MD13G1029200.v1.1

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013391
Physical Location & Seq
Forward (+)
2103734 .. 2105004
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1029200.v1.1.491

Sequence Viewer

Length: 510 bp
ATGATGACTTGTTTAGTTTCTCGGACAGGAAGGGAATTGCAGAGGTACAACATGGGCCGCCGGCAAGTCGTAGGATGCATACCTTATAGATACAAAGATGGCAGTGATGAATTGGAAGTTCTTGTGATCACTTCACAGAAAGGCAATGCATTTATGTTCCCTAAGGGGGGTTGGGAACTTGATGAATCCGTAGAAGAAGCTGCTTCAAGAGAGTCACTAGAAGAAGCAGGTGTTTTAGGCAATGTTGAGTGTCAATTGGGAAAATGGTGTTTCATGAGCAAAAGCCAAGAGACATATTATGAAGGGTACATGTTTCCCTTGTTTGTCAAGGAGCAACTAGACCTTTGGCCTGAGAAGAATGTACGACAAAGAATATGGATGACTGCAGCGAAAGCGAGAGAGGCATGCCAACATTGGTGGATGAAGGAAGCGTTGGACATTTTGGTCGAACGGCTTACCTCGGAACAAAAACAAGGGGAGGAGAATGTACTGGCTTGTTCTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

170

Amino Acids

19.66

Weight (kDa)

