FvH4_4g35000

DCC family protein At1g52590

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014917
Physical Location & Seq
Forward (+)
32485052 .. 32487032
1981 bp
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UTR
Exon/CDS
Intron
FvH4_4g35000.t1

Sequence Viewer

Length: 660 bp
ATGACGGTGGCGAGGTTGATCAGATTGCTCCCAAAACGAGCTCCAATTTGGTCTTCTTTTTCATCACCAAAGCGTCTATTTTCCTCCTCTGACGTCGTTTCGAGCACCGCCGCCGACCTCGCCGGCGTCGGCGACGACGACGATGAAGACGCGCCCCTCTACTCTACCCCATCTGCTGCATCCTCGGCGGTTCTGCAGCCCACTCTGCTTCAGCCACGTGTGGTGGTGTACGATGGTGTCTGCCATCTCTGCCATGGAGGGGTGAAGTGGGTGATCAAAGCAGACAAGCACAGGAAGATCAAGTTCTGCTGTCTTCAGTCTGAGACAGCTGAGCCTTATTTGAGACTGTGTGGGCTTGATCGGGAGGATGTTCTTCGTCGGTTCTTGTTTGTTGAAGGCCCTGGTTCGTATCACCAGGGCTCCACTGCTGCGCTGAAAGTATTGTCATACTTGCCTCTCCCTTACTCTGCTTTAAGCGCACTCTTGGTAATCCCAACTCCTCTCAGAGAGATTGTCTATGATCATGTTGCCAAACGGCGGTATGACTGGTTTGGCAAGTCTGAAGATTGCTTGGTTTTGAAGGAGAAAGAGTTGCTCGAGCGTTTCATTGATAGGGAAGAGTTGGTGAGTCGAGGTGGATCAGATTTCTCAGAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF393

Protein Analysis

220

Amino Acids

24.46

Weight (kDa)

