Rroxscaffold_5G00383460

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014090
Physical Location & Seq
Forward (+)
63053214 .. 63058065
4852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383460.1

Sequence Viewer

Length: 1446 bp
ATGATGCTCACCACCAGTACGAGTCTCGATCACACCATCGTCGAGAGTGTTATAGCCAGTTTAGGCAAAGGCTTCGATTTAACCTCGGATTTCCGGCTTAAGTATTGCAAAGGCAAACAACGGTACCCTCTTGTTCTTGCCGATGAAGTTCGCGATGCTGTTCCGGCCACTTGGGACCCACTTGCCATTTCAAGATTCATAGAAAAATATGGGACGCACATCGTGGTGGGTTTGAGTGTTGGGGGTCAGGATGTGGTGTTGGTTAAGCAAGATAAATCTTCAAATTTGGGGCCATCAGACCTCAAAACTCACTTAAACGAGCTTGGAGATCAGCATTTTACGGGGACTTGTAATTTCTCCCCACTCCTCTCCAAAGCAAAAGACCATAAACGCAAGGCGCCACAGGCATTCAATGTCTTTGATGCTCCGCAGTCTGTTGCCCTCAACGGCTTCTCCACCGCAAACACAAAAGATTACAGCTTCATTCCTATCACTTCTCTCCTTAAAGGTGTTCCCGGCAAAGGTTTCTTGTCCCATGCCATCAACCTCTATCTCAGATACAAGCCTCCTATAGCTGATTTGCCCTACTTTCTTGAATTTCAAGTATCACACAAAGTTTGGGCTCCCCTTCACAATGACCTACGTTTAGGTCCTGCCACAAATAGCAAATCTAATGCATCATCACCTGCTATTCACTTCAGCTTGATGGGTCCTAGGTTATATGTAAACACCACACAGGTTAGAGTTGGAAATAATGCCCCTGTCACAGGGATGCGCTTGTTTCTTGAGGGCATGAAATGCAACAGGCTAGCCATCCACCTCCAACATCTAGCAAACACACCATTGATGTTGGAGAACAAGATCGATGACACAACAATGTGGCGAGGATCAGAAGAGATCGACGATGACCGGTACTTTGAAGCAACAAACGGGAAAAAGTTCTCCCACGTGTGCACGGCTCCAGTAACGTACGACCCAAAATGGATATCTTCGGGCATAACCTCGGCCTTCATTGTCTCAGGAGCACAACTTGTGGTCAAGCAACACGGCTCAAAGAATGTTCTCCATCTTCGGCTCTTGTTCTCCAAAGTCTTGGACTCTTTCTTAGTGCAATCAAATTGGGCAGAGTCATGTTCTTCAACTAAATCGTCGTCGTCTGGCCTTTTGAATGCGATGAGTAGACCAATTTCTGGGAACTATTGGGGAAAAGAGAAAGTAGAACCTGCCGCGGAGGATGTGATATTGGATTCGAGTGTTTTTCCTGCAGGGCCTCCGGTGCCTTTGCCTAGGAAAAAATTTGTGAAGCATGTAAACACCTCACATGTATGCAAAGGGCCGCAGGATAGTCCTGGCCATTGGTTGGTCACCGGAGCAAAGCTCAACTTGGTCAAGGGAAAGATTGGTTTGCAAGTCAAGTTCTCTCTGTTAAACCAAGCAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

481

Amino Acids

52.84

Weight (kDa)

9.34

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 40 - 111 3.8e-09 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 2 cut(s) 694, 1231
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 3 cut(s) 123, 397, 1276
AccB7I CCANNNNNTGG 2 cut(s) 1190, 1360
AccI GTMKAC 1 cut(s) 1180
AccII CGCG 2 cut(s) 153, 1229
AciI CCGC 5 cut(s) 428, 459, 1227, 1229, 1337
AclWI GGATC 1 cut(s) 895
AcoI YGGCCR 2 cut(s) 165, 1351
AcsI RAATTY 3 cut(s) 283, 596, 1295
AcuI CTGAAG 1 cut(s) 682
AcvI CACGTG 1 cut(s) 949
AcyI GRCGYC 1 cut(s) 398
AdeI CACNNNGTG 1 cut(s) 223
AfaI GTAC 4 cut(s) 19, 125, 914, 971
AfiI CCNNNNNNNGG 4 cut(s) 521, 767, 1190, 1360
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 2 cut(s) 948, 1321
