Rmu_co8443405.1_g000001

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8443405.1
OG0014090
Physical Location & Seq
Forward (+)
1 .. 1447
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8443405.1_g000001.1.cds

Sequence Viewer

Length: 887 bp
acaagcctcctatagctgatttgccctactttcttgaatttcaagtatcacacaaagtatgggctccccttcacaatgacctacgtttaggtcctgccacaaatagcaaatctaatccatcatcacctgctattcacttcagcttgatgggtcctaggttatatgtaaacaccacacaggttagagttggaaataatgcccctgtcacagggatgcgcttgtttcttgagggcatgaaatgcaacaggctagccatccacctccaacatctagcgaacactccattgatgttggagaacaagatcgatgacacaacgatgtggcgaggatcagaagagatcgacgatgaccggtactttgaaccaacaaacgggaaaaagttctcccacgtgtgcacggcgccagtaacgtacgacccaaaatggatatcttcgggcataacctcggccttcattgtctcgggagcacaacttgtggtcaagcaacacggctcaaagaatgttctccatcttcggctcttgttctccaaagtcttggactctttcttagtgcaatcaaattgggcagagtcatgttcttcaactaaatcgtcgtcgtctggccttttgaatgcgatgagtagaccaatttctgggaactattggggaaaagagaaagtagaacctgccgcagaggatgtgatattggattcgagtgtttttcctgcagggcctccggtgcctttgccaaggcaaaaatttgtgaagcatgtaaacacctcacatgtatgcaaagggccgcaggatagtcctggccattggttggtcaccggagcaaagctcaacttggtcaagggaaagattggcttgcaagtcaagttctctctgttaaacctagcagaaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

294

Amino Acids

32.4

Weight (kDa)

9.38

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 135
Acc36I ACCTGC 2 cut(s) 135, 672
AccB1I GGYRCC 2 cut(s) 399, 717
AccB7I CCANNNNNTGG 2 cut(s) 631, 801
AccI GTMKAC 1 cut(s) 621
AciI CCGC 2 cut(s) 668, 778
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 2 cut(s) 37, 736
AcuI CTGAAG 1 cut(s) 123
AcvI CACGTG 1 cut(s) 390
AcyI GRCGYC 1 cut(s) 400
AfaI GTAC 2 cut(s) 355, 412
AfiI CCNNNNNNNGG 4 cut(s) 208, 370, 631, 801
AflIII ACRYGT 2 cut(s) 389, 762
AgeI ACCGGT 1 cut(s) 350
AgsI TTSAA 5 cut(s) 37, 43, 361, 581, 609
AjnI CCWGG 1 cut(s) 789
AluBI AGCT 3 cut(s) 16, 143, 819
AluI AGCT 3 cut(s) 16, 143, 819
Alw21I GWGCWC 2 cut(s) 397, 468
Alw26I GTCTC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 459
AoxI GGCC 5 cut(s) 446, 600, 709, 775, 792
ApaLI GTGCAC 1 cut(s) 393
ApoI RAATTY 2 cut(s) 37, 736
ArsI GACNNNNNNTTYG 2 cut(s) 340, 372
AsiGI ACCGGT 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 379
AspA2I CCTAGG 1 cut(s) 154
AspLEI GCGC 2 cut(s) 218, 402
AspS9I GGNCC 4 cut(s) 91, 151, 709, 775
AsuHPI GGTGA 2 cut(s) 116, 798
AsuNHI GCTAGC 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 459
AvaII GGWCC 2 cut(s) 91, 151
AvrII CCTAGG 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 397
BalI TGGCCA 1 cut(s) 794
BanI GGYRCC 2 cut(s) 399, 717
BanII GRGCYC 1 cut(s) 66
BbrPI CACGTG 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 397, 468
BccI CCATC 4 cut(s) 126, 141, 262, 515
BceAI ACGGC 2 cut(s) 413, 504
BciT130I CCWGG 1 cut(s) 791
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 4 cut(s) 155, 250, 271, 874
BfmI CTRYAG 2 cut(s) 11, 704
BfoI RGCGCY 1 cut(s) 403
BfuAI ACCTGC 2 cut(s) 135, 672
BisI GCNGC 2 cut(s) 668, 778
BlnI CCTAGG 1 cut(s) 154
BlsI GCNGC 2 cut(s) 669, 779
Bme1390I CCNGG 1 cut(s) 791
Bme18I GGWCC 2 cut(s) 91, 151
BmeT110I CYCGRG 1 cut(s) 459
BmgT120I GGNCC 4 cut(s) 91, 151, 709, 775
BmiI GGNNCC 4 cut(s) 65, 152, 401, 719
BmrFI CCNGG 1 cut(s) 791
BmsI GCATC 1 cut(s) 203
BmtI GCTAGC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 803, 835
BpuEI CTTGAG 1 cut(s) 247
Bsa29I ATCGAT 1 cut(s) 305
BsaAI YACGTR 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 154, 443, 727
BsaWI WCCGGW 3 cut(s) 350, 714, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 370, 631, 801
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 1 cut(s) 403
BseBI CCWGG 1 cut(s) 791
BseCI ATCGAT 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 154, 443, 727
BseGI GGATG 3 cut(s) 218, 254, 681
BseLI CCNNNNNNNGG 4 cut(s) 208, 370, 631, 801
BseNI ACTGG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 397
BshFI GGCC 5 cut(s) 448, 602, 711, 777, 794
BshNI GGYRCC 2 cut(s) 399, 717
BshTI ACCGGT 1 cut(s) 350
BshVI ATCGAT 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 397, 468
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 3 cut(s) 351, 715, 809
BsiWI CGTACG 1 cut(s) 410
BslI CCNNNNNNNGG 4 cut(s) 208, 370, 631, 801
BsmAI GTCTC 1 cut(s) 462
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 5 cut(s) 448, 602, 711, 777, 794
BsoBI CYCGRG 1 cut(s) 459
Bsp1286I GDGCHC 3 cut(s) 66, 397, 468
Bsp143I GATC 3 cut(s) 302, 328, 338
BspACI CCGC 2 cut(s) 668, 778
BspANI GGCC 5 cut(s) 448, 602, 711, 777, 794
BspDI ATCGAT 1 cut(s) 305
BspLI GGNNCC 4 cut(s) 65, 152, 401, 719
BspMAI CTGCAG 1 cut(s) 708
BspMI ACCTGC 2 cut(s) 135, 672
BspOI GCTAGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 2 cut(s) 399, 717
