FvH4_4g32820

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008691
Physical Location & Seq
Reverse (-)
31301694 .. 31304296
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32820.t1

Sequence Viewer

Length: 1860 bp
ATGGCTTCCACTTCAACTTCTTCAATATCTTTGCAAACTAGTGCACAGAAACAAAGCTTCCCTTCAAATTCAGCAGCCACAACATTCCACACAAACCTTAGTTTTCCATATAATCCGTTGCCATTTACCAAATTTGTATGTGTTAGATCTTCTAGCACTTCCAAGTACTACAATGAAGTTGTGGTGGATGAAGAGATGGACAGAATACGGAGGCTTCAAAACGGTTCAGATGTTCGTGGGGTGGCCTTGGAAGGTGAGAAGGGAAGGTCAGTCGACCTTACGCCTCCGGCTGTGGAGGCAATTGCAGAGAGCTTTGGTGAGTGGGTTATCAATGGCTTGGAGAAGCAGAGAGGACAGCCTGTGGAGAATGTTAAGGTGTCCCTTGGCAGAGACCCTAGAATATCAGGACCATCTTTGAGTGTTGCTGTCTTTTCAGGTCTAGCTCGTGCCGGTTGTTTGGTGTTTGATATGGGACTTGCAACCACTCCTGCTTGCTTCATGAGTACAATTTTGCCTCCATTTTCCTATGACGCTTCTATCATGATGACTGCATCTCACTTGCCCTATACTCGTAACGGTTTGAAATTCTTCACCAAGAGAGGGGGACTGACCTCGCCGGAAGTGGAGGAAATATGTGACAAAGCGGCGAGAAAGTATGCTAATAGGCTCACCAAAGTGTCAAGTGTTCTTCGCATACCACCGTCAAGGGTTGATTTCATGAGCTCTTACTCGGAGCACCTCCGGAACATTATCAAGGAGAGAGTGAATCATCCACTACATTATGACACTCCACTACAAGGATTTCAGATAATAGTCAATGCTGGGAATGGATCAGGAGGCTTCTTCACATGGGATGTGTTAGACAAGCTAGGAGCAGACACCTTTGGCTCTCTACACCTCAAACCAGATGGGATGTTTCCAAACCACATTCCCAACCCAGAAGACAAAGTTGCCATGACACTTACAAGAGCTGCAGTGCTAGAAAACTCTGCTGATCTTGGAATCGTATTCGACACTGATGTGGACCGCAGTGGTGTTGTAGATAACAAAGGCAATCCCATTAATGGGGACAAGCTTATTGCTTTGATTTCAGCTATTGTATTGAAGGAACACCCTGGAACCACCATTGTGACCGATGCTCGAACAAGTATGGCACTTACTAGATTCATCACCAACAGAGGAGGTCACCATTGCTTATATCGCGTTGGTTATAGAAATGTGATTGAAAAGGGAATCCAGCTTAATAAGGATGGCATTGAAACTCATCTTATGATGGAAACTTCAGGACATGGTGCTCTGAGAGAGAACTTTTTCCTTGATGATGGGGCTTACATGGTGGTGAAGATCATCATTGAGATGGTACAAATGAAGCTTGCTGGATCAGATGAAGGGATAGGAAGTATCATTAACGATCTTGAAGAACCAGAAGAATCTGTAGAGCTAAGGATGAATGTAGTCTCTGAGCCTAGATATGCAAAAGAAAAGGCCATCGAGGCAATTGAAACGTTTCGACAGTACGTTGAGGAGGGAAGAATTGAAGGATGGGAATTAGATACATGTGGCGATTGTTGGGTCAGTGAAGGCTGTCTAGTAGACTCAAATGAAACCTCGGCTGCAGCAGTTGATGCTCACATGTACAGGGTAAAAGTATCAGATGAGGTGCATGGACAGCATGGTTGGGTACACATTCGGCAAAGCATTCACAACCCTAATATTGCAGTGAATATGCAATCATCTGTGCCTGGCTCTTGCCAAACGATGACCAGAGTTCTTCGAGATAGATTTCTCATAGCTAGTGAAATGGACAGAGTCCTTGACATGACTGAGATTGACAAGTATGCCAAAAGTGGATTTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004615 GO:0005975 GO:0008150 GO:0008152 GO:0016853 GO:0016866 GO:0016868 GO:0044238 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

