FvH4_4g33680

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013391
Physical Location & Seq
Forward (+)
31824479 .. 31826359
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33680.t1

Sequence Viewer

Length: 534 bp
ATGACTTGTTTAGTTGCTAGAACGGGAAGGGAGTTGCAGAGGTACAACATGGGCCGCCGTCAAGTCGTAGGGTGCATACCTTATAGATACAAAAATGGCGTTGATGGTACCGAAAGCAAGGAGTTGGAATTTCTTGTGATAACTTCACAGAAAGGCAATGCATTCATGTTTCCTAAGGGTGGTTGGGAACTTGATGAATCTGTAGAAGAAGCTGCTTCAAGAGAATCGCTAGAAGAAGCAGGAGTTCTTGGAAATGTTGAGTGTCAATTGGGACAATGGAGTTTCATGAGCAAAAGACACGACACATATTATGAAGGCTACATGTTTCCTTTGTTTGTCAAGGAGCAACTGGATCTCTGGCCAGAGAAGAACGTACGACAACGCGTTTGGATGACTGCTGCCAAAGCTAGAGAAGCTTGTCAACATTGGTGGATGAAGGAAGCCTTGGACATTTTGGTCGAGCGGCTTACATCAGAACAGAATCAGAGGGATGAAGAAGATGCGAGTCTAGTATCGGCTTGTTCTCTGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

178

Amino Acids

20.49

Weight (kDa)