AgeI ACCGGT 1 cut(s) 909
AgsI TTSAA 8 cut(s) 192, 282, 412, 596, 602, 920, 1140, 1168
AjnI CCWGG 1 cut(s) 1348
AluBI AGCT 5 cut(s) 322, 480, 575, 702, 1378
AluI AGCT 5 cut(s) 322, 480, 575, 702, 1378
Alw21I GWGCWC 2 cut(s) 956, 1027
Alw26I GTCTC 2 cut(s) 29, 1021
Alw44I GTGCAC 1 cut(s) 952
AlwI GGATC 1 cut(s) 895
AoxI GGCC 7 cut(s) 165, 290, 1005, 1159, 1268, 1334, 1351
ApaLI GTGCAC 1 cut(s) 952
ApoI RAATTY 3 cut(s) 283, 596, 1295
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AsiGI ACCGGT 1 cut(s) 909
Asp700I GAANNNNTTC 1 cut(s) 938
Asp718I GGTACC 1 cut(s) 123
AspA2I CCTAGG 2 cut(s) 713, 1286
AspLEI GCGC 2 cut(s) 400, 777
AspS9I GGNCC 6 cut(s) 175, 290, 650, 710, 1268, 1334
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 675, 1357
AsuNHI GCTAGC 1 cut(s) 808
AvaII GGWCC 3 cut(s) 175, 650, 710
AvrII CCTAGG 2 cut(s) 713, 1286
BaeGI GKGCMC 1 cut(s) 956
BaeI ACNNNNGTAYC 2 cut(s) 107, 140
BalI TGGCCA 1 cut(s) 1353
BanI GGYRCC 3 cut(s) 123, 397, 1276
BanII GRGCYC 1 cut(s) 625
BbrPI CACGTG 1 cut(s) 949
Bbv12I GWGCWC 2 cut(s) 956, 1027
BccI CCATC 6 cut(s) 44, 301, 548, 700, 821, 1074
BceAI ACGGC 3 cut(s) 463, 972, 1063
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 1 cut(s) 1350
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 2 cut(s) 29, 1021
BfaI CTAG 4 cut(s) 714, 809, 830, 1287
BfmI CTRYAG 2 cut(s) 570, 1263
BfoI RGCGCY 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 98
BfuAI ACCTGC 2 cut(s) 694, 1231
BisI GCNGC 2 cut(s) 1227, 1337
BlnI CCTAGG 2 cut(s) 713, 1286
BlsI GCNGC 2 cut(s) 1228, 1338
Bme1390I CCNGG 2 cut(s) 516, 1350
Bme18I GGWCC 3 cut(s) 175, 650, 710
BmgT120I GGNCC 6 cut(s) 175, 290, 650, 710, 1268, 1334
BmiI GGNNCC 9 cut(s) 125, 176, 177, 291, 399, 624, 711, 960, 1278
BmrFI CCNGG 2 cut(s) 516, 1350
BmsI GCATC 4 cut(s) 145, 412, 686, 762
BmtI GCTAGC 1 cut(s) 812
BplI GAGNNNNNCTC 2 cut(s) 1362, 1394
BpmI CTGGAG 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 1 cut(s) 516
Bsa29I ATCGAT 1 cut(s) 864
BsaAI YACGTR 1 cut(s) 949
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 5 cut(s) 84, 713, 1002, 1227, 1286
BsaWI WCCGGW 3 cut(s) 909, 1273, 1367
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 767, 1190, 1360
Bse118I RCCGGY 1 cut(s) 909
Bse1I ACTGG 3 cut(s) 15, 57, 962
BseBI CCWGG 1 cut(s) 1350
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 5 cut(s) 84, 713, 1002, 1227, 1286
BseGI GGATG 4 cut(s) 256, 777, 813, 1240
BseLI CCNNNNNNNGG 4 cut(s) 521, 767, 1190, 1360
BseMII CTCAG 2 cut(s) 568, 1032
