MD16G1081800.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008071
Physical Location & Seq
Reverse (-)
5730121 .. 5736022
5902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1081800.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGGGGCGTGCGTGTCAAAGCCGGAGGGGTGCGTCGGAGGGAGATTGAGCTCGTCGAAGAGGAAGAGAACCCGGAAGAGGAGGAGAGACGGGCGGGCTAAACAGACTCCGGGTCGGGCCGGTCGGTTGTCGGAAGGATCGCCGGACAAGTTTGATCGGTCCGCCCCGCCTGATCGCTCCACCTTCAACAACCCCACTTTCCAAGAAGGTAGTGAAGACGCATGGTTTGATCCGGTTGCAAGATTTGAGTCCGACTGCGATGAAGATTTCCATAGTGTTCAGGATGAGGTGTTATCTGTGAATGGATTTGAACGCGTATCCGTATCAAGCAATTTGTCATTGAGAGATGCCAATTGCGGAGAGTATAATATTATTGACCTGCATGCCTCATCGGCTGATCAGATGCATAAGAGAGGGGATTCTGCAAACAACTCTGTCAGTGTAGTTTCTCAGAAGAGCATTAATCATATCATGAGTGGTAATGACGTTGATGGACATTCGACTGCTGAAGCAAACCAACCTGTGTTCCTTGATGAAATCTCCTCTTCTGTGGATGAATCTTCTACCAAGGAGGAAGGAATATTGGACAATTGTGGGATTCTTCCAAGCCATTGTCTGCCTTGTCTTGCTTCAACTGTTCCATCGGTTGAAAAGAGAAGGTCACTGAGTTCTAGCCCTCCAAGTGCAAGGAAAAAGGCTGCCATAAAACTTCCCTTCAAATGGAAAGAAGGACATCCTAACGCAAGTTTATTGTCCTCAAAGATGCTTCTGCAAAGACCAATAGCAGGTTCTCAAGTTCCTTTCTGCCCTATGGAAAAGAAAATGTTTGATTCTTGGTCACATATTGAGCCAAATTCTTTCAAAGTTCGGGGACCGAATTATTTTAAGGACAGAAAGAAGGAACATGCTCCCAGCTATGCTGCATATTATCCATTTGGTCTTGACGTATTTTTATCTCAGCGAAAGATAGATCATATAGCTCGGTTTGTCGAGCTGCCTGTTGTTAGTTCTTCTGGAGATCTTCCCGCTATTCTTGTTGTAAATGTACAGGTACCGTTGTATCCTGCTGCAATTTTTCAGGGAGAGACAGATGGAGAAGGGATGAATTTTGTTTTGTACTTTAAGCTTAATGATATGTACTCTAAAGAACTTCCACCTAATTTTCAAGAAAATATCAGAAGGTTAATTGGCGATGAAGTAGAAAAGGTCAAAGGTTTTCCTGTGGACACAATTGTTCCCTTTCGAGAAAGGTTGAAAATATTGGGACGTGTTGCTAATGTGGAAGATCTTCACTTGAGTGCACCGGAGCGGAAACTTATGCAGGCTTACAATGAