MD16G1019500.v1.1

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013391
Physical Location & Seq
Forward (+)
1420150 .. 1422146
1997 bp
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UTR
Exon/CDS
Intron
MD16G1019500.v1.1.491

Sequence Viewer

Length: 522 bp
ATGACTTGTTTAGTTGCTCGGACGGGAAGGGAATTGCAGAGGTACAACATGGGCCGCCGGCAAGTCGTAGGATGCATTCCCTATAGATACAAAGATGGCAGTGATGGTGCCATTAGCAACGAATTGGAAGTCCTTGTGATCACTTCACAGAAAGGCAATGCATTTATGTTCCCTAAGGGTGGTTGGGAACTTGACGAATCCGTAGAAGAAGCTGCTTCAAGAGAGTCACTAGAAGAAGCAGGGGTCCTAGGCAATGTTGAGTGCCAATTGGGCAAATGGAGTTTCATGAGCAAAAGCCAAGAGACATATTATGAAGGGTACATGTTTCCCTTGTTTGTCAAGGAGCAACTAGATCTTTGGCCTGAGAAGAATGTACGACAAAGAATATGGATGACTACAACGAAAGCAAGAGAGGCATGCCAACATTGGTGGATGAAGGAAGCGTTGGACATTTTGGTCGAACGGCTTACCTCGGAACAGAAACAAGAGGAGGAGAATGTACTGGCTTGTTCTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

174

Amino Acids

20.07

Weight (kDa)

