Rroxscaffold_5G00385270

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015016
Physical Location & Seq
Reverse (-)
64365389 .. 64366630
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385270.1

Sequence Viewer

Length: 1242 bp
ATGGGACTTGTTCCATGTCCTTTGCCCTGCAGCACATACAGTACAGAATCCGAGTCCAACTTCAGTTACCACCTTCACTCTGATCCTTCAACCTCCTCAATATCTTCCCAACCAAGTCTTCCTTCTGTTCCTTCGCTATCACGGCAAGTACTACAGCAAACCCAAACCACCCAACTTAACTGCATAGCTACCCGAAAACCCGGCTCCGCCTGTGTCTGTTCCCTAGTCCTCGCCGGAAAGTTCCTATACACCGGCTCATCCGACGGCGACATTCGATCATGGAGCTGCAGAGACATCTCCTCAAACACTTCCAAGCCACTAGCTACCACCAACTCCACGGTCAAGTCTCTAGTGATGGTGGGCGACAAAATTTTCAGCGCTCACCAAGACCACAAAATCCGTGTTTGGCAAGTCAACACTACCAATGAACAGAAGTTCAAATGCAAATGCATAGCTACGCTGCCGACACTGAAAGATCGTTTCCCGAGGTTCTTCTTCCCCAACAGTTACGTCCAAGTCCGGCGGCACAAGAAATTAACGTGGGTGCATCATGCGGACGCAATATCAGCTCTGGCGGTATCAAAGGACGAGTCTCTTCTATACTCGGCTTCATGGGACCGCACGTTCAAAATCTGGCGGACCTCCGACTTCAAGTGCTTGGAGTCGGTGAGCAACGCCCACGAGGATGCCATAAACGCAATAGTGGTGAGCAATGATGGGTTTGTTTACACTGGCTCGGCGGATAAGAAGATAAATGTGTGGAAGAAACTCATTCTGGAAAAGCATCACACTCTTGTTGAGTCACTAGAGAAGCACAAGTCAGCGGTGAACGCCTTGGCTTTTAGCTCCGACGGGTCTGTGTTGTACTCCGGCGCGTGTGATCGGTCGATTCTGGTTTGGGAAAGAGATAATGGTGGCGGTGGTGGGATGGTTGTGGTGGGGGCGCTTAGAGGTCACACTAAGGCTATATTGTGTTTGGCGGTGGCGGGGAATTTAGTGTGTAGTGGTTCGGCGGATTGTAGTGTTAGGATTTGGAGGAGAGAGGTTGGTGATGATCGGAGTTACTCGTGTTTGGGTGTGTTGGAAGGTCATAGGCGGCCTGTGAAGTGTTTGACGGCAGCCGTGGATTTTGACGGTGACGATAGAAGTGATTGTGTTAATTCTTTTCTTGTTTATAGTGGTAGTTTGGATTGTAACATTAAGGTGTGGCAAATACAGGTCCCTTTGGTTAATTCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

413

Amino Acids

45.34

Weight (kDa)

