MD16G1085100.v1.1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015016
Physical Location & Seq
Forward (+)
5959132 .. 5960439
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1085100.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGGGACTCGTTCCATGTCCCTTGCCCTGTCACACAAGCACAGAAAGTAAGGAAGAATCTTCAACCTCCAACTCCACCTCCAGTCTTCTCTCTGAGTCTTCAACCTTCTCCTCTCTGTCCTCCCAGCCAAGTCTCCCTTCTGTTCCTTCTCTCACCCCATCAAAATCCAACCAATTAGTAGTACTACGATCCTCAAGTGTTCATCACTGCTGCATAGCCACTCTCCAATCCCAGTCCTACATCTGTACCCTAGCCCTATCTGGAAACTTCCTATACAGTGGCTCATCCGACGGTCAGATTACAGCATGGAGTAGTACCCTATCACGCCCTTCAAATCCAAAATACAACAAGGTGGTAGCCACCGTCGCCACTCCCAGCACCGTCAAGTTTCTGGTGGTTGTTGGGAGTGGGGACGACCGCAACAAGCTCTTATTCGGTGCTCACCAAGACCATAAAATCCGAGTCTGGAAAACTAACACGAACGACACGTATGAAAAACTAAAATGTATAGCCACACTCCCAACTCTAAAAGAACGTGTCACAAGATTCTTATGTTCCAAGAACTACGTGCAAATCGGGCTCCACAAGAACGGCACCTGGGAGCATCACATCGACGCCGTCTCCGCTCTAGCGATATCCAACGACGGGTCGTTTCTCTACTCCGTTTCATGGGACCGGGCGCTCAAAATATGGCGGACTTCCGACTTTAAATGCATGGACTCGGTTAGTAACGCCCACGACGACGCCATCAACGCCGTAATTTTGTCAAGTGACGGAGCTTTTGTGTACACCGGCTCGGCGGATAAGAAGATAAAGGTGTGGACGTGGAGGAAAGACAAGTCAACATCAGTAAAGCTTGTTGGAACCCTAGAGAAGCACAAGTCGGCTGTGAATGCGTTGGCTTTTAGCACCGACGGGTCCGTGCTGTACTCTGGTGCCTGCGACCGGTCGGTTTTGGTTTGGGAAAGAGATGGTGGTGCGGACGGCGGCGGTGGGGACATGGTGGTGGTCGGTGCGTTGAGGGGACACACACAGGCGGTGTTGTGTTTGGCGGTGGCGGCTGATTTGTTGTGCAGTGGATCTGCTGATTATAGTGTTAGGGTTTGGAGGAGATTATCGTCAGGGCTAGTTGGCGGTGATCTAAATAGGAGTTATTGCTGTCTGGCTGTGTTGGAAGGTCACAAGCGGCCGGTGAAGTGTTTGACTGCAGCTCTTGATAATAGTGATTCTGGTGATTCTTATTTTGTTTATAGTGGCAGCTTGGATTGTGATATTAAGGTCTGGCAAATTCGGGTTCCCCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

436

Amino Acids

46.86

Weight (kDa)

8.53

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 52 - 231 3.8e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 87 - 322 5.7e-17 Prp19 WD40 domain
WD40_Gbeta PF25391 191 - 369 2.2e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 191 - 322 4.6e-06 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 197 - 281 7e-11 WDR55
WD40 PF00400 198 - 231 6.1e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 201 - 324 5.4e-23 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 202 - 275 3.2e-10 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 203 - 373 1.2e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 203 - 283 2.7e-10 THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 205 - 322 9.6e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML_2 PF23414 205 - 325 4.7e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 207 - 323 1.5e-14 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 236 - 322 6.6e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 244 - 274 1.2e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 249 - 322 4.4e-07 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 264 - 370 6.1e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 284 - 369 9.6e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 284 - 430 6.4e-17 WDR5 beta-propeller domain
