RchiOBHm_Chr4g0441801

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008691
Physical Location & Seq
Reverse (-)
63628440 .. 63630926
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40959

Sequence Viewer

Length: 1863 bp
ATGGCTTCCACTTCAACTTCTTCAATATCTTTGCAAACTAGTGCTCAGAAACCAAGCTTCCCCTCAAATTCAGCAACCACAACATTCCACACAAACCTTACTTTTCCATATAATCCATTGCCATTTACTAAATTTTTATGTGTAAGATCTTCTAGCACTTCCAAGTACTACAATGAAGTTGTGGTGGATGAAGAGATGGACAGAATTCGGAGGCTCCAAAACGGATCAGATATTCGTGGGGTGGCCTTGGAAGGTGAGAAGGGAAGGTCAGTCGACCTAACGCCTCCGGCTGTGGAGGCCATTGCAGAAAGCTTTGGTGAGTGGGTTATCAATGGCTTGGAGAAGGAGAGAGGACAGCCTGTGGAGAATGTTAAGGTGTCCCTTGGCAGAGACCCTAGAATATCAGGAGCATCTTTGAGTGTAGCTGTATTTTCAGGTCTAGCTCGTGCCGGTTGTCTGGTGTTTGATATGGGACTTGCAACCACTCCTGCTTGCTTCATGAGTACAATTTTGCCTCCGTTTTCCTATGACGCTTCAATCATGATGACAGCATCTCACTTGCCCTACACTCGTAACGGTCTGAAATTCTTCACCAAGAGAGGGGGATTGACCTCGCCGGAAGTGGAGGAAATATGTGACAGAGCTGCCAGAAAGTATGCTAATAGGCTTGCGAAAGTGTCAAGCGTGCTTCGCGTACCACCATCAAGAGTTGATTTCATGAGCTCTTACTCGGAGCACCTCCGAAACATTATCAAGGAGAGAGTCAACCATCCACTATATTATGACACTCCACTGAAAGGATTTCAGATAGTAGTCAATGCTGGTAATGGATCAGGAGGCTTCTTCACATGGGATGTGTTAGACAAGCTAGGAGCAGACACATTTGGGTCTCTACACCTCAAACCAGATGGGATGTTTCCAAACCACATTCCCAACCCAGAGGACAAAGTCGCCATGGCACTTACAAGGGCTGCAGTGCTAGAAAACTCTGCTGATCTTGGAATTGTGTTCGACACTGATGTGGACCGCAGTGGCGTTGTAGATAACAAAGGCAATCCCATTAATGGGGACAAGCTTATTGCTTTGATTTCAGCTATTGTATTGAAGGAACACCCTGGAACCACCATTGTGACTGATGCTCGAACAAGTATGGCACTTACAAGATTCATCACCAACAGAGGAGGTCACCATTGTTTATATCGCGTTGGTTACAGGAATGTGATTGACAAGGGAGTCCAGCTTAATAAGGATGGCATTGAAACTCATCTTATGATGGAAACTTCAGGACATGGTGCTCTTAAAGAGAACTTTTTCCTTGATGATGGGGCTTACATGGTGGTGAAGATCATCATTGAGATGGTACGAATGAAGCTTGCTGGGTCAGATGAAGGTATTGGAAGTATCATTAACGATCTTGAAGAACCAAAAGATTCTGTAGAGCTAAGGATGAATGTAGTCTCTGACCCTAGATATGCAAAAGAAAAGGCAATCGAGGCAATTGAAACGTTTCGACAGTACGTGGAGGAGGGAAGAATTGAAGGATGGGAATTAGATACATGTGGAGATTGTTGGGTCAGCGAAGGCTGTCTAGCAGACATAAATGAAACCTCGGCTGCTGCAGTTGACGCTCACATGTATAGGGCAAAAGTATCAGATGAGGTGCATGGACAACATGGTTGGGTACACATTCGGCAGAGCATTCACAACCCGAATATTGCAGTGAACATGCAATCATCTGTGCTTGGCTGTTGCCAAATGATGACGATAGTTCTTCGAGATAAATTTCTCATAGCTAGTGAAATGGACAGAATCCTTGACATTACTGAGATTGACAAGTACGCCAAAAGTGGAATTGTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004615 GO:0005975 GO:0008150 GO:0008152 GO:0016853 GO:0016866 GO:0016868 GO:0044238 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

