Rh4CG416500

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008691
Physical Location & Seq
Reverse (-)
66166935 .. 66176495
9561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG416500.1

Sequence Viewer

Length: 1347 bp
ATGAGATTGAATGTTGTTGTATTGCAGATGACAGCATCTCACTTGCCCTACACTCGTAACGGTCTGAAATTCTTCACCAAGAGAGGGGGATTGACCTCGCCGGAAGTGGAGGAAATATGTGACAGAGCTGCCAGAAAGTATGCTAATAGGCTCGCGAAAGTGTCAAGCGTGCTTCGCGTACCACCATCGAGAGTTGATTTCATGAGCTCTTACTCGGAGCACCTCCGAAACATTATCAAGGAGAGAGTCAACCATCCACTACATTATGACACTCCACTGAAAGGATTTCAGATAGTAGTCAATGCTGGTAATGGATCAGGAGGCTTCTTCACATGGGATGTGTTAGACAAGCTAGGAGCAGACACATTTGGGTCTCTACACCTCAAACCAGATGGGATGTTTCCAAACCACATTCCCAACCCAGAGGACAAAGTCGCCATGGCACTTACAAGGGCTGCAGTGCTAGAAAACTCTGCTGATCTTGGAATTGTGTTCGACACTGATGTGGACCGCAGTGGCATTGTAGATAACAAAGGCAATCCCATTAATGGGGACAAGCTTATTGCTTTGATTTCAGCTATTGTATTGAAGGAACACCCTGGAACCACCATTGTGACCGATGCTCGAACAAGTATGGCACTTACAAGATTCATCACCAACAGAGGAGGTCACCATTGTTTATATCGCGTTGGTTACAGGAATGTGATTGACAAGGGAGTCCAGCTTAATAAGGATGGCATTGAAACTCATCTTATGATGGAAACTTCAGGACATGGTGCTCTTAAAGAGAACTTTTTCCTTGATGATGGGGCTTACATGGTGGTGAAGATCATCATTGAGATGGTACGAATGAAGCTTGCTGGATCAGATGAAGGGATTGGAAGTATCATTAACGATCTTGAAGAACCAGAAGAATCTGTAGAGCTAAGGATGAATGTAGTCTCTGAGCCTAGATATGCAAAAGAAAAGGCAATCGAGGCAATTGAAACGTTTCGACAGTACGTTGAGGAGGGAAGAATTGAAGGATGGGAATTAGATACATGTGGAGATTGTTGGGTCAGCGAAGGCTGTCTAGTAGACTCAAATGAGACCTCGGCTGCTGCAGTTGACGCTCACATGTATAGGGCAAAAGTATCAGATGAGGTGCATGGACAACATGGCTGGGTACACATTCGGCAGAGCATTCACAACCCGAATATTGCAGTGAACATGCAATCATCTGTGCCTGGCTGTTGCCAAATGATGACGATAGTTCTTCGAGATAAATTTCTCATAGCTAGTGAAATGGACAGAATCCTTGACATTACTGAGATTGACAAGTATGCCAAAAGTGGAATTGTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004615 GO:0005975 GO:0008150 GO:0008152 GO:0016853 GO:0016866 GO:0016868 GO:0044238 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

448

Amino Acids

49.77

Weight (kDa)

