FvH4_4g33390

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014090
Physical Location & Seq
Forward (+)
31630608 .. 31635007
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33390.t1

Sequence Viewer

Length: 1902 bp
ATGACAAGCTTCGGAGACATTCTCAAATTGGAGAGAGAGAAAGAGATGATGCTCACGACGACAAGTACTAGTCTCGATCACAGCATCGTCGACAGAGTTCTAGCCAGTTTAGGCAAAGGCTTCGATATAACATCGGATTTCCGGCTTCAGTATTGCAAAGGCAAACGAAGGTTGGTTGCCCTAAACGACGGCGAGAAGAGGGAGCTCGTGGTACCTGGTTTTGGTACCATTCCGGACGTCTCCGGTGACATTAAATGCGACAAGGGAGACCGGACGAGGTACCAGACGGACATGTTGAGGTTCAGTGAGATGTCGGAGCATATCAACCAGAGGTCGAGTGTCACCGGGAAAGTTCCGTCGGGGTTGTTCAATTCGTTGTTTGGGTTTGATAGTGGTTCGTGGGGGAGTGATGCGGCCAATATCAAATGCTTGGGTGTGGATGGCTATTTCATTATCTTCTTCAGTGTTCATATTGATAGGTACCCTCTTGTTCTCGCCGATGAAGTTCGCGATGCTGTTCCGGCCACTTGGGATCCTCTTGCCATTTCAAGATTCATAGAGAAATTTGGGACGCACATCGTGGTGGGTTTGAGTGTTGGGGGTCAGGATGTGGTGTCAGTTAAACAAGAGAAATCATCAACTTTAGGGCCATCAGACGTTAAGAAACACTTAAACGAGCTTGGAGATCAGCACTTTACAGGGACTTGTAATTTCTCCCCACTCCTCTCCAAAGCAAAAGACCATAGACACAAGGCACCACAGGCATTCAATATCTTTGATTCTCCGCAATCCGTCTCCATTAACGATTTCACCACCGCAAACAAAAAAGATGGAATCACTGTGATAAGCTCTAAAAAGGGAGGTGATCCATATGCCAGTAGCCACAGTGAATGGCTTCAAACAGTTCCGTCGATGCCGGACGCTATTCAGTACAACTTCATTCCTATCACTTCTCTCCTTAAAGGTGTTCCCGGCAAAGGTTTCTTGTCCCATGCCATCAACCTCTATCTCAGATACAAGCCTCCTATAGCTGATTTGCCCTACTTTCTTGAATATCAAGTATCACACAAAGTTTGGGCTCCCATTCATAATGACCTACGTTTAGGTCCTGCCACAAATAGCAAATCTAATCCATCATCACCTGCTATTCACTTCAGCTTTATGGGTCCTAAATTATATGTAAACACCACACAGGTTAGAGTTGGAAATAACGCCCCCATCACAGGAATGCGATTGTTTCTTGAGGGCATGAAATGCAACAGGCTAGCCATCCACCTCCAACATCTTGCAAACACTCCATTGATGTTGGATAACAAGATCGATGACGCAAAGATATGGCGAGGAACAGATGAGATCCACGATGACCGGTACTTTGAAGCAACAAACGGGAACAAGTTCTCCCACGTTTGCACGGCGCCGGTAACATACGACCCGAAGTGGATGTCTTCAGGCTTAACCTCGTCCTTCGTTGTCTCTGGAGCACAGCTTGTGGTGAAGAAACACGGCTCAAAGAATGTTCTCCATCTTCGGCTCTTGTTCTCCAAAGTATTGGACTCTTTCTTAGTACAATCAAATTGGGAAGAGTCATGCTCCTCAACTAAATCGTCATCCTCCGGCCTTTTGAATGCGATGAGTAGACCAATTTCCGGGAACTATTGGGGGAAAGAGAAAGTAGAACCTGCGGCGGAGCAAGTGATATTGGATTCGAGTGTTTTTCCGGCAGGACCTCCGGTGCCATTGCCAAGGCAAAAGTTTGTGAAGCATGTAAACACCTCACATGTATGCAAAGGGCCGCAGGATAGTCCTGGCCATTGGTTAGTCACCGGAGCAAAGCTCAACTTGGTCAAGGGAAAGATTGGGTTGCAAGTCAAGTTCTCTCTGTTAAACCTAGAAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

634

Amino Acids

69.9

Weight (kDa)

8.96

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 123 - 336 4.2e-28 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1150
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 2 cut(s) 1150, 1687
Acc65I GGTACC 4 cut(s) 211, 224, 279, 480
AccB1I GGYRCC 7 cut(s) 211, 224, 279, 480, 754, 1414, 1732
AccI GTMKAC 2 cut(s) 90, 1636
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 6 cut(s) 413, 785, 816, 1682, 1685, 1793