5.03

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 40 - 147 8.4e-09 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14860 AT2G01670
fragaria_vesca FvH4_4g33680
malus_domestica MD13G1029200.v1.1 MD16G1019500.v1.1
prunus_persica Prupe.1G332500_v2.0.a1
pyrus_communis pycom13g01780 pycom16g01750
rosa_chinensis RchiOBHm_Chr4g0443161
rosa_laevigata RLG00000005951
rosa_multiflora Rmu_sc0007968.1_g000007
rosa_roxburghii Rroxscaffold_5G00383760
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4AG399100 Rh4BG410700 Rh4CG426200 Rh4DG405800
rosa_wichuraiana Rw4G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 107
AciI CCGC 1 cut(s) 55
AfaI GTAC 4 cut(s) 44, 320, 375, 501
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 2 cut(s) 219, 517
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 296
AoxI GGCC 2 cut(s) 52, 359
ApeKI GCWGC 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AspA2I CCTAGG 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 52, 244
AvaII GGWCC 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 247
AxyI CCTNAGG 1 cut(s) 174
BanI GGYRCC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 199
BccI CCATC 2 cut(s) 89, 98
BceAI ACGGC 1 cut(s) 479
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 3 cut(s) 230, 248, 350
BfmI CTRYAG 1 cut(s) 82
BglI GCCNNNNNGGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 352
BisI GCNGC 2 cut(s) 55, 213
BlnI CCTAGG 1 cut(s) 247
BlsI GCNGC 2 cut(s) 56, 214
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 52, 244
BmiI GGNNCC 2 cut(s) 109, 245
BmsI GCATC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 247, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 1 cut(s) 507
Bse21I CCTNAGG 1 cut(s) 174
Bse3DI GCAATG 2 cut(s) 163, 259
BseDI CCNNGG 2 cut(s) 247, 471
BseGI GGATG 3 cut(s) 77, 396, 438
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 2 cut(s) 163, 259
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 2 cut(s) 503, 506
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 2 cut(s) 54, 361
BshNI GGYRCC 1 cut(s) 107
BsiSI CCGG 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 2 cut(s) 54, 361
Bsp143I GATC 2 cut(s) 138, 352
BspACI CCGC 1 cut(s) 55
BspANI GGCC 2 cut(s) 54, 361
BspCNI CTCAG 1 cut(s) 355
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 109, 245
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 163, 259
BsrFI RCCGGY 1 cut(s) 57
BsrI ACTGG 1 cut(s) 507
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 247, 471
BssMI GATC 2 cut(s) 138, 352
BssT1I CCWWGG 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 59, 418
BstDEI CTNAG 2 cut(s) 174, 363
BstF5I GGATG 3 cut(s) 77, 396, 438
BstKTI GATC 2 cut(s) 141, 355
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 138, 352
BstMWI GCNNNNNNNGC 2 cut(s) 270, 413
BstNSI RCATGY 2 cut(s) 325, 420
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 2 cut(s) 54, 361
BtsCI GGATG 3 cut(s) 77, 396, 438
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 59, 418
CciI TCATGA 1 cut(s) 285
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 2 cut(s) 52, 244
Csp6I GTAC 4 cut(s) 43, 319, 374, 500
CspCI CAANNNNNGTGG 2 cut(s) 410, 445
CviAII CATG 4 cut(s) 49, 286, 322, 417
CviJI RGCY 6 cut(s) 54, 212, 297, 361, 466, 506
CviKI_1 RGCY 6 cut(s) 54, 212, 297, 361, 466, 506
CviQI GTAC 4 cut(s) 43, 319, 374, 500
DdeI CTNAG 2 cut(s) 174, 363
DpnI GATC 2 cut(s) 140, 354
DpnII GATC 2 cut(s) 138, 352
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 2 cut(s) 77, 163
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 4 cut(s) 52, 289, 325, 420
FaiI YATR 9 cut(s) 50, 84, 167, 287, 307, 312, 323, 388, 418
FatI CATG 4 cut(s) 48, 285, 321, 416
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 55, 213
FokI GGATG 3 cut(s) 84, 403, 445
Fsp4HI GCNGC 2 cut(s) 55, 213
FspBI CTAG 3 cut(s) 230, 248, 350
GluI GCNGC 2 cut(s) 55, 213
HaeIII GGCC 2 cut(s) 54, 361
HapII CCGG 1 cut(s) 58
Hin1II CATG 4 cut(s) 52, 289, 325, 420
HinfI GANTC 2 cut(s) 197, 224
HpaII CCGG 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 21, 475
Hpy188III TCNNGA 2 cut(s) 219, 286
HpyAV CCTTC 3 cut(s) 21, 308, 430
HpyCH4V TGCA 3 cut(s) 37, 75, 161
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 413
HpyF3I CTNAG 2 cut(s) 174, 363
Hsp92II CATG 4 cut(s) 52, 289, 325, 420
KroI GCCGGC 1 cut(s) 57
KroNI GCCGGC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 2 cut(s) 138, 352
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 4 cut(s) 71, 225, 375, 488
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 62
MaeI CTAG 3 cut(s) 230, 248, 350
MaeIII GTNAC 1 cut(s) 225
MalI GATC 2 cut(s) 140, 354
MboI GATC 2 cut(s) 138, 352
MboII GAAGA 3 cut(s) 218, 245, 379
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 1 cut(s) 352
MluCI AATT 4 cut(s) 32, 122, 266, 517
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 5 cut(s) 33, 406, 481, 481, 484
Mph1103I ATGCAT 2 cut(s) 77, 163
MroNI GCCGGC 1 cut(s) 57
MseI TTAA 1 cut(s) 520
MspI CCGG 1 cut(s) 58
MunI CAATTG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 270, 413
NaeI GCCGGC 1 cut(s) 59
NdeII GATC 2 cut(s) 138, 352
NgoMIV GCCGGC 1 cut(s) 57
NlaIII CATG 4 cut(s) 52, 289, 325, 420
NlaIV GGNNCC 2 cut(s) 109, 245
NmuCI GTSAC 1 cut(s) 225
NsiI ATGCAT 2 cut(s) 77, 163
NspI RCATGY 2 cut(s) 325, 420
PaeI GCATGC 1 cut(s) 420
PagI TCATGA 1 cut(s) 285
PciI ACATGT 1 cut(s) 321
PctI GAATGC 1 cut(s) 75
PdiI GCCGGC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 2 cut(s) 56, 214
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
PpuMI RGGWCCY 1 cut(s) 244
PscI ACATGT 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 109, 245
PspPI GGNCC 2 cut(s) 52, 244
PspPPI RGGWCCY 1 cut(s) 244
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 4 cut(s) 44, 320, 375, 501
RsaNI GTAC 4 cut(s) 43, 319, 374, 500
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 2 cut(s) 55, 213
Sau3AI GATC 2 cut(s) 138, 352
Sau96I GGNCC 2 cut(s) 52, 244
SchI GAGTC 1 cut(s) 233
SetI ASST 3 cut(s) 44, 214, 473
SfaNI GCATC 1 cut(s) 62
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 244
SphI GCATGC 1 cut(s) 420
Sse9I AATT 4 cut(s) 32, 122, 266, 517
SsiI CCGC 1 cut(s) 55
SspMI CTAG 3 cut(s) 230, 248, 350
StyI CCWWGG 1 cut(s) 247
TaqI TCGA 1 cut(s) 459
TasI AATT 4 cut(s) 32, 122, 266, 517
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 3 cut(s) 274, 327, 449
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 244
XceI RCATGY 2 cut(s) 325, 420
XmaJI CCTAGG 1 cut(s) 247
XspI CTAG 3 cut(s) 230, 248, 350
Zsp2I ATGCAT 2 cut(s) 77, 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.