Rw4G033470

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008691
Physical Location & Seq
Reverse (-)
60656421 .. 60659043
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033470.1

Sequence Viewer

Length: 1839 bp
ATGGCTTCCACTTCAACTTCTTCAATATCTTTGCAAACTAGTGCTCAGAAACCAAGCTTCCCCTCAAATCCAGCAGCCACAACATTCCACACAAACCTTACTTTTCCATATAATCCATTGCCATTTACTAAATTTTTATGTGTAAGATCTTCTAGCACTTCCAAGTACTACAATGAAGTTGTGGTGGATGAAGAGATGGACAGAATTCGGAGGCTCCAAAACGGATCAGATATTCGTGGGGTGGCCTTGGAAGGTGAGAAGGGAAGGTCAGTCGACCTAACGCCTCCGGCTGTGGAGGCCATTGCAGAAAGCTTTGGTGAGTGGGTTATCAATGGCTTGGAGAAGGCGAGAGGACAGCCTGTGGAGAATGTTAAGGTGTCCCTTGGCAGAGACCCTAGAATATCAGGAGTATCTTTGAGTGTAGCTGTATTTTCAGGTCTAGCTCGTGCCGGTTGTCTGGTGTTTGATATGGGACTTGCAACCACTCCTGCTTGCTTCATGAGTACAATTTTGCCTCCGTTTTCCTATGACGCTTCAATCATGATGACAGCATCTCACTTGCCCTACACTCGTAACGGTCTGAAATTCTTCACCAAGAGAGGGGGATTGACCTCGCCGGAAGTGGAGGAAATATGTGACAGAGCTGCCAGAAAGTATGCTAATAGGCTTGCGAAAGTGTCAAGCGTGTTTCGCGTACCACCATCAAGAGTTGATTTCATGAGCTCTTACTCGGAGCACCTCCGAAACATTATCAAGGAGAGAGTCAACCATCCACTATATTATGACACTCCACTGAAAGGATTTCAGGTATGGGATGTTTCCAAACCACATTCCCAACCCAGAGGACAAAGTCGCCATGGCACTTACAAGGGCTGCAGACCAGATGGGATGTTTCCAAACCACATTCCCAACCCAGAGGACAAAGTCGCCATGGCACTTACAAGGGCTGCAGTGCTAGAAAACTCTGCTGATCTTGGAATTGTGTTCGACACTGATGTGGACCGCAGTGGCGTTGTAGATAACAAAGGCAATCCCATTAATGGGGACAAGCTTATTGCTTTGATTTCAGCTATTGTATTGAAGGACCACCCTGGAACCACCATTGTGACTGATGCTCGAACAAGTATGGCACTTACAAGATTCATCACCAACAGAGGAGGTCACCATTGTTTATATCGCGTTGGTTACAGGAATGTGATTGACAAGGGAGTCCAGCTTAATAAGGATGGCATTGAAACTCATCTTATGATGGAAACTTCAGGACATGGTGCTCTTAAAGAGAACTTTTTCCTTGATGATGGGGCTTACATGGTGGTGAAGATCATCATTGAGATGGTACGAATGAAGCTTGCTGGGTCAGATGAAGTTATTGGAAGTATCATTAACGATCTTGAAGAACCAAAAGATTCTGTAGAGCTAAGGATGAATATAGTCTCTGACCCTAGATATGCAAAAGAAAAGGCAATCGAGGCAATTGAAACGTTTCGACAGTACGTGGAGGAGGGAAGAATTGAAGGATGGGAATTAGATACATGTGGAGATTGTTGGGTCAGCGAAGGCTGTCTAGCAGACACAAATGACACCTCGGCTGCTGCAGTTGACGCTCACATGTATAGGGCAAAAGTATCAGATGAGGTGCATGGACAACATGGTTGGGTACACATTCGGCAGAGCATTCACAACCCGAATATTGCAGTGAACATGCAATCATCTGTGCCTGGCTGTTGCCAAATGATGACGATAGTTCTTCGAGATAAATTTCTCATAGCTAGTGAAATGGACAGAATCCTTGACATTAATGAGATTGACAAGTACGCCAAAAGTGGAATTGTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004615 GO:0005975 GO:0008150 GO:0008152 GO:0016853 GO:0016866 GO:0016868 GO:0044238 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

612

Amino Acids

67.61

Weight (kDa)

