAGGAGAAGACGGTGTGACCTTTGATCGGTCTTCCCCTGCGTATGATCCAAAAGATTTTGTGCCTATTGAAGTCAAAGCTGGATCATTAATTGTTATTCATGGTGATCTTATTCATCAAAGTTCTGAGAGCCAGTCTTTGAAGTCAAGGCATGCGTATAGCTTGCATGTGGTGGATACAGATGGCTGCAAATGGGCTCCAGATAATTGGTAA

Protein Analysis

130

Amino Acids

14.49

Weight (kDa)

5.01

Isoelectric Point (pI)

58.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 53 - 112 2.6e-09 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01490 AT2G01490
fragaria_vesca FvH4_4g32640
malus_domestica MD13G1023900.v1.1 MD16G1026600.v1.1
prunus_persica Prupe.1G326800_v2.0.a1
pyrus_communis pycom16g02240
rosa_chinensis RchiOBHm_Chr4g0441521
rosa_laevigata RLG00000006088
rosa_roxburghii Rroxscaffold_5G00382370 Rroxscaffold_7G00185860
rosa_rugosa Rorug04G0334400 Rorug06G0150600
rosa_samantha Rh4BG399900 Rh4CG414900
rosa_wichuraiana Rw4G033320 Rw6G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 106
AclWI GGATC 2 cut(s) 221, 271
AfiI CCNNNNNNNGG 3 cut(s) 68, 92, 207
AgsI TTSAA 2 cut(s) 251, 322
AluBI AGCT 2 cut(s) 260, 342
AluI AGCT 2 cut(s) 260, 342
AlwI GGATC 2 cut(s) 221, 271
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 366
AseI ATTAAT 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 296
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 379
BbsI GAAGAC 3 cut(s) 27, 195, 204
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 152, 356
BciVI GTATCC 1 cut(s) 349
BcnI CCSGG 1 cut(s) 87
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 108, 378
BmrFI CCNGG 1 cut(s) 87
BpiI GAAGAC 3 cut(s) 27, 195, 204
BpmI CTGGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 92, 207
Bse1I ACTGG 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 68, 92, 207
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 353
BshFI GGCC 1 cut(s) 162
BshNI GGYRCC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 86
BslI CCNNNNNNNGG 3 cut(s) 68, 92, 207
BsnI GGCC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 4 cut(s) 205, 226, 263, 286
Bsp19I CCATGG 1 cut(s) 14
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 108, 378
BspPI GGATC 2 cut(s) 221, 271
BspT107I GGYRCC 1 cut(s) 106
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 4 cut(s) 205, 226, 263, 286
BssT1I CCWWGG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 174, 194
BstC8I GCNNGC 2 cut(s) 333, 344
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 14
BstKTI GATC 4 cut(s) 208, 229, 266, 289
BstMBI GATC 4 cut(s) 205, 226, 263, 286
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNSI RCATGY 2 cut(s) 335, 350
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 353
BstV2I GAAGAC 3 cut(s) 27, 195, 204
BstXI CCANNNNNNTGG 1 cut(s) 387
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 14
Cac8I GCNNGC 2 cut(s) 333, 344
CviAII CATG 4 cut(s) 15, 281, 332, 347
CviJI RGCY 7 cut(s) 19, 162, 260, 312, 342, 366, 377
CviKI_1 RGCY 7 cut(s) 19, 162, 260, 312, 342, 366, 377
DdeI CTNAG 1 cut(s) 306
DpnI GATC 4 cut(s) 207, 228, 265, 288
DpnII GATC 4 cut(s) 205, 226, 263, 286
Eco130I CCWWGG 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 14
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 4 cut(s) 18, 284, 335, 350
FaiI YATR 9 cut(s) 16, 64, 66, 135, 225, 282, 333, 339, 348
FatI CATG 4 cut(s) 14, 280, 331, 346
FauNDI CATATG 1 cut(s) 64
Fnu4HI GCNGC 1 cut(s) 367
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 367
GluI GCNGC 1 cut(s) 367
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 86
Hin1II CATG 4 cut(s) 18, 284, 335, 350
HinfI GANTC 1 cut(s) 26
HpaII CCGG 1 cut(s) 86
HphI GGTGA 1 cut(s) 296
Hpy188I TCNGA 2 cut(s) 76, 307
Hpy188III TCNNGA 2 cut(s) 116, 380
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 174, 194
HpyCH4V TGCA 4 cut(s) 82, 153, 346, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 4 cut(s) 18, 284, 335, 350
Kzo9I GATC 4 cut(s) 205, 226, 263, 286
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 5 cut(s) 99, 101, 231, 246, 326
Lsp1109I GCAGC 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 196
MalI GATC 4 cut(s) 207, 228, 265, 288
MboI GATC 4 cut(s) 205, 226, 263, 286
MboII GAAGA 4 cut(s) 21, 27, 200, 204
MhlI GDGCHC 1 cut(s) 379
MluCI AATT 3 cut(s) 40, 270, 385
MnlI CCTC 2 cut(s) 89, 176
MseI TTAA 2 cut(s) 57, 269
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 159
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 14
NdeI CATATG 1 cut(s) 64
NdeII GATC 4 cut(s) 205, 226, 263, 286
NlaIII CATG 4 cut(s) 18, 284, 335, 350
NlaIV GGNNCC 2 cut(s) 108, 378
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 2 cut(s) 335, 350
PaeI GCATGC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 1 cut(s) 368
PshBI ATTAAT 1 cut(s) 269
PspN4I GGNNCC 2 cut(s) 108, 378
SaqAI TTAA 2 cut(s) 57, 269
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 4 cut(s) 205, 226, 263, 286
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 379
SetI ASST 4 cut(s) 105, 203, 262, 344
SphI GCATGC 1 cut(s) 335
Sse9I AATT 3 cut(s) 40, 270, 385
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 2 cut(s) 174, 194
TaqI TCGA 1 cut(s) 6
TaqII GACCGA 1 cut(s) 198
TasI AATT 3 cut(s) 40, 270, 385
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 57, 269
Tru9I TTAA 2 cut(s) 57, 269
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 366
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 81, 269, 284
VspI ATTAAT 1 cut(s) 269
XceI RCATGY 2 cut(s) 335, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.