MD13G1019100.v1.1

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014090
Physical Location & Seq
Forward (+)
1203699 .. 1208780
5082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1019100.v1.1.491

Sequence Viewer

Length: 1890 bp
ATGAGTGGTGACGATCACAGCATGATCGTGGAGAGGGCACTAAACAGCCTAGGCAAAGGATTTGATTTGACGTCAGATTTCCGACTGAAATACTGCAAAGGAGGCAAACAAAGATTGGTTGTGCTCAACGACACCGATAAAAGAGAACTCACAGTTCCTGGTTTCAGAACCATTCAGAACGTCTCCGTCGACATTAAATGTGACAAAGGCGACCGTACTCGTTACCAATCCGATATCCTCACCTTCAATCAGATGTCGGAGTTTTTCAACCAAAGGTCGAGTGTGCCGGGGAAAATCCCATCAGGGCTGTTCAACGCTATGTTTGGATTCGACAGCGGTTCGTGGGCAAAGGATGCAGCAAACATCAAGCACTTGGCTCTTGATGGCTACTACATCATATTCTTCAATGTTCATATTGATAGATACCCTCTTGTTCTCGCCGACGAAGTCCGTGAGTCCGTTCCATCCACCTGGGATCCTCCTGCCCTTGCAAGATTCATTGAGAAATATGGCACTCACATCGTCGTGGGATTGAGTGTTGGGGGCGAGGATGTTGTCTTAGTAAAGCAAGATAAATCTTCAAACATGCAGCCATCGGACCTCAAAAATCACTTGAATGAGCTTGGAGATCAGCAATTTACAGGGGCATGCAGTTTCTCCCCATCACACTCCAAATCCAAAGACCAAAAACACAAGGCACCACAGACAATCAGTGTCTTTCATGATCCTCAGTTGGCCATTAATGGCTTCTCAACCACAAAATCAAAAGATGGTATTAGTGTGATATTCTATAAAAAAGGAGGCGATCTGCTGGCCAGCAGCCACTACGAGTGGCTTCTCACAGCTCCATCAATGCCGGACGCAATTCAGTTTAGTTTCATTCCGATCACTTCTCTGCTCAAAGATGTGCCAGGCAAGGGTTTCCTGTCGCATGCCATCAACCTCTATCTTCGATACAAGCCTCCAATAGATGATCTGCAGTACTTTCTGGACTTTCAAACCCACAAACTTTGGGCTCCTGTTCTCAATGGGTTGGCTCTAGGCCCAGCCTCCAATAGGAAGACTCGGTCCCCTGCAATCCACGTCAACCGGATGGGTCCTAAACTATATGTAAACTCTGCGCAAGTTAGGGTTGCATTTAGGCCTGTCACGGGGATGCGCTTGTATCTTGAGGGCAGGAAAGCCAACAGGCTAGCCGTCCACCTCCAACATCTATCAAACACACCATTCATGTTGCAAAACAAGATAGATGACACACCAACATGGCGAGGATCGGATGAAATTGCCGACGACCGCTACTTTGAAGCCATAAACCGGAAAAAGATTTCCCATGTGTGCACTGTACCGGTAAAATACGACCCCAAATGGATGTCGTCCAAGAAAGATGCGTCCTTCATAGTGTCCGGGGTCCAGCTTGTTGTAAAGAAGCATGACACAAAGAATGTCCTCCATCTTCGGCTCTTGTTCTCCAAAGTCTCAGATCATTTCGTTGTCCAATCAAATTGGACTGAACCACCGTCGGAACATTCCCAAAAGTCGTCATCGTCCGGTCTCTTCAGTGCCATAAGTACATCGATTTCGGGGAGTAATTGGTCGAGAGATAGAGTACTGCCCGATGATGATCGAGTGATTGTGGATTCGAGTGTGTTCCCGACAGGGCCTCCGGTGCCGCTGCAGTTACAAAAGTTTGTGAAGTACGTGGACATGTCACATCTGTGTAGGGGCCCACAAGACGGTCCAGGGTATTGGTTGGTCACTGGAGCTAGGCTCAACTTAGTGAAGGGGAAGATAAGTTTGCAAGTCAAGTTCTCTTTGTTAAACTTATGTTCACCATCACCATCAGTCACCCTCCGAGATTTGATGTACAACGTTGTGGATTTGGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

630

Amino Acids

70.38

Weight (kDa)

9.23

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 103 - 316 8e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 74
Acc16I TGCGCA 1 cut(s) 1122
AccB1I GGYRCC 2 cut(s) 697, 1666
AccI GTMKAC 1 cut(s) 189
AciI CCGC 3 cut(s) 336, 1294, 1670
AclI AACGTT 1 cut(s) 1869
AclWI GGATC 4 cut(s) 470, 483, 719, 1279
AcoI YGGCCR 2 cut(s) 735, 813