6.21

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 71 - 217 4.3e-23 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 292 - 343 2.9e-12 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 347 - 447 1.6e-10 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1208
AccI GTMKAC 1 cut(s) 273
AccII CGCG 2 cut(s) 693, 1179
AciI CCGC 1 cut(s) 1003
AclI AACGTT 1 cut(s) 1481
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 4 cut(s) 131, 204, 584, 1757
AcuI CTGAAG 1 cut(s) 1242
AfaI GTAC 7 cut(s) 167, 505, 696, 1338, 1493, 1659, 1814
AfiI CCNNNNNNNGG 4 cut(s) 600, 797, 1040, 1041
AflIII ACRYGT 2 cut(s) 1532, 1608
AgsI TTSAA 8 cut(s) 15, 24, 537, 1081, 1235, 1394, 1478, 1514
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 2 cut(s) 1090, 1717
AleI CACNNNNGTG 2 cut(s) 995, 1103
Alw21I GWGCWC 4 cut(s) 46, 725, 738, 1273
Alw26I GTCTC 2 cut(s) 384, 1438
AlwI GGATC 1 cut(s) 232
AoxI GGCC 2 cut(s) 243, 297
ApeKI GCWGC 6 cut(s) 74, 644, 873, 947, 1589, 1592
ApoI RAATTY 4 cut(s) 131, 204, 584, 1757
AseI ATTAAT 2 cut(s) 1038, 1797
Asp700I GAANNNNTTC 3 cut(s) 587, 1286, 1482
AspS9I GGNCC 2 cut(s) 1000, 1084
AsuHPI GGTGA 6 cut(s) 266, 329, 583, 1138, 1154, 1327
AvaII GGWCC 2 cut(s) 1000, 1084
BanII GRGCYC 1 cut(s) 725
BauI CACGAG 1 cut(s) 444
Bbv12I GWGCWC 4 cut(s) 46, 725, 738, 1273
BbvI GCAGC 6 cut(s) 86, 631, 860, 934, 1576, 1579
BccI CCATC 9 cut(s) 190, 709, 777, 878, 1220, 1243, 1292, 1327, 1512
BciT130I CCWGG 2 cut(s) 1092, 1719
BcoDI GTCTC 2 cut(s) 384, 1438
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 8 cut(s) 39, 153, 396, 440, 956, 1443, 1565, 1770
BfmI CTRYAG 4 cut(s) 874, 948, 1410, 1593
BglII AGATCT 1 cut(s) 146
BisI GCNGC 6 cut(s) 75, 645, 874, 948, 1590, 1593
BlsI GCNGC 6 cut(s) 76, 646, 875, 949, 1591, 1594
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 1092, 1719
Bme18I GGWCC 2 cut(s) 1000, 1084
BmgT120I GGNCC 2 cut(s) 1000, 1084
BmiI GGNNCC 2 cut(s) 215, 1096
BmrFI CCNGG 2 cut(s) 1092, 1719
BmsI GCATC 2 cut(s) 560, 1102
Bpu10I CCTNAGC 1 cut(s) 1418
BsaAI YACGTR 1 cut(s) 1495
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 6 cut(s) 246, 382, 856, 930, 1090, 1584
Bsc4I CCNNNNNNNGG 4 cut(s) 600, 797, 1040, 1041
Bse118I RCCGGY 1 cut(s) 449
Bse3DI GCAATG 2 cut(s) 116, 300
BseBI CCWGG 2 cut(s) 1092, 1719
BseDI CCNNGG 6 cut(s) 246, 382, 856, 930, 1090, 1584
BseGI GGATG 7 cut(s) 193, 769, 820, 894, 1231, 1428, 1523
BseLI CCNNNNNNNGG 4 cut(s) 600, 797, 1040, 1041
BseMI GCAATG 2 cut(s) 116, 300
BseMII CTCAG 1 cut(s) 59
BseRI GAGGAG 2 cut(s) 1170, 1514
BseXI GCAGC 6 cut(s) 86, 631, 860, 934, 1576, 1579
BseYI CCCAGC 1 cut(s) 1352
Bsh1236I CGCG 2 cut(s) 693, 1179
BshFI GGCC 2 cut(s) 245, 299
BsiHKAI GWGCWC 4 cut(s) 46, 725, 738, 1273