8.68

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 28 - 304 1.7e-10 SCAP Beta-propeller
WD40_Prp19 PF24814 64 - 301 1.2e-16 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 72 - 144 9.8e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 175 - 374 1.5e-23 THOC3 beta-propeller domain
WDR55 PF24796 176 - 254 9.9e-09 WDR55
WD40_Gbeta PF25391 177 - 345 1e-11 G protein beta WD-40 repeat protein
WD40 PF00400 180 - 212 2.9e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 182 - 304 7.9e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 183 - 305 4.8e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 183 - 255 1.9e-09 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 183 - 301 2.2e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 184 - 348 1.1e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 186 - 303 5.2e-14 WDR3 second beta-propeller domain
NBCH_WD40 PF20426 187 - 257 4.4e-06 Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd PF25047 190 - 301 1.4e-09 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 190 - 348 4.7e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 204 - 346 9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 225 - 406 2.4e-25 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 238 - 301 2.3e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 244 - 350 2.1e-08 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 261 - 345 6.7e-07 WDR55
Beta-prop_WDR3_1st PF25173 265 - 301 2.9e-06 WDR3 first beta-propeller domain
WD40 PF00400 265 - 300 5.6e-08 WD domain, G-beta repeat
EIF3I PF24805 269 - 351 5e-08 EIF3I
WD40 PF00400 313 - 345 5.7e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 315 - 376 3e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 315 - 406 1.5e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24130
fragaria_vesca FvH4_4g35150
malus_domestica MD13G1085800.v1.1 MD16G1085100.v1.1
prunus_persica Prupe.1G260900_v2.0.a1
pyrus_communis pycom13g07420 pycom16g07360
rosa_chinensis RchiOBHm_Chr4g0444751
rosa_laevigata RLG00000005805
rosa_multiflora Rmu_sc0000096.1_g000011
rosa_roxburghii Rroxscaffold_5G00385270
rosa_samantha Rh4AG415800 Rh4BG427100 Rh4CG442000 Rh4DG423100
rosa_wichuraiana Rw4G035680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 875
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 369, 991
AcuI CTGAAG 1 cut(s) 46
AfaI GTAC 3 cut(s) 43, 150, 866
AfeI AGCGCT 1 cut(s) 379
AgsI TTSAA 4 cut(s) 90, 439, 628, 652
AjuI GAANNNNNNNTTGG 4 cut(s) 102, 106, 134, 138
AloI GAACNNNNNNTCC 2 cut(s) 608, 640
AluBI AGCT 6 cut(s) 188, 285, 323, 455, 569, 846
AluI AGCT 6 cut(s) 188, 285, 323, 455, 569, 846
Alw26I GTCTC 3 cut(s) 285, 351, 597
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 484
Aor51HI AGCGCT 1 cut(s) 379
AoxI GGCC 1 cut(s) 1097
ApeKI GCWGC 4 cut(s) 30, 285, 460, 1118
ApoI RAATTY 2 cut(s) 369, 991
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AspLEI GCGC 3 cut(s) 380, 875, 946
AspS9I GGNCC 3 cut(s) 616, 639, 1219
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 6 cut(s) 374, 679, 718, 838, 1061, 1148
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 3 cut(s) 616, 639, 1219
BauI CACGAG 2 cut(s) 680, 1066
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 4 cut(s) 42, 272, 447, 1130
BccI CCATC 3 cut(s) 349, 710, 922
BceAI ACGGC 4 cut(s) 158, 280, 1106, 1131
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 3 cut(s) 285, 351, 597
BfaI CTAG 4 cut(s) 224, 320, 350, 806
BfmI CTRYAG 3 cut(s) 28, 152, 286
BfoI RGCGCY 2 cut(s) 381, 947
BisI GCNGC 6 cut(s) 31, 286, 461, 524, 1097, 1119
BlsI GCNGC 6 cut(s) 32, 287, 462, 525, 1098, 1120
