pycom16g01550

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014090
Physical Location & Seq
Forward (+)
958037 .. 959174
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01550.1

Sequence Viewer

Length: 645 bp
ATGGTCTTTCAAGCCCACAAACTTTGGGCTCCGGTTCTGAATGAGCTGGCTCTAGGCCCAGTCTCAAATAGGACAACTCAGTCCCCTGCAATCCACGTCTGCTTGATGAGTCCTCAGCTATATGTAAACACTGCACACGTTAGGGTTGCAAACAGGCCTGTCATAGGGATGCGCTTGTATCTTGAGGGCAGGAAATTTTCCCATGTGTGCACCGTGCTGGTAAAACACGACCCCAAACAGATGTCCTCCAAGAAAGATGCGTCCTTCGTAGTGTGCGGGGTCCAGCTTGTCGTAAAGAAACACGACACAAAGAATGTCGTCCATCTTCGGCTCTTGTTCTCCAAAATCTCAGATCATTTTGTTGTCCAATCAAATTGGACTGAACCACCTACATCGATTTCCGGGAGTAACTGGTCGAGAGAGAAAGTACTGCCCGATGATAATCAAATGATTGTGGACTCGAGTGTGTTTCCGACAGGGCCTCCGGGCCCACAAGGCGGTCCAGGGTATTGGTTTGTCACCGGAGCTAGGCTCAACTTGGTGAAGGGGAAGATAAGTTTGCAAGTCAAGTTCTCTTCGTTAAACTTCTGTTCACCGTCACCATCAGTCTCCCTCCGAGATTTGATGTACAAGTTGTGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

215

Amino Acids

23.85

Weight (kDa)

9.81

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
AciI CCGC 2 cut(s) 276, 498
AcsI RAATTY 1 cut(s) 194
AfaI GTAC 2 cut(s) 429, 629
AfiI CCNNNNNNNGG 3 cut(s) 164, 497, 528
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 1 cut(s) 11
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 4 cut(s) 46, 118, 286, 527
AluI AGCT 4 cut(s) 46, 118, 286, 527
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 2 cut(s) 67, 613
Alw44I GTGCAC 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 4 cut(s) 55, 155, 479, 487
ApaI GGGCCC 1 cut(s) 491
ApaLI GTGCAC 1 cut(s) 208
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 6 cut(s) 56, 280, 479, 487, 488, 500
AsuC2I CCSGG 2 cut(s) 403, 486
AsuHPI GGTGA 4 cut(s) 511, 553, 585, 591
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 2 cut(s) 280, 500
BaeGI GKGCMC 2 cut(s) 212, 491
BanII GRGCYC 2 cut(s) 31, 491
Bbv12I GWGCWC 1 cut(s) 212
BbvCI CCTCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 330, 610
BciT130I CCWGG 1 cut(s) 504
BcnI CCSGG 2 cut(s) 403, 486
BcoDI GTCTC 2 cut(s) 67, 613
BfaI CTAG 2 cut(s) 53, 528
BglI GCCNNNNNGGC 1 cut(s) 495
BmcAI AGTACT 1 cut(s) 429
Bme1390I CCNGG 3 cut(s) 403, 486, 504
Bme18I GGWCC 2 cut(s) 280, 500
BmeT110I CYCGRG 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 6 cut(s) 56, 280, 479, 487, 488, 500
BmiI GGNNCC 3 cut(s) 30, 281, 489
BmrFI CCNGG 3 cut(s) 403, 486, 504
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 2 cut(s) 159, 247
BmuI ACTGGG 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 516, 548
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 403, 486
Bsa29I ATCGAT 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 503
BsaWI WCCGGW 2 cut(s) 31, 521
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 497, 528
Bse1I ACTGG 2 cut(s) 59, 416
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 504
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 1 cut(s) 174
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 164, 497, 528
BseMII CTCAG 3 cut(s) 92, 128, 363
BseNI ACTGG 2 cut(s) 59, 416
BseSI GKGCMC 2 cut(s) 212, 491
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 4 cut(s) 57, 157, 481, 489
BshVI ATCGAT 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 4 cut(s) 32, 402, 485, 522
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 164, 497, 528
BsmAI GTCTC 2 cut(s) 67, 613
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 4 cut(s) 57, 157, 481, 489
BsoBI CYCGRG 1 cut(s) 460
Bsp120I GGGCCC 1 cut(s) 487
Bsp1286I GDGCHC 3 cut(s) 31, 212, 491
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 2 cut(s) 276, 498
BspANI GGCC 4 cut(s) 57, 157, 481, 489
BspCNI CTCAG 3 cut(s) 91, 127, 362
BspDI ATCGAT 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 30, 281, 489
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 2 cut(s) 59, 416
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 214, 597
Bst6I CTCTTC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 3 cut(s) 78, 114, 349
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 2 cut(s) 67, 613
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 495
BstNI CCWGG 1 cut(s) 504
BstSCI CCNGG 3 cut(s) 401, 484, 502
BstSLI GKGCMC 2 cut(s) 212, 491
BstXI CCANNNNNNTGG 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 395
BsuRI GGCC 4 cut(s) 57, 157, 481, 489
BsuTUI ATCGAT 1 cut(s) 395
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 48
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 6 cut(s) 56, 280, 479, 487, 488, 500
ClaI ATCGAT 1 cut(s) 395
CseI GACGC 1 cut(s) 249
Csp6I GTAC 2 cut(s) 428, 628
CspCI CAANNNNNGTGG 2 cut(s) 5, 40
CviAII CATG 1 cut(s) 203
CviQI GTAC 2 cut(s) 428, 628
DdeI CTNAG 3 cut(s) 78, 114, 349
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Eco147I AGGCCT 1 cut(s) 157
Eco24I GRGCYC 2 cut(s) 31, 491
Eco47I GGWCC 2 cut(s) 280, 500
Eco88I CYCGRG 1 cut(s) 460
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 502
EcoT38I GRGCYC 2 cut(s) 31, 491
FaeI CATG 1 cut(s) 206
FaiI YATR 4 cut(s) 121, 123, 164, 204
FaqI GGGAC 1 cut(s) 67
FatI CATG 1 cut(s) 202
FauI CCCGC 1 cut(s) 269
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 2 cut(s) 31, 491
FspBI CTAG 2 cut(s) 53, 528
GlaI GCGC 1 cut(s) 173
HaeIII GGCC 4 cut(s) 57, 157, 481, 489
HapII CCGG 4 cut(s) 32, 402, 485, 522
HgaI GACGC 1 cut(s) 249
HhaI GCGC 1 cut(s) 174
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 2 cut(s) 109, 458
HpaII CCGG 4 cut(s) 32, 402, 485, 522
HphI GGTGA 4 cut(s) 511, 553, 585, 591
Hpy166II GTNNAC 4 cut(s) 127, 210, 457, 593
Hpy188I TCNGA 4 cut(s) 39, 352, 474, 617
Hpy188III TCNNGA 2 cut(s) 182, 417
Hpy8I GTNNAC 4 cut(s) 127, 210, 457, 593
HpyAV CCTTC 2 cut(s) 274, 538
HpyCH4III ACNGT 2 cut(s) 214, 597
HpyCH4IV ACGT 2 cut(s) 96, 138
HpyCH4V TGCA 5 cut(s) 89, 134, 149, 210, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 495
HpyF3I CTNAG 3 cut(s) 78, 114, 349
HpySE526I ACGT 2 cut(s) 96, 138
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 2 cut(s) 34, 524
LweI GCATC 2 cut(s) 159, 247
MaeI CTAG 2 cut(s) 53, 528
MaeII ACGT 2 cut(s) 96, 138
MaeIII GTNAC 3 cut(s) 407, 517, 597
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 3 cut(s) 317, 562, 567
MhlI GDGCHC 3 cut(s) 31, 212, 491
MluCI AATT 2 cut(s) 194, 373
MlyI GAGTC 2 cut(s) 118, 452
MmeI TCCRAC 1 cut(s) 497
MnlI CCTC 5 cut(s) 123, 178, 256, 492, 623
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 4 cut(s) 32, 402, 485, 522
MspR9I CCNGG 3 cut(s) 403, 486, 504
MvaI CCWGG 1 cut(s) 504
MwoI GCNNNNNNNGC 1 cut(s) 495
NciI CCSGG 2 cut(s) 403, 486
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 30, 281, 489
NmuCI GTSAC 2 cut(s) 517, 597
PaeR7I CTCGAG 1 cut(s) 460
PceI AGGCCT 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 401
PleI GAGTC 2 cut(s) 117, 452
PpsI GAGTC 2 cut(s) 117, 452
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 3 cut(s) 30, 281, 489
PspOMI GGGCCC 1 cut(s) 487
PspPI GGNCC 6 cut(s) 56, 280, 479, 487, 488, 500
PspXI VCTCGAGB 1 cut(s) 460
RsaI GTAC 2 cut(s) 429, 629
RsaNI GTAC 2 cut(s) 428, 628
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 1 cut(s) 581
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 6 cut(s) 56, 280, 479, 487, 488, 500
ScaI AGTACT 1 cut(s) 429
SchI GAGTC 2 cut(s) 118, 452
ScrFI CCNGG 3 cut(s) 403, 486, 504
SduI GDGCHC 3 cut(s) 31, 212, 491
SetI ASST 7 cut(s) 48, 99, 120, 141, 288, 391, 529
SfaNI GCATC 2 cut(s) 159, 247
Sfr274I CTCGAG 1 cut(s) 460
SinI GGWCC 2 cut(s) 280, 500
SlaI CTCGAG 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 182, 460
SmoI CTYRAG 2 cut(s) 182, 460
Sse9I AATT 2 cut(s) 194, 373
SseBI AGGCCT 1 cut(s) 157
SsiI CCGC 2 cut(s) 276, 498
SspMI CTAG 2 cut(s) 53, 528
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 3 cut(s) 401, 484, 502
TaaI ACNGT 2 cut(s) 214, 597
TaiI ACGT 2 cut(s) 99, 141
TaqI TCGA 3 cut(s) 395, 416, 461
TasI AATT 2 cut(s) 194, 373
TatI WGTACW 2 cut(s) 427, 627
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 517, 597
Tsp45I GTSAC 2 cut(s) 517, 597
TspRI CASTG 1 cut(s) 136
VneI GTGCAC 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 280, 500
XagI CCTNNNNNAGG 1 cut(s) 162
XapI RAATTY 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 460
XspI CTAG 2 cut(s) 53, 528
ZrmI AGTACT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.