BsiSI CCGG 3 cut(s) 287, 450, 617
BslFI GGGAC 3 cut(s) 364, 486, 1058
BslI CCNNNNNNNGG 4 cut(s) 600, 797, 1040, 1041
BsmAI GTCTC 2 cut(s) 384, 1438
BsmFI GGGAC 3 cut(s) 364, 486, 1058
BsmI GAATGC 1 cut(s) 1674
BsnI GGCC 2 cut(s) 245, 299
Bso31I GGTCTC 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 46, 725, 738, 1273
Bsp143I GATC 5 cut(s) 146, 224, 970, 1320, 1387
Bsp19I CCATGG 2 cut(s) 856, 930
BspACI CCGC 1 cut(s) 1003
BspANI GGCC 2 cut(s) 245, 299
BspCNI CTCAG 1 cut(s) 58
BspFNI CGCG 2 cut(s) 693, 1179
BspHI TCATGA 3 cut(s) 498, 540, 717
BspLI GGNNCC 2 cut(s) 215, 1096
BspMAI CTGCAG 3 cut(s) 878, 952, 1597
BspPI GGATC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 384
BsrDI GCAATG 2 cut(s) 116, 300
BsrFI RCCGGY 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 6 cut(s) 246, 382, 856, 930, 1090, 1584
BssMI GATC 5 cut(s) 146, 224, 970, 1320, 1387
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 4 cut(s) 246, 382, 856, 930
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 2 cut(s) 1092, 1719
Bst4CI ACNGT 2 cut(s) 578, 1491
Bst6I CTCTTC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 1495
BstC8I GCNNGC 3 cut(s) 493, 669, 1350
BstDEI CTNAG 2 cut(s) 45, 1418
BstDSI CCRYGG 2 cut(s) 856, 930
BstEII GGTNACC 1 cut(s) 1160
BstF5I GGATG 7 cut(s) 193, 769, 820, 894, 1231, 1428, 1523
BstFNI CGCG 2 cut(s) 693, 1179
BstKTI GATC 5 cut(s) 149, 227, 973, 1323, 1390
BstMAI GTCTC 2 cut(s) 384, 1438
BstMBI GATC 5 cut(s) 146, 224, 970, 1320, 1387
BstMWI GCNNNNNNNGC 4 cut(s) 296, 690, 1469, 1601
BstNI CCWGG 2 cut(s) 1092, 1719
BstNSI RCATGY 3 cut(s) 1536, 1612, 1705
BstPI GGTNACC 1 cut(s) 1160
BstSCI CCNGG 2 cut(s) 1090, 1717
BstSFI CTRYAG 4 cut(s) 874, 948, 1410, 1593
BstUI CGCG 2 cut(s) 693, 1179
BstV1I GCAGC 6 cut(s) 86, 631, 860, 934, 1576, 1579
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 245, 299
BtgI CCRYGG 2 cut(s) 856, 930
BtsCI GGATG 7 cut(s) 193, 769, 820, 894, 1231, 1428, 1523
BtsI GCAGTG 3 cut(s) 957, 1012, 1701
BtsIMutI CAGTG 5 cut(s) 791, 957, 990, 1012, 1701
Cac8I GCNNGC 3 cut(s) 493, 669, 1350
CciI TCATGA 3 cut(s) 498, 540, 717
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 1000, 1084
CseI GACGC 2 cut(s) 539, 1610
Csp6I GTAC 7 cut(s) 166, 504, 695, 1337, 1492, 1658, 1813
CviQI GTAC 7 cut(s) 166, 504, 695, 1337, 1492, 1658, 1813
DdeI CTNAG 2 cut(s) 45, 1418
DpnI GATC 5 cut(s) 148, 226, 972, 1322, 1389
DpnII GATC 5 cut(s) 146, 224, 970, 1320, 1387
DrdI GACNNNNNNGTC 1 cut(s) 1208
DseDI GACNNNNNNGTC 1 cut(s) 1208
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 4 cut(s) 246, 382, 856, 930
Eco24I GRGCYC 1 cut(s) 725
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 2 cut(s) 1000, 1084
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 1242