AcuI CTGAAG 1 cut(s) 1540
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 7 cut(s) 217, 983, 1344, 1570, 1608, 1697, 1865
AfiI CCNNNNNNNGG 5 cut(s) 917, 1056, 1151, 1314, 1733
AflIII ACRYGT 1 cut(s) 1704
AgeI ACCGGT 1 cut(s) 1345
AgsI TTSAA 8 cut(s) 247, 268, 313, 406, 582, 616, 998, 1304
AjiI CACGTC 1 cut(s) 1084
AjnI CCWGG 4 cut(s) 157, 470, 910, 1738
AleI CACNNNNGTG 1 cut(s) 1714
AluBI AGCT 4 cut(s) 622, 845, 1414, 1763
AluI AGCT 4 cut(s) 622, 845, 1414, 1763
Alw21I GWGCWC 2 cut(s) 126, 1340
Alw26I GTCTC 3 cut(s) 187, 1480, 1556
Alw44I GTGCAC 1 cut(s) 1336
AlwI GGATC 4 cut(s) 470, 483, 719, 1279
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 6 cut(s) 735, 813, 1042, 1142, 1658, 1723
ApaI GGGCCC 1 cut(s) 1727
ApaLI GTGCAC 1 cut(s) 1336
ApeKI GCWGC 4 cut(s) 356, 589, 819, 1672
ArsI GACNNNNNNTTYG 2 cut(s) 1283, 1315
AseI ATTAAT 1 cut(s) 741
AsiGI ACCGGT 1 cut(s) 1345
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 2 cut(s) 1123, 1161
AspS9I GGNCC 9 cut(s) 598, 1043, 1068, 1097, 1408, 1658, 1723, 1724, 1736
AsuC2I CCSGG 2 cut(s) 288, 1405
AsuHPI GGTGA 5 cut(s) 20, 232, 1821, 1827, 1837
AsuNHI GCTAGC 1 cut(s) 1192
AvaII GGWCC 5 cut(s) 598, 1068, 1097, 1408, 1736
AvrII CCTAGG 1 cut(s) 49
BaeGI GKGCMC 3 cut(s) 40, 1340, 1727
BalI TGGCCA 2 cut(s) 737, 815
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 2 cut(s) 697, 1666
BanII GRGCYC 2 cut(s) 1018, 1727
BbsI GAAGAC 1 cut(s) 1067
Bbv12I GWGCWC 2 cut(s) 126, 1340
BbvI GCAGC 4 cut(s) 368, 601, 831, 1659
BceAI ACGGC 1 cut(s) 1181
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 4 cut(s) 159, 472, 912, 1740
BcnI CCSGG 2 cut(s) 288, 1405
BcoDI GTCTC 3 cut(s) 187, 1480, 1556
BfaI CTAG 4 cut(s) 50, 1040, 1193, 1764
BfmI CTRYAG 2 cut(s) 977, 1673
BisI GCNGC 5 cut(s) 357, 590, 820, 1670, 1673
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 5 cut(s) 358, 591, 821, 1671, 1674
BmcAI AGTACT 2 cut(s) 983, 1608
Bme1390I CCNGG 6 cut(s) 159, 288, 472, 912, 1405, 1740
Bme18I GGWCC 5 cut(s) 598, 1068, 1097, 1408, 1736
BmgBI CACGTC 1 cut(s) 1084
BmgT120I GGNCC 9 cut(s) 598, 1043, 1068, 1097, 1408, 1658, 1723, 1724, 1736
BmiI GGNNCC 9 cut(s) 477, 699, 1017, 1070, 1098, 1409, 1668, 1724, 1725
BmrFI CCNGG 6 cut(s) 159, 288, 472, 912, 1405, 1740
BmsI GCATC 3 cut(s) 343, 1146, 1375
BmtI GCTAGC 1 cut(s) 1196
BpiI GAAGAC 1 cut(s) 1067
BplI GAGNNNNNCTC 2 cut(s) 1752, 1784
BpmI CTGGAG 1 cut(s) 1779
BpuEI CTTGAG 1 cut(s) 1190
BpuMI CCSGG 2 cut(s) 288, 1405
Bsa29I ATCGAT 1 cut(s) 1574
BsaAI YACGTR 1 cut(s) 1699
BsaBI GATNNNNATC 1 cut(s) 1620
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 1556