AclWI GGATC 4 cut(s) 527, 540, 860, 1348
AcoI YGGCCR 3 cut(s) 414, 522, 1807
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 4 cut(s) 131, 445, 1138, 1431
AcyI GRCGYC 2 cut(s) 237, 1415
AdeI CACNNNGTG 1 cut(s) 580
AfaI GTAC 8 cut(s) 67, 213, 226, 281, 482, 932, 1370, 1566
AfiI CCNNNNNNNGG 4 cut(s) 221, 977, 1223, 1646
AflIII ACRYGT 2 cut(s) 291, 1777
AgeI ACCGGT 1 cut(s) 1365
AgsI TTSAA 7 cut(s) 370, 549, 769, 899, 1052, 1376, 1624
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 2 cut(s) 214, 1804
AloI GAACNNNNNNTCC 2 cut(s) 1382, 1414
AluBI AGCT 8 cut(s) 9, 205, 679, 849, 1031, 1158, 1486, 1834
AluI AGCT 8 cut(s) 9, 205, 679, 849, 1031, 1158, 1486, 1834
Alw21I GWGCWC 2 cut(s) 207, 1483
Alw26I GTCTC 6 cut(s) 9, 77, 244, 261, 799, 1477
AlwI GGATC 4 cut(s) 527, 540, 860, 1348
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 6 cut(s) 414, 522, 647, 1615, 1790, 1807
ApoI RAATTY 1 cut(s) 563
ArsI GACNNNNNNTTYG 4 cut(s) 1355, 1387, 1427, 1459
AsiGI ACCGGT 1 cut(s) 1365
Asp700I GAANNNNTTC 2 cut(s) 894, 1394
Asp718I GGTACC 4 cut(s) 211, 224, 279, 480
AspLEI GCGC 1 cut(s) 1417
AspS9I GGNCC 5 cut(s) 647, 1106, 1166, 1724, 1790
AsuC2I CCSGG 3 cut(s) 346, 972, 1648
AsuHPI GGTGA 7 cut(s) 257, 334, 802, 875, 1131, 1504, 1813
AsuNHI GCTAGC 1 cut(s) 1264
AvaII GGWCC 3 cut(s) 1106, 1166, 1724
BalI TGGCCA 1 cut(s) 1809
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 7 cut(s) 211, 224, 279, 480, 754, 1414, 1732
BanII GRGCYC 2 cut(s) 207, 1081
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 1437
Bbv12I GWGCWC 2 cut(s) 207, 1483
BccI CCATC 8 cut(s) 434, 658, 824, 1004, 1141, 1226, 1277, 1530
BceAI ACGGC 3 cut(s) 205, 1428, 1519
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 2 cut(s) 216, 1806
BcnI CCSGG 3 cut(s) 346, 972, 1648
BcoDI GTCTC 6 cut(s) 9, 77, 244, 261, 799, 1477
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 4 cut(s) 69, 101, 1265, 1889
BfmI CTRYAG 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 1418
BfuAI ACCTGC 2 cut(s) 1150, 1687
BisI GCNGC 3 cut(s) 414, 1683, 1793
BlsI GCNGC 3 cut(s) 415, 1684, 1794
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 5 cut(s) 216, 346, 972, 1648, 1806
Bme18I GGWCC 3 cut(s) 1106, 1166, 1724
BmgT120I GGNCC 5 cut(s) 647, 1106, 1166, 1724, 1790
BmrFI CCNGG 5 cut(s) 216, 346, 972, 1648, 1806
BmsI GCATC 5 cut(s) 39, 93, 400, 502, 903
BmtI GCTAGC 1 cut(s) 1268
BpiI GAAGAC 1 cut(s) 1437
BplI GAGNNNNNCTC 5 cut(s) 38, 1574, 1606, 1818, 1850
BpmI CTGGAG 1 cut(s) 1497
BpuEI CTTGAG 1 cut(s) 1262
BpuMI CCSGG 3 cut(s) 346, 972, 1648
Bsa29I ATCGAT 1 cut(s) 1320
BsaHI GRCGYC 2 cut(s) 237, 1415
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 1742
