Rh4CG422800

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014090
Physical Location & Seq
Forward (+)
66619166 .. 66623597
4432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG422800.1

Sequence Viewer

Length: 1344 bp
ATGATGAGCTTTGGAGACATTCTCCGATCGGAAAGAGAGAAAGAGATGATGCTCACCACCAGTACTAGTCTCGATCTCACCATCGTCGAGAGTGTTATAGCCAGTTTAGGCAAAGGCTTCGATTTAACCTCGGATTTCCGGCTTCAGTATTGCAAAGGCAAACAACGGTTGGTTGCCCTAAACGACGGCGACAAAAGAGAGCTCGTCGTGCCTGGTTTTGGTTCCATTCCGGACGTCTCTTGCGACATTAAATGCGACAAGGGCGACCGGACGAGGTGCCAGACGGACATGTTCAGCTTCAGTCAGATGTCGGAGTATATCAACCAGAAGTGTAGTGTTCCCGGGAAAGTGCCGTCGGGGTTGTTCAATTCGATGTTCGGGTTCGATAGTGGTTCGTGGGGGAGCGATGCGGCCAATATAAAATGCTTGGGTGTGGATGGATATTTCATCATCTTCTTCAGTGTTCATATTGATAGGTACCCTCTTGTTCTTGCCGATGAAGTTCGCGATGCTGTTCCGGCCACTTGGGACCCACTTGCCATTTCAAGATTCATAGAAAAATATGGAACGCACATCGTGGTGGGTTTGAGTGTTGGGGGTCAGGATGTGGTGTTGGTTAAGCAAGATAAATCATCAAATTTGGGGCCATCAGACCTCAAAACTCACTTAAACGAGCTTGGAGATCAGCATTTTACGGGGACTTGTAATTTCTCCCCACTCCTCTCCAAAGCAAAAGACCATAAACACAAGGCGCCACAGGCATTCAATGTCTTTGATGCTCCGCAGTCTGTTGCCCTCAACGGCTTCTCCACCGCAAACACAAAAGATGGAATTACTGTGATAAGCTCTAAAAAGGGAGGTGATCCAACGGCCAGTAGCCACAGCGAGTGGCTTCTAACAGTTCCGTCAATGCCGGACGCTATTCAGTACAGCTCCATTCCTATCACTTCTCTCCTTAAAGGTGTTCCCGGCAAAGGTTTCTTGTCCCATGCCATCAACCTCTATCTCAGATACAAGCCTCCTATAGCTGATTTGCCCTACTTTCTTGAATTTCAAGTATCACACAAAGTTTGGGCTCCCCTTCACAATGACCTACGTTTAGGTCCTGCCACAAATAGCAAATCTAATCCATCATCACCTGCTATTCACTTCAGCTTGATGGGTCCTAGGTTATATGTAAACACCACACAGGTTAGAGTTGGAAATAATGCCCCTGTCACAGGGATGCGCTTGTTTCTTGAGGGCATGAAATGCAACAGGTATATATTTAATCAACATTTATTCAATCTCTTCTTAACAATTTCATTGGTGGATCCAAAAATGTGGTGCAATAGGGCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

447

Amino Acids

49.32

Weight (kDa)

