FvH4_4g35150

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015016
Physical Location & Seq
Reverse (-)
32564344 .. 32565639
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35150.t1

Sequence Viewer

Length: 1296 bp
ATGGGACTTGTTCCATGTCCTTTGCCCTGCCGCTTACACAGTACAGATTCCGAGTCCAACTCTAGTTACCACCTCCACTCCGATCCTTCATCCTCCTCCTCAATATCTTCCCAGCCAAGCCTTGCCTCTCTTCCTTCAACCTCCTCAATATCTTCCCAACTAAGCCTTGCATCTGTCCCGTCACTATCACAACATGTACTACAACAAACCCCAACAACCCAACTCAACTGCATAGCAATCCTGAAATCCGACTCCTCCTCTGTCTGTTCCCTATTCATCTCCGGAGAGTTCCTCTACACCGGCTCATCCGACGGCGAGATACGATCATGGAGCTGCAGAGACATCTCCTCAAACACTTCCAAGCTGCTAGCCACAACCAACTCCACAGTCAAGTCTCTAGTGATTGTGGGCGACAAAATCTTCAGCGCTCACCAAGACCACAAAATACGAGTTTGGAAATTCGATACAGCCAACCCAAATGACCACAAATCCAAATGCAAATGCATGGCGACCCTGCCGACACTGAAAGACCGTCTCCCACGACTGTTCTCCCCCAAAAGCTACGTCGAAGTCCGGCGGCACAAGAAGTTAACGTGGGTGCATCATGTGGACGCAATATCAGCTCTGGCTGTATCGAAGGACGAGTCTCTTGTCTACTCCGCTTCATGGGACCGCACGTTCAAAATCTGGCGAACCTCCGACTTCAAGTGCTTGGAGTCTGTGAGCAATGCGCATGAAGATGCCATAAACGCAATAGTGGTGAGCAATGACGGATTTGTATACACCGGCTCGGCGGATAAGAAGATCAAGGTGTGGAAGAAGCTCACCGGAGAGAAGTATCACACACTTGTTGAGACACTAGAGAAGCACAAGTCGGCGGTGAACGCCTTGGTTTTTAGCTCCGACGGGGGAGTGTTGTACTCCGGCGCGTGTGATCGGTCGGTTCTGGTCTGGGAAAGAGAGAGTGGTGGTGGTGGGATGGTTGTGATCGGGGCGCTTAGAGGTCATACTAAAGCAATATTGTGTTTGGCGGTGGTGGGGGATTTGGTGTGCAGCGGTTCGGCGGATTGTAGTGTTAGGATTTGGAGGAGAGGGGTTGGTGATGATCGGAGTTACTCGTGTTTGGGAGTGTTGGAAGGTCATAGGCGACCGGTGAAGTGTTTAACGGCGGCGGTGGGTTTTGACGGTGATGATAAAAGTGGTTGTGTTGATTCTTTTGTTGTTTATAGTGGTAGTTTGGACTGTGACACGAAGGTGTGGCAGATACAGGTCCCTTATTTGGTTAATTCGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

432

Amino Acids

46.79

Weight (kDa)