BsaWI WCCGGW 6 cut(s) 232, 242, 270, 1365, 1729, 1823
BsaXI ACNNNNNCTCC 2 cut(s) 1382, 1412
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 977, 1223, 1646
Bse118I RCCGGY 2 cut(s) 1365, 1417
Bse1I ACTGG 2 cut(s) 105, 876
Bse3DI GCAATG 1 cut(s) 1736
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 2 cut(s) 216, 1806
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 1 cut(s) 1742
BseGI GGATG 5 cut(s) 445, 613, 1269, 1446, 1607
BseLI CCNNNNNNNGG 4 cut(s) 221, 977, 1223, 1646
BseMI GCAATG 1 cut(s) 1736
BseMII CTCAG 1 cut(s) 1024
BseNI ACTGG 2 cut(s) 105, 876
BseRI GAGGAG 2 cut(s) 713, 1582
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 6 cut(s) 416, 524, 649, 1617, 1792, 1809
BshNI GGYRCC 7 cut(s) 211, 224, 279, 480, 754, 1414, 1732
BshTI ACCGGT 1 cut(s) 1365
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 2 cut(s) 207, 1483
BslFI GGGAC 3 cut(s) 583, 715, 973
BslI CCNNNNNNNGG 4 cut(s) 221, 977, 1223, 1646
BsmAI GTCTC 6 cut(s) 9, 77, 244, 261, 799, 1477
BsmBI CGTCTC 2 cut(s) 244, 799
BsmFI GGGAC 3 cut(s) 583, 715, 973
BsmI GAATGC 3 cut(s) 764, 1233, 1630
BsnI GGCC 6 cut(s) 416, 524, 649, 1617, 1792, 1809
Bso31I GGTCTC 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 207, 1081, 1483
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 6 cut(s) 413, 785, 816, 1682, 1685, 1793
BspANI GGCC 6 cut(s) 416, 524, 649, 1617, 1792, 1809
BspCNI CTCAG 1 cut(s) 1023
BspDI ATCGAT 1 cut(s) 1320
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 510
BspMI ACCTGC 2 cut(s) 1150, 1687
BspOI GCTAGC 1 cut(s) 1268
BspPI GGATC 4 cut(s) 527, 540, 860, 1348
BspT107I GGYRCC 7 cut(s) 211, 224, 279, 480, 754, 1414, 1732
BspTNI GGTCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 1736
BsrFI RCCGGY 2 cut(s) 1365, 1417
BsrI ACTGG 2 cut(s) 105, 876
BssAI RCCGGY 2 cut(s) 1365, 1417
BssECI CCNNGG 1 cut(s) 1742
BssMI GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
BssNI GRCGYC 2 cut(s) 237, 1415
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 1742
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 216, 1806
Bst4CI ACNGT 3 cut(s) 841, 887, 904
Bst6I CTCTTC 2 cut(s) 191, 1575
BstACI GRCGYC 2 cut(s) 237, 1415
BstAPI GCANNNNNTGC 1 cut(s) 1254
BstC8I GCNNGC 1 cut(s) 1266
BstDEI CTNAG 2 cut(s) 1010, 1561
BstF5I GGATG 5 cut(s) 445, 613, 1269, 1446, 1607
BstFNI CGCG 1 cut(s) 510
BstH2I RGCGCY 1 cut(s) 1418
BstHHI GCGC 1 cut(s) 1417
BstKTI GATC 6 cut(s) 79, 535, 688, 868, 1320, 1356
BstMAI GTCTC 6 cut(s) 9, 77, 244, 261, 799, 1477
BstMBI GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
BstMWI GCNNNNNNNGC 3 cut(s) 521, 761, 1254
BstNI CCWGG 2 cut(s) 216, 1806
BstNSI RCATGY 3 cut(s) 295, 1766, 1781