620

Amino Acids

68.29

Weight (kDa)

5.64

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 72 - 217 9.9e-24 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 243 - 351 9.3e-21 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 355 - 455 3.6e-11 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 273, 1593
AccII CGCG 1 cut(s) 1203
AccIII TCCGGA 1 cut(s) 741
AciI CCGC 2 cut(s) 644, 1027
AclI AACGTT 1 cut(s) 1505
AclWI GGATC 2 cut(s) 838, 1387
AcsI RAATTY 3 cut(s) 67, 131, 584
AcuI CTGAAG 1 cut(s) 1266
AfaI GTAC 6 cut(s) 167, 505, 1362, 1517, 1637, 1683
AfiI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
AflIII ACRYGT 2 cut(s) 1556, 1632
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 2 cut(s) 1114, 1741
AleI CACNNNNGTG 3 cut(s) 674, 1019, 1127
Alw21I GWGCWC 4 cut(s) 46, 725, 738, 1297
Alw26I GTCTC 2 cut(s) 384, 1462
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 2 cut(s) 838, 1387
Aor13HI TCCGGA 1 cut(s) 741
AoxI GGCC 2 cut(s) 243, 1485
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 4 cut(s) 74, 971, 1613, 1616
ApoI RAATTY 3 cut(s) 67, 131, 584
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 3 cut(s) 587, 1310, 1506
AspS9I GGNCC 2 cut(s) 407, 1024
AsuHPI GGTGA 7 cut(s) 266, 329, 583, 661, 1162, 1178, 1351
AvaII GGWCC 2 cut(s) 407, 1024
BaeGI GKGCMC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 725
BauI CACGAG 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 948
Bbv12I GWGCWC 4 cut(s) 46, 725, 738, 1297
BbvI GCAGC 4 cut(s) 86, 958, 1600, 1628
BccI CCATC 9 cut(s) 190, 418, 902, 1244, 1267, 1316, 1351, 1496, 1536
BciT130I CCWGG 2 cut(s) 1116, 1743
BcoDI GTCTC 2 cut(s) 384, 1462
BcuI ACTAGT 1 cut(s) 38
BfmI CTRYAG 4 cut(s) 972, 1434, 1614, 1856
BglI GCCNNNNNGGC 1 cut(s) 1493
BglII AGATCT 1 cut(s) 146
BisI GCNGC 5 cut(s) 75, 645, 972, 1614, 1617
BlsI GCNGC 5 cut(s) 76, 646, 973, 1615, 1618
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 1116, 1743
Bme18I GGWCC 2 cut(s) 407, 1024
BmgT120I GGNCC 2 cut(s) 407, 1024
BmiI GGNNCC 1 cut(s) 1120
BmrFI CCNGG 2 cut(s) 1116, 1743
BmsI GCATC 3 cut(s) 560, 1126, 1615
BpiI GAAGAC 1 cut(s) 948
Bpu10I CCTNAGC 1 cut(s) 1442
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 4 cut(s) 246, 382, 1114, 1608
BsaWI WCCGGW 1 cut(s) 741
Bsc4I CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
Bse118I RCCGGY 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 1189
BseAI TCCGGA 1 cut(s) 741
BseBI CCWGG 2 cut(s) 1116, 1743
BseDI CCNNGG 4 cut(s) 246, 382, 1114, 1608
BseGI GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BseLI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
BseMI GCAATG 1 cut(s) 1189
BseMII CTCAG 3 cut(s) 1289, 1452, 1815
BseRI GAGGAG 2 cut(s) 1194, 1538
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 4 cut(s) 86, 958, 1600, 1628