620

Amino Acids

68.18

Weight (kDa)

5.73

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 71 - 217 1.8e-23 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 243 - 351 1.9e-21 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 355 - 454 1.1e-10 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1232
AccI GTMKAC 1 cut(s) 273
AccII CGCG 2 cut(s) 693, 1203
AciI CCGC 1 cut(s) 1027
AclI AACGTT 1 cut(s) 1505
AclWI GGATC 2 cut(s) 232, 838
AcsI RAATTY 5 cut(s) 67, 131, 204, 584, 1781
AcuI CTGAAG 1 cut(s) 1266
AfaI GTAC 7 cut(s) 167, 505, 696, 1362, 1517, 1683, 1838
AfiI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
AflIII ACRYGT 2 cut(s) 1556, 1632
AgsI TTSAA 8 cut(s) 15, 24, 537, 1105, 1259, 1418, 1502, 1538
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 1 cut(s) 1114
AleI CACNNNNGTG 2 cut(s) 1019, 1127
Alw21I GWGCWC 4 cut(s) 46, 725, 738, 1297
Alw26I GTCTC 3 cut(s) 384, 894, 1462
AlwI GGATC 2 cut(s) 232, 838
AoxI GGCC 2 cut(s) 243, 297
ApeKI GCWGC 4 cut(s) 644, 971, 1613, 1616
ApoI RAATTY 5 cut(s) 67, 131, 204, 584, 1781
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 3 cut(s) 587, 1310, 1506
AspS9I GGNCC 1 cut(s) 1024
AsuHPI GGTGA 6 cut(s) 266, 329, 583, 1162, 1178, 1351
AvaII GGWCC 1 cut(s) 1024
BanII GRGCYC 1 cut(s) 725
BauI CACGAG 1 cut(s) 444
Bbv12I GWGCWC 4 cut(s) 46, 725, 738, 1297
BbvI GCAGC 4 cut(s) 631, 958, 1600, 1603
BccI CCATC 9 cut(s) 190, 709, 777, 902, 1244, 1267, 1316, 1351, 1536
BciT130I CCWGG 1 cut(s) 1116
BcoDI GTCTC 3 cut(s) 384, 894, 1462
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 9 cut(s) 39, 153, 396, 440, 869, 980, 1467, 1589, 1794
BfmI CTRYAG 3 cut(s) 972, 1434, 1617
BglII AGATCT 1 cut(s) 146
BisI GCNGC 4 cut(s) 645, 972, 1614, 1617
BlsI GCNGC 4 cut(s) 646, 973, 1615, 1618
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 1116
Bme18I GGWCC 1 cut(s) 1024
BmgT120I GGNCC 1 cut(s) 1024
BmiI GGNNCC 2 cut(s) 215, 1120
BmrFI CCNGG 1 cut(s) 1116
BmsI GCATC 3 cut(s) 419, 560, 1126
Bpu10I CCTNAGC 1 cut(s) 1442
BsaAI YACGTR 1 cut(s) 1519
BsaI GGTCTC 2 cut(s) 384, 894
BsaJI CCNNGG 5 cut(s) 246, 382, 954, 1114, 1608
Bsc4I CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
Bse118I RCCGGY 1 cut(s) 449
Bse3DI GCAATG 2 cut(s) 116, 300
BseBI CCWGG 1 cut(s) 1116
BseDI CCNNGG 5 cut(s) 246, 382, 954, 1114, 1608
BseGI GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BseLI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
BseMI GCAATG 2 cut(s) 116, 300
BseMII CTCAG 2 cut(s) 59, 1815
BseRI GAGGAG 2 cut(s) 1194, 1538
BseXI GCAGC 4 cut(s) 631, 958, 1600, 1603
BseYI CCCAGC 1 cut(s) 1376
Bsh1236I CGCG 2 cut(s) 693, 1203
BshFI GGCC 2 cut(s) 245, 299
