FvH4_4g35480

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008071
Physical Location & Seq
Forward (+)
32789565 .. 32794760
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35480.t1

Sequence Viewer

Length: 1704 bp
ATGGGGTCGTGTGTGTCGACGCCGGACCGGTGCGTCGGAAGGAGGCTGAGTTCCAAGAACAAGAGGAAGAAGAGTCGAAAAAGGTTGAAATCATCTCCGGCCAGGACTGCTCCGGTGTCGGAGGGATCGCCGGGAAAGTATGCCAGGTCTGCGGCAACTGATCGCTCCACCACCAACAACCGCACTTTCCAAGAAAGTATTGAAGAAGCATGGTTTGATCCGGTTGCAATATTGGAATCTGACTGTGATGAAGATTTCCTAAGCGTACAGGATGATATCTTATCTCTTAATGGCTTTGAACGTACTTCTGTATCAAGTACTTCATCCGCAAAAGATGGCAATTATGGGGACTATATTATTCGCGATCTACATGCCCCTTCAATGGAGCAATTGCATAAAGCAGGGGATTTATCACCAGAAAATTCTGCAAACTCTATTATCGTGGCACAAAAGGGCAATAGACAAGTATTTAATGACGGTGATTCCCAAGCTAAATGTGATGATCATTCAACTACTGAAGCAAATCAACCTGTATTCCTTGATGAAATCTCCTCCTCCGTGGATGAAAGTTCTGCCAAGGAGGATAGTATATTGGATAATTGTGGGATTCTTCCAAGCAACTGTCTACCTTGTCTTGTTTCAACTGTTACCTCTGATGAAAAGAGACGTTCACTAAGCTATAGTCCACCAAGCGCAAGGAAAAAGTCTGCCTTAAAGCTTCCCTTCAAATTGAAAGAACATGCGAATGCAAGTTTATTTTCGTCAAAGGTGATTCTAGAAAGACCAATAGCAGGTTCCCAAGTTCCTTTTTGCCCTATAGAAAAGAACATGTTCGATTCTTGGTCACAAATTGATCCAAGTACTTTCAAAATTCGAGGAGTAAATTATCTTAGGGACAAGAAGAAGGAATTTGCTGCGAACTATGCTGCATATTATCCATTTGGTCTTGATGTATTTTTGTCCCAGCGGAAAATTAATCATATTGCTCAGTTTGTGAAACTCCCTGCTGCTAGTACTTCTGGAAATCTGCCTGCTATTCTTGTTGTAAATGTACAGGTACCATTGTATCCCGCTGCAATTTTTCAGGGCGAGACTGATGGAGAAGGAATGAATTTCGTTTTGTACTTTAAGCTTAATGAGACATACTCAAAAGAACTTCCACCTCACTTTCAAGAAAGTATCAGAAAATTAATTGATGATGAAGTCGAAAAGGTTAAAGGTTTTCCTGTGGATACAATTGCACCCTTTCGAGAAAGATTGAAGATATTGGGACGTGTTGTTAATGTGGAAGATCTTCACTTAAATACAGCAGAGAGGAAGCTTATGCAGGCTTACAATGAAAAACCTGTTCTTTCGCGACCTCAGCATGAGTTCTACTCGGGAGAAAACTACCTTGAGATTGACTTGGATATGCACAGATTCAGTTATATATCTAGGAAAGGGTTTGAAACATTCCTAGATAGACTAAAGATCTGCATCTTGGATGTTGGCCTCACAATTCAGGTTTGCCCTATGATTCCTATCCTGTTTTTCATGCTAGCTTTTGAAAGATGTACATCTATACCTTATATCTGCCTTTCCAAACAGGGGAACAAACCCGAAGAGTTGCCAGAGCAGATCTTATGTTGTATACGGTTGAATGGAATTGACTACATGAACTACAATCAACTGGGGCTGATGCAAGATCCCCTTTTGACCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

568

Amino Acids

63.69

Weight (kDa)