BseYI CCCAGC 1 cut(s) 821
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 2 cut(s) 245, 1487
BsiHKAI GWGCWC 4 cut(s) 46, 725, 738, 1297
BsiSI CCGG 4 cut(s) 287, 450, 617, 742
BslFI GGGAC 4 cut(s) 364, 486, 618, 1082
BslI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
BsmAI GTCTC 2 cut(s) 384, 1462
BsmFI GGGAC 4 cut(s) 364, 486, 618, 1082
BsmI GAATGC 1 cut(s) 1698
BsnI GGCC 2 cut(s) 245, 1487
Bso31I GGTCTC 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 46, 725, 738, 1297
Bsp13I TCCGGA 1 cut(s) 741
Bsp1407I TGTACA 1 cut(s) 1635
Bsp143I GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
BspACI CCGC 2 cut(s) 644, 1027
BspANI GGCC 2 cut(s) 245, 1487
BspCNI CTCAG 3 cut(s) 1290, 1453, 1816
BspEI TCCGGA 1 cut(s) 741
BspFNI CGCG 1 cut(s) 1203
BspHI TCATGA 3 cut(s) 498, 540, 717
BspLI GGNNCC 1 cut(s) 1120
BspMAI CTGCAG 2 cut(s) 976, 1618
BspPI GGATC 2 cut(s) 838, 1387
BspTNI GGTCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 1189
BsrFI RCCGGY 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 1635
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 4 cut(s) 246, 382, 1114, 1608
BssMI GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 2 cut(s) 246, 382
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 2 cut(s) 1116, 1743
Bst4CI ACNGT 4 cut(s) 224, 578, 702, 1515
Bst6I CTCTTC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstAUI TGTACA 1 cut(s) 1635
BstC8I GCNNGC 2 cut(s) 493, 1374
BstDEI CTNAG 5 cut(s) 98, 1298, 1442, 1461, 1824
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BstFNI CGCG 1 cut(s) 1203
BstKTI GATC 6 cut(s) 149, 833, 997, 1347, 1382, 1414
BstMAI GTCTC 2 cut(s) 384, 1462
BstMBI GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
BstMWI GCNNNNNNNGC 5 cut(s) 296, 1200, 1493, 1625, 1669
BstNI CCWGG 2 cut(s) 1116, 1743
BstNSI RCATGY 2 cut(s) 1560, 1636
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 2 cut(s) 1114, 1741
BstSFI CTRYAG 4 cut(s) 972, 1434, 1614, 1856
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 4 cut(s) 86, 958, 1600, 1628
BstV2I GAAGAC 1 cut(s) 948
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 245, 1487
BtsCI GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BtsI GCAGTG 3 cut(s) 981, 1036, 1725
BtsIMutI CAGTG 5 cut(s) 981, 1014, 1036, 1582, 1725
Cac8I GCNNGC 2 cut(s) 493, 1374
CciI TCATGA 3 cut(s) 498, 540, 717
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 407, 1024
CseI GACGC 1 cut(s) 539
Csp6I GTAC 6 cut(s) 166, 504, 1361, 1516, 1636, 1682
CviQI GTAC 6 cut(s) 166, 504, 1361, 1516, 1636, 1682
DdeI CTNAG 5 cut(s) 98, 1298, 1442, 1461, 1824
DpnI GATC 6 cut(s) 148, 832, 996, 1346, 1381, 1413
DpnII GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 2 cut(s) 246, 382
Eco24I GRGCYC 1 cut(s) 725