BsiHKAI GWGCWC 4 cut(s) 46, 725, 738, 1297
BsiSI CCGG 3 cut(s) 287, 450, 617
BslFI GGGAC 3 cut(s) 364, 486, 1082
BslI CCNNNNNNNGG 4 cut(s) 600, 797, 1064, 1065
BsmAI GTCTC 3 cut(s) 384, 894, 1462
BsmFI GGGAC 3 cut(s) 364, 486, 1082
BsmI GAATGC 1 cut(s) 1698
BsnI GGCC 2 cut(s) 245, 299
Bso31I GGTCTC 2 cut(s) 384, 894
Bsp1286I GDGCHC 4 cut(s) 46, 725, 738, 1297
Bsp143I GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
Bsp19I CCATGG 1 cut(s) 954
BspACI CCGC 1 cut(s) 1027
BspANI GGCC 2 cut(s) 245, 299
BspCNI CTCAG 2 cut(s) 58, 1816
BspFNI CGCG 2 cut(s) 693, 1203
BspHI TCATGA 3 cut(s) 498, 540, 717
BspLI GGNNCC 2 cut(s) 215, 1120
BspMAI CTGCAG 2 cut(s) 976, 1621
BspPI GGATC 2 cut(s) 232, 838
BspTNI GGTCTC 2 cut(s) 384, 894
BsrDI GCAATG 2 cut(s) 116, 300
BsrFI RCCGGY 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 5 cut(s) 246, 382, 954, 1114, 1608
BssMI GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 3 cut(s) 246, 382, 954
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 1116
Bst4CI ACNGT 2 cut(s) 578, 1515
Bst6I CTCTTC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 1519
BstC8I GCNNGC 4 cut(s) 493, 669, 686, 1374
BstDEI CTNAG 3 cut(s) 45, 1442, 1824
BstDSI CCRYGG 1 cut(s) 954
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BstFNI CGCG 2 cut(s) 693, 1203
BstKTI GATC 6 cut(s) 149, 227, 833, 997, 1347, 1414
BstMAI GTCTC 3 cut(s) 384, 894, 1462
BstMBI GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
BstMWI GCNNNNNNNGC 4 cut(s) 296, 690, 1493, 1625
BstNI CCWGG 1 cut(s) 1116
BstNSI RCATGY 3 cut(s) 1560, 1636, 1729
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 1 cut(s) 1114
BstSFI CTRYAG 3 cut(s) 972, 1434, 1617
BstUI CGCG 2 cut(s) 693, 1203
BstV1I GCAGC 4 cut(s) 631, 958, 1600, 1603
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 245, 299
BtgI CCRYGG 1 cut(s) 954
BtsCI GGATG 7 cut(s) 193, 769, 859, 918, 1255, 1452, 1547
BtsI GCAGTG 3 cut(s) 981, 1036, 1725
BtsIMutI CAGTG 5 cut(s) 791, 981, 1014, 1036, 1725
Cac8I GCNNGC 4 cut(s) 493, 669, 686, 1374
CciI TCATGA 3 cut(s) 498, 540, 717
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 1024
CseI GACGC 2 cut(s) 539, 1634
Csp6I GTAC 7 cut(s) 166, 504, 695, 1361, 1516, 1682, 1837
CviQI GTAC 7 cut(s) 166, 504, 695, 1361, 1516, 1682, 1837
DdeI CTNAG 3 cut(s) 45, 1442, 1824
DpnI GATC 6 cut(s) 148, 226, 832, 996, 1346, 1413
DpnII GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
DrdI GACNNNNNNGTC 1 cut(s) 1232
DseDI GACNNNNNNGTC 1 cut(s) 1232
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 3 cut(s) 246, 382, 954
Eco24I GRGCYC 1 cut(s) 725
Eco31I GGTCTC 2 cut(s) 384, 894
Eco47I GGWCC 1 cut(s) 1024
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 1266