5.76

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_II PF02879 71 - 179 1.2e-21 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 183 - 282 6.6e-11 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 1077
AccII CGCG 3 cut(s) 155, 177, 687
AciI CCGC 1 cut(s) 511
AclI AACGTT 1 cut(s) 989
AclWI GGATC 2 cut(s) 322, 871
AcsI RAATTY 2 cut(s) 68, 1265
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 4 cut(s) 180, 846, 1001, 1167
AfiI CCNNNNNNNGG 4 cut(s) 84, 281, 548, 549
AflIII ACRYGT 2 cut(s) 1040, 1116
AgsI TTSAA 6 cut(s) 10, 589, 743, 902, 986, 1022
AjnI CCWGG 2 cut(s) 598, 1225
AleI CACNNNNGTG 2 cut(s) 503, 611
AluBI AGCT 9 cut(s) 128, 207, 352, 559, 578, 724, 856, 925, 1277
AluI AGCT 9 cut(s) 128, 207, 352, 559, 578, 724, 856, 925, 1277
Alw21I GWGCWC 3 cut(s) 209, 222, 781
Alw26I GTCTC 3 cut(s) 378, 946, 1082
AlwI GGATC 2 cut(s) 322, 871
ApeKI GCWGC 4 cut(s) 128, 455, 1097, 1100
ApoI RAATTY 2 cut(s) 68, 1265
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 3 cut(s) 71, 794, 990
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 67, 646, 662, 835
AvaII GGWCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 209
Bbv12I GWGCWC 3 cut(s) 209, 222, 781
BbvI GCAGC 4 cut(s) 115, 442, 1084, 1087
BccI CCATC 8 cut(s) 193, 261, 386, 728, 751, 800, 835, 1020
BciT130I CCWGG 2 cut(s) 600, 1227
BcoDI GTCTC 3 cut(s) 378, 946, 1082
BfaI CTAG 5 cut(s) 353, 464, 951, 1073, 1278
BfmI CTRYAG 3 cut(s) 456, 918, 1101
BisI GCNGC 4 cut(s) 129, 456, 1098, 1101
BlsI GCNGC 4 cut(s) 130, 457, 1099, 1102
Bme1390I CCNGG 2 cut(s) 600, 1227
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 2 cut(s) 600, 1227
BmsI GCATC 2 cut(s) 44, 610
Bpu10I CCTNAGC 1 cut(s) 926
BsaI GGTCTC 2 cut(s) 378, 1082
BsaJI CCNNGG 3 cut(s) 438, 598, 1092
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 281, 548, 549
BseBI CCWGG 2 cut(s) 600, 1227
BseDI CCNNGG 3 cut(s) 438, 598, 1092
BseGI GGATG 6 cut(s) 253, 343, 402, 739, 936, 1031
BseLI CCNNNNNNNGG 4 cut(s) 84, 281, 548, 549
BseMII CTCAG 2 cut(s) 936, 1299
BseRI GAGGAG 2 cut(s) 678, 1022
BseXI GCAGC 4 cut(s) 115, 442, 1084, 1087
BseYI CCCAGC 1 cut(s) 1161
Bsh1236I CGCG 3 cut(s) 155, 177, 687
BsiHKAI GWGCWC 3 cut(s) 209, 222, 781
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 4 cut(s) 84, 281, 548, 549
BsmAI GTCTC 3 cut(s) 378, 946, 1082
BsmFI GGGAC 1 cut(s) 566
BsmI GAATGC 1 cut(s) 1182
Bso31I GGTCTC 2 cut(s) 378, 1082
Bsp1286I GDGCHC 3 cut(s) 209, 222, 781
Bsp143I GATC 5 cut(s) 314, 478, 828, 863, 895
Bsp19I CCATGG 1 cut(s) 438
Bsp68I TCGCGA 1 cut(s) 155
BspACI CCGC 1 cut(s) 511
BspCNI CTCAG 2 cut(s) 937, 1300
BspFNI CGCG 3 cut(s) 155, 177, 687
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 604
BspMAI CTGCAG 2 cut(s) 460, 1105
BspPI GGATC 2 cut(s) 322, 871
BspTNI GGTCTC 2 cut(s) 378, 1082
BssECI CCNNGG 3 cut(s) 438, 598, 1092
BssMI GATC 5 cut(s) 314, 478, 828, 863, 895
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 2 cut(s) 600, 1227
Bst4CI ACNGT 2 cut(s) 62, 999
BstC8I GCNNGC 3 cut(s) 153, 170, 858
BstDEI CTNAG 3 cut(s) 926, 945, 1308
BstDSI CCRYGG 1 cut(s) 438