8.26

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 121 - 335 2.4e-28 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1147
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 1147
Acc65I GGTACC 1 cut(s) 477
AccB1I GGYRCC 3 cut(s) 276, 477, 751
AccII CGCG 1 cut(s) 507
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 3 cut(s) 410, 782, 813
AclWI GGATC 3 cut(s) 857, 1307, 1320
AcoI YGGCCR 3 cut(s) 411, 519, 870
AcsI RAATTY 2 cut(s) 637, 1049
AcuI CTGAAG 4 cut(s) 128, 283, 442, 1135
AcyI GRCGYC 2 cut(s) 234, 752
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 3 cut(s) 64, 479, 929
AfiI CCNNNNNNNGG 3 cut(s) 218, 974, 1220
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 6 cut(s) 367, 546, 766, 1049, 1055, 1285
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 8 cut(s) 9, 202, 297, 676, 846, 933, 1028, 1155
AluI AGCT 8 cut(s) 9, 202, 297, 676, 846, 933, 1028, 1155
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 3 cut(s) 9, 74, 241
AlwI GGATC 3 cut(s) 857, 1307, 1320
Ama87I CYCGRG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 4 cut(s) 411, 519, 644, 870
ApoI RAATTY 2 cut(s) 637, 1049
Asp718I GGTACC 1 cut(s) 477
AspA2I CCTAGG 1 cut(s) 1166
AspLEI GCGC 2 cut(s) 754, 1230
AspS9I GGNCC 4 cut(s) 529, 644, 1103, 1163
AsuC2I CCSGG 3 cut(s) 342, 343, 969
AsuHPI GGTGA 4 cut(s) 46, 70, 872, 1128
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 3 cut(s) 529, 1103, 1163
AvrII CCTAGG 1 cut(s) 1166
BamHI GGATCC 1 cut(s) 1312
BanI GGYRCC 3 cut(s) 276, 477, 751
BanII GRGCYC 2 cut(s) 204, 1078
Bbv12I GWGCWC 1 cut(s) 204
BccI CCATC 7 cut(s) 89, 431, 655, 821, 1001, 1138, 1153
BceAI ACGGC 4 cut(s) 202, 337, 817, 885
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 213
BcnI CCSGG 3 cut(s) 342, 343, 969
BcoDI GTCTC 3 cut(s) 9, 74, 241
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 3 cut(s) 66, 1167, 1342
BfmI CTRYAG 1 cut(s) 1023
BfoI RGCGCY 1 cut(s) 755
BfuAI ACCTGC 1 cut(s) 1147
BisI GCNGC 1 cut(s) 411
BlnI CCTAGG 1 cut(s) 1166
BlsI GCNGC 1 cut(s) 412
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 4 cut(s) 213, 342, 343, 969
Bme18I GGWCC 3 cut(s) 529, 1103, 1163
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 4 cut(s) 529, 644, 1103, 1163
BmrFI CCNGG 4 cut(s) 213, 342, 343, 969
BmsI GCATC 5 cut(s) 39, 397, 499, 766, 1215
BplI GAGNNNNNCTC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 1259
BpuMI CCSGG 3 cut(s) 342, 343, 969
BsaHI GRCGYC 2 cut(s) 234, 752
BsaJI CCNNGG 3 cut(s) 129, 341, 1166
BsaWI WCCGGW 2 cut(s) 229, 267
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 974, 1220
Bse1I ACTGG 3 cut(s) 60, 102, 873
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 129, 341, 1166
BseGI GGATG 3 cut(s) 442, 610, 1230
BseLI CCNNNNNNNGG 3 cut(s) 218, 974, 1220
BseMII CTCAG 1 cut(s) 1021
BseNI ACTGG 3 cut(s) 60, 102, 873
BseRI GAGGAG 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 2 cut(s) 29, 268
BshFI GGCC 4 cut(s) 413, 521, 646, 872
BshNI GGYRCC 3 cut(s) 276, 477, 751
BsiEI CGRYCG 2 cut(s) 29, 268
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 7 cut(s) 139, 230, 268, 342, 518, 914, 969
BslFI GGGAC 3 cut(s) 542, 712, 970
BslI CCNNNNNNNGG 3 cut(s) 218, 974, 1220
BsmAI GTCTC 3 cut(s) 9, 74, 241
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 3 cut(s) 542, 712, 970
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 4 cut(s) 413, 521, 646, 872
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 204, 1078
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 5 cut(s) 26, 73, 682, 862, 1312
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 3 cut(s) 410, 782, 813
BspANI GGCC 4 cut(s) 413, 521, 646, 872
BspCNI CTCAG 1 cut(s) 1020
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 507
BspMI ACCTGC 1 cut(s) 1147
BspPI GGATC 3 cut(s) 857, 1307, 1320
BspT107I GGYRCC 3 cut(s) 276, 477, 751
BsrI ACTGG 3 cut(s) 60, 102, 873
BssECI CCNNGG 3 cut(s) 129, 341, 1166
BssMI GATC 5 cut(s) 26, 73, 682, 862, 1312
BssNI GRCGYC 2 cut(s) 234, 752
BssT1I CCWWGG 1 cut(s) 1166
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 168, 838, 901
Bst6I CTCTTC 1 cut(s) 1295
BstACI GRCGYC 2 cut(s) 234, 752
BstAPI GCANNNNNTGC 1 cut(s) 1251
BstDEI CTNAG 1 cut(s) 1007
BstENI CCTNNNNNAGG 1 cut(s) 1218
BstF5I GGATG 3 cut(s) 442, 610, 1230
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 755
BstHHI GCGC 2 cut(s) 754, 1230
BstKTI GATC 5 cut(s) 29, 76, 685, 865, 1315
BstMAI GTCTC 3 cut(s) 9, 74, 241
BstMBI GATC 5 cut(s) 26, 73, 682, 862, 1312
BstMCI CGRYCG 2 cut(s) 29, 268
BstMWI GCNNNNNNNGC 5 cut(s) 208, 261, 518, 758, 1251
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 4 cut(s) 211, 340, 341, 967
BstSFI CTRYAG 1 cut(s) 1023
BstUI CGCG 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 1312
BstXI CCANNNNNNTGG 1 cut(s) 1323
BstYI RGATCY 1 cut(s) 1312
BsuRI GGCC 4 cut(s) 413, 521, 646, 872
BtgZI GCGATG 2 cut(s) 420, 522
BtsCI GGATG 3 cut(s) 442, 610, 1230
BtsIMutI CAGTG 1 cut(s) 466
BtuMI TCGCGA 1 cut(s) 507
BveI ACCTGC 1 cut(s) 1147
CfoI GCGC 2 cut(s) 754, 1230
Cfr13I GGNCC 4 cut(s) 529, 644, 1103, 1163
Cfr9I CCCGGG 1 cut(s) 341
CseI GACGC 1 cut(s) 926
Csp6I GTAC 3 cut(s) 63, 478, 928
CviAII CATG 3 cut(s) 289, 989, 1246
CviQI GTAC 3 cut(s) 63, 478, 928
DdeI CTNAG 1 cut(s) 1007
DinI GGCGCC 1 cut(s) 753
DpnI GATC 5 cut(s) 28, 75, 684, 864, 1314
DpnII GATC 5 cut(s) 26, 73, 682, 862, 1312
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 411, 519, 870
Eam1104I CTCTTC 1 cut(s) 1295
EarI CTCTTC 1 cut(s) 1295
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 1166
Eco24I GRGCYC 2 cut(s) 204, 1078
Eco47I GGWCC 3 cut(s) 529, 1103, 1163
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 4 cut(s) 128, 283, 442, 1135
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 1218
EcoO109I RGGNCCY 3 cut(s) 529, 1103, 1163
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 1166
EcoT38I GRGCYC 2 cut(s) 204, 1078
EgeI GGCGCC 1 cut(s) 753
EheI GGCGCC 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 1166
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 3 cut(s) 292, 992, 1249
FaqI GGGAC 3 cut(s) 542, 712, 970
FatI CATG 3 cut(s) 288, 988, 1245
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 3 cut(s) 449, 617, 1237
FriOI GRGCYC 2 cut(s) 204, 1078
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 3 cut(s) 66, 1167, 1342
GlaI GCGC 2 cut(s) 753, 1229
GluI GCNGC 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 755
HaeIII GGCC 4 cut(s) 413, 521, 646, 872
HapII CCGG 7 cut(s) 139, 230, 268, 342, 518, 914, 969
HgaI GACGC 1 cut(s) 926
HhaI GCGC 2 cut(s) 754, 1230
Hin1I GRCGYC 2 cut(s) 234, 752
Hin1II CATG 3 cut(s) 292, 992, 1249
Hin6I GCGC 2 cut(s) 752, 1228
HinP1I GCGC 2 cut(s) 752, 1228
HinfI GANTC 1 cut(s) 549
HpaII CCGG 7 cut(s) 139, 230, 268, 342, 518, 914, 969
HphI GGTGA 4 cut(s) 46, 70, 872, 1128
Hpy166II GTNNAC 1 cut(s) 1180
Hpy188I TCNGA 7 cut(s) 26, 31, 133, 306, 313, 652, 1010
Hpy188III TCNNGA 8 cut(s) 71, 88, 230, 506, 546, 602, 1046, 1238
Hpy8I GTNNAC 1 cut(s) 1180
Hpy99I CGWCG 4 cut(s) 89, 188, 209, 358
HpyAV CCTTC 1 cut(s) 1091
HpyCH4III ACNGT 3 cut(s) 168, 838, 901
HpyCH4IV ACGT 2 cut(s) 234, 1096
HpyCH4V TGCA 3 cut(s) 153, 1254, 1329
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 261, 518, 758, 1251
HpyF3I CTNAG 1 cut(s) 1007