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 1184
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 1114
EcoT14I CCWWGG 3 cut(s) 246, 382, 954
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 3 cut(s) 246, 382, 954
FaqI GGGAC 3 cut(s) 364, 486, 1082
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 645, 972, 1614, 1617
FokI GGATG 7 cut(s) 200, 756, 866, 925, 1262, 1459, 1554
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 4 cut(s) 645, 972, 1614, 1617
FspBI CTAG 9 cut(s) 39, 153, 396, 440, 869, 980, 1467, 1589, 1794
GluI GCNGC 4 cut(s) 645, 972, 1614, 1617
GsaI CCCAGC 1 cut(s) 1380
HaeIII GGCC 2 cut(s) 245, 299
HapII CCGG 3 cut(s) 287, 450, 617
HgaI GACGC 2 cut(s) 539, 1634
HincII GTYRAC 3 cut(s) 274, 766, 1624
HindII GTYRAC 3 cut(s) 274, 766, 1624
HindIII AAGCTT 4 cut(s) 55, 310, 1073, 1370
HinfI GANTC 5 cut(s) 762, 1164, 1233, 1430, 1809
HpaII CCGG 3 cut(s) 287, 450, 617
HphI GGTGA 6 cut(s) 266, 329, 583, 1162, 1178, 1351
Hpy166II GTNNAC 6 cut(s) 274, 766, 1024, 1624, 1684, 1723
Hpy188III TCNNGA 9 cut(s) 405, 499, 541, 705, 718, 834, 1284, 1415, 1775
Hpy8I GTNNAC 6 cut(s) 274, 766, 1024, 1624, 1684, 1723
HpyAV CCTTC 8 cut(s) 245, 253, 258, 337, 1099, 1382, 1532, 1574
HpyCH4III ACNGT 2 cut(s) 578, 1515
HpyCH4IV ACGT 2 cut(s) 1505, 1518
HpyCH4V TGCA 9 cut(s) 34, 305, 479, 974, 1475, 1619, 1663, 1718, 1729
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 690, 1493, 1625
HpyF3I CTNAG 3 cut(s) 45, 1442, 1824
HpySE526I ACGT 2 cut(s) 1505, 1518
Kzo9I GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
LmnI GCTCC 4 cut(s) 219, 407, 733, 872
Lsp1109I GCAGC 4 cut(s) 631, 958, 1600, 1603
LweI GCATC 3 cut(s) 419, 560, 1126
MaeI CTAG 9 cut(s) 39, 153, 396, 440, 869, 980, 1467, 1589, 1794
MaeII ACGT 2 cut(s) 1505, 1518
MaeIII GTNAC 5 cut(s) 572, 635, 1129, 1184, 1208
MalI GATC 6 cut(s) 148, 226, 832, 996, 1346, 1413
MboI GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
MboII GAAGA 9 cut(s) 12, 141, 203, 580, 835, 1354, 1430, 1542, 1763
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 4 cut(s) 46, 725, 738, 1297
MlyI GAGTC 2 cut(s) 771, 1242
MroXI GAANNNNTTC 3 cut(s) 587, 1310, 1506
MseI TTAA 5 cut(s) 372, 1062, 1242, 1299, 1407
MslI CAYNNNNRTG 4 cut(s) 1019, 1127, 1337, 1355
MspI CCGG 3 cut(s) 287, 450, 617
MspR9I CCNGG 1 cut(s) 1116
MunI CAATTG 1 cut(s) 1497
Mva1269I GAATGC 1 cut(s) 1698
MvaI CCWGG 1 cut(s) 1116
MvnI CGCG 2 cut(s) 693, 1203
MwoI GCNNNNNNNGC 4 cut(s) 296, 690, 1493, 1625
NcoI CCATGG 1 cut(s) 954
NdeII GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
NlaIV GGNNCC 2 cut(s) 215, 1120
NmeAIII GCCGAG 1 cut(s) 1589
NmuCI GTSAC 3 cut(s) 635, 1129, 1184