BstEII GGTNACC 1 cut(s) 668
BstF5I GGATG 6 cut(s) 253, 343, 402, 739, 936, 1031
BstFNI CGCG 3 cut(s) 155, 177, 687
BstKTI GATC 5 cut(s) 317, 481, 831, 866, 898
BstMAI GTCTC 3 cut(s) 378, 946, 1082
BstMBI GATC 5 cut(s) 314, 478, 828, 863, 895
BstMWI GCNNNNNNNGC 3 cut(s) 174, 977, 1109
BstNI CCWGG 2 cut(s) 600, 1227
BstNSI RCATGY 3 cut(s) 1044, 1120, 1213
BstPI GGTNACC 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 598, 1225
BstSFI CTRYAG 3 cut(s) 456, 918, 1101
BstUI CGCG 3 cut(s) 155, 177, 687
BstV1I GCAGC 4 cut(s) 115, 442, 1084, 1087
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 6 cut(s) 253, 343, 402, 739, 936, 1031
BtsI GCAGTG 3 cut(s) 465, 520, 1209
BtsIMutI CAGTG 5 cut(s) 275, 465, 498, 520, 1209
BtuMI TCGCGA 1 cut(s) 155
Cac8I GCNNGC 3 cut(s) 153, 170, 858
CciI TCATGA 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 179, 845, 1000, 1166
CviQI GTAC 4 cut(s) 179, 845, 1000, 1166
DdeI CTNAG 3 cut(s) 926, 945, 1308
DpnI GATC 5 cut(s) 316, 480, 830, 865, 897
DpnII GATC 5 cut(s) 314, 478, 828, 863, 895
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 2 cut(s) 378, 1082
Eco47I GGWCC 1 cut(s) 508
Eco53kI GAGCTC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 750
Eco91I GGTNACC 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 207
EcoO65I GGTNACC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 598, 1225
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 438
FaqI GGGAC 1 cut(s) 566
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 4 cut(s) 129, 456, 1098, 1101
FokI GGATG 6 cut(s) 240, 350, 409, 746, 943, 1038
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 4 cut(s) 129, 456, 1098, 1101
FspBI CTAG 5 cut(s) 353, 464, 951, 1073, 1278
GluI GCNGC 4 cut(s) 129, 456, 1098, 1101
GsaI CCCAGC 1 cut(s) 1165
HapII CCGG 1 cut(s) 101
HgaI GACGC 1 cut(s) 1118
HincII GTYRAC 2 cut(s) 250, 1108
HindII GTYRAC 2 cut(s) 250, 1108
HindIII AAGCTT 2 cut(s) 557, 854
HinfI GANTC 6 cut(s) 246, 648, 717, 914, 1079, 1293
HpaII CCGG 1 cut(s) 101
HphI GGTGA 4 cut(s) 67, 646, 662, 835
Hpy166II GTNNAC 6 cut(s) 250, 508, 1078, 1108, 1168, 1207
Hpy188I TCNGA 7 cut(s) 66, 217, 227, 291, 868, 946, 1138
Hpy188III TCNNGA 7 cut(s) 154, 189, 202, 318, 768, 899, 1259
Hpy8I GTNNAC 6 cut(s) 250, 508, 1078, 1108, 1168, 1207
HpyAV CCTTC 4 cut(s) 583, 866, 1016, 1058
HpyCH4III ACNGT 2 cut(s) 62, 999
HpyCH4IV ACGT 2 cut(s) 989, 1002
HpyCH4V TGCA 7 cut(s) 25, 458, 959, 1103, 1147, 1202, 1213
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 977, 1109
HpyF3I CTNAG 3 cut(s) 926, 945, 1308
HpySE526I ACGT 2 cut(s) 989, 1002
Kzo9I GATC 5 cut(s) 314, 478, 828, 863, 895
LmnI GCTCC 2 cut(s) 217, 356
Lsp1109I GCAGC 4 cut(s) 115, 442, 1084, 1087
LweI GCATC 2 cut(s) 44, 610
MaeI CTAG 5 cut(s) 353, 464, 951, 1073, 1278
MaeII ACGT 2 cut(s) 989, 1002
MaeIII GTNAC 5 cut(s) 56, 119, 613, 668, 692
MalI GATC 5 cut(s) 316, 480, 830, 865, 897
MboI GATC 5 cut(s) 314, 478, 828, 863, 895
MboII GAAGA 7 cut(s) 64, 319, 838, 914, 923, 1026, 1247
MfeI CAATTG 1 cut(s) 981
MhlI GDGCHC 3 cut(s) 209, 222, 781
MluCI AATT 7 cut(s) 68, 486, 981, 1017, 1031, 1265, 1335