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 407, 1024
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 1266
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 1184
EcoRII CCWGG 2 cut(s) 1114, 1741
EcoT14I CCWWGG 2 cut(s) 246, 382
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 2 cut(s) 246, 382
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FaqI GGGAC 4 cut(s) 364, 486, 618, 1082
FblI GTMKAC 2 cut(s) 273, 1593
Fnu4HI GCNGC 5 cut(s) 75, 645, 972, 1614, 1617
FokI GGATG 7 cut(s) 200, 756, 866, 925, 1262, 1459, 1554
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 5 cut(s) 75, 645, 972, 1614, 1617
GluI GCNGC 5 cut(s) 75, 645, 972, 1614, 1617
GsaI CCCAGC 1 cut(s) 825
HaeIII GGCC 2 cut(s) 245, 1487
HapII CCGG 4 cut(s) 287, 450, 617, 742
HgaI GACGC 1 cut(s) 539
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 3 cut(s) 55, 1073, 1370
HinfI GANTC 7 cut(s) 766, 1002, 1164, 1233, 1430, 1595, 1809
HpaII CCGG 4 cut(s) 287, 450, 617, 742
HphI GGTGA 7 cut(s) 266, 329, 583, 661, 1162, 1178, 1351
Hpy166II GTNNAC 5 cut(s) 44, 274, 1024, 1594, 1684
Hpy188I TCNGA 7 cut(s) 229, 733, 807, 1299, 1384, 1462, 1654
Hpy188III TCNNGA 9 cut(s) 405, 499, 541, 718, 742, 834, 1284, 1415, 1775
Hpy8I GTNNAC 5 cut(s) 44, 274, 1024, 1594, 1684
HpyAV CCTTC 8 cut(s) 72, 245, 253, 258, 1099, 1382, 1532, 1574
HpyCH4III ACNGT 4 cut(s) 224, 578, 702, 1515
HpyCH4IV ACGT 2 cut(s) 1505, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 1200, 1493, 1625, 1669
HpyF3I CTNAG 5 cut(s) 98, 1298, 1442, 1461, 1824
HpySE526I ACGT 2 cut(s) 1505, 1518
Kpn2I TCCGGA 1 cut(s) 741
Kzo9I GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
LmnI GCTCC 2 cut(s) 733, 872
Lsp1109I GCAGC 4 cut(s) 86, 958, 1600, 1628
LweI GCATC 3 cut(s) 560, 1126, 1615
MaeII ACGT 2 cut(s) 1505, 1518
MaeIII GTNAC 4 cut(s) 572, 635, 1129, 1184
MalI GATC 6 cut(s) 148, 832, 996, 1346, 1381, 1413
MboI GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
MfeI CAATTG 2 cut(s) 300, 1497
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 4 cut(s) 46, 725, 738, 1297
MluCI AATT 8 cut(s) 67, 131, 300, 507, 584, 1497, 1533, 1547
MlyI GAGTC 2 cut(s) 1589, 1818
MroI TCCGGA 1 cut(s) 741
MroXI GAANNNNTTC 3 cut(s) 587, 1310, 1506
MseI TTAA 4 cut(s) 372, 1062, 1242, 1407
MslI CAYNNNNRTG 5 cut(s) 674, 1019, 1127, 1337, 1355
MspI CCGG 4 cut(s) 287, 450, 617, 742
MspR9I CCNGG 2 cut(s) 1116, 1743
MunI CAATTG 2 cut(s) 300, 1497
Mva1269I GAATGC 1 cut(s) 1698
MvaI CCWGG 2 cut(s) 1116, 1743
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 5 cut(s) 296, 1200, 1493, 1625, 1669
NdeII GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
NlaIV GGNNCC 1 cut(s) 1120
NmeAIII GCCGAG 1 cut(s) 1589
NmuCI GTSAC 3 cut(s) 635, 1129, 1184
NspI RCATGY 2 cut(s) 1560, 1636