BseNI ACTGG 3 cut(s) 15, 57, 962
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 956
Bsh1236I CGCG 2 cut(s) 153, 1229
BshFI GGCC 7 cut(s) 167, 292, 1007, 1161, 1270, 1336, 1353
BshNI GGYRCC 3 cut(s) 123, 397, 1276
BshTI ACCGGT 1 cut(s) 909
BshVI ATCGAT 1 cut(s) 864
BsiHKAI GWGCWC 2 cut(s) 956, 1027
BsiSI CCGG 6 cut(s) 94, 164, 516, 910, 1274, 1368
BsiWI CGTACG 1 cut(s) 969
BslFI GGGAC 4 cut(s) 188, 226, 358, 517
BslI CCNNNNNNNGG 4 cut(s) 521, 767, 1190, 1360
BsmAI GTCTC 2 cut(s) 29, 1021
BsmFI GGGAC 4 cut(s) 188, 226, 358, 517
BsmI GAATGC 2 cut(s) 407, 1174
BsnI GGCC 7 cut(s) 167, 292, 1007, 1161, 1270, 1336, 1353
Bsp1286I GDGCHC 3 cut(s) 625, 956, 1027
Bsp143I GATC 5 cut(s) 28, 328, 861, 887, 897
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 5 cut(s) 428, 459, 1227, 1229, 1337
BspANI GGCC 7 cut(s) 167, 292, 1007, 1161, 1270, 1336, 1353
BspCNI CTCAG 2 cut(s) 567, 1031
BspDI ATCGAT 1 cut(s) 864
BspFNI CGCG 2 cut(s) 153, 1229
BspLI GGNNCC 9 cut(s) 125, 176, 177, 291, 399, 624, 711, 960, 1278
BspMAI CTGCAG 1 cut(s) 1267
BspMI ACCTGC 2 cut(s) 694, 1231
BspOI GCTAGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 895
BspT107I GGYRCC 3 cut(s) 123, 397, 1276
BspTI CTTAAG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 909
BsrI ACTGG 3 cut(s) 15, 57, 962
BssAI RCCGGY 1 cut(s) 909
BssECI CCNNGG 5 cut(s) 84, 713, 1002, 1227, 1286
BssMI GATC 5 cut(s) 28, 328, 861, 887, 897
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 2 cut(s) 713, 1286
Bst2UI CCWGG 1 cut(s) 1350
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 888
BstACI GRCGYC 1 cut(s) 398
BstAFI CTTAAG 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 798
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 1 cut(s) 810
BstDEI CTNAG 3 cut(s) 554, 1018, 1105
BstDSI CCRYGG 1 cut(s) 1227
BstEII GGTNACC 1 cut(s) 1363
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 4 cut(s) 256, 777, 813, 1240
BstFNI CGCG 2 cut(s) 153, 1229
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 777
BstKTI GATC 5 cut(s) 31, 331, 864, 890, 900
BstMAI GTCTC 2 cut(s) 29, 1021
BstMBI GATC 5 cut(s) 28, 328, 861, 887, 897
BstMWI GCNNNNNNNGC 4 cut(s) 164, 404, 798, 1276
BstNI CCWGG 1 cut(s) 1350
BstNSI RCATGY 2 cut(s) 1310, 1325
BstPI GGTNACC 1 cut(s) 1363
BstSCI CCNGG 2 cut(s) 514, 1348
BstSFI CTRYAG 2 cut(s) 570, 1263
BstSLI GKGCMC 1 cut(s) 956
BstUI CGCG 2 cut(s) 153, 1229
BstXI CCANNNNNNTGG 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 864
BsuRI GGCC 7 cut(s) 167, 292, 1007, 1161, 1270, 1336, 1353