BstSCI CCNGG 5 cut(s) 214, 344, 970, 1646, 1804
BstSFI CTRYAG 1 cut(s) 1026
BstUI CGCG 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 1437
BstX2I RGATCY 2 cut(s) 532, 1353
BstXI CCANNNNNNTGG 1 cut(s) 1549
BstYI RGATCY 2 cut(s) 532, 1353
Bsu15I ATCGAT 1 cut(s) 1320
BsuRI GGCC 6 cut(s) 416, 524, 649, 1617, 1792, 1809
BsuTUI ATCGAT 1 cut(s) 1320
BtgZI GCGATG 2 cut(s) 525, 1643
BtsCI GGATG 5 cut(s) 445, 613, 1269, 1446, 1607
BtsIMutI CAGTG 4 cut(s) 310, 469, 837, 892
BtuMI TCGCGA 1 cut(s) 510
BveI ACCTGC 2 cut(s) 1150, 1687
Cac8I GCNNGC 1 cut(s) 1266
CfoI GCGC 1 cut(s) 1417
Cfr10I RCCGGY 2 cut(s) 1365, 1417
Cfr13I GGNCC 5 cut(s) 647, 1106, 1166, 1724, 1790
ClaI ATCGAT 1 cut(s) 1320
CseI GACGC 3 cut(s) 580, 929, 1334
CsiI ACCWGGT 1 cut(s) 214
Csp6I GTAC 8 cut(s) 66, 212, 225, 280, 481, 931, 1369, 1565
CspAI ACCGGT 1 cut(s) 1365
CviAII CATG 6 cut(s) 292, 992, 1249, 1587, 1763, 1778
CviQI GTAC 8 cut(s) 66, 212, 225, 280, 481, 931, 1369, 1565
DdeI CTNAG 2 cut(s) 1010, 1561
DinI GGCGCC 1 cut(s) 1416
DpnI GATC 6 cut(s) 78, 534, 687, 867, 1319, 1355
DpnII GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
DraIII CACNNNGTG 1 cut(s) 580
EaeI YGGCCR 3 cut(s) 414, 522, 1807
Eam1104I CTCTTC 2 cut(s) 191, 1575
EarI CTCTTC 2 cut(s) 191, 1575
EciI GGCGGA 1 cut(s) 1700
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 1742
Eco24I GRGCYC 2 cut(s) 207, 1081
Eco31I GGTCTC 1 cut(s) 261
Eco47I GGWCC 3 cut(s) 1106, 1166, 1724
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 4 cut(s) 131, 445, 1138, 1431
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 3 cut(s) 1106, 1166, 1724
EcoRII CCWGG 2 cut(s) 214, 1804
EcoT14I CCWWGG 1 cut(s) 1742
EcoT38I GRGCYC 2 cut(s) 207, 1081
EgeI GGCGCC 1 cut(s) 1416
EheI GGCGCC 1 cut(s) 1416
ErhI CCWWGG 1 cut(s) 1742
Esp3I CGTCTC 2 cut(s) 244, 799
FaeI CATG 6 cut(s) 295, 995, 1252, 1590, 1766, 1781
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 1823, 1855
FaqI GGGAC 3 cut(s) 583, 715, 973
FatI CATG 6 cut(s) 291, 991, 1248, 1586, 1762, 1777
FauNDI CATATG 1 cut(s) 871
FblI GTMKAC 2 cut(s) 90, 1636
Fnu4HI GCNGC 3 cut(s) 414, 1683, 1793
FokI GGATG 5 cut(s) 452, 620, 1256, 1453, 1594
FriOI GRGCYC 2 cut(s) 207, 1081
Fsp4HI GCNGC 3 cut(s) 414, 1683, 1793
FspBI CTAG 4 cut(s) 69, 101, 1265, 1889
GlaI GCGC 1 cut(s) 1416
GluI GCNGC 3 cut(s) 414, 1683, 1793
GsuI CTGGAG 1 cut(s) 1497
HaeII RGCGCY 1 cut(s) 1418
HaeIII GGCC 6 cut(s) 416, 524, 649, 1617, 1792, 1809
HgaI GACGC 3 cut(s) 580, 929, 1334
HhaI GCGC 1 cut(s) 1417
Hin1I GRCGYC 2 cut(s) 237, 1415
Hin1II CATG 6 cut(s) 295, 995, 1252, 1590, 1766, 1781
Hin6I GCGC 1 cut(s) 1415
HinP1I GCGC 1 cut(s) 1415