6.52

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 75 - 186 3.7e-26 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24095 AT1G24095
fragaria_vesca FvH4_4g35000
malus_domestica MD13G1087000.v1.1 MD16G1087300.v1.1
pyrus_communis pycom13g07500 pycom16g07490
rosa_chinensis RchiOBHm_Chr4g0444521
rosa_laevigata RLG00000005827
rosa_multiflora Rmu_co8099458.1_g000001 Rmu_sc0019173.1_g000001
rosa_rugosa Rorug04G0353300.1
rosa_samantha Rh4AG413600 Rh4BG425100 Rh4CG439700 Rh4DG420600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
AccII CGCG 1 cut(s) 152
AciI CCGC 4 cut(s) 108, 111, 188, 538
AclWI GGATC 1 cut(s) 646
AcuI CTGAAG 3 cut(s) 194, 299, 582
AcvI CACGTG 1 cut(s) 218
AcyI GRCGYC 2 cut(s) 93, 126
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 2 cut(s) 259, 537
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 2 cut(s) 395, 580
AjnI CCWGG 2 cut(s) 400, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 2 cut(s) 41, 329
AluI AGCT 2 cut(s) 41, 329
Alw21I GWGCWC 2 cut(s) 43, 107
Alw26I GTCTC 2 cut(s) 317, 337
AlwI GGATC 1 cut(s) 646
Ama87I CYCGRG 1 cut(s) 596
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 3 cut(s) 176, 196, 428
AspLEI GCGC 3 cut(s) 154, 433, 479
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 5 cut(s) 57, 274, 283, 404, 637
AvaI CYCGRG 1 cut(s) 596
BanII GRGCYC 2 cut(s) 43, 422
BbrPI CACGTG 1 cut(s) 218
BbsI GAAGAC 3 cut(s) 45, 153, 305
Bbv12I GWGCWC 2 cut(s) 43, 107
BbvI GCAGC 3 cut(s) 163, 208, 415
BccI CCATC 3 cut(s) 178, 227, 252
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 2 cut(s) 402, 416
BclI TGATCA 3 cut(s) 18, 273, 520
BcoDI GTCTC 2 cut(s) 317, 337
BfmI CTRYAG 1 cut(s) 194
BisI GCNGC 4 cut(s) 111, 177, 197, 429
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 4 cut(s) 112, 178, 198, 430
Bme1390I CCNGG 2 cut(s) 402, 416
BmeT110I CYCGRG 1 cut(s) 596
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 2 cut(s) 402, 416
BmsI GCATC 1 cut(s) 188
BpiI GAAGAC 3 cut(s) 45, 153, 305
Bpu1102I GCTNAGC 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 218
BsaHI GRCGYC 2 cut(s) 93, 126
BsaJI CCNNGG 4 cut(s) 183, 253, 400, 415
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 537
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 551
BseBI CCWGG 2 cut(s) 402, 416
BseDI CCNNGG 4 cut(s) 183, 253, 400, 415
BseGI GGATG 2 cut(s) 179, 373
BseLI CCNNNNNNNGG 2 cut(s) 259, 537
BseMII CTCAG 3 cut(s) 312, 321, 517
BseNI ACTGG 1 cut(s) 551
BseRI GAGGAG 2 cut(s) 76, 489
BseXI GCAGC 3 cut(s) 163, 208, 415
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 43, 107
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 2 cut(s) 259, 537
BsmAI GTCTC 2 cut(s) 317, 337
BsnI GGCC 1 cut(s) 399
BsoBI CYCGRG 1 cut(s) 596
Bsp1286I GDGCHC 3 cut(s) 43, 107, 422
Bsp143I GATC 6 cut(s) 18, 273, 297, 358, 520, 638
Bsp1720I GCTNAGC 1 cut(s) 330
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 4 cut(s) 108, 111, 188, 538
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 3 cut(s) 313, 322, 516
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 421
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 1 cut(s) 646
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 183, 253, 400, 415
BssMI GATC 6 cut(s) 18, 273, 297, 358, 520, 638
BssNI GRCGYC 2 cut(s) 93, 126
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 402, 416
Bst4CI ACNGT 2 cut(s) 7, 348
Bst6I CTCTTC 1 cut(s) 612
BstACI GRCGYC 2 cut(s) 93, 126
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 4 cut(s) 321, 330, 503, 649
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 2 cut(s) 179, 373
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 3 cut(s) 154, 433, 479
BstKTI GATC 6 cut(s) 21, 276, 300, 361, 523, 641
BstMAI GTCTC 2 cut(s) 317, 337
BstMBI GATC 6 cut(s) 18, 273, 297, 358, 520, 638
BstMWI GCNNNNNNNGC 5 cut(s) 119, 185, 205, 249, 476
BstNI CCWGG 2 cut(s) 402, 416
BstSCI CCNGG 2 cut(s) 400, 414
BstSFI CTRYAG 1 cut(s) 194
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 3 cut(s) 163, 208, 415
BstV2I GAAGAC 3 cut(s) 45, 153, 305
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 2 cut(s) 179, 373
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 1 cut(s) 124
CfoI GCGC 3 cut(s) 154, 433, 479
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 398
CseI GACGC 3 cut(s) 62, 115, 158
Csp6I GTAC 1 cut(s) 229
CviAII CATG 2 cut(s) 254, 524
CviJI RGCY 8 cut(s) 41, 199, 214, 329, 334, 355, 399, 420
CviKI_1 RGCY 8 cut(s) 41, 199, 214, 329, 334, 355, 399, 420
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 4 cut(s) 321, 330, 503, 649
DpnI GATC 6 cut(s) 20, 275, 299, 360, 522, 640
DpnII GATC 6 cut(s) 18, 273, 297, 358, 520, 638
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 2 cut(s) 43, 422
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 3 cut(s) 194, 299, 582
Eco72I CACGTG 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 400, 414
EcoT14I CCWWGG 1 cut(s) 253
EcoT38I GRGCYC 2 cut(s) 43, 422
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 2 cut(s) 257, 527
FaiI YATR 5 cut(s) 255, 448, 519, 525, 543
FatI CATG 2 cut(s) 253, 523
FbaI TGATCA 3 cut(s) 18, 273, 520
Fnu4HI GCNGC 4 cut(s) 111, 177, 197, 429
FokI GGATG 2 cut(s) 166, 380
FriOI GRGCYC 2 cut(s) 43, 422
Fsp4HI GCNGC 4 cut(s) 111, 177, 197, 429
GlaI GCGC 3 cut(s) 153, 432, 478
GluI GCNGC 4 cut(s) 111, 177, 197, 429
HaeIII GGCC 1 cut(s) 399
HapII CCGG 1 cut(s) 123
HgaI GACGC 3 cut(s) 62, 115, 158
HhaI GCGC 3 cut(s) 154, 433, 479
Hin1I GRCGYC 2 cut(s) 93, 126
Hin1II CATG 2 cut(s) 257, 527
Hin6I GCGC 3 cut(s) 152, 431, 477
HinP1I GCGC 3 cut(s) 152, 431, 477
HinfI GANTC 1 cut(s) 628
HpaII CCGG 1 cut(s) 123
HphI GGTGA 5 cut(s) 57, 274, 283, 404, 637
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 7 cut(s) 23, 91, 322, 506, 562, 643, 652
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 6 cut(s) 98, 131, 137, 140, 143, 381
HpyAV CCTTC 2 cut(s) 389, 574
HpyCH4III ACNGT 2 cut(s) 7, 348
HpyCH4IV ACGT 2 cut(s) 93, 217
HpyCH4V TGCA 2 cut(s) 179, 196
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 185, 205, 249, 476
HpyF3I CTNAG 4 cut(s) 321, 330, 503, 649
HpySE526I ACGT 2 cut(s) 93, 217
Hsp92I GRCGYC 2 cut(s) 93, 126
Hsp92II CATG 2 cut(s) 257, 527
HspAI GCGC 3 cut(s) 152, 431, 477
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 3 cut(s) 18, 273, 520
Kzo9I GATC 6 cut(s) 18, 273, 297, 358, 520, 638
LmnI GCTCC 3 cut(s) 33, 46, 425
LpnPI CCDG 7 cut(s) 136, 277, 387, 401, 414, 428, 532
Lsp1109I GCAGC 3 cut(s) 163, 208, 415
LweI GCATC 1 cut(s) 188
MaeII ACGT 2 cut(s) 93, 217
MalI GATC 6 cut(s) 20, 275, 299, 360, 522, 640
MboI GATC 6 cut(s) 18, 273, 297, 358, 520, 638
MboII GAAGA 7 cut(s) 45, 158, 305, 307, 365, 575, 629
MhlI GDGCHC 3 cut(s) 43, 107, 422
MluCI AATT 1 cut(s) 45
MlyI GAGTC 1 cut(s) 637
MreI CGCCGGCG 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 2 cut(s) 473, 658
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 402, 416
MvaI CCWGG 2 cut(s) 402, 416
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 5 cut(s) 119, 185, 205, 249, 476
NaeI GCCGGC 1 cut(s) 124
NcoI CCATGG 1 cut(s) 253
NdeII GATC 6 cut(s) 18, 273, 297, 358, 520, 638
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 2 cut(s) 257, 527
NlaIV GGNNCC 1 cut(s) 421
NmeAIII GCCGAG 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 124
PkrI GCNGC 4 cut(s) 112, 178, 198, 430
PleI GAGTC 1 cut(s) 636
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
PpsI GAGTC 1 cut(s) 636
Ppu21I YACGTR 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 400, 414
PspCI CACGTG 1 cut(s) 218
PspGI CCWGG 2 cut(s) 400, 414
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 398
PspXI VCTCGAGB 1 cut(s) 596
PstI CTGCAG 1 cut(s) 198
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 473, 658
SatI GCNGC 4 cut(s) 111, 177, 197, 429
Sau3AI GATC 6 cut(s) 18, 273, 297, 358, 520, 638
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 1 cut(s) 637
ScrFI CCNGG 2 cut(s) 402, 416
SduI GDGCHC 3 cut(s) 43, 107, 422
SetI ASST 7 cut(s) 17, 43, 96, 120, 220, 331, 637
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 194
Sfr274I CTCGAG 1 cut(s) 596
SgrAI CRCCGGYG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 1 cut(s) 45
SsiI CCGC 4 cut(s) 108, 111, 188, 538
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 400, 414
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 7, 348
TaiI ACGT 2 cut(s) 96, 220
TaqI TCGA 3 cut(s) 101, 597, 631
TasI AATT 1 cut(s) 45
TauI GCSGC 1 cut(s) 113
Tru1I TTAA 2 cut(s) 473, 658
Tru9I TTAA 2 cut(s) 473, 658
TscAI CASTG 1 cut(s) 430
TseI GCWGC 3 cut(s) 176, 196, 428
TspDTI ATGAA 3 cut(s) 51, 159, 595
TspRI CASTG 1 cut(s) 430
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 596
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.