BsuTUI ATCGAT 1 cut(s) 864
BtgI CCRYGG 1 cut(s) 1227
BtgZI GCGATG 2 cut(s) 168, 1187
BtsCI GGATG 4 cut(s) 256, 777, 813, 1240
BtuMI TCGCGA 1 cut(s) 153
BveI ACCTGC 2 cut(s) 694, 1231
Cac8I GCNNGC 1 cut(s) 810
CfoI GCGC 2 cut(s) 400, 777
Cfr10I RCCGGY 1 cut(s) 909
Cfr13I GGNCC 6 cut(s) 175, 290, 650, 710, 1268, 1334
Cfr42I CCGCGG 1 cut(s) 1230
ClaI ATCGAT 1 cut(s) 864
CseI GACGC 1 cut(s) 223
Csp6I GTAC 4 cut(s) 18, 124, 913, 970
CspAI ACCGGT 1 cut(s) 909
CviAII CATG 5 cut(s) 536, 793, 1131, 1307, 1322
CviQI GTAC 4 cut(s) 18, 124, 913, 970
DdeI CTNAG 3 cut(s) 554, 1018, 1105
DinI GGCGCC 1 cut(s) 399
DpnI GATC 5 cut(s) 30, 330, 863, 889, 899
DpnII GATC 5 cut(s) 28, 328, 861, 887, 897
DraIII CACNNNGTG 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 165, 1351
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Eco130I CCWWGG 2 cut(s) 713, 1286
Eco24I GRGCYC 1 cut(s) 625
Eco32I GATATC 1 cut(s) 987
Eco47I GGWCC 3 cut(s) 175, 650, 710
Eco57I CTGAAG 1 cut(s) 682
Eco72I CACGTG 1 cut(s) 949
Eco91I GGTNACC 1 cut(s) 1363
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoO109I RGGNCCY 4 cut(s) 175, 650, 710, 1268
EcoO65I GGTNACC 1 cut(s) 1363
EcoRII CCWGG 1 cut(s) 1348
EcoRV GATATC 1 cut(s) 987
EcoT14I CCWWGG 2 cut(s) 713, 1286
EcoT22I ATGCAT 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 625
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 713, 1286
FaeI CATG 5 cut(s) 539, 796, 1134, 1310, 1325
FalI AAGNNNNNCTT 2 cut(s) 1367, 1399
FaqI GGGAC 4 cut(s) 188, 226, 358, 517
FatI CATG 5 cut(s) 535, 792, 1130, 1306, 1321
FblI GTMKAC 1 cut(s) 1180
Fnu4HI GCNGC 2 cut(s) 1227, 1337
FokI GGATG 4 cut(s) 263, 784, 800, 1247
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 2 cut(s) 1227, 1337
FspBI CTAG 4 cut(s) 714, 809, 830, 1287
GlaI GCGC 2 cut(s) 399, 776
GluI GCNGC 2 cut(s) 1227, 1337
GsuI CTGGAG 1 cut(s) 945
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 7 cut(s) 167, 292, 1007, 1161, 1270, 1336, 1353
HapII CCGG 6 cut(s) 94, 164, 516, 910, 1274, 1368
HgaI GACGC 1 cut(s) 223
HhaI GCGC 2 cut(s) 400, 777
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 5 cut(s) 539, 796, 1134, 1310, 1325
Hin6I GCGC 2 cut(s) 398, 775
HinP1I GCGC 2 cut(s) 398, 775
HinfI GANTC 5 cut(s) 22, 195, 1097, 1127, 1247
HpaII CCGG 6 cut(s) 94, 164, 516, 910, 1274, 1368
HphI GGTGA 2 cut(s) 675, 1357
Hpy166II GTNNAC 4 cut(s) 727, 954, 1181, 1312
Hpy188I TCNGA 4 cut(s) 88, 298, 557, 892
Hpy188III TCNNGA 8 cut(s) 26, 43, 152, 192, 248, 593, 785, 1020
Hpy8I GTNNAC 4 cut(s) 727, 954, 1181, 1312
Hpy99I CGWCG 4 cut(s) 44, 905, 1153, 1156
HpyAV CCTTC 2 cut(s) 638, 1018
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 3 cut(s) 643, 948, 968
HpyCH4V TGCA 8 cut(s) 108, 677, 801, 954, 1111, 1265, 1329, 1408
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 404, 798, 1276
HpyF3I CTNAG 3 cut(s) 554, 1018, 1105
HpySE526I ACGT 3 cut(s) 643, 948, 968
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 5 cut(s) 539, 796, 1134, 1310, 1325
HspAI GCGC 2 cut(s) 398, 775
KasI GGCGCC 1 cut(s) 397
KflI GGGWCCC 1 cut(s) 175
KpnI GGTACC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 1230
Kzo9I GATC 5 cut(s) 28, 328, 861, 887, 897
LmnI GCTCC 5 cut(s) 430, 628, 964, 1022, 1370
LweI GCATC 4 cut(s) 145, 412, 686, 762
MaeI CTAG 4 cut(s) 714, 809, 830, 1287
MaeII ACGT 3 cut(s) 643, 948, 968
MaeIII GTNAC 3 cut(s) 763, 964, 1363
MalI GATC 5 cut(s) 30, 330, 863, 889, 899
MboI GATC 5 cut(s) 28, 328, 861, 887, 897
MboII GAAGA 5 cut(s) 270, 905, 981, 1061, 1128
MhlI GDGCHC 3 cut(s) 625, 956, 1027
MlsI TGGCCA 1 cut(s) 1353
MluCI AATT 6 cut(s) 283, 352, 596, 1117, 1185, 1295
MluNI TGGCCA 1 cut(s) 1353
Mly113I GGCGCC 1 cut(s) 398
MlyI GAGTC 3 cut(s) 31, 1091, 1136
MmeI TCCRAC 3 cut(s) 727, 831, 847
Mox20I TGGCCA 1 cut(s) 1353
Mph1103I ATGCAT 1 cut(s) 679
MroXI GAANNNNTTC 1 cut(s) 938
MscI TGGCCA 1 cut(s) 1353
MseI TTAA 6 cut(s) 80, 99, 264, 314, 504, 1427
MslI CAYNNNNRTG 4 cut(s) 224, 770, 875, 1324
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 1 cut(s) 1229
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 6 cut(s) 94, 164, 516, 910, 1274, 1368
MspR9I CCNGG 2 cut(s) 516, 1350
Mva1269I GAATGC 2 cut(s) 407, 1174
MvaI CCWGG 1 cut(s) 1350
MvnI CGCG 2 cut(s) 153, 1229
MwoI GCNNNNNNNGC 4 cut(s) 164, 404, 798, 1276
NarI GGCGCC 1 cut(s) 398
NciI CCSGG 1 cut(s) 516
NdeII GATC 5 cut(s) 28, 328, 861, 887, 897
NheI GCTAGC 1 cut(s) 808
NlaIII CATG 5 cut(s) 539, 796, 1134, 1310, 1325
NlaIV GGNNCC 9 cut(s) 125, 176, 177, 291, 399, 624, 711, 960, 1278
NmeAIII GCCGAG 1 cut(s) 983
NmuCI GTSAC 2 cut(s) 763, 1363
NruI TCGCGA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 2 cut(s) 1310, 1325
PaqCI CACCTGC 1 cut(s) 694
PciI ACATGT 1 cut(s) 1321
PctI GAATGC 2 cut(s) 407, 1174
PdmI GAANNNNTTC 1 cut(s) 938
PfeI GAWTC 2 cut(s) 195, 1247
Pfl23II CGTACG 1 cut(s) 969
PflMI CCANNNNNTGG 2 cut(s) 1190, 1360
PinAI ACCGGT 1 cut(s) 909
PkrI GCNGC 2 cut(s) 1228, 1338
PleI GAGTC 3 cut(s) 30, 1091, 1135