8.39

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 45 - 323 6.3e-13 SCAP Beta-propeller
Beta-prop_TEP1_2nd PF25047 92 - 171 3.4e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 94 - 242 2.4e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 95 - 319 9.3e-15 Prp19 WD40 domain
WDR55 PF24796 193 - 272 1.2e-08 WDR55
WD40_Gbeta PF25391 195 - 362 6.7e-11 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 198 - 283 5.9e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 201 - 273 4.5e-09 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 201 - 322 3.8e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 201 - 323 2.9e-21 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 202 - 323 7.3e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 202 - 363 7.5e-11 WDHD1 first WD40 domain
NBCH_WD40 PF20426 206 - 276 2.9e-06 Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd PF25172 206 - 321 4.7e-15 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 207 - 388 2e-13 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 208 - 361 4.2e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 243 - 423 1e-23 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 256 - 319 3e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 262 - 363 5.2e-07 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 280 - 362 3.9e-06 WDR55
Beta-prop_WDR3_1st PF25173 283 - 362 3.1e-11 WDR3 first beta-propeller domain
WD40 PF00400 283 - 318 4.9e-07 WD domain, G-beta repeat
EIF3I PF24805 287 - 367 1.3e-06 EIF3I
Beta-prop_THOC3 PF25174 290 - 363 8.7e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 303 - 389 6.6e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 332 - 424 1e-05 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24130
fragaria_vesca FvH4_4g35150
malus_domestica MD13G1085800.v1.1 MD16G1085100.v1.1
prunus_persica Prupe.1G260900_v2.0.a1
pyrus_communis pycom13g07420 pycom16g07360
rosa_chinensis RchiOBHm_Chr4g0444751
rosa_laevigata RLG00000005805
rosa_multiflora Rmu_sc0000096.1_g000011
rosa_roxburghii Rroxscaffold_5G00385270
rosa_samantha Rh4AG415800 Rh4BG427100 Rh4CG442000 Rh4DG423100
rosa_wichuraiana Rw4G035680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 732
AccI GTMKAC 2 cut(s) 654, 780
AccII CGCG 1 cut(s) 929
AccIII TCCGGA 1 cut(s) 281
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 406
AfaI GTAC 3 cut(s) 43, 198, 920
AfeI AGCGCT 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 666, 1279
AflIII ACRYGT 1 cut(s) 193
AgeI ACCGGT 1 cut(s) 1150
AgsI TTSAA 3 cut(s) 138, 682, 706
AleI CACNNNNGTG 1 cut(s) 1253
AloI GAACNNNNNNTCC 2 cut(s) 662, 694
AluBI AGCT 6 cut(s) 333, 364, 561, 623, 823, 900
AluI AGCT 6 cut(s) 333, 364, 561, 623, 823, 900
Alw26I GTCTC 5 cut(s) 333, 399, 539, 651, 848
AlwI GGATC 1 cut(s) 77
Aor13HI TCCGGA 1 cut(s) 281