WD40 PF00400 285 - 321 2.5e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 293 - 370 2.3e-11 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 339 - 433 3.5e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 339 - 430 1.4e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24130
fragaria_vesca FvH4_4g35150
malus_domestica MD13G1085800.v1.1 MD16G1085100.v1.1
prunus_persica Prupe.1G260900_v2.0.a1
pyrus_communis pycom13g07420 pycom16g07360
rosa_chinensis RchiOBHm_Chr4g0444751
rosa_laevigata RLG00000005805
rosa_multiflora Rmu_sc0000096.1_g000011
rosa_roxburghii Rroxscaffold_5G00385270
rosa_samantha Rh4AG415800 Rh4BG427100 Rh4CG442000 Rh4DG423100
rosa_wichuraiana Rw4G035680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 593, 935
AccBSI CCGCTC 1 cut(s) 626
AclWI GGATC 2 cut(s) 183, 1087
AcoI YGGCCR 1 cut(s) 1187
AcsI RAATTY 1 cut(s) 1287
AcyI GRCGYC 2 cut(s) 615, 744
AfaI GTAC 5 cut(s) 183, 247, 316, 788, 929
AfiI CCNNNNNNNGG 3 cut(s) 645, 669, 945
AflIII ACRYGT 2 cut(s) 486, 535
AgeI ACCGGT 1 cut(s) 945
AgsI TTSAA 3 cut(s) 63, 102, 333
AjiI CACGTC 1 cut(s) 825
AjnI CCWGG 1 cut(s) 596
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AluBI AGCT 5 cut(s) 427, 779, 856, 1211, 1260
AluI AGCT 5 cut(s) 427, 779, 856, 1211, 1260
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 2 cut(s) 137, 625
AlwI GGATC 2 cut(s) 183, 1087
AoxI GGCC 1 cut(s) 1187
ApeKI GCWGC 3 cut(s) 210, 1208, 1257
ApoI RAATTY 1 cut(s) 1287
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AsiGI ACCGGT 1 cut(s) 945
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 2 cut(s) 673, 918
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 5 cut(s) 145, 434, 1148, 1204, 1244
AvaII GGWCC 2 cut(s) 673, 918
BaeI ACNNNNGTAYC 2 cut(s) 229, 262
BanI GGYRCC 2 cut(s) 593, 935
BanII GRGCYC 1 cut(s) 582
BbsI GAAGAC 2 cut(s) 77, 90
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 3 cut(s) 197, 1220, 1269
BccI CCATC 3 cut(s) 166, 755, 965
BceAI ACGGC 4 cut(s) 602, 607, 740, 1000
BciT130I CCWGG 1 cut(s) 598
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 2 cut(s) 137, 625
BfaI CTAG 4 cut(s) 251, 629, 869, 1127
BfmI CTRYAG 1 cut(s) 1206
BfoI RGCGCY 1 cut(s) 683
BisI GCNGC 6 cut(s) 211, 988, 1059, 1187, 1209, 1258
BlsI GCNGC 6 cut(s) 212, 989, 1060, 1188, 1210, 1259
BmcAI AGTACT 1 cut(s) 183
Bme1390I CCNGG 2 cut(s) 598, 677
Bme18I GGWCC 2 cut(s) 673, 918
BmgBI CACGTC 1 cut(s) 825
BmgT120I GGNCC 2 cut(s) 673, 918
BmiI GGNNCC 7 cut(s) 581, 595, 674, 865, 919, 937, 1296
BmrFI CCNGG 2 cut(s) 598, 677
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 226
BpiI GAAGAC 2 cut(s) 77, 90
BpmI CTGGAG 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 677
BsaAI YACGTR 2 cut(s) 489, 568
BsaHI GRCGYC 2 cut(s) 615, 744
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 945
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 605, 635, 768, 798
Bsc4I CCNNNNNNNGG 3 cut(s) 645, 669, 945
Bse118I RCCGGY 3 cut(s) 791, 945, 1189
Bse1I ACTGG 2 cut(s) 81, 232
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 3 cut(s) 645, 669, 945
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 81, 232
BseRI GAGGAG 2 cut(s) 100, 1123
BseX3I CGGCCG 1 cut(s) 1187
BseXI GCAGC 3 cut(s) 197, 1220, 1269
BseYI CCCAGC 2 cut(s) 123, 374
BsgI GTGCAG 1 cut(s) 1093
Bsh1285I CGRYCG 4 cut(s) 418, 946, 950, 1190
BshFI GGCC 1 cut(s) 1189
BshNI GGYRCC 2 cut(s) 593, 935
BshTI ACCGGT 1 cut(s) 945
BsiEI CGRYCG 4 cut(s) 418, 946, 950, 1190
BsiHKAI GWGCWC 1 cut(s) 442
BsiSI CCGG 4 cut(s) 676, 792, 946, 1190
BslFI GGGAC 6 cut(s) 3, 18, 425, 686, 1010, 1038
BslI CCNNNNNNNGG 3 cut(s) 645, 669, 945
BsmAI GTCTC 2 cut(s) 137, 625
BsmBI CGTCTC 1 cut(s) 625
BsmFI GGGAC 6 cut(s) 3, 18, 425, 686, 1010, 1038
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 1 cut(s) 1189
Bsp1286I GDGCHC 2 cut(s) 442, 582
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 3 cut(s) 188, 1079, 1138
BspANI GGCC 1 cut(s) 1189
BspCNI CTCAG 1 cut(s) 85
BspLI GGNNCC 7 cut(s) 581, 595, 674, 865, 919, 937, 1296
BspMAI CTGCAG 1 cut(s) 1210
BspPI GGATC 2 cut(s) 183, 1087
BspT107I GGYRCC 2 cut(s) 593, 935
BsrBI CCGCTC 1 cut(s) 626
BsrFI RCCGGY 3 cut(s) 791, 945, 1189
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 2 cut(s) 81, 232
BssAI RCCGGY 3 cut(s) 791, 945, 1189
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 3 cut(s) 188, 1079, 1138
BssNI GRCGYC 2 cut(s) 615, 744
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 4 cut(s) 278, 293, 364, 382
BstACI GRCGYC 2 cut(s) 615, 744
BstAUI TGTACA 1 cut(s) 786
BstBAI YACGTR 2 cut(s) 489, 568
BstC8I GCNNGC 1 cut(s) 940
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 3 cut(s) 191, 1082, 1141
BstMAI GTCTC 2 cut(s) 137, 625
BstMBI GATC 3 cut(s) 188, 1079, 1138
BstMCI CGRYCG 4 cut(s) 418, 946, 950, 1190
BstMWI GCNNNNNNNGC 6 cut(s) 365, 577, 623, 752, 893, 1058
BstNI CCWGG 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 596, 675
BstSFI CTRYAG 1 cut(s) 1206
BstV1I GCAGC 3 cut(s) 197, 1220, 1269
BstV2I GAAGAC 2 cut(s) 77, 90
BstX2I RGATCY 1 cut(s) 1079
BstYI RGATCY 1 cut(s) 1079
BstZI CGGCCG 1 cut(s) 1187
BsuRI GGCC 1 cut(s) 1189
BtrI CACGTC 1 cut(s) 825
BtsCI GGATG 1 cut(s) 284
BtsI GCAGTG 2 cut(s) 205, 1081
BtsIMutI CAGTG 3 cut(s) 205, 283, 1081
Cac8I GCNNGC 1 cut(s) 940
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 3 cut(s) 791, 945, 1189
Cfr13I GGNCC 2 cut(s) 673, 918
CseI GACGC 2 cut(s) 623, 752
Csp6I GTAC 5 cut(s) 182, 246, 315, 787, 928
CspAI ACCGGT 1 cut(s) 945
CviAII CATG 5 cut(s) 15, 306, 669, 715, 1000
CviQI GTAC 5 cut(s) 182, 246, 315, 787, 928
DdeI CTNAG 1 cut(s) 93
DpnI GATC 3 cut(s) 190, 1081, 1140
DpnII GATC 3 cut(s) 188, 1079, 1138
DraI TTTAAA 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 1187
EagI CGGCCG 1 cut(s) 1187
EciI GGCGGA 2 cut(s) 709, 815
EclXI CGGCCG 1 cut(s) 1187
Eco24I GRGCYC 1 cut(s) 582
Eco32I GATATC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 673, 918
Eco52I CGGCCG 1 cut(s) 1187
EcoRII CCWGG 1 cut(s) 596
EcoRV GATATC 1 cut(s) 636
EcoT22I ATGCAT 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 625
FaeI CATG 5 cut(s) 18, 309, 672, 718, 1003
FalI AAGNNNNNCTT 2 cut(s) 121, 153
FaqI GGGAC 6 cut(s) 3, 18, 425, 686, 1010, 1038
FatI CATG 5 cut(s) 14, 305, 668, 714, 999
Fnu4HI GCNGC 6 cut(s) 211, 988, 1059, 1187, 1209, 1258
FokI GGATG 1 cut(s) 271
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 6 cut(s) 211, 988, 1059, 1187, 1209, 1258
FspBI CTAG 4 cut(s) 251, 629, 869, 1127
GlaI GCGC 1 cut(s) 681
GluI GCNGC 6 cut(s) 211, 988, 1059, 1187, 1209, 1258
GsaI CCCAGC 2 cut(s) 127, 378
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 1 cut(s) 1189
HapII CCGG 4 cut(s) 676, 792, 946, 1190
HgaI GACGC 2 cut(s) 623, 752
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 2 cut(s) 615, 744
Hin1II CATG 5 cut(s) 18, 309, 672, 718, 1003
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HincII GTYRAC 1 cut(s) 843
HindII GTYRAC 1 cut(s) 843
HindIII AAGCTT 1 cut(s) 854
HinfI GANTC 8 cut(s) 6, 56, 95, 462, 546, 719, 1226, 1235
HpaII CCGG 4 cut(s) 676, 792, 946, 1190
HphI GGTGA 5 cut(s) 145, 434, 1148, 1204, 1244
Hpy166II GTNNAC 4 cut(s) 787, 789, 822, 843
Hpy188I TCNGA 5 cut(s) 94, 289, 297, 461, 703
Hpy188III TCNNGA 3 cut(s) 261, 466, 1214
Hpy8I GTNNAC 4 cut(s) 787, 789, 822, 843
Hpy99I CGWCG 7 cut(s) 293, 368, 617, 647, 743, 746, 917
HpyAV CCTTC 5 cut(s) 115, 147, 156, 339, 1169
HpyCH4III ACNGT 4 cut(s) 278, 293, 364, 382
HpyCH4IV ACGT 4 cut(s) 488, 535, 567, 824
HpyCH4V TGCA 5 cut(s) 213, 571, 714, 1074, 1208
HpyF10VI GCNNNNNNNGC 6 cut(s) 365, 577, 623, 752, 893, 1058
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 4 cut(s) 488, 535, 567, 824
Hsp92I GRCGYC 2 cut(s) 615, 744
Hsp92II CATG 5 cut(s) 18, 309, 672, 718, 1003
HspAI GCGC 1 cut(s) 680
Kzo9I GATC 3 cut(s) 188, 1079, 1138
LmnI GCTCC 3 cut(s) 585, 601, 776
Lsp1109I GCAGC 3 cut(s) 197, 1220, 1269
LweI GCATC 1 cut(s) 613
MaeI CTAG 4 cut(s) 251, 629, 869, 1127
MaeII ACGT 4 cut(s) 488, 535, 567, 824
MaeIII GTNAC 5 cut(s) 29, 538, 728, 770, 1178
MalI GATC 3 cut(s) 190, 1081, 1140
MbiI CCGCTC 1 cut(s) 626
MboI GATC 3 cut(s) 188, 1079, 1138
MboII GAAGA 5 cut(s) 51, 65, 77, 90, 820
MflI RGATCY 1 cut(s) 1079
MhlI GDGCHC 2 cut(s) 442, 582
MluCI AATT 3 cut(s) 173, 759, 1287
MlyI GAGTC 3 cut(s) 104, 471, 713
MmeI TCCRAC 7 cut(s) 93, 192, 312, 663, 726, 841, 1152
MnlI CCTC 8 cut(s) 76, 88, 121, 130, 202, 822, 1014, 1101
Mph1103I ATGCAT 1 cut(s) 716
MseI TTAA 2 cut(s) 708, 1275
MslI CAYNNNNRTG 1 cut(s) 1004
MspI CCGG 4 cut(s) 676, 792, 946, 1190
MspR9I CCNGG 2 cut(s) 598, 677
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 598
MwoI GCNNNNNNNGC 6 cut(s) 365, 577, 623, 752, 893, 1058
NciI CCSGG 1 cut(s) 677
NdeII GATC 3 cut(s) 188, 1079, 1138
NlaIII CATG 5 cut(s) 18, 309, 672, 718, 1003
NlaIV GGNNCC 7 cut(s) 581, 595, 674, 865, 919, 937, 1296
NmeAIII GCCGAG 1 cut(s) 776
NmuCI GTSAC 4 cut(s) 29, 538, 770, 1178
NsiI ATGCAT 1 cut(s) 716
PcsI WCGNNNNNNNCGW 2 cut(s) 485, 738
PctI GAATGC 1 cut(s) 898
PfeI GAWTC 4 cut(s) 56, 546, 1226, 1235
PflFI GACNNNGTC 1 cut(s) 617
PinAI ACCGGT 1 cut(s) 945
PkrI GCNGC 6 cut(s) 212, 989, 1060, 1188, 1210, 1259
PleI GAGTC 3 cut(s) 103, 470, 713
PpsI GAGTC 3 cut(s) 103, 470, 713
Ppu21I YACGTR 2 cut(s) 489, 568
Psp6I CCWGG 1 cut(s) 596
PspFI CCCAGC 2 cut(s) 123, 374
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 7 cut(s) 581, 595, 674, 865, 919, 937, 1296
PspPI GGNCC 2 cut(s) 673, 918
PstI CTGCAG 1 cut(s) 1210
PsuI RGATCY 1 cut(s) 1079
PsyI GACNNNGTC 1 cut(s) 617
RsaI GTAC 5 cut(s) 183, 247, 316, 788, 929
RsaNI GTAC 5 cut(s) 182, 246, 315, 787, 928
RseI CAYNNNNRTG 1 cut(s) 1004
SaqAI TTAA 2 cut(s) 708, 1275
SatI GCNGC 6 cut(s) 211, 988, 1059, 1187, 1209, 1258
Sau3AI GATC 3 cut(s) 188, 1079, 1138
Sau96I GGNCC 2 cut(s) 673, 918
ScaI AGTACT 1 cut(s) 183
SchI GAGTC 3 cut(s) 104, 471, 713
ScrFI CCNGG 2 cut(s) 598, 677
SduI GDGCHC 2 cut(s) 442, 582
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 1206
SinI GGWCC 2 cut(s) 673, 918
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 3 cut(s) 173, 759, 1287
SspMI CTAG 4 cut(s) 251, 629, 869, 1127
StyD4I CCNGG 2 cut(s) 596, 675
TaaI ACNGT 4 cut(s) 278, 293, 364, 382
TaiI ACGT 4 cut(s) 491, 538, 570, 827
TaqI TCGA 1 cut(s) 612
TasI AATT 3 cut(s) 173, 759, 1287
TatI WGTACW 3 cut(s) 181, 786, 927
TauI GCSGC 3 cut(s) 990, 1061, 1189
TfiI GAWTC 4 cut(s) 56, 546, 1226, 1235
Tru1I TTAA 2 cut(s) 708, 1275
Tru9I TTAA 2 cut(s) 708, 1275
TscAI CASTG 3 cut(s) 212, 283, 1081
TseFI GTSAC 4 cut(s) 29, 538, 770, 1178
TseI GCWGC 3 cut(s) 210, 1208, 1257
Tsp45I GTSAC 4 cut(s) 29, 538, 770, 1178
TspDTI ATGAA 4 cut(s) 191, 507, 657, 1293
TspGWI ACGGA 3 cut(s) 652, 789, 910
TspRI CASTG 3 cut(s) 212, 283, 1081
Tth111I GACNNNGTC 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 673, 918
XapI RAATTY 1 cut(s) 1287
XspI CTAG 4 cut(s) 251, 629, 869, 1127
ZrmI AGTACT 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.