BsaJI CCNNGG 5 cut(s) 49, 287, 471, 1404, 1739
BsaWI WCCGGW 5 cut(s) 1089, 1314, 1345, 1547, 1663
BsaXI ACNNNNNCTCC 2 cut(s) 1645, 1675
Bsc4I CCNNNNNNNGG 5 cut(s) 917, 1056, 1151, 1314, 1733
Bse118I RCCGGY 1 cut(s) 1345
Bse1I ACTGG 1 cut(s) 1762
Bse8I GATNNNNATC 1 cut(s) 1620
BseBI CCWGG 4 cut(s) 159, 472, 912, 1740
BseCI ATCGAT 1 cut(s) 1574
BseDI CCNNGG 5 cut(s) 49, 287, 471, 1404, 1739
BseGI GGATG 7 cut(s) 358, 464, 556, 1098, 1161, 1282, 1374
BseJI GATNNNNATC 1 cut(s) 1620
BseLI CCNNNNNNNGG 5 cut(s) 917, 1056, 1151, 1314, 1733
BseMII CTCAG 2 cut(s) 743, 1491
BseNI ACTGG 1 cut(s) 1762
BseSI GKGCMC 3 cut(s) 40, 1340, 1727
BseXI GCAGC 4 cut(s) 368, 601, 831, 1659
BseYI CCCAGC 1 cut(s) 1045
Bsh1285I CGRYCG 2 cut(s) 214, 1294
BshFI GGCC 6 cut(s) 737, 815, 1044, 1144, 1660, 1725
BshNI GGYRCC 2 cut(s) 697, 1666
BshTI ACCGGT 1 cut(s) 1345
BshVI ATCGAT 1 cut(s) 1574
BsiEI CGRYCG 2 cut(s) 214, 1294
BsiHKAI GWGCWC 2 cut(s) 126, 1340
BsiSI CCGG 8 cut(s) 287, 857, 1090, 1315, 1346, 1404, 1548, 1664
BslFI GGGAC 1 cut(s) 1054
BslI CCNNNNNNNGG 5 cut(s) 917, 1056, 1151, 1314, 1733
BsmAI GTCTC 3 cut(s) 187, 1480, 1556
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 1054
BsnI GGCC 6 cut(s) 737, 815, 1044, 1144, 1660, 1725
Bso31I GGTCTC 1 cut(s) 1556
Bsp120I GGGCCC 1 cut(s) 1723
Bsp1286I GDGCHC 5 cut(s) 40, 126, 1018, 1340, 1727
Bsp1407I TGTACA 1 cut(s) 1863
BspACI CCGC 3 cut(s) 336, 1294, 1670
BspANI GGCC 6 cut(s) 737, 815, 1044, 1144, 1660, 1725
BspCNI CTCAG 2 cut(s) 742, 1490
BspDI ATCGAT 1 cut(s) 1574
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 9 cut(s) 477, 699, 1017, 1070, 1098, 1409, 1668, 1724, 1725
BspMAI CTGCAG 2 cut(s) 981, 1677
BspOI GCTAGC 1 cut(s) 1196
BspPI GGATC 4 cut(s) 470, 483, 719, 1279
BspT107I GGYRCC 2 cut(s) 697, 1666
BspTNI GGTCTC 1 cut(s) 1556
BsrFI RCCGGY 1 cut(s) 1345
BsrGI TGTACA 1 cut(s) 1863
BsrI ACTGG 1 cut(s) 1762
BssAI RCCGGY 1 cut(s) 1345
BssECI CCNNGG 5 cut(s) 49, 287, 471, 1404, 1739
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 4 cut(s) 159, 472, 912, 1740
Bst4CI ACNGT 5 cut(s) 154, 215, 1342, 1518, 1736
Bst6I CTCTTC 1 cut(s) 1559
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 1863
BstBAI YACGTR 1 cut(s) 1699
BstC8I GCNNGC 5 cut(s) 649, 813, 817, 933, 1194
BstDEI CTNAG 4 cut(s) 559, 729, 1477, 1774
BstENI CCTNNNNNAGG 1 cut(s) 1054
BstF5I GGATG 7 cut(s) 358, 464, 556, 1098, 1161, 1282, 1374
BstHHI GCGC 2 cut(s) 1123, 1161
BstMAI GTCTC 3 cut(s) 187, 1480, 1556
BstMCI CGRYCG 2 cut(s) 214, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 102, 353, 1666