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 6 cut(s) 552, 779, 834, 1553, 1583, 1703
HphI GGTGA 7 cut(s) 257, 334, 802, 875, 1131, 1504, 1813
Hpy166II GTNNAC 4 cut(s) 91, 1183, 1637, 1768
Hpy188I TCNGA 5 cut(s) 14, 136, 316, 655, 1013
Hpy188III TCNNGA 9 cut(s) 55, 74, 233, 509, 549, 605, 1049, 1241, 1476
Hpy8I GTNNAC 4 cut(s) 91, 1183, 1637, 1768
Hpy99I CGWCG 5 cut(s) 61, 92, 191, 361, 913
HpyAV CCTTC 2 cut(s) 162, 1474
HpyCH4III ACNGT 3 cut(s) 841, 887, 904
HpyCH4IV ACGT 4 cut(s) 237, 657, 1099, 1404
HpyCH4V TGCA 6 cut(s) 156, 1257, 1289, 1410, 1785, 1864
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 761, 1254
HpyF3I CTNAG 2 cut(s) 1010, 1561
HpySE526I ACGT 4 cut(s) 237, 657, 1099, 1404
Hsp92I GRCGYC 2 cut(s) 237, 1415
Hsp92II CATG 6 cut(s) 295, 995, 1252, 1590, 1766, 1781
HspAI GCGC 1 cut(s) 1415
KasI GGCGCC 1 cut(s) 1414
Kpn2I TCCGGA 1 cut(s) 232
KpnI GGTACC 4 cut(s) 215, 228, 283, 484
Kzo9I GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
LmnI GCTCC 7 cut(s) 202, 316, 1084, 1478, 1595, 1687, 1826
LweI GCATC 5 cut(s) 39, 93, 400, 502, 903
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 4 cut(s) 69, 101, 1265, 1889
MaeII ACGT 4 cut(s) 237, 657, 1099, 1404
MaeIII GTNAC 4 cut(s) 245, 340, 1420, 1819
MalI GATC 6 cut(s) 78, 534, 687, 867, 1319, 1355
MboI GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
MboII GAAGA 7 cut(s) 208, 448, 451, 1437, 1507, 1517, 1592
MflI RGATCY 2 cut(s) 532, 1353
MhlI GDGCHC 3 cut(s) 207, 1081, 1483
MlsI TGGCCA 1 cut(s) 1809
MluCI AATT 7 cut(s) 26, 370, 563, 709, 1172, 1573, 1641
MluNI TGGCCA 1 cut(s) 1809
Mly113I GGCGCC 1 cut(s) 1415
MlyI GAGTC 2 cut(s) 1547, 1592
MmeI TCCRAC 4 cut(s) 294, 1183, 1287, 1303
Mox20I TGGCCA 1 cut(s) 1809
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 2 cut(s) 894, 1394
MscI TGGCCA 1 cut(s) 1809
MseI TTAA 8 cut(s) 252, 621, 660, 671, 801, 960, 1454, 1883
MslI CAYNNNNRTG 3 cut(s) 581, 1226, 1780
Msp20I TGGCCA 1 cut(s) 1809
MspR9I CCNGG 5 cut(s) 216, 346, 972, 1648, 1806
Mva1269I GAATGC 3 cut(s) 764, 1233, 1630
MvaI CCWGG 2 cut(s) 216, 1806
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 521, 761, 1254
NarI GGCGCC 1 cut(s) 1415
NciI CCSGG 3 cut(s) 346, 972, 1648
NdeI CATATG 1 cut(s) 871
NdeII GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
NheI GCTAGC 1 cut(s) 1264
NlaIII CATG 6 cut(s) 295, 995, 1252, 1590, 1766, 1781
NmuCI GTSAC 3 cut(s) 245, 340, 1819
NruI TCGCGA 1 cut(s) 510
NspI RCATGY 3 cut(s) 295, 1766, 1781
PaqCI CACCTGC 1 cut(s) 1150
PciI ACATGT 2 cut(s) 291, 1777
PctI GAATGC 3 cut(s) 764, 1233, 1630
PdmI GAANNNNTTC 2 cut(s) 894, 1394
PfeI GAWTC 4 cut(s) 552, 779, 834, 1703
PfoI TCCNGGA 1 cut(s) 1646