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 154, 443, 727
BssMI GATC 3 cut(s) 302, 328, 338
BssNI GRCGYC 1 cut(s) 400
BssT1I CCWWGG 2 cut(s) 154, 727
Bst2UI CCWGG 1 cut(s) 791
Bst6I CTCTTC 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 251, 847
BstDEI CTNAG 1 cut(s) 546
BstEII GGTNACC 1 cut(s) 804
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 218, 254, 681
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 2 cut(s) 218, 402
BstKTI GATC 3 cut(s) 305, 331, 341
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 3 cut(s) 302, 328, 338
BstMWI GCNNNNNNNGC 2 cut(s) 239, 717
BstNI CCWGG 1 cut(s) 791
BstNSI RCATGY 2 cut(s) 751, 766
BstPI GGTNACC 1 cut(s) 804
BstSCI CCNGG 1 cut(s) 789
BstSFI CTRYAG 2 cut(s) 11, 704
BstSLI GKGCMC 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 534
Bsu15I ATCGAT 1 cut(s) 305
BsuRI GGCC 5 cut(s) 448, 602, 711, 777, 794
BsuTUI ATCGAT 1 cut(s) 305
BtgZI GCGATG 1 cut(s) 628
BtsCI GGATG 3 cut(s) 218, 254, 681
BveI ACCTGC 2 cut(s) 135, 672
Cac8I GCNNGC 2 cut(s) 251, 847
CfoI GCGC 2 cut(s) 218, 402
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 4 cut(s) 91, 151, 709, 775
ClaI ATCGAT 1 cut(s) 305
Csp6I GTAC 2 cut(s) 354, 411
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 4 cut(s) 234, 572, 748, 763
CviQI GTAC 2 cut(s) 354, 411
DdeI CTNAG 1 cut(s) 546
DinI GGCGCC 1 cut(s) 401
DpnI GATC 3 cut(s) 304, 330, 340
DpnII GATC 3 cut(s) 302, 328, 338
EaeI YGGCCR 1 cut(s) 792
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 154, 727
Eco24I GRGCYC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 91, 151
Eco57I CTGAAG 1 cut(s) 123
Eco72I CACGTG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 459
Eco91I GGTNACC 1 cut(s) 804
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 3 cut(s) 91, 151, 709
EcoO65I GGTNACC 1 cut(s) 804
EcoRII CCWGG 1 cut(s) 789
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 154, 727
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 401
EheI GGCGCC 1 cut(s) 401
ErhI CCWWGG 2 cut(s) 154, 727
FaeI CATG 4 cut(s) 237, 575, 751, 766
FalI AAGNNNNNCTT 4 cut(s) 808, 840, 829, 861
FatI CATG 4 cut(s) 233, 571, 747, 762
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 2 cut(s) 668, 778
FokI GGATG 3 cut(s) 225, 241, 688
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 668, 778
FspBI CTAG 4 cut(s) 155, 250, 271, 874
GlaI GCGC 2 cut(s) 217, 401
GluI GCNGC 2 cut(s) 668, 778
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 5 cut(s) 448, 602, 711, 777, 794
HapII CCGG 3 cut(s) 351, 715, 809
HhaI GCGC 2 cut(s) 218, 402
Hin1I GRCGYC 1 cut(s) 400
Hin1II CATG 4 cut(s) 237, 575, 751, 766
Hin6I GCGC 2 cut(s) 216, 400
HinP1I GCGC 2 cut(s) 216, 400
HinfI GANTC 3 cut(s) 538, 568, 688
HpaII CCGG 3 cut(s) 351, 715, 809
HphI GGTGA 2 cut(s) 116, 798
Hpy166II GTNNAC 4 cut(s) 168, 395, 622, 753
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 3 cut(s) 34, 226, 461
Hpy8I GTNNAC 4 cut(s) 168, 395, 622, 753
Hpy99I CGWCG 3 cut(s) 346, 594, 597
HpyAV CCTTC 2 cut(s) 79, 459
HpyCH4IV ACGT 3 cut(s) 84, 389, 409
HpyCH4V TGCA 6 cut(s) 242, 395, 552, 706, 770, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 717
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 3 cut(s) 84, 389, 409
Hsp92I GRCGYC 1 cut(s) 400
Hsp92II CATG 4 cut(s) 237, 575, 751, 766
HspAI GCGC 2 cut(s) 216, 400
KasI GGCGCC 1 cut(s) 399