Aor51HI AGCGCT 1 cut(s) 427
ApeKI GCWGC 3 cut(s) 333, 364, 1053
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 4 cut(s) 549, 581, 629, 661
AsiGI ACCGGT 1 cut(s) 1150
AspLEI GCGC 4 cut(s) 428, 733, 929, 997
AspS9I GGNCC 2 cut(s) 670, 1270
AsuHPI GGTGA 7 cut(s) 422, 772, 817, 892, 1112, 1165, 1199
AsuNHI GCTAGC 1 cut(s) 367
AvaII GGWCC 2 cut(s) 670, 1270
BauI CACGAG 1 cut(s) 1117
BbvI GCAGC 3 cut(s) 320, 351, 1065
BccI CCATC 1 cut(s) 973
BceAI ACGGC 2 cut(s) 328, 1182
BcoDI GTCTC 5 cut(s) 333, 399, 539, 651, 848
BfaI CTAG 4 cut(s) 63, 368, 398, 860
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 2 cut(s) 429, 998
BisI GCNGC 6 cut(s) 31, 334, 365, 578, 1054, 1170
BlsI GCNGC 6 cut(s) 32, 335, 366, 579, 1055, 1171
Bme18I GGWCC 2 cut(s) 670, 1270
BmgT120I GGNCC 2 cut(s) 670, 1270
BmiI GGNNCC 2 cut(s) 671, 1272
BmsI GCATC 3 cut(s) 179, 610, 730
BmtI GCTAGC 1 cut(s) 371
BplI GAGNNNNNCTC 4 cut(s) 44, 76, 276, 308
BsaJI CCNNGG 1 cut(s) 888
BsaWI WCCGGW 3 cut(s) 281, 827, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 666, 1279
Bse118I RCCGGY 3 cut(s) 299, 785, 1150
Bse3DI GCAATG 2 cut(s) 733, 772
BseAI TCCGGA 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 888
BseGI GGATG 3 cut(s) 89, 305, 984
BseLI CCNNNNNNNGG 2 cut(s) 666, 1279
BseMI GCAATG 2 cut(s) 733, 772
BseRI GAGGAG 7 cut(s) 85, 88, 133, 244, 247, 337, 1102
BseXI GCAGC 3 cut(s) 320, 351, 1065
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 1072
Bsh1236I CGCG 1 cut(s) 929
Bsh1285I CGRYCG 2 cut(s) 941, 1151
BshTI ACCGGT 1 cut(s) 1150
BsiEI CGRYCG 2 cut(s) 941, 1151
BsiSI CCGG 7 cut(s) 282, 300, 574, 786, 828, 924, 1151
BslFI GGGAC 4 cut(s) 18, 161, 683, 1256
BslI CCNNNNNNNGG 2 cut(s) 666, 1279
BsmAI GTCTC 5 cut(s) 333, 399, 539, 651, 848
BsmBI CGTCTC 1 cut(s) 539
BsmFI GGGAC 4 cut(s) 18, 161, 683, 1256
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
BspEI TCCGGA 1 cut(s) 281
BspFNI CGCG 1 cut(s) 929
BspLI GGNNCC 2 cut(s) 671, 1272
BspMAI CTGCAG 1 cut(s) 338
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 733, 772
BsrFI RCCGGY 3 cut(s) 299, 785, 1150
BssAI RCCGGY 3 cut(s) 299, 785, 1150
BssECI CCNNGG 1 cut(s) 888
BssMI GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
BssNAI GTATAC 1 cut(s) 781
BssSI CACGAG 1 cut(s) 1117
BssT1I CCWWGG 1 cut(s) 888
Bst1107I GTATAC 1 cut(s) 781
Bst2BI CACGAG 1 cut(s) 1117
Bst4CI ACNGT 6 cut(s) 41, 388, 533, 546, 1187, 1244
Bst6I CTCTTC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 2 cut(s) 161, 998
BstF5I GGATG 3 cut(s) 89, 305, 984
BstFNI CGCG 1 cut(s) 929
BstH2I RGCGCY 2 cut(s) 429, 998
BstHHI GCGC 4 cut(s) 428, 733, 929, 997
BstKTI GATC 6 cut(s) 85, 326, 807, 937, 990, 1108
BstMAI GTCTC 5 cut(s) 333, 399, 539, 651, 848
BstMBI GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
BstMCI CGRYCG 2 cut(s) 941, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 620, 749, 884