5.12

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 20 - 143 1.4e-09 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14860 AT2G01670
fragaria_vesca FvH4_4g33680
malus_domestica MD13G1029200.v1.1 MD16G1019500.v1.1
prunus_persica Prupe.1G332500_v2.0.a1
pyrus_communis pycom13g01780 pycom16g01750
rosa_chinensis RchiOBHm_Chr4g0443161
rosa_laevigata RLG00000005951
rosa_multiflora Rmu_sc0007968.1_g000007
rosa_roxburghii Rroxscaffold_5G00383760
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4AG399100 Rh4BG410700 Rh4CG426200 Rh4DG405800
rosa_wichuraiana Rw4G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 218
AasI GACNNNNNNGTC 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 218
AciI CCGC 1 cut(s) 58
AfaI GTAC 4 cut(s) 47, 308, 363, 489
AfiI CCNNNNNNNGG 2 cut(s) 166, 167
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 2 cut(s) 207, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 284
AoxI GGCC 2 cut(s) 55, 347
ApeKI GCWGC 2 cut(s) 200, 386
AspS9I GGNCC 1 cut(s) 55
AxyI CCTNAGG 1 cut(s) 162
BbvI GCAGC 2 cut(s) 187, 398
BccI CCATC 1 cut(s) 92
BceAI ACGGC 1 cut(s) 467
BclI TGATCA 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 2 cut(s) 218, 338
BfmI CTRYAG 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 218
BisI GCNGC 3 cut(s) 58, 201, 387
BlsI GCNGC 3 cut(s) 59, 202, 388
BmgT120I GGNCC 1 cut(s) 55
BmsI GCATC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 459
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 167
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 495
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 2 cut(s) 151, 247
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 3 cut(s) 80, 384, 426
BseLI CCNNNNNNNGG 2 cut(s) 166, 167
BseMI GCAATG 2 cut(s) 151, 247
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 495
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 187, 398
BshFI GGCC 2 cut(s) 57, 349
BsiSI CCGG 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 166, 167
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 2 cut(s) 57, 349
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 1 cut(s) 58
BspANI GGCC 2 cut(s) 57, 349
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 388
BspMI ACCTGC 1 cut(s) 218
BsrDI GCAATG 2 cut(s) 151, 247
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 1 cut(s) 495
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 62, 406
BstDEI CTNAG 2 cut(s) 162, 351
BstF5I GGATG 3 cut(s) 80, 384, 426
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 392, 401
BstNSI RCATGY 2 cut(s) 313, 408
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 187, 398
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 2 cut(s) 57, 349
BtsCI GGATG 3 cut(s) 80, 384, 426
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 109
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 2 cut(s) 62, 406
CciI TCATGA 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 55
Csp6I GTAC 4 cut(s) 46, 307, 362, 488
CspCI CAANNNNNGTGG 2 cut(s) 398, 433
CviAII CATG 4 cut(s) 52, 274, 310, 405
CviJI RGCY 6 cut(s) 57, 200, 285, 349, 454, 494
CviKI_1 RGCY 6 cut(s) 57, 200, 285, 349, 454, 494
CviQI GTAC 4 cut(s) 46, 307, 362, 488
DdeI CTNAG 2 cut(s) 162, 351
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
Eco81I CCTNAGG 1 cut(s) 162
EcoT22I ATGCAT 2 cut(s) 80, 151
FaeI CATG 4 cut(s) 55, 277, 313, 408
FatI CATG 4 cut(s) 51, 273, 309, 404
FbaI TGATCA 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 58, 201, 387
FokI GGATG 3 cut(s) 87, 391, 433
Fsp4HI GCNGC 3 cut(s) 58, 201, 387
FspBI CTAG 2 cut(s) 218, 338
GluI GCNGC 3 cut(s) 58, 201, 387
HaeIII GGCC 2 cut(s) 57, 349
HapII CCGG 1 cut(s) 61
Hin1II CATG 4 cut(s) 55, 277, 313, 408
HinfI GANTC 2 cut(s) 185, 212
HpaII CCGG 1 cut(s) 61
Hpy188I TCNGA 2 cut(s) 24, 463
Hpy188III TCNNGA 2 cut(s) 207, 274
HpyAV CCTTC 3 cut(s) 24, 296, 418
HpyCH4V TGCA 4 cut(s) 40, 78, 149, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 401
HpyF3I CTNAG 2 cut(s) 162, 351
Hsp92II CATG 4 cut(s) 55, 277, 313, 408
KroI GCCGGC 1 cut(s) 60
KroNI GCCGGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 5 cut(s) 12, 74, 213, 363, 476
Lsp1109I GCAGC 2 cut(s) 187, 398
LweI GCATC 1 cut(s) 65
MaeI CTAG 2 cut(s) 218, 338
MaeIII GTNAC 1 cut(s) 213
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 3 cut(s) 206, 233, 367
MfeI CAATTG 1 cut(s) 254
MluCI AATT 4 cut(s) 35, 110, 254, 505
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 4 cut(s) 36, 394, 469, 472
Mph1103I ATGCAT 2 cut(s) 80, 151
MroNI GCCGGC 1 cut(s) 60
MseI TTAA 1 cut(s) 508
MspI CCGG 1 cut(s) 61
MunI CAATTG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 392, 401
NaeI GCCGGC 1 cut(s) 62
NdeII GATC 1 cut(s) 126
NgoMIV GCCGGC 1 cut(s) 60
NlaIII CATG 4 cut(s) 55, 277, 313, 408
NmuCI GTSAC 1 cut(s) 213
NsiI ATGCAT 2 cut(s) 80, 151
NspI RCATGY 2 cut(s) 313, 408
PaeI GCATGC 1 cut(s) 408
PagI TCATGA 1 cut(s) 273
PaqCI CACCTGC 1 cut(s) 218
PciI ACATGT 1 cut(s) 309
PdiI GCCGGC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 3 cut(s) 59, 202, 388
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PscI ACATGT 1 cut(s) 309
PspPI GGNCC 1 cut(s) 55
PstI CTGCAG 1 cut(s) 388
RsaI GTAC 4 cut(s) 47, 308, 363, 489
RsaNI GTAC 4 cut(s) 46, 307, 362, 488
SaqAI TTAA 1 cut(s) 508
SatI GCNGC 3 cut(s) 58, 201, 387
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 221
SetI ASST 6 cut(s) 47, 85, 202, 232, 345, 461
SfaNI GCATC 1 cut(s) 65
SfcI CTRYAG 1 cut(s) 384
SphI GCATGC 1 cut(s) 408
Sse9I AATT 4 cut(s) 35, 110, 254, 505
SsiI CCGC 1 cut(s) 58
SspMI CTAG 2 cut(s) 218, 338
TaqI TCGA 1 cut(s) 447
TasI AATT 4 cut(s) 35, 110, 254, 505
TatI WGTACW 1 cut(s) 487
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 508
Tru9I TTAA 1 cut(s) 508
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 2 cut(s) 200, 386
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 5 cut(s) 123, 198, 262, 315, 437
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 1 cut(s) 109
XceI RCATGY 2 cut(s) 313, 408
XspI CTAG 2 cut(s) 218, 338
Zsp2I ATGCAT 2 cut(s) 80, 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.