Kzo9I GATC 3 cut(s) 302, 328, 338
LmnI GCTCC 3 cut(s) 69, 463, 811
LweI GCATC 1 cut(s) 203
MaeI CTAG 4 cut(s) 155, 250, 271, 874
MaeII ACGT 3 cut(s) 84, 389, 409
MaeIII GTNAC 3 cut(s) 204, 405, 804
MalI GATC 3 cut(s) 304, 330, 340
MboI GATC 3 cut(s) 302, 328, 338
MboII GAAGA 4 cut(s) 346, 422, 502, 569
MhlI GDGCHC 3 cut(s) 66, 397, 468
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 4 cut(s) 37, 558, 626, 736
MluNI TGGCCA 1 cut(s) 794
Mly113I GGCGCC 1 cut(s) 400
MlyI GAGTC 2 cut(s) 532, 577
MmeI TCCRAC 3 cut(s) 168, 272, 288
MnlI CCTC 8 cut(s) 17, 222, 271, 319, 453, 666, 722, 768
Mox20I TGGCCA 1 cut(s) 794
MroXI GAANNNNTTC 1 cut(s) 379
MscI TGGCCA 1 cut(s) 794
MseI TTAA 1 cut(s) 868
MslI CAYNNNNRTG 3 cut(s) 211, 316, 765
Msp20I TGGCCA 1 cut(s) 794
MspI CCGG 3 cut(s) 351, 715, 809
MspR9I CCNGG 1 cut(s) 791
Mva1269I GAATGC 1 cut(s) 615
MvaI CCWGG 1 cut(s) 791
MwoI GCNNNNNNNGC 2 cut(s) 239, 717
NarI GGCGCC 1 cut(s) 400
NdeII GATC 3 cut(s) 302, 328, 338
NheI GCTAGC 1 cut(s) 249
NlaIII CATG 4 cut(s) 237, 575, 751, 766
NlaIV GGNNCC 4 cut(s) 65, 152, 401, 719
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 204, 804
NspI RCATGY 2 cut(s) 751, 766
PaqCI CACCTGC 1 cut(s) 135
PciI ACATGT 1 cut(s) 762
PctI GAATGC 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 688
Pfl23II CGTACG 1 cut(s) 410
PflMI CCANNNNNTGG 2 cut(s) 631, 801
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 2 cut(s) 669, 779
PleI GAGTC 2 cut(s) 532, 576
PluTI GGCGCC 1 cut(s) 403
PmaCI CACGTG 1 cut(s) 390
PmlI CACGTG 1 cut(s) 390
PpsI GAGTC 2 cut(s) 532, 576
Ppu21I YACGTR 1 cut(s) 390
PpuMI RGGWCCY 2 cut(s) 91, 151
PscI ACATGT 1 cut(s) 762
Psp5II RGGWCCY 2 cut(s) 91, 151
Psp6I CCWGG 1 cut(s) 789
PspCI CACGTG 1 cut(s) 390
PspEI GGTNACC 1 cut(s) 804
PspGI CCWGG 1 cut(s) 789
PspLI CGTACG 1 cut(s) 410
PspN4I GGNNCC 4 cut(s) 65, 152, 401, 719
PspPI GGNCC 4 cut(s) 91, 151, 709, 775
PspPPI RGGWCCY 2 cut(s) 91, 151
PstI CTGCAG 1 cut(s) 708
RsaI GTAC 2 cut(s) 355, 412
RsaNI GTAC 2 cut(s) 354, 411
RseI CAYNNNNRTG 3 cut(s) 211, 316, 765
SaqAI TTAA 1 cut(s) 868
SatI GCNGC 2 cut(s) 668, 778
Sau3AI GATC 3 cut(s) 302, 328, 338
Sau96I GGNCC 4 cut(s) 91, 151, 709, 775
SbfI CCTGCAGG 1 cut(s) 708
SchI GAGTC 2 cut(s) 532, 577
ScrFI CCNGG 1 cut(s) 791
SdaI CCTGCAGG 1 cut(s) 708
SduI GDGCHC 3 cut(s) 66, 397, 468
SfaNI GCATC 1 cut(s) 203
SfcI CTRYAG 2 cut(s) 11, 704
SfoI GGCGCC 1 cut(s) 401
SinI GGWCC 2 cut(s) 91, 151
SmiMI CAYNNNNRTG 3 cut(s) 211, 316, 765
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse8387I CCTGCAGG 1 cut(s) 708
Sse9I AATT 4 cut(s) 37, 558, 626, 736
SsiI CCGC 2 cut(s) 668, 778
SspDI GGCGCC 1 cut(s) 399
SspMI CTAG 4 cut(s) 155, 250, 271, 874
StyD4I CCNGG 1 cut(s) 789
StyI CCWWGG 2 cut(s) 154, 727
TaiI ACGT 3 cut(s) 87, 392, 412
TaqI TCGA 3 cut(s) 305, 341, 691
TasI AATT 4 cut(s) 37, 558, 626, 736
TauI GCSGC 2 cut(s) 670, 780
TfiI GAWTC 1 cut(s) 688
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TseFI GTSAC 2 cut(s) 204, 804
Tsp45I GTSAC 2 cut(s) 204, 804
TspDTI ATGAA 2 cut(s) 250, 441
Van91I CCANNNNNTGG 2 cut(s) 631, 801
VneI GTGCAC 1 cut(s) 393
VpaK11BI GGWCC 2 cut(s) 91, 151
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 2 cut(s) 37, 736
XceI RCATGY 2 cut(s) 751, 766
XmaJI CCTAGG 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 379
XspI CTAG 4 cut(s) 155, 250, 271, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.