Eco91I GGTNACC 1 cut(s) 1160
EcoICRI GAGCTC 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 1160
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 1090, 1717
EcoT14I CCWWGG 4 cut(s) 246, 382, 856, 930
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 4 cut(s) 246, 382, 856, 930
FaqI GGGAC 3 cut(s) 364, 486, 1058
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 75, 645, 874, 948, 1590, 1593
FokI GGATG 7 cut(s) 200, 756, 827, 901, 1238, 1435, 1530
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 6 cut(s) 75, 645, 874, 948, 1590, 1593
FspBI CTAG 8 cut(s) 39, 153, 396, 440, 956, 1443, 1565, 1770
GluI GCNGC 6 cut(s) 75, 645, 874, 948, 1590, 1593
GsaI CCCAGC 1 cut(s) 1356
HaeIII GGCC 2 cut(s) 245, 299
HapII CCGG 3 cut(s) 287, 450, 617
HgaI GACGC 2 cut(s) 539, 1610
HincII GTYRAC 3 cut(s) 274, 766, 1600
HindII GTYRAC 3 cut(s) 274, 766, 1600
HindIII AAGCTT 4 cut(s) 55, 310, 1049, 1346
HinfI GANTC 5 cut(s) 762, 1140, 1209, 1406, 1785
HpaII CCGG 3 cut(s) 287, 450, 617
HphI GGTGA 6 cut(s) 266, 329, 583, 1138, 1154, 1327
Hpy166II GTNNAC 6 cut(s) 274, 766, 1000, 1600, 1660, 1699
Hpy188I TCNGA 9 cut(s) 48, 210, 229, 582, 733, 743, 1360, 1438, 1630
Hpy188III TCNNGA 8 cut(s) 405, 499, 541, 705, 718, 1260, 1391, 1751
Hpy8I GTNNAC 6 cut(s) 274, 766, 1000, 1600, 1660, 1699
HpyAV CCTTC 7 cut(s) 245, 253, 258, 337, 1075, 1508, 1550
HpyCH4III ACNGT 2 cut(s) 578, 1491
HpyCH4IV ACGT 2 cut(s) 1481, 1494
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 690, 1469, 1601
HpyF3I CTNAG 2 cut(s) 45, 1418
HpySE526I ACGT 2 cut(s) 1481, 1494
Kzo9I GATC 5 cut(s) 146, 224, 970, 1320, 1387
LmnI GCTCC 2 cut(s) 219, 733
Lsp1109I GCAGC 6 cut(s) 86, 631, 860, 934, 1576, 1579
LweI GCATC 2 cut(s) 560, 1102
MaeI CTAG 8 cut(s) 39, 153, 396, 440, 956, 1443, 1565, 1770
MaeII ACGT 2 cut(s) 1481, 1494
MaeIII GTNAC 5 cut(s) 572, 635, 1105, 1160, 1184
MalI GATC 5 cut(s) 148, 226, 972, 1322, 1389
MboI GATC 5 cut(s) 146, 224, 970, 1320, 1387
MboII GAAGA 8 cut(s) 12, 141, 203, 580, 1330, 1406, 1518, 1739
MfeI CAATTG 1 cut(s) 1473
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 4 cut(s) 46, 725, 738, 1273
MlyI GAGTC 2 cut(s) 771, 1218
MroXI GAANNNNTTC 3 cut(s) 587, 1286, 1482
MseI TTAA 6 cut(s) 372, 1038, 1218, 1275, 1383, 1797
MslI CAYNNNNRTG 4 cut(s) 995, 1103, 1313, 1331
MspI CCGG 3 cut(s) 287, 450, 617
MspR9I CCNGG 2 cut(s) 1092, 1719
MunI CAATTG 1 cut(s) 1473
Mva1269I GAATGC 1 cut(s) 1674
MvaI CCWGG 2 cut(s) 1092, 1719
MvnI CGCG 2 cut(s) 693, 1179
MwoI GCNNNNNNNGC 4 cut(s) 296, 690, 1469, 1601
NcoI CCATGG 2 cut(s) 856, 930
NdeII GATC 5 cut(s) 146, 224, 970, 1320, 1387
NlaIV GGNNCC 2 cut(s) 215, 1096
NmeAIII GCCGAG 1 cut(s) 1565
NmuCI GTSAC 3 cut(s) 635, 1105, 1160