PluTI GGCGCC 1 cut(s) 401
PmaCI CACGTG 1 cut(s) 949
PmlI CACGTG 1 cut(s) 949
PpsI GAGTC 3 cut(s) 30, 1091, 1135
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 3 cut(s) 175, 650, 710
PscI ACATGT 1 cut(s) 1321
Psp5II RGGWCCY 3 cut(s) 175, 650, 710
Psp6I CCWGG 1 cut(s) 1348
PspCI CACGTG 1 cut(s) 949
PspEI GGTNACC 1 cut(s) 1363
PspGI CCWGG 1 cut(s) 1348
PspLI CGTACG 1 cut(s) 969
PspN4I GGNNCC 9 cut(s) 125, 176, 177, 291, 399, 624, 711, 960, 1278
PspPI GGNCC 6 cut(s) 175, 290, 650, 710, 1268, 1334
PspPPI RGGWCCY 3 cut(s) 175, 650, 710
PstI CTGCAG 1 cut(s) 1267
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 4 cut(s) 19, 125, 914, 971
RsaNI GTAC 4 cut(s) 18, 124, 913, 970
RseI CAYNNNNRTG 4 cut(s) 224, 770, 875, 1324
SacII CCGCGG 1 cut(s) 1230
SaqAI TTAA 6 cut(s) 80, 99, 264, 314, 504, 1427
SatI GCNGC 2 cut(s) 1227, 1337
Sau3AI GATC 5 cut(s) 28, 328, 861, 887, 897
Sau96I GGNCC 6 cut(s) 175, 290, 650, 710, 1268, 1334
SbfI CCTGCAGG 1 cut(s) 1267
SchI GAGTC 3 cut(s) 31, 1091, 1136
ScrFI CCNGG 2 cut(s) 516, 1350
SdaI CCTGCAGG 1 cut(s) 1267
SduI GDGCHC 3 cut(s) 625, 956, 1027
SfaNI GCATC 4 cut(s) 145, 412, 686, 762
SfcI CTRYAG 2 cut(s) 570, 1263
SfoI GGCGCC 1 cut(s) 399
Sfr303I CCGCGG 1 cut(s) 1230
SgrBI CCGCGG 1 cut(s) 1230
SinI GGWCC 3 cut(s) 175, 650, 710
SmiMI CAYNNNNRTG 4 cut(s) 224, 770, 875, 1324
SmlI CTYRAG 2 cut(s) 98, 785
SmoI CTYRAG 2 cut(s) 98, 785
Sse8387I CCTGCAGG 1 cut(s) 1267
Sse9I AATT 6 cut(s) 283, 352, 596, 1117, 1185, 1295
SsiI CCGC 5 cut(s) 428, 459, 1227, 1229, 1337
SspDI GGCGCC 1 cut(s) 397
SspMI CTAG 4 cut(s) 714, 809, 830, 1287
StyD4I CCNGG 2 cut(s) 514, 1348
StyI CCWWGG 2 cut(s) 713, 1286
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 3 cut(s) 646, 951, 971
TaqI TCGA 6 cut(s) 27, 42, 75, 864, 900, 1250
TasI AATT 6 cut(s) 283, 352, 596, 1117, 1185, 1295
TauI GCSGC 2 cut(s) 1229, 1339
TfiI GAWTC 2 cut(s) 195, 1247
Tru1I TTAA 6 cut(s) 80, 99, 264, 314, 504, 1427
Tru9I TTAA 6 cut(s) 80, 99, 264, 314, 504, 1427
TseFI GTSAC 2 cut(s) 763, 1363
Tsp45I GTSAC 2 cut(s) 763, 1363
TspDTI ATGAA 5 cut(s) 159, 187, 472, 809, 1000
Van91I CCANNNNNTGG 2 cut(s) 1190, 1360
Vha464I CTTAAG 1 cut(s) 98
VneI GTGCAC 1 cut(s) 952
VpaK11BI GGWCC 3 cut(s) 175, 650, 710
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 3 cut(s) 283, 596, 1295
XceI RCATGY 2 cut(s) 1310, 1325
XmaJI CCTAGG 2 cut(s) 713, 1286
XmiI GTMKAC 1 cut(s) 1180
XmnI GAANNNNTTC 1 cut(s) 938
XspI CTAG 4 cut(s) 714, 809, 830, 1287
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.