BmcAI AGTACT 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 3 cut(s) 616, 639, 1219
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 3 cut(s) 616, 639, 1219
BmiI GGNNCC 3 cut(s) 205, 617, 1221
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 3 cut(s) 556, 676, 793
BpiI GAAGAC 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 201
BsaJI CCNNGG 4 cut(s) 336, 485, 834, 1122
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 736
Bse3DI GCAATG 1 cut(s) 718
BseDI CCNNGG 4 cut(s) 336, 485, 834, 1122
BseGI GGATG 3 cut(s) 257, 691, 933
BseMI GCAATG 1 cut(s) 718
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 3 cut(s) 85, 289, 1051
BseXI GCAGC 4 cut(s) 42, 272, 447, 1130
Bsh1236I CGCG 1 cut(s) 875
Bsh1285I CGRYCG 1 cut(s) 887
BshFI GGCC 1 cut(s) 1099
BsiEI CGRYCG 1 cut(s) 887
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 5 cut(s) 201, 234, 252, 520, 870
BslFI GGGAC 3 cut(s) 18, 629, 1205
BsmAI GTCTC 3 cut(s) 285, 351, 597
BsmFI GGGAC 3 cut(s) 18, 629, 1205
BsnI GGCC 1 cut(s) 1099
BsoBI CYCGRG 1 cut(s) 484
Bsp143I GATC 5 cut(s) 82, 275, 475, 880, 1054
BspANI GGCC 1 cut(s) 1099
BspFNI CGCG 1 cut(s) 875
BspLI GGNNCC 3 cut(s) 205, 617, 1221
BspMAI CTGCAG 2 cut(s) 32, 290
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 718
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 736
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 4 cut(s) 336, 485, 834, 1122
BssMI GATC 5 cut(s) 82, 275, 475, 880, 1054
BssSI CACGAG 2 cut(s) 680, 1066
BssT1I CCWWGG 1 cut(s) 834
Bst2BI CACGAG 2 cut(s) 680, 1066
Bst4CI ACNGT 4 cut(s) 41, 340, 506, 1136
Bst6I CTCTTC 1 cut(s) 600
BstDEI CTNAG 2 cut(s) 947, 960
BstDSI CCRYGG 2 cut(s) 336, 1122
BstF5I GGATG 3 cut(s) 257, 691, 933
BstFNI CGCG 1 cut(s) 875
BstH2I RGCGCY 2 cut(s) 381, 947
BstHHI GCGC 3 cut(s) 380, 875, 946
BstKTI GATC 5 cut(s) 85, 278, 478, 883, 1057
BstMAI GTCTC 3 cut(s) 285, 351, 597
BstMBI GATC 5 cut(s) 82, 275, 475, 880, 1054
BstMCI CGRYCG 1 cut(s) 887
BstMWI GCNNNNNNNGC 4 cut(s) 142, 566, 695, 830
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 3 cut(s) 28, 152, 286
BstUI CGCG 1 cut(s) 875
BstV1I GCAGC 4 cut(s) 42, 272, 447, 1130
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 1099
BtgI CCRYGG 2 cut(s) 336, 1122
BtsCI GGATG 3 cut(s) 257, 691, 933
BtsIMutI CAGTG 2 cut(s) 467, 729
CfoI GCGC 3 cut(s) 380, 875, 946
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 616, 639, 1219
CseI GACGC 1 cut(s) 566
Csp6I GTAC 3 cut(s) 42, 149, 865
CviAII CATG 4 cut(s) 15, 279, 551, 612
CviQI GTAC 3 cut(s) 42, 149, 865
DdeI CTNAG 2 cut(s) 947, 960
DpnI GATC 5 cut(s) 84, 277, 477, 882, 1056
DpnII GATC 5 cut(s) 82, 275, 475, 880, 1054
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
EciI GGCGGA 4 cut(s) 196, 652, 755, 1028
Eco130I CCWWGG 1 cut(s) 834
Eco47I GGWCC 3 cut(s) 616, 639, 1219
Eco47III AGCGCT 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 1219
EcoT14I CCWWGG 1 cut(s) 834
EcoT22I ATGCAT 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 834
FaeI CATG 4 cut(s) 18, 282, 554, 615
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 3 cut(s) 18, 629, 1205
FatI CATG 4 cut(s) 14, 278, 550, 611
FauI CCCGC 1 cut(s) 979
Fnu4HI GCNGC 6 cut(s) 31, 286, 461, 524, 1097, 1119
FokI GGATG 3 cut(s) 244, 698, 940
Fsp4HI GCNGC 6 cut(s) 31, 286, 461, 524, 1097, 1119
FspBI CTAG 4 cut(s) 224, 320, 350, 806
GlaI GCGC 3 cut(s) 379, 874, 945
GluI GCNGC 6 cut(s) 31, 286, 461, 524, 1097, 1119
HaeII RGCGCY 2 cut(s) 381, 947
HaeIII GGCC 1 cut(s) 1099
HapII CCGG 5 cut(s) 201, 234, 252, 520, 870
HgaI GACGC 1 cut(s) 566
HhaI GCGC 3 cut(s) 380, 875, 946
Hin1II CATG 4 cut(s) 18, 282, 554, 615
Hin6I GCGC 3 cut(s) 378, 873, 944
HinP1I GCGC 3 cut(s) 378, 873, 944