MlyI GAGTC 3 cut(s) 255, 726, 1073
MroXI GAANNNNTTC 3 cut(s) 71, 794, 990
MseI TTAA 4 cut(s) 546, 726, 783, 891
MslI CAYNNNNRTG 4 cut(s) 503, 611, 821, 839
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 600, 1227
MunI CAATTG 1 cut(s) 981
Mva1269I GAATGC 1 cut(s) 1182
MvaI CCWGG 2 cut(s) 600, 1227
MvnI CGCG 3 cut(s) 155, 177, 687
MwoI GCNNNNNNNGC 3 cut(s) 174, 977, 1109
NcoI CCATGG 1 cut(s) 438
NdeII GATC 5 cut(s) 314, 478, 828, 863, 895
NlaIV GGNNCC 1 cut(s) 604
NmeAIII GCCGAG 1 cut(s) 1073
NmuCI GTSAC 3 cut(s) 119, 613, 668
NruI TCGCGA 1 cut(s) 155
NspI RCATGY 3 cut(s) 1044, 1120, 1213
OliI CACNNNNGTG 2 cut(s) 503, 611
PagI TCATGA 1 cut(s) 201
PciI ACATGT 2 cut(s) 1040, 1116
PctI GAATGC 1 cut(s) 1182
PdmI GAANNNNTTC 3 cut(s) 71, 794, 990
PfeI GAWTC 3 cut(s) 648, 914, 1293
PflFI GACNNNGTC 1 cut(s) 431
PkrI GCNGC 4 cut(s) 130, 457, 1099, 1102
PleI GAGTC 3 cut(s) 254, 725, 1073
PpsI GAGTC 3 cut(s) 254, 725, 1073
PscI ACATGT 2 cut(s) 1040, 1116
PshBI ATTAAT 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 209
Psp1406I AACGTT 1 cut(s) 989
Psp6I CCWGG 2 cut(s) 598, 1225
PspEI GGTNACC 1 cut(s) 668
PspFI CCCAGC 1 cut(s) 1161
PspGI CCWGG 2 cut(s) 598, 1225
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 1 cut(s) 508
PstI CTGCAG 2 cut(s) 460, 1105
PsyI GACNNNGTC 1 cut(s) 431
RruI TCGCGA 1 cut(s) 155
RsaI GTAC 4 cut(s) 180, 846, 1001, 1167
RsaNI GTAC 4 cut(s) 179, 845, 1000, 1166
RseI CAYNNNNRTG 4 cut(s) 503, 611, 821, 839
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 4 cut(s) 546, 726, 783, 891
SatI GCNGC 4 cut(s) 129, 456, 1098, 1101
Sau3AI GATC 5 cut(s) 314, 478, 828, 863, 895
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 3 cut(s) 255, 726, 1073
ScrFI CCNGG 2 cut(s) 600, 1227
SduI GDGCHC 3 cut(s) 209, 222, 781
SfaNI GCATC 2 cut(s) 44, 610
SfcI CTRYAG 3 cut(s) 456, 918, 1101
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 4 cut(s) 503, 611, 821, 839
Sse9I AATT 7 cut(s) 68, 486, 981, 1017, 1031, 1265, 1335
SsiI CCGC 1 cut(s) 511
SspI AATATT 1 cut(s) 1198
SspMI CTAG 5 cut(s) 353, 464, 951, 1073, 1278
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 598, 1225
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 62, 999
TaiI ACGT 2 cut(s) 992, 1005
TaqI TCGA 6 cut(s) 188, 495, 625, 975, 994, 1258
TaqII GACCGA 1 cut(s) 632
TasI AATT 7 cut(s) 68, 486, 981, 1017, 1031, 1265, 1335
TfiI GAWTC 3 cut(s) 648, 914, 1293
Tru1I TTAA 4 cut(s) 546, 726, 783, 891
Tru9I TTAA 4 cut(s) 546, 726, 783, 891
TscAI CASTG 5 cut(s) 282, 465, 505, 520, 1209
TseFI GTSAC 3 cut(s) 119, 613, 668
TseI GCWGC 4 cut(s) 128, 455, 1097, 1100
Tsp45I GTSAC 3 cut(s) 119, 613, 668
TspDTI ATGAA 5 cut(s) 190, 640, 866, 885, 947
TspRI CASTG 5 cut(s) 282, 465, 505, 520, 1209
Tth111I GACNNNGTC 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 508
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 2 cut(s) 68, 1265
XceI RCATGY 3 cut(s) 1044, 1120, 1213
XmiI GTMKAC 1 cut(s) 1077
XmnI GAANNNNTTC 3 cut(s) 71, 794, 990
XspI CTAG 5 cut(s) 353, 464, 951, 1073, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.