OliI CACNNNNGTG 3 cut(s) 674, 1019, 1127
PagI TCATGA 3 cut(s) 498, 540, 717
PciI ACATGT 2 cut(s) 1556, 1632
PctI GAATGC 1 cut(s) 1698
PdmI GAANNNNTTC 3 cut(s) 587, 1310, 1506
PfeI GAWTC 5 cut(s) 766, 1002, 1164, 1233, 1430
PflFI GACNNNGTC 1 cut(s) 1808
PkrI GCNGC 5 cut(s) 76, 646, 973, 1615, 1618
PleI GAGTC 2 cut(s) 1589, 1817
PpsI GAGTC 2 cut(s) 1589, 1817
PscI ACATGT 2 cut(s) 1556, 1632
PshBI ATTAAT 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 725
Psp1406I AACGTT 1 cut(s) 1505
Psp6I CCWGG 2 cut(s) 1114, 1741
PspEI GGTNACC 1 cut(s) 1184
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 2 cut(s) 1114, 1741
PspN4I GGNNCC 1 cut(s) 1120
PspPI GGNCC 2 cut(s) 407, 1024
PstI CTGCAG 2 cut(s) 976, 1618
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 1808
RsaI GTAC 6 cut(s) 167, 505, 1362, 1517, 1637, 1683
RsaNI GTAC 6 cut(s) 166, 504, 1361, 1516, 1636, 1682
RseI CAYNNNNRTG 5 cut(s) 674, 1019, 1127, 1337, 1355
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 4 cut(s) 372, 1062, 1242, 1407
SatI GCNGC 5 cut(s) 75, 645, 972, 1614, 1617
Sau3AI GATC 6 cut(s) 146, 830, 994, 1344, 1379, 1411
Sau96I GGNCC 2 cut(s) 407, 1024
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 2 cut(s) 1589, 1818
ScrFI CCNGG 2 cut(s) 1116, 1743
SduI GDGCHC 4 cut(s) 46, 725, 738, 1297
SfaNI GCATC 3 cut(s) 560, 1126, 1615
SfcI CTRYAG 4 cut(s) 972, 1434, 1614, 1856
SinI GGWCC 2 cut(s) 407, 1024
SmiMI CAYNNNNRTG 5 cut(s) 674, 1019, 1127, 1337, 1355
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 8 cut(s) 67, 131, 300, 507, 584, 1497, 1533, 1547
SsiI CCGC 2 cut(s) 644, 1027
SspI AATATT 1 cut(s) 1714
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 1114, 1741
StyI CCWWGG 2 cut(s) 246, 382
TaaI ACNGT 4 cut(s) 224, 578, 702, 1515
TaiI ACGT 2 cut(s) 1508, 1521
TaqI TCGA 6 cut(s) 273, 1011, 1141, 1491, 1510, 1774
TaqII GACCGA 1 cut(s) 1148
TasI AATT 8 cut(s) 67, 131, 300, 507, 584, 1497, 1533, 1547
TatI WGTACW 3 cut(s) 165, 503, 1635
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 5 cut(s) 766, 1002, 1164, 1233, 1430
Tru1I TTAA 4 cut(s) 372, 1062, 1242, 1407
Tru9I TTAA 4 cut(s) 372, 1062, 1242, 1407
TscAI CASTG 5 cut(s) 981, 1021, 1036, 1582, 1725
TseFI GTSAC 3 cut(s) 635, 1129, 1184
TseI GCWGC 4 cut(s) 74, 971, 1613, 1616
Tsp45I GTSAC 3 cut(s) 635, 1129, 1184
TspDTI ATGAA 9 cut(s) 189, 204, 487, 706, 1156, 1382, 1401, 1463, 1617
TspGWI ACGGA 2 cut(s) 105, 223
TspRI CASTG 5 cut(s) 981, 1021, 1036, 1582, 1725
Tth111I GACNNNGTC 1 cut(s) 1808
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 2 cut(s) 407, 1024
VspI ATTAAT 1 cut(s) 1062
XapI RAATTY 3 cut(s) 67, 131, 584
XceI RCATGY 2 cut(s) 1560, 1636
XmiI GTMKAC 2 cut(s) 273, 1593
XmnI GAANNNNTTC 3 cut(s) 587, 1310, 1506
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.