HpySE526I ACGT 2 cut(s) 234, 1096
Hsp92I GRCGYC 2 cut(s) 234, 752
Hsp92II CATG 3 cut(s) 292, 992, 1249
HspAI GCGC 2 cut(s) 752, 1228
KasI GGCGCC 1 cut(s) 751
KflI GGGWCCC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 481
Kzo9I GATC 5 cut(s) 26, 73, 682, 862, 1312
LmnI GCTCC 4 cut(s) 402, 784, 938, 1081
LweI GCATC 5 cut(s) 39, 397, 499, 766, 1215
MaeI CTAG 3 cut(s) 66, 1167, 1342
MaeII ACGT 2 cut(s) 234, 1096
MaeIII GTNAC 1 cut(s) 1216
MalI GATC 5 cut(s) 28, 75, 684, 864, 1314
MboI GATC 5 cut(s) 26, 73, 682, 862, 1312
MboII GAAGA 3 cut(s) 445, 448, 1282
MflI RGATCY 1 cut(s) 1312
MhlI GDGCHC 2 cut(s) 204, 1078
MluCI AATT 6 cut(s) 367, 637, 706, 831, 1049, 1299
Mly113I GGCGCC 1 cut(s) 752
MmeI TCCRAC 3 cut(s) 291, 890, 1180
MroI TCCGGA 1 cut(s) 229
MseI TTAA 7 cut(s) 125, 249, 618, 668, 957, 1269, 1295
MslI CAYNNNNRTG 2 cut(s) 578, 1223
MspI CCGG 7 cut(s) 139, 230, 268, 342, 518, 914, 969
MspR9I CCNGG 4 cut(s) 213, 342, 343, 969
Mva1269I GAATGC 1 cut(s) 761
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 5 cut(s) 208, 261, 518, 758, 1251
NarI GGCGCC 1 cut(s) 752
NciI CCSGG 3 cut(s) 342, 343, 969
NdeII GATC 5 cut(s) 26, 73, 682, 862, 1312
NlaIII CATG 3 cut(s) 292, 992, 1249
NmuCI GTSAC 1 cut(s) 1216
NruI TCGCGA 1 cut(s) 507
NspI RCATGY 1 cut(s) 292
PaqCI CACCTGC 1 cut(s) 1147
PciI ACATGT 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 761
PfeI GAWTC 1 cut(s) 549
PkrI GCNGC 1 cut(s) 412
Ple19I CGATCG 1 cut(s) 29
PluTI GGCGCC 1 cut(s) 755
PpuMI RGGWCCY 3 cut(s) 529, 1103, 1163
PscI ACATGT 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 3 cut(s) 529, 1103, 1163
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspPI GGNCC 4 cut(s) 529, 644, 1103, 1163
PspPPI RGGWCCY 3 cut(s) 529, 1103, 1163
PsuI RGATCY 1 cut(s) 1312
PvuI CGATCG 1 cut(s) 29
RruI TCGCGA 1 cut(s) 507
RsaI GTAC 3 cut(s) 64, 479, 929
RsaNI GTAC 3 cut(s) 63, 478, 928
RseI CAYNNNNRTG 2 cut(s) 578, 1223
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 7 cut(s) 125, 249, 618, 668, 957, 1269, 1295
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 5 cut(s) 26, 73, 682, 862, 1312
Sau96I GGNCC 4 cut(s) 529, 644, 1103, 1163
ScaI AGTACT 1 cut(s) 64
ScrFI CCNGG 4 cut(s) 213, 342, 343, 969
SduI GDGCHC 2 cut(s) 204, 1078
SfaNI GCATC 5 cut(s) 39, 397, 499, 766, 1215
SfcI CTRYAG 1 cut(s) 1023
SfoI GGCGCC 1 cut(s) 753
SinI GGWCC 3 cut(s) 529, 1103, 1163
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 578, 1223
SmlI CTYRAG 1 cut(s) 1238
SmoI CTYRAG 1 cut(s) 1238
SpeI ACTAGT 1 cut(s) 65
Sse9I AATT 6 cut(s) 367, 637, 706, 831, 1049, 1299
SsiI CCGC 3 cut(s) 410, 782, 813
SspDI GGCGCC 1 cut(s) 751
SspMI CTAG 3 cut(s) 66, 1167, 1342
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 211, 340, 341, 967
StyI CCWWGG 1 cut(s) 1166
TaaI ACNGT 3 cut(s) 168, 838, 901
TaiI ACGT 2 cut(s) 237, 1099
TaqI TCGA 5 cut(s) 72, 87, 120, 371, 384
TasI AATT 6 cut(s) 367, 637, 706, 831, 1049, 1299
TatI WGTACW 2 cut(s) 62, 927
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 1 cut(s) 549
Tru1I TTAA 7 cut(s) 125, 249, 618, 668, 957, 1269, 1295
Tru9I TTAA 7 cut(s) 125, 249, 618, 668, 957, 1269, 1295
TscAI CASTG 1 cut(s) 466
TseFI GTSAC 1 cut(s) 1216
Tsp45I GTSAC 1 cut(s) 1216
TspDTI ATGAA 6 cut(s) 436, 455, 513, 541, 1262, 1293
TspGWI ACGGA 2 cut(s) 299, 894
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 1 cut(s) 466
VpaK11BI GGWCC 3 cut(s) 529, 1103, 1163
XagI CCTNNNNNAGG 1 cut(s) 1218
XapI RAATTY 2 cut(s) 637, 1049
XceI RCATGY 1 cut(s) 292
XmaI CCCGGG 1 cut(s) 341
XmaJI CCTAGG 1 cut(s) 1166
XspI CTAG 3 cut(s) 66, 1167, 1342
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.