BstNI CCWGG 4 cut(s) 159, 472, 912, 1740
BstNSI RCATGY 4 cut(s) 589, 651, 935, 1708
BstSCI CCNGG 6 cut(s) 157, 286, 470, 910, 1403, 1738
BstSFI CTRYAG 2 cut(s) 977, 1673
BstSLI GKGCMC 3 cut(s) 40, 1340, 1727
BstV1I GCAGC 4 cut(s) 368, 601, 831, 1659
BstV2I GAAGAC 1 cut(s) 1067
BstX2I RGATCY 1 cut(s) 475
BstXI CCANNNNNNTGG 2 cut(s) 471, 1746
BstYI RGATCY 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 1574
BsuRI GGCC 6 cut(s) 737, 815, 1044, 1144, 1660, 1725
BsuTUI ATCGAT 1 cut(s) 1574
BtrI CACGTC 1 cut(s) 1084
BtsCI GGATG 7 cut(s) 358, 464, 556, 1098, 1161, 1282, 1374
BtsIMutI CAGTG 4 cut(s) 718, 1338, 1564, 1755
Cac8I GCNNGC 5 cut(s) 649, 813, 817, 933, 1194
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 1123, 1161
Cfr10I RCCGGY 1 cut(s) 1345
Cfr13I GGNCC 9 cut(s) 598, 1043, 1068, 1097, 1408, 1658, 1723, 1724, 1736
ClaI ATCGAT 1 cut(s) 1574
CseI GACGC 2 cut(s) 869, 1377
Csp6I GTAC 7 cut(s) 216, 982, 1343, 1569, 1607, 1696, 1864
CspAI ACCGGT 1 cut(s) 1345
CspCI CAANNNNNGTGG 2 cut(s) 992, 1027
CviQI GTAC 7 cut(s) 216, 982, 1343, 1569, 1607, 1696, 1864
DdeI CTNAG 4 cut(s) 559, 729, 1477, 1774
EaeI YGGCCR 2 cut(s) 735, 813
Eam1104I CTCTTC 1 cut(s) 1559
EarI CTCTTC 1 cut(s) 1559
Eco130I CCWWGG 1 cut(s) 49
Eco147I AGGCCT 1 cut(s) 1144
Eco24I GRGCYC 2 cut(s) 1018, 1727
Eco31I GGTCTC 1 cut(s) 1556
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 5 cut(s) 598, 1068, 1097, 1408, 1736
Eco57I CTGAAG 1 cut(s) 1540
EcoNI CCTNNNNNAGG 1 cut(s) 1054
EcoO109I RGGNCCY 3 cut(s) 1097, 1658, 1723
EcoRII CCWGG 4 cut(s) 157, 470, 910, 1738
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 1018, 1727
ErhI CCWWGG 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 187
FaqI GGGAC 1 cut(s) 1054
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 357, 590, 820, 1670, 1673
FokI GGATG 7 cut(s) 365, 451, 563, 1105, 1168, 1289, 1381
FriOI GRGCYC 2 cut(s) 1018, 1727
Fsp4HI GCNGC 5 cut(s) 357, 590, 820, 1670, 1673
FspBI CTAG 4 cut(s) 50, 1040, 1193, 1764
FspI TGCGCA 1 cut(s) 1122
GlaI GCGC 2 cut(s) 1122, 1160
GluI GCNGC 5 cut(s) 357, 590, 820, 1670, 1673
GsaI CCCAGC 1 cut(s) 1049
GsuI CTGGAG 1 cut(s) 1779
HaeIII GGCC 6 cut(s) 737, 815, 1044, 1144, 1660, 1725
HapII CCGG 8 cut(s) 287, 857, 1090, 1315, 1346, 1404, 1548, 1664
HgaI GACGC 2 cut(s) 869, 1377
HhaI GCGC 2 cut(s) 1123, 1161
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 2 cut(s) 1121, 1159
HinP1I GCGC 2 cut(s) 1121, 1159
HincII GTYRAC 2 cut(s) 190, 1087
HindII GTYRAC 2 cut(s) 190, 1087
HinfI GANTC 5 cut(s) 327, 455, 495, 1063, 1637
HpaII CCGG 8 cut(s) 287, 857, 1090, 1315, 1346, 1404, 1548, 1664
HphI GGTGA 5 cut(s) 20, 232, 1821, 1827, 1837
Hpy166II GTNNAC 7 cut(s) 190, 1087, 1114, 1201, 1338, 1702, 1829
Hpy188III TCNNGA 6 cut(s) 380, 722, 989, 1169, 1596, 1651
Hpy8I GTNNAC 7 cut(s) 190, 1087, 1114, 1201, 1338, 1702, 1829
Hpy99I CGWCG 5 cut(s) 191, 446, 527, 1292, 1522
HpyAV CCTTC 3 cut(s) 253, 1402, 1774
HpyCH4III ACNGT 5 cut(s) 154, 215, 1342, 1518, 1736
HpyCH4IV ACGT 5 cut(s) 71, 180, 1083, 1698, 1869
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 353, 1666
HpyF3I CTNAG 4 cut(s) 559, 729, 1477, 1774
HpySE526I ACGT 5 cut(s) 71, 180, 1083, 1698, 1869
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 2 cut(s) 1121, 1159
LmnI GCTCC 3 cut(s) 850, 1021, 1760
Lsp1109I GCAGC 4 cut(s) 368, 601, 831, 1659
LweI GCATC 3 cut(s) 343, 1146, 1375
MaeI CTAG 4 cut(s) 50, 1040, 1193, 1764
MaeII ACGT 5 cut(s) 71, 180, 1083, 1698, 1869
MaeIII GTNAC 8 cut(s) 8, 200, 221, 1147, 1677, 1707, 1753, 1843
MboII GAAGA 7 cut(s) 394, 570, 941, 1072, 1445, 1546, 1798
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 5 cut(s) 40, 126, 1018, 1340, 1727
MlsI TGGCCA 2 cut(s) 737, 815
MluCI AATT 5 cut(s) 635, 864, 1281, 1501, 1588
MluNI TGGCCA 2 cut(s) 737, 815
MlyI GAGTC 2 cut(s) 464, 1057
MmeI TCCRAC 4 cut(s) 106, 237, 1231, 1500
Mox20I TGGCCA 2 cut(s) 737, 815
MscI TGGCCA 2 cut(s) 737, 815
MseI TTAA 3 cut(s) 195, 741, 1817
MslI CAYNNNNRTG 6 cut(s) 26, 524, 615, 1154, 1261, 1714
Msp20I TGGCCA 2 cut(s) 737, 815
MspA1I CMGCKG 2 cut(s) 336, 1672
MspI CCGG 8 cut(s) 287, 857, 1090, 1315, 1346, 1404, 1548, 1664
MspR9I CCNGG 6 cut(s) 159, 288, 472, 912, 1405, 1740
MvaI CCWGG 4 cut(s) 159, 472, 912, 1740
MwoI GCNNNNNNNGC 3 cut(s) 102, 353, 1666
NciI CCSGG 2 cut(s) 288, 1405
NheI GCTAGC 1 cut(s) 1192
NlaIV GGNNCC 9 cut(s) 477, 699, 1017, 1070, 1098, 1409, 1668, 1724, 1725
NmuCI GTSAC 6 cut(s) 8, 200, 1147, 1707, 1753, 1843
NsbI TGCGCA 1 cut(s) 1122
NspI RCATGY 4 cut(s) 589, 651, 935, 1708
OliI CACNNNNGTG 1 cut(s) 1714
PaeI GCATGC 2 cut(s) 651, 935
PagI TCATGA 1 cut(s) 721
PceI AGGCCT 1 cut(s) 1144
PciI ACATGT 1 cut(s) 1704
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 3 cut(s) 327, 495, 1637
PflFI GACNNNGTC 2 cut(s) 446, 1066
PinAI ACCGGT 1 cut(s) 1345
PkrI GCNGC 5 cut(s) 358, 591, 821, 1671, 1674
PleI GAGTC 2 cut(s) 463, 1057
PpsI GAGTC 2 cut(s) 463, 1057
Ppu21I YACGTR 1 cut(s) 1699
PpuMI RGGWCCY 1 cut(s) 1097
PscI ACATGT 1 cut(s) 1704
PshBI ATTAAT 1 cut(s) 741
Psp1406I AACGTT 1 cut(s) 1869
Psp5II RGGWCCY 1 cut(s) 1097
Psp6I CCWGG 4 cut(s) 157, 470, 910, 1738
PspFI CCCAGC 1 cut(s) 1045