6.22

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 281 - 501 2.5e-45 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 782
Acc65I GGTACC 1 cut(s) 1057
AccB1I GGYRCC 1 cut(s) 1057
AccI GTMKAC 3 cut(s) 17, 625, 1630
AccII CGCG 2 cut(s) 363, 1357
AciI CCGC 5 cut(s) 152, 181, 327, 967, 1071
AclWI GGATC 4 cut(s) 133, 212, 848, 1679
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 4 cut(s) 421, 870, 908, 1111
AcuI CTGAAG 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 9 cut(s) 267, 304, 319, 862, 1015, 1053, 1059, 1124, 1555
AfiI CCNNNNNNNGG 3 cut(s) 382, 791, 1587
AflIII ACRYGT 2 cut(s) 828, 1273
AgeI ACCGGT 1 cut(s) 27
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 2 cut(s) 101, 143
AjuI GAANNNNNNNTTGG 2 cut(s) 778, 810
AloI GAACNNNNNNTCC 2 cut(s) 34, 66
AluBI AGCT 6 cut(s) 491, 678, 718, 1132, 1321, 1541
AluI AGCT 6 cut(s) 491, 678, 718, 1132, 1321, 1541
Alw26I GTCTC 3 cut(s) 658, 1085, 1133
AlwI GGATC 4 cut(s) 133, 212, 848, 1679
Ama87I CYCGRG 1 cut(s) 1378
AoxI GGCC 2 cut(s) 99, 1489
ApeKI GCWGC 4 cut(s) 914, 926, 1007, 1073
ApoI RAATTY 4 cut(s) 421, 870, 908, 1111
AseI ATTAAT 2 cut(s) 975, 1190
AsiGI ACCGGT 1 cut(s) 27
Asp700I GAANNNNTTC 2 cut(s) 830, 1293
Asp718I GGTACC 1 cut(s) 1057
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 3 cut(s) 405, 491, 781
AsuNHI GCTAGC 1 cut(s) 1537
AvaI CYCGRG 1 cut(s) 1378
AvaII GGWCC 1 cut(s) 25
BaeI ACNNNNGTAYC 6 cut(s) 294, 327, 1049, 1049, 1082, 1082
BanI GGYRCC 1 cut(s) 1057
BbvCI CCTCAGC 1 cut(s) 1362
BbvI GCAGC 4 cut(s) 901, 913, 994, 1060
BccI CCATC 2 cut(s) 329, 1091
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 2 cut(s) 103, 145
BciVI GTATCC 2 cut(s) 1077, 1225
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 658, 1085, 1133
BfaI CTAG 5 cut(s) 776, 1011, 1434, 1457, 1538
BfmI CTRYAG 2 cut(s) 679, 816
BfuAI ACCTGC 1 cut(s) 782
BfuI GTATCC 2 cut(s) 1077, 1225
BglII AGATCT 3 cut(s) 1291, 1470, 1617
BisI GCNGC 5 cut(s) 153, 915, 927, 1008, 1074
BlsI GCNGC 5 cut(s) 154, 916, 928, 1009, 1075
BmcAI AGTACT 3 cut(s) 319, 862, 1015
Bme1390I CCNGG 3 cut(s) 103, 132, 145
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 1378
BmgBI CACGTC 1 cut(s) 1274
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 796, 1059
BmrFI CCNGG 3 cut(s) 103, 132, 145
BmrI ACTGGG 1 cut(s) 1679
BmsI GCATC 2 cut(s) 1485, 1668
BmtI GCTAGC 1 cut(s) 1541
BmuI ACTGGG 1 cut(s) 1679
BplI GAGNNNNNCTC 4 cut(s) 1130, 1162, 1361, 1393
Bpu10I CCTNAGC 2 cut(s) 260, 1362
BpuEI CTTGAG 1 cut(s) 1415
BpuMI CCSGG 1 cut(s) 132
BsaBI GATNNNNATC 3 cut(s) 1475, 1520, 1555
BsaHI GRCGYC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 558, 576
BsaWI WCCGGW 3 cut(s) 27, 112, 220
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 791, 1587