NspI RCATGY 3 cut(s) 1560, 1636, 1729
OliI CACNNNNGTG 2 cut(s) 1019, 1127
PagI TCATGA 3 cut(s) 498, 540, 717
PciI ACATGT 2 cut(s) 1556, 1632
PctI GAATGC 1 cut(s) 1698
PdmI GAANNNNTTC 3 cut(s) 587, 1310, 1506
PfeI GAWTC 3 cut(s) 1164, 1430, 1809
PflFI GACNNNGTC 1 cut(s) 947
PkrI GCNGC 4 cut(s) 646, 973, 1615, 1618
PleI GAGTC 2 cut(s) 770, 1241
PpsI GAGTC 2 cut(s) 770, 1241
Ppu21I YACGTR 1 cut(s) 1519
PscI ACATGT 2 cut(s) 1556, 1632
PshBI ATTAAT 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 725
Psp1406I AACGTT 1 cut(s) 1505
Psp6I CCWGG 1 cut(s) 1114
PspEI GGTNACC 1 cut(s) 1184
PspFI CCCAGC 1 cut(s) 1376
PspGI CCWGG 1 cut(s) 1114
PspN4I GGNNCC 2 cut(s) 215, 1120
PspPI GGNCC 1 cut(s) 1024
PstI CTGCAG 2 cut(s) 976, 1621
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 947
RsaI GTAC 7 cut(s) 167, 505, 696, 1362, 1517, 1683, 1838
RsaNI GTAC 7 cut(s) 166, 504, 695, 1361, 1516, 1682, 1837
RseI CAYNNNNRTG 4 cut(s) 1019, 1127, 1337, 1355
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 5 cut(s) 372, 1062, 1242, 1299, 1407
SatI GCNGC 4 cut(s) 645, 972, 1614, 1617
Sau3AI GATC 6 cut(s) 146, 224, 830, 994, 1344, 1411
Sau96I GGNCC 1 cut(s) 1024
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 2 cut(s) 771, 1242
ScrFI CCNGG 1 cut(s) 1116
SduI GDGCHC 4 cut(s) 46, 725, 738, 1297
SfaNI GCATC 3 cut(s) 419, 560, 1126
SfcI CTRYAG 3 cut(s) 972, 1434, 1617
SinI GGWCC 1 cut(s) 1024
SmiMI CAYNNNNRTG 4 cut(s) 1019, 1127, 1337, 1355
SpeI ACTAGT 1 cut(s) 38
SsiI CCGC 1 cut(s) 1027
SspI AATATT 1 cut(s) 1714
SspMI CTAG 9 cut(s) 39, 153, 396, 440, 869, 980, 1467, 1589, 1794
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 1 cut(s) 1114
StyI CCWWGG 3 cut(s) 246, 382, 954
TaaI ACNGT 2 cut(s) 578, 1515
TaiI ACGT 2 cut(s) 1508, 1521
TaqI TCGA 6 cut(s) 273, 1011, 1141, 1491, 1510, 1774
TatI WGTACW 2 cut(s) 165, 503
TfiI GAWTC 3 cut(s) 1164, 1430, 1809
Tru1I TTAA 5 cut(s) 372, 1062, 1242, 1299, 1407
Tru9I TTAA 5 cut(s) 372, 1062, 1242, 1299, 1407
TscAI CASTG 5 cut(s) 798, 981, 1021, 1036, 1725
TseFI GTSAC 3 cut(s) 635, 1129, 1184
TseI GCWGC 4 cut(s) 644, 971, 1613, 1616
Tsp45I GTSAC 3 cut(s) 635, 1129, 1184
TspDTI ATGAA 9 cut(s) 189, 204, 487, 706, 1156, 1382, 1401, 1463, 1617
TspGWI ACGGA 2 cut(s) 237, 507
TspRI CASTG 5 cut(s) 798, 981, 1021, 1036, 1725
Tth111I GACNNNGTC 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 1024
VspI ATTAAT 1 cut(s) 1062
XapI RAATTY 5 cut(s) 67, 131, 204, 584, 1781
XceI RCATGY 3 cut(s) 1560, 1636, 1729
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 3 cut(s) 587, 1310, 1506
XspI CTAG 9 cut(s) 39, 153, 396, 440, 869, 980, 1467, 1589, 1794
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.