BstNSI RCATGY 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 334
BstUI CGCG 1 cut(s) 929
BstV1I GCAGC 3 cut(s) 320, 351, 1065
BstZ17I GTATAC 1 cut(s) 781
BtsCI GGATG 3 cut(s) 89, 305, 984
BtsIMutI CAGTG 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 369
CfoI GCGC 4 cut(s) 428, 733, 929, 997
Cfr10I RCCGGY 3 cut(s) 299, 785, 1150
Cfr13I GGNCC 2 cut(s) 670, 1270
CseI GACGC 1 cut(s) 620
Csp6I GTAC 3 cut(s) 42, 197, 919
CspAI ACCGGT 1 cut(s) 1150
CviAII CATG 7 cut(s) 15, 194, 327, 505, 605, 666, 734
CviQI GTAC 3 cut(s) 42, 197, 919
DdeI CTNAG 2 cut(s) 161, 998
DpnI GATC 6 cut(s) 84, 325, 806, 936, 989, 1107
DpnII GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 2 cut(s) 809, 1079
Eco130I CCWWGG 1 cut(s) 888
Eco47I GGWCC 2 cut(s) 670, 1270
Eco47III AGCGCT 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 1270
EcoT14I CCWWGG 1 cut(s) 888
EcoT22I ATGCAT 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 888
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 7 cut(s) 18, 197, 330, 508, 608, 669, 737
FaqI GGGAC 4 cut(s) 18, 161, 683, 1256
FatI CATG 7 cut(s) 14, 193, 326, 504, 604, 665, 733
FblI GTMKAC 2 cut(s) 654, 780
Fnu4HI GCNGC 6 cut(s) 31, 334, 365, 578, 1054, 1170
FokI GGATG 3 cut(s) 76, 292, 991
Fsp4HI GCNGC 6 cut(s) 31, 334, 365, 578, 1054, 1170
FspAI RTGCGCAY 1 cut(s) 732
FspBI CTAG 4 cut(s) 63, 368, 398, 860
FspI TGCGCA 1 cut(s) 732
GlaI GCGC 4 cut(s) 427, 732, 928, 996
GluI GCNGC 6 cut(s) 31, 334, 365, 578, 1054, 1170
GsaI CCCAGC 1 cut(s) 115
HaeII RGCGCY 2 cut(s) 429, 998
HapII CCGG 7 cut(s) 282, 300, 574, 786, 828, 924, 1151
HgaI GACGC 1 cut(s) 620
HhaI GCGC 4 cut(s) 428, 733, 929, 997
Hin1II CATG 7 cut(s) 18, 197, 330, 508, 608, 669, 737
Hin6I GCGC 4 cut(s) 426, 731, 927, 995
HinP1I GCGC 4 cut(s) 426, 731, 927, 995
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HinfI GANTC 6 cut(s) 47, 53, 251, 644, 716, 1211
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 7 cut(s) 282, 300, 574, 786, 828, 924, 1151
HphI GGTGA 7 cut(s) 422, 772, 817, 892, 1112, 1165, 1199
Hpy166II GTNNAC 5 cut(s) 591, 610, 655, 781, 883
Hpy188I TCNGA 7 cut(s) 52, 82, 250, 310, 700, 904, 1110
Hpy188III TCNNGA 2 cut(s) 241, 282
Hpy8I GTNNAC 5 cut(s) 591, 610, 655, 781, 883
Hpy99I CGWCG 3 cut(s) 314, 569, 908
HpyAV CCTTC 5 cut(s) 96, 144, 631, 1130, 1246
HpyCH4III ACNGT 6 cut(s) 41, 388, 533, 546, 1187, 1244
HpyCH4IV ACGT 3 cut(s) 564, 593, 677
HpyCH4V TGCA 7 cut(s) 170, 231, 336, 498, 504, 601, 1053
HpyF10VI GCNNNNNNNGC 3 cut(s) 620, 749, 884
HpyF3I CTNAG 2 cut(s) 161, 998
HpySE526I ACGT 3 cut(s) 564, 593, 677
Hsp92II CATG 7 cut(s) 18, 197, 330, 508, 608, 669, 737
HspAI GCGC 4 cut(s) 426, 731, 927, 995
Kpn2I TCCGGA 1 cut(s) 281
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
LmnI GCTCC 2 cut(s) 330, 905
Lsp1109I GCAGC 3 cut(s) 320, 351, 1065
LweI GCATC 3 cut(s) 179, 610, 730
MaeI CTAG 4 cut(s) 63, 368, 398, 860
MaeII ACGT 3 cut(s) 564, 593, 677
MaeIII GTNAC 4 cut(s) 65, 180, 1112, 1244
MalI GATC 6 cut(s) 84, 325, 806, 936, 989, 1107
MboI GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
MboII GAAGA 7 cut(s) 99, 122, 144, 412, 749, 814, 829
MluCI AATT 2 cut(s) 458, 1285
MlyI GAGTC 4 cut(s) 62, 245, 653, 725
MmeI TCCRAC 6 cut(s) 81, 273, 333, 723, 927, 1113
Mph1103I ATGCAT 1 cut(s) 506
MroI TCCGGA 1 cut(s) 281
MseI TTAA 4 cut(s) 590, 1163, 1284, 1294
MslI CAYNNNNRTG 2 cut(s) 738, 1253
MspA1I CMGCKG 1 cut(s) 1056
MspI CCGG 7 cut(s) 282, 300, 574, 786, 828, 924, 1151
MvnI CGCG 1 cut(s) 929
MwoI GCNNNNNNNGC 3 cut(s) 620, 749, 884
NdeII GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 7 cut(s) 18, 197, 330, 508, 608, 669, 737
NlaIV GGNNCC 2 cut(s) 671, 1272
NmeAIII GCCGAG 1 cut(s) 770
NmuCI GTSAC 2 cut(s) 180, 1244
NsbI TGCGCA 1 cut(s) 732
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 1253
PciI ACATGT 1 cut(s) 193
PfeI GAWTC 2 cut(s) 47, 1211
PinAI ACCGGT 1 cut(s) 1150
PkrI GCNGC 6 cut(s) 32, 335, 366, 579, 1055, 1171
PleI GAGTC 4 cut(s) 61, 245, 652, 724
PpsI GAGTC 4 cut(s) 61, 245, 652, 724
PpuMI RGGWCCY 1 cut(s) 1270
PscI ACATGT 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 1270
PspFI CCCAGC 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 671, 1272
PspPI GGNCC 2 cut(s) 670, 1270
PspPPI RGGWCCY 1 cut(s) 1270
PstI CTGCAG 1 cut(s) 338
RsaI GTAC 3 cut(s) 43, 198, 920
RsaNI GTAC 3 cut(s) 42, 197, 919
RseI CAYNNNNRTG 2 cut(s) 738, 1253
SaqAI TTAA 4 cut(s) 590, 1163, 1284, 1294
SatI GCNGC 6 cut(s) 31, 334, 365, 578, 1054, 1170
Sau3AI GATC 6 cut(s) 82, 323, 804, 934, 987, 1105
Sau96I GGNCC 2 cut(s) 670, 1270
SchI GAGTC 4 cut(s) 62, 245, 653, 725
SfaNI GCATC 3 cut(s) 179, 610, 730
SfcI CTRYAG 1 cut(s) 334
SinI GGWCC 2 cut(s) 670, 1270
SmiMI CAYNNNNRTG 2 cut(s) 738, 1253
Sse9I AATT 2 cut(s) 458, 1285
SspI AATATT 1 cut(s) 1020
SspMI CTAG 4 cut(s) 63, 368, 398, 860
StyI CCWWGG 1 cut(s) 888
TaaI ACNGT 6 cut(s) 41, 388, 533, 546, 1187, 1244
TaiI ACGT 3 cut(s) 567, 596, 680
TaqI TCGA 4 cut(s) 462, 567, 635, 1289
TaqII GACCGA 1 cut(s) 927
TasI AATT 2 cut(s) 458, 1285
TatI WGTACW 3 cut(s) 41, 196, 918
TauI GCSGC 3 cut(s) 33, 580, 1172
TfiI GAWTC 2 cut(s) 47, 1211
Tru1I TTAA 4 cut(s) 590, 1163, 1284, 1294
Tru9I TTAA 4 cut(s) 590, 1163, 1284, 1294
TscAI CASTG 1 cut(s) 528
TseFI GTSAC 2 cut(s) 180, 1244
TseI GCWGC 3 cut(s) 333, 364, 1053
Tsp45I GTSAC 2 cut(s) 180, 1244
TspDTI ATGAA 4 cut(s) 78, 265, 654, 750
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 1 cut(s) 528
VpaK11BI GGWCC 2 cut(s) 670, 1270
XapI RAATTY 1 cut(s) 458
XceI RCATGY 1 cut(s) 197
XmiI GTMKAC 2 cut(s) 654, 780
XspI CTAG 4 cut(s) 63, 368, 398, 860
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.