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 1674
Bse8I GATNNNNATC 3 cut(s) 1475, 1520, 1555
BseBI CCWGG 2 cut(s) 103, 145
BseDI CCNNGG 2 cut(s) 558, 576
BseGI GGATG 4 cut(s) 277, 323, 568, 1489
BseJI GATNNNNATC 3 cut(s) 1475, 1520, 1555
BseLI CCNNNNNNNGG 3 cut(s) 382, 791, 1587
BseMII CTCAG 3 cut(s) 38, 1001, 1376
BseNI ACTGG 1 cut(s) 1674
BseRI GAGGAG 3 cut(s) 541, 544, 891
BseXI GCAGC 4 cut(s) 901, 913, 994, 1060
BseYI CCCAGC 1 cut(s) 963
Bsh1236I CGCG 2 cut(s) 363, 1357
BshFI GGCC 2 cut(s) 101, 1491
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 1378
BsiSI CCGG 6 cut(s) 23, 28, 98, 113, 131, 221
BslFI GGGAC 4 cut(s) 362, 908, 946, 1284
BslI CCNNNNNNNGG 3 cut(s) 382, 791, 1587
BsmAI GTCTC 3 cut(s) 658, 1085, 1133
BsmBI CGTCTC 1 cut(s) 658
BsmFI GGGAC 4 cut(s) 362, 908, 946, 1284
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 2 cut(s) 101, 1491
BsoBI CYCGRG 1 cut(s) 1378
Bsp1407I TGTACA 2 cut(s) 1051, 1553
Bsp68I TCGCGA 2 cut(s) 363, 1357
BspACI CCGC 5 cut(s) 152, 181, 327, 967, 1071
BspANI GGCC 2 cut(s) 101, 1491
BspCNI CTCAG 3 cut(s) 39, 1000, 1375
BspFNI CGCG 2 cut(s) 363, 1357
BspLI GGNNCC 2 cut(s) 796, 1059
BspMI ACCTGC 1 cut(s) 782
BspOI GCTAGC 1 cut(s) 1541
BspPI GGATC 4 cut(s) 133, 212, 848, 1679
BspT107I GGYRCC 1 cut(s) 1057
BsrFI RCCGGY 1 cut(s) 27
BsrGI TGTACA 2 cut(s) 1051, 1553
BsrI ACTGG 1 cut(s) 1674
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 2 cut(s) 558, 576
BssNAI GTATAC 1 cut(s) 1631
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 576
Bst1107I GTATAC 1 cut(s) 1631
Bst2UI CCWGG 2 cut(s) 103, 145
Bst4CI ACNGT 5 cut(s) 245, 479, 623, 646, 1635
Bst6I CTCTTC 2 cut(s) 65, 1596
BstACI GRCGYC 1 cut(s) 20
BstAUI TGTACA 2 cut(s) 1051, 1553
BstC8I GCNNGC 3 cut(s) 1032, 1329, 1539
BstDEI CTNAG 6 cut(s) 47, 260, 674, 890, 987, 1362
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 277, 323, 568, 1489
BstFNI CGCG 2 cut(s) 363, 1357
BstHHI GCGC 1 cut(s) 695
BstMAI GTCTC 3 cut(s) 658, 1085, 1133
BstMWI GCNNNNNNNGC 4 cut(s) 107, 149, 923, 1363
BstNI CCWGG 2 cut(s) 103, 145
BstNSI RCATGY 3 cut(s) 374, 743, 832
BstSCI CCNGG 3 cut(s) 101, 130, 143
BstSFI CTRYAG 2 cut(s) 679, 816
BstUI CGCG 2 cut(s) 363, 1357
BstV1I GCAGC 4 cut(s) 901, 913, 994, 1060
BstX2I RGATCY 4 cut(s) 1291, 1470, 1617, 1684
BstYI RGATCY 4 cut(s) 1291, 1470, 1617, 1684
BstZ17I GTATAC 1 cut(s) 1631
BsuI GTATCC 2 cut(s) 1077, 1225
BsuRI GGCC 2 cut(s) 101, 1491
BtgI CCRYGG 1 cut(s) 558
BtrI CACGTC 1 cut(s) 1274
BtsCI GGATG 4 cut(s) 277, 323, 568, 1489
BtuMI TCGCGA 2 cut(s) 363, 1357
BveI ACCTGC 1 cut(s) 782
Cac8I GCNNGC 3 cut(s) 1032, 1329, 1539
CfoI GCGC 1 cut(s) 695
Cfr10I RCCGGY 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 25