PspGI CCWGG 4 cut(s) 157, 470, 910, 1738
PspN4I GGNNCC 9 cut(s) 477, 699, 1017, 1070, 1098, 1409, 1668, 1724, 1725
PspOMI GGGCCC 1 cut(s) 1723
PspPI GGNCC 9 cut(s) 598, 1043, 1068, 1097, 1408, 1658, 1723, 1724, 1736
PspPPI RGGWCCY 1 cut(s) 1097
PstI CTGCAG 2 cut(s) 981, 1677
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 475
PsyI GACNNNGTC 2 cut(s) 446, 1066
RsaI GTAC 7 cut(s) 217, 983, 1344, 1570, 1608, 1697, 1865
RsaNI GTAC 7 cut(s) 216, 982, 1343, 1569, 1607, 1696, 1864
RseI CAYNNNNRTG 6 cut(s) 26, 524, 615, 1154, 1261, 1714
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 3 cut(s) 195, 741, 1817
SatI GCNGC 5 cut(s) 357, 590, 820, 1670, 1673
Sau96I GGNCC 9 cut(s) 598, 1043, 1068, 1097, 1408, 1658, 1723, 1724, 1736
ScaI AGTACT 2 cut(s) 983, 1608
SchI GAGTC 2 cut(s) 464, 1057
ScrFI CCNGG 6 cut(s) 159, 288, 472, 912, 1405, 1740
SduI GDGCHC 5 cut(s) 40, 126, 1018, 1340, 1727
SfaNI GCATC 3 cut(s) 343, 1146, 1375
SfcI CTRYAG 2 cut(s) 977, 1673
SinI GGWCC 5 cut(s) 598, 1068, 1097, 1408, 1736
SmiMI CAYNNNNRTG 6 cut(s) 26, 524, 615, 1154, 1261, 1714
SmlI CTYRAG 1 cut(s) 1169
SmoI CTYRAG 1 cut(s) 1169
SphI GCATGC 2 cut(s) 651, 935
Sse9I AATT 5 cut(s) 635, 864, 1281, 1501, 1588
SseBI AGGCCT 1 cut(s) 1144
SsiI CCGC 3 cut(s) 336, 1294, 1670
SspMI CTAG 4 cut(s) 50, 1040, 1193, 1764
StuI AGGCCT 1 cut(s) 1144
StyD4I CCNGG 6 cut(s) 157, 286, 470, 910, 1403, 1738
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 5 cut(s) 154, 215, 1342, 1518, 1736
TaiI ACGT 5 cut(s) 74, 183, 1086, 1701, 1872
TaqI TCGA 8 cut(s) 189, 278, 330, 952, 1574, 1595, 1624, 1640
TaqII GACCGA 1 cut(s) 1056
TasI AATT 5 cut(s) 635, 864, 1281, 1501, 1588
TatI WGTACW 4 cut(s) 981, 1568, 1606, 1863
TauI GCSGC 1 cut(s) 1672
TfiI GAWTC 3 cut(s) 327, 495, 1637
Tru1I TTAA 3 cut(s) 195, 741, 1817
Tru9I TTAA 3 cut(s) 195, 741, 1817
TscAI CASTG 4 cut(s) 718, 1345, 1564, 1762
TseFI GTSAC 6 cut(s) 8, 200, 1147, 1707, 1753, 1843
TseI GCWGC 4 cut(s) 356, 589, 819, 1672
Tsp45I GTSAC 6 cut(s) 8, 200, 1147, 1707, 1753, 1843
TspDTI ATGAA 7 cut(s) 401, 487, 710, 868, 1219, 1293, 1384
TspGWI ACGGA 3 cut(s) 175, 440, 448
TspRI CASTG 4 cut(s) 718, 1345, 1564, 1762
Tth111I GACNNNGTC 2 cut(s) 446, 1066
VneI GTGCAC 1 cut(s) 1336
VpaK11BI GGWCC 5 cut(s) 598, 1068, 1097, 1408, 1736
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 1054
XceI RCATGY 4 cut(s) 589, 651, 935, 1708
XmaJI CCTAGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 4 cut(s) 50, 1040, 1193, 1764
ZraI GACGTC 1 cut(s) 72
ZrmI AGTACT 2 cut(s) 983, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.