NspI RCATGY 3 cut(s) 1536, 1612, 1705
OliI CACNNNNGTG 2 cut(s) 995, 1103
PagI TCATGA 3 cut(s) 498, 540, 717
PciI ACATGT 2 cut(s) 1532, 1608
PctI GAATGC 1 cut(s) 1674
PdmI GAANNNNTTC 3 cut(s) 587, 1286, 1482
PfeI GAWTC 3 cut(s) 1140, 1406, 1785
PflFI GACNNNGTC 2 cut(s) 849, 923
PkrI GCNGC 6 cut(s) 76, 646, 875, 949, 1591, 1594
PleI GAGTC 2 cut(s) 770, 1217
PpsI GAGTC 2 cut(s) 770, 1217
Ppu21I YACGTR 1 cut(s) 1495
PscI ACATGT 2 cut(s) 1532, 1608
PshBI ATTAAT 2 cut(s) 1038, 1797
Psp124BI GAGCTC 1 cut(s) 725
Psp1406I AACGTT 1 cut(s) 1481
Psp6I CCWGG 2 cut(s) 1090, 1717
PspEI GGTNACC 1 cut(s) 1160
PspFI CCCAGC 1 cut(s) 1352
PspGI CCWGG 2 cut(s) 1090, 1717
PspN4I GGNNCC 2 cut(s) 215, 1096
PspPI GGNCC 2 cut(s) 1000, 1084
PstI CTGCAG 3 cut(s) 878, 952, 1597
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 2 cut(s) 849, 923
RsaI GTAC 7 cut(s) 167, 505, 696, 1338, 1493, 1659, 1814
RsaNI GTAC 7 cut(s) 166, 504, 695, 1337, 1492, 1658, 1813
RseI CAYNNNNRTG 4 cut(s) 995, 1103, 1313, 1331
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 6 cut(s) 372, 1038, 1218, 1275, 1383, 1797
SatI GCNGC 6 cut(s) 75, 645, 874, 948, 1590, 1593
Sau3AI GATC 5 cut(s) 146, 224, 970, 1320, 1387
Sau96I GGNCC 2 cut(s) 1000, 1084
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 2 cut(s) 771, 1218
ScrFI CCNGG 2 cut(s) 1092, 1719
SduI GDGCHC 4 cut(s) 46, 725, 738, 1273
SfaNI GCATC 2 cut(s) 560, 1102
SfcI CTRYAG 4 cut(s) 874, 948, 1410, 1593
SinI GGWCC 2 cut(s) 1000, 1084
SmiMI CAYNNNNRTG 4 cut(s) 995, 1103, 1313, 1331
SpeI ACTAGT 1 cut(s) 38
SsiI CCGC 1 cut(s) 1003
SspI AATATT 1 cut(s) 1690
SspMI CTAG 8 cut(s) 39, 153, 396, 440, 956, 1443, 1565, 1770
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 1090, 1717
StyI CCWWGG 4 cut(s) 246, 382, 856, 930
TaaI ACNGT 2 cut(s) 578, 1491
TaiI ACGT 2 cut(s) 1484, 1497
TaqI TCGA 6 cut(s) 273, 987, 1117, 1467, 1486, 1750
TatI WGTACW 2 cut(s) 165, 503
TfiI GAWTC 3 cut(s) 1140, 1406, 1785
Tru1I TTAA 6 cut(s) 372, 1038, 1218, 1275, 1383, 1797
Tru9I TTAA 6 cut(s) 372, 1038, 1218, 1275, 1383, 1797
TscAI CASTG 5 cut(s) 798, 957, 997, 1012, 1701
TseFI GTSAC 3 cut(s) 635, 1105, 1160
TseI GCWGC 6 cut(s) 74, 644, 873, 947, 1589, 1592
Tsp45I GTSAC 3 cut(s) 635, 1105, 1160
TspDTI ATGAA 8 cut(s) 189, 204, 487, 706, 1132, 1358, 1377, 1439
TspGWI ACGGA 2 cut(s) 237, 507
TspRI CASTG 5 cut(s) 798, 957, 997, 1012, 1701
Tth111I GACNNNGTC 2 cut(s) 849, 923
VpaK11BI GGWCC 2 cut(s) 1000, 1084
VspI ATTAAT 2 cut(s) 1038, 1797
XapI RAATTY 4 cut(s) 131, 204, 584, 1757
XceI RCATGY 3 cut(s) 1536, 1612, 1705
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 3 cut(s) 587, 1286, 1482
XspI CTAG 8 cut(s) 39, 153, 396, 440, 956, 1443, 1565, 1770
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.