CpoI CGGWCCG 1 cut(s) 25
CseI GACGC 2 cut(s) 22, 28
Csp6I GTAC 9 cut(s) 266, 303, 318, 861, 1014, 1052, 1058, 1123, 1554
CspAI ACCGGT 1 cut(s) 27
CspI CGGWCCG 1 cut(s) 25
CviAII CATG 7 cut(s) 210, 371, 740, 829, 1367, 1534, 1654
CviQI GTAC 9 cut(s) 266, 303, 318, 861, 1014, 1052, 1058, 1123, 1554
DdeI CTNAG 6 cut(s) 47, 260, 674, 890, 987, 1362
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 65, 1596
EarI CTCTTC 2 cut(s) 65, 1596
Eco130I CCWWGG 1 cut(s) 576
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 1378
EcoRII CCWGG 2 cut(s) 101, 143
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
Esp3I CGTCTC 1 cut(s) 658
FaeI CATG 7 cut(s) 213, 374, 743, 832, 1370, 1537, 1657
FalI AAGNNNNNCTT 5 cut(s) 695, 727, 707, 739, 1674
FaqI GGGAC 4 cut(s) 362, 908, 946, 1284
FatI CATG 7 cut(s) 209, 370, 739, 828, 1366, 1533, 1653
FauI CCCGC 1 cut(s) 1078
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 3 cut(s) 17, 625, 1630
Fnu4HI GCNGC 5 cut(s) 153, 915, 927, 1008, 1074
FokI GGATG 4 cut(s) 284, 310, 575, 1496
Fsp4HI GCNGC 5 cut(s) 153, 915, 927, 1008, 1074
FspBI CTAG 5 cut(s) 776, 1011, 1434, 1457, 1538
GlaI GCGC 1 cut(s) 694
GluI GCNGC 5 cut(s) 153, 915, 927, 1008, 1074
GsaI CCCAGC 1 cut(s) 967
HaeIII GGCC 2 cut(s) 101, 1491
HapII CCGG 6 cut(s) 23, 28, 98, 113, 131, 221
HgaI GACGC 2 cut(s) 22, 28
HhaI GCGC 1 cut(s) 695
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 7 cut(s) 213, 374, 743, 832, 1370, 1537, 1657
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 3 cut(s) 716, 1130, 1319
HinfI GANTC 8 cut(s) 73, 236, 482, 607, 772, 836, 1419, 1516
HpaII CCGG 6 cut(s) 23, 28, 98, 113, 131, 221
HphI GGTGA 3 cut(s) 405, 491, 781
Hpy166II GTNNAC 5 cut(s) 18, 626, 671, 686, 1631
Hpy188I TCNGA 5 cut(s) 38, 121, 241, 655, 1184
Hpy188III TCNNGA 8 cut(s) 362, 776, 947, 1020, 1172, 1250, 1356, 1380
Hpy8I GTNNAC 5 cut(s) 18, 626, 671, 686, 1631
Hpy99I CGWCG 2 cut(s) 22, 38
HpyAV CCTTC 5 cut(s) 33, 387, 733, 898, 1097
HpyCH4III ACNGT 5 cut(s) 245, 479, 623, 646, 1635
HpyCH4IV ACGT 3 cut(s) 301, 667, 1273
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 149, 923, 1363
HpyF3I CTNAG 6 cut(s) 47, 260, 674, 890, 987, 1362
HpySE526I ACGT 3 cut(s) 301, 667, 1273
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 7 cut(s) 213, 374, 743, 832, 1370, 1537, 1657
HspAI GCGC 1 cut(s) 693
KpnI GGTACC 1 cut(s) 1061
Ksp22I TGATCA 1 cut(s) 502
LmnI GCTCC 3 cut(s) 115, 170, 385
Lsp1109I GCAGC 4 cut(s) 901, 913, 994, 1060
LweI GCATC 2 cut(s) 1485, 1668
MaeI CTAG 5 cut(s) 776, 1011, 1434, 1457, 1538
MaeII ACGT 3 cut(s) 301, 667, 1273
MaeIII GTNAC 2 cut(s) 646, 843
MfeI CAATTG 2 cut(s) 389, 1236
MflI RGATCY 4 cut(s) 1291, 1470, 1617, 1684
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 2 cut(s) 16, 99