PinAI ACCGGT 1 cut(s) 1365
PkrI GCNGC 3 cut(s) 415, 1684, 1794
PleI GAGTC 2 cut(s) 1547, 1591
PluTI GGCGCC 1 cut(s) 1418
PpsI GAGTC 2 cut(s) 1547, 1591
PpuMI RGGWCCY 3 cut(s) 1106, 1166, 1724
PscI ACATGT 2 cut(s) 291, 1777
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 3 cut(s) 1106, 1166, 1724
Psp6I CCWGG 2 cut(s) 214, 1804
PspGI CCWGG 2 cut(s) 214, 1804
PspPI GGNCC 5 cut(s) 647, 1106, 1166, 1724, 1790
PspPPI RGGWCCY 3 cut(s) 1106, 1166, 1724
PsuI RGATCY 2 cut(s) 532, 1353
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 8 cut(s) 67, 213, 226, 281, 482, 932, 1370, 1566
RsaNI GTAC 8 cut(s) 66, 212, 225, 280, 481, 931, 1369, 1565
RseI CAYNNNNRTG 3 cut(s) 581, 1226, 1780
SacI GAGCTC 1 cut(s) 207
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 8 cut(s) 252, 621, 660, 671, 801, 960, 1454, 1883
SatI GCNGC 3 cut(s) 414, 1683, 1793
Sau3AI GATC 6 cut(s) 76, 532, 685, 865, 1317, 1353
Sau96I GGNCC 5 cut(s) 647, 1106, 1166, 1724, 1790
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 2 cut(s) 1547, 1592
ScrFI CCNGG 5 cut(s) 216, 346, 972, 1648, 1806
SduI GDGCHC 3 cut(s) 207, 1081, 1483
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 5 cut(s) 39, 93, 400, 502, 903
SfcI CTRYAG 1 cut(s) 1026
SfoI GGCGCC 1 cut(s) 1416
SinI GGWCC 3 cut(s) 1106, 1166, 1724
SmiMI CAYNNNNRTG 3 cut(s) 581, 1226, 1780
SmlI CTYRAG 1 cut(s) 1241
SmoI CTYRAG 1 cut(s) 1241
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 7 cut(s) 26, 370, 563, 709, 1172, 1573, 1641
SsiI CCGC 6 cut(s) 413, 785, 816, 1682, 1685, 1793
SspDI GGCGCC 1 cut(s) 1414
SspMI CTAG 4 cut(s) 69, 101, 1265, 1889
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 5 cut(s) 214, 344, 970, 1646, 1804
StyI CCWWGG 1 cut(s) 1742
TaaI ACNGT 3 cut(s) 841, 887, 904
TaiI ACGT 4 cut(s) 240, 660, 1102, 1407
TaqI TCGA 7 cut(s) 75, 90, 123, 335, 911, 1320, 1706
TasI AATT 7 cut(s) 26, 370, 563, 709, 1172, 1573, 1641
TatI WGTACW 3 cut(s) 65, 930, 1564
TauI GCSGC 3 cut(s) 416, 1685, 1795
TfiI GAWTC 4 cut(s) 552, 779, 834, 1703
Tru1I TTAA 8 cut(s) 252, 621, 660, 671, 801, 960, 1454, 1883
Tru9I TTAA 8 cut(s) 252, 621, 660, 671, 801, 960, 1454, 1883
TscAI CASTG 4 cut(s) 310, 469, 844, 892
TseFI GTSAC 3 cut(s) 245, 340, 1819
Tsp45I GTSAC 3 cut(s) 245, 340, 1819
TspDTI ATGAA 7 cut(s) 439, 458, 516, 544, 928, 1076, 1265
TspGWI ACGGA 4 cut(s) 302, 345, 781, 897
TspRI CASTG 4 cut(s) 310, 469, 844, 892
VpaK11BI GGWCC 3 cut(s) 1106, 1166, 1724
XapI RAATTY 1 cut(s) 563
XceI RCATGY 3 cut(s) 295, 1766, 1781
XmiI GTMKAC 2 cut(s) 90, 1636
XmnI GAANNNNTTC 2 cut(s) 894, 1394
XspI CTAG 4 cut(s) 69, 101, 1265, 1889
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.