5.12

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 44 - 148 7.5e-09 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14860 AT2G01670
fragaria_vesca FvH4_4g33680
malus_domestica MD13G1029200.v1.1 MD16G1019500.v1.1
prunus_persica Prupe.1G332500_v2.0.a1
pyrus_communis pycom13g01780 pycom16g01750
rosa_chinensis RchiOBHm_Chr4g0443161
rosa_laevigata RLG00000005951
rosa_multiflora Rmu_sc0007968.1_g000007
rosa_roxburghii Rroxscaffold_5G00383760
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4AG399100 Rh4BG410700 Rh4CG426200 Rh4DG405800
rosa_wichuraiana Rw4G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
Acc65I GGTACC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 463
AccII CGCG 1 cut(s) 384
AciI CCGC 2 cut(s) 55, 463
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 359
AcsI RAATTY 1 cut(s) 128
AfaI GTAC 3 cut(s) 44, 109, 375
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 2 cut(s) 321, 382
AgsI TTSAA 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 4 cut(s) 212, 407, 416, 531
AluI AGCT 4 cut(s) 212, 407, 416, 531
AlwI GGATC 1 cut(s) 360
AoxI GGCC 2 cut(s) 52, 359
ApeKI GCWGC 2 cut(s) 212, 398
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp718I GGTACC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 52
AxyI CCTNAGG 1 cut(s) 174
BalI TGGCCA 1 cut(s) 361
BanI GGYRCC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 199, 385
BccI CCATC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 42
BfaI CTAG 4 cut(s) 18, 230, 408, 509
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 4 cut(s) 55, 213, 399, 464
BlsI GCNGC 4 cut(s) 56, 214, 400, 465
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 109
BmsI GCATC 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 354
Bse21I CCTNAGG 1 cut(s) 174
Bse3DI GCAATG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 3 cut(s) 396, 438, 496
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 199, 385
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 54, 361
BshNI GGYRCC 1 cut(s) 107
BsiWI CGTACG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 2 cut(s) 54, 361
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 2 cut(s) 55, 463
BspANI GGCC 2 cut(s) 54, 361
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 354
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 444
BstDEI CTNAG 2 cut(s) 174, 527
BstF5I GGATG 3 cut(s) 396, 438, 496
BstFNI CGCG 1 cut(s) 384
BstKTI GATC 1 cut(s) 355
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 2 cut(s) 404, 413
BstNSI RCATGY 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 199, 385
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 2 cut(s) 54, 361
BtsCI GGATG 3 cut(s) 396, 438, 496
CciI TCATGA 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 3 cut(s) 43, 108, 374
CspCI CAANNNNNGTGG 2 cut(s) 410, 445
CviAII CATG 4 cut(s) 49, 166, 286, 322
CviQI GTAC 3 cut(s) 43, 108, 374
DdeI CTNAG 2 cut(s) 174, 527
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 4 cut(s) 52, 169, 289, 325
FaiI YATR 8 cut(s) 50, 77, 84, 167, 287, 307, 312, 323
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 1 cut(s) 285
FatI CATG 4 cut(s) 48, 165, 285, 321
Fnu4HI GCNGC 4 cut(s) 55, 213, 399, 464
FokI GGATG 3 cut(s) 403, 445, 503
Fsp4HI GCNGC 4 cut(s) 55, 213, 399, 464
FspBI CTAG 4 cut(s) 18, 230, 408, 509
GluI GCNGC 4 cut(s) 55, 213, 399, 464
HaeIII GGCC 2 cut(s) 54, 361
Hin1II CATG 4 cut(s) 52, 169, 289, 325
HincII GTYRAC 1 cut(s) 422
HindII GTYRAC 1 cut(s) 422
HindIII AAGCTT 1 cut(s) 414
HinfI GANTC 4 cut(s) 197, 224, 481, 505
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 3 cut(s) 475, 486, 528
Hpy188III TCNNGA 2 cut(s) 219, 286
Hpy8I GTNNAC 1 cut(s) 422
HpyAV CCTTC 3 cut(s) 21, 308, 430
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 3 cut(s) 37, 75, 161
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 413
HpyF3I CTNAG 2 cut(s) 174, 527
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 4 cut(s) 52, 169, 289, 325
KpnI GGTACC 1 cut(s) 111
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 4 cut(s) 225, 335, 343, 375
Lsp1109I GCAGC 2 cut(s) 199, 385
LweI GCATC 1 cut(s) 490
MaeI CTAG 4 cut(s) 18, 230, 408, 509
MaeII ACGT 1 cut(s) 372
MalI GATC 1 cut(s) 354
MbiI CCGCTC 1 cut(s) 463
MboI GATC 1 cut(s) 352
MboII GAAGA 5 cut(s) 218, 245, 379, 506, 509
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 361
MluCI AATT 2 cut(s) 128, 266
MluI ACGCGT 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 361
MlyI GAGTC 1 cut(s) 514
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 2 cut(s) 33, 480
Mox20I TGGCCA 1 cut(s) 361
Mph1103I ATGCAT 1 cut(s) 163
MscI TGGCCA 1 cut(s) 361
Msp20I TGGCCA 1 cut(s) 361
MunI CAATTG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 161
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 404, 413
NdeII GATC 1 cut(s) 352
NlaIII CATG 4 cut(s) 52, 169, 289, 325
NlaIV GGNNCC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 325
PagI TCATGA 1 cut(s) 285
PciI ACATGT 1 cut(s) 321
PctI GAATGC 1 cut(s) 161
PfeI GAWTC 3 cut(s) 197, 224, 481
Pfl23II CGTACG 1 cut(s) 373
PkrI GCNGC 4 cut(s) 56, 214, 400, 465
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
PscI ACATGT 1 cut(s) 321
PspLI CGTACG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 3 cut(s) 44, 109, 375
RsaNI GTAC 3 cut(s) 43, 108, 374
SatI GCNGC 4 cut(s) 55, 213, 399, 464
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 514
SetI ASST 7 cut(s) 44, 82, 214, 375, 409, 418, 533
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 1 cut(s) 201
Sse9I AATT 2 cut(s) 128, 266
SsiI CCGC 2 cut(s) 55, 463
SspMI CTAG 4 cut(s) 18, 230, 408, 509
StyI CCWWGG 1 cut(s) 444
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 459
TasI AATT 2 cut(s) 128, 266
TauI GCSGC 2 cut(s) 57, 466
TfiI GAWTC 3 cut(s) 197, 224, 481
TseI GCWGC 2 cut(s) 212, 398
TspDTI ATGAA 6 cut(s) 154, 210, 274, 327, 449, 507
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 325
XspI CTAG 4 cut(s) 18, 230, 408, 509
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.