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 6 cut(s) 47, 53, 590, 662, 800, 889
HpaII CCGG 5 cut(s) 201, 234, 252, 520, 870
HphI GGTGA 6 cut(s) 374, 679, 718, 838, 1061, 1148
Hpy166II GTNNAC 3 cut(s) 415, 727, 829
Hpy188I TCNGA 6 cut(s) 52, 82, 262, 646, 850, 1059
Hpy188III TCNNGA 2 cut(s) 484, 776
Hpy8I GTNNAC 3 cut(s) 415, 727, 829
Hpy99I CGWCG 2 cut(s) 266, 854
HpyAV CCTTC 5 cut(s) 83, 96, 132, 141, 1079
HpyCH4III ACNGT 4 cut(s) 41, 340, 506, 1136
HpyCH4IV ACGT 3 cut(s) 510, 539, 623
HpyCH4V TGCA 6 cut(s) 30, 183, 288, 444, 450, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 566, 695, 830
HpyF3I CTNAG 2 cut(s) 947, 960
HpySE526I ACGT 3 cut(s) 510, 539, 623
Hsp92II CATG 4 cut(s) 18, 282, 554, 615
HspAI GCGC 3 cut(s) 378, 873, 944
Kzo9I GATC 5 cut(s) 82, 275, 475, 880, 1054
LmnI GCTCC 3 cut(s) 209, 282, 851
Lsp1109I GCAGC 4 cut(s) 42, 272, 447, 1130
LweI GCATC 3 cut(s) 556, 676, 793
MaeI CTAG 4 cut(s) 224, 320, 350, 806
MaeII ACGT 3 cut(s) 510, 539, 623
MaeIII GTNAC 7 cut(s) 65, 506, 801, 953, 1061, 1136, 1193
MalI GATC 5 cut(s) 84, 277, 477, 882, 1056
MboI GATC 5 cut(s) 82, 275, 475, 880, 1054
MboII GAAGA 7 cut(s) 96, 110, 484, 487, 587, 760, 775
MluCI AATT 5 cut(s) 369, 533, 991, 1159, 1231
MlyI GAGTC 4 cut(s) 62, 599, 671, 809
MmeI TCCRAC 5 cut(s) 81, 285, 669, 873, 1062
Mph1103I ATGCAT 1 cut(s) 452
MseI TTAA 6 cut(s) 177, 536, 1158, 1200, 1230, 1240
MslI CAYNNNNRTG 2 cut(s) 684, 1202
MspA1I CMGCKG 1 cut(s) 824
MspI CCGG 5 cut(s) 201, 234, 252, 520, 870
MspR9I CCNGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 875
MwoI GCNNNNNNNGC 4 cut(s) 142, 566, 695, 830
NciI CCSGG 1 cut(s) 201
NdeII GATC 5 cut(s) 82, 275, 475, 880, 1054
NlaIII CATG 4 cut(s) 18, 282, 554, 615
NlaIV GGNNCC 3 cut(s) 205, 617, 1221
NmeAIII GCCGAG 2 cut(s) 584, 716
NmuCI GTSAC 3 cut(s) 801, 953, 1136
NsiI ATGCAT 1 cut(s) 452
PfeI GAWTC 2 cut(s) 47, 889
PkrI GCNGC 6 cut(s) 32, 287, 462, 525, 1098, 1120
PleI GAGTC 4 cut(s) 61, 598, 670, 808
PpsI GAGTC 4 cut(s) 61, 598, 670, 808
PpuMI RGGWCCY 1 cut(s) 1219
Psp5II RGGWCCY 1 cut(s) 1219
PspN4I GGNNCC 3 cut(s) 205, 617, 1221
PspPI GGNCC 3 cut(s) 616, 639, 1219
PspPPI RGGWCCY 1 cut(s) 1219
PstI CTGCAG 2 cut(s) 32, 290
RsaI GTAC 3 cut(s) 43, 150, 866
RsaNI GTAC 3 cut(s) 42, 149, 865
RseI CAYNNNNRTG 2 cut(s) 684, 1202
SaqAI TTAA 6 cut(s) 177, 536, 1158, 1200, 1230, 1240
SatI GCNGC 6 cut(s) 31, 286, 461, 524, 1097, 1119
Sau3AI GATC 5 cut(s) 82, 275, 475, 880, 1054
Sau96I GGNCC 3 cut(s) 616, 639, 1219
ScaI AGTACT 1 cut(s) 150
SchI GAGTC 4 cut(s) 62, 599, 671, 809
ScrFI CCNGG 1 cut(s) 201
SfaNI GCATC 3 cut(s) 556, 676, 793
SfcI CTRYAG 3 cut(s) 28, 152, 286
SinI GGWCC 3 cut(s) 616, 639, 1219
SmiMI CAYNNNNRTG 2 cut(s) 684, 1202
Sse9I AATT 5 cut(s) 369, 533, 991, 1159, 1231
SspMI CTAG 4 cut(s) 224, 320, 350, 806
StyD4I CCNGG 1 cut(s) 199
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 4 cut(s) 41, 340, 506, 1136
TaiI ACGT 3 cut(s) 513, 542, 626
TaqI TCGA 3 cut(s) 274, 887, 1235
TaqII GACCGA 1 cut(s) 873
TasI AATT 5 cut(s) 369, 533, 991, 1159, 1231
TatI WGTACW 3 cut(s) 41, 148, 864
TauI GCSGC 2 cut(s) 526, 1099
TfiI GAWTC 2 cut(s) 47, 889
Tru1I TTAA 6 cut(s) 177, 536, 1158, 1200, 1230, 1240
Tru9I TTAA 6 cut(s) 177, 536, 1158, 1200, 1230, 1240
TscAI CASTG 2 cut(s) 474, 736
TseFI GTSAC 3 cut(s) 801, 953, 1136
TseI GCWGC 4 cut(s) 30, 285, 460, 1118
Tsp45I GTSAC 3 cut(s) 801, 953, 1136
TspDTI ATGAA 2 cut(s) 441, 600
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 474, 736
VpaK11BI GGWCC 3 cut(s) 616, 639, 1219
XapI RAATTY 2 cut(s) 369, 991
XspI CTAG 4 cut(s) 224, 320, 350, 806
ZrmI AGTACT 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.