MroXI GAANNNNTTC 2 cut(s) 830, 1293
MslI CAYNNNNRTG 1 cut(s) 744
MspA1I CMGCKG 2 cut(s) 967, 1073
MspI CCGG 6 cut(s) 23, 28, 98, 113, 131, 221
MspR9I CCNGG 3 cut(s) 103, 132, 145
MunI CAATTG 2 cut(s) 389, 1236
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 103, 145
MvnI CGCG 2 cut(s) 363, 1357
MwoI GCNNNNNNNGC 4 cut(s) 107, 149, 923, 1363
NciI CCSGG 1 cut(s) 132
NheI GCTAGC 1 cut(s) 1537
NlaIII CATG 7 cut(s) 213, 374, 743, 832, 1370, 1537, 1657
NlaIV GGNNCC 2 cut(s) 796, 1059
NmuCI GTSAC 1 cut(s) 843
NruI TCGCGA 2 cut(s) 363, 1357
NspI RCATGY 3 cut(s) 374, 743, 832
PciI ACATGT 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 2 cut(s) 830, 1293
PfeI GAWTC 7 cut(s) 236, 482, 607, 772, 836, 1419, 1516
PinAI ACCGGT 1 cut(s) 27
PkrI GCNGC 5 cut(s) 154, 916, 928, 1009, 1075
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 828
PshBI ATTAAT 2 cut(s) 975, 1190
Psp6I CCWGG 2 cut(s) 101, 143
PspFI CCCAGC 1 cut(s) 963
PspGI CCWGG 2 cut(s) 101, 143
PspN4I GGNNCC 2 cut(s) 796, 1059
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 4 cut(s) 1291, 1470, 1617, 1684
RruI TCGCGA 2 cut(s) 363, 1357
RsaI GTAC 9 cut(s) 267, 304, 319, 862, 1015, 1053, 1059, 1124, 1555
RsaNI GTAC 9 cut(s) 266, 303, 318, 861, 1014, 1052, 1058, 1123, 1554
RseI CAYNNNNRTG 1 cut(s) 744
Rsr2I CGGWCCG 1 cut(s) 25
RsrII CGGWCCG 1 cut(s) 25
SalI GTCGAC 1 cut(s) 16
SatI GCNGC 5 cut(s) 153, 915, 927, 1008, 1074
Sau96I GGNCC 1 cut(s) 25
ScaI AGTACT 3 cut(s) 319, 862, 1015
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 3 cut(s) 103, 132, 145
SfaNI GCATC 2 cut(s) 1485, 1668
SfcI CTRYAG 2 cut(s) 679, 816
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 1 cut(s) 1394
SmoI CTYRAG 1 cut(s) 1394
SsiI CCGC 5 cut(s) 152, 181, 327, 967, 1071
SspI AATATT 1 cut(s) 231
SspMI CTAG 5 cut(s) 776, 1011, 1434, 1457, 1538
StyD4I CCNGG 3 cut(s) 101, 130, 143
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 5 cut(s) 245, 479, 623, 646, 1635
TaiI ACGT 3 cut(s) 304, 670, 1276
TaqI TCGA 6 cut(s) 17, 76, 834, 874, 1206, 1249
TatI WGTACW 6 cut(s) 317, 860, 1013, 1051, 1122, 1553
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 7 cut(s) 236, 482, 607, 772, 836, 1419, 1516
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 4 cut(s) 914, 926, 1007, 1073
Tsp45I GTSAC 1 cut(s) 843
TspGWI ACGGA 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 25
VspI ATTAAT 2 cut(s) 975, 1190
XapI RAATTY 4 cut(s) 421, 870, 908, 1111
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 3 cut(s) 374, 743, 832
XmiI GTMKAC 3 cut(s) 17, 625, 1630
XmnI GAANNNNTTC 2 cut(s) 830, 1293
XspI CTAG 5 cut(s) 776, 1011, 1434, 1457, 1538
ZrmI AGTACT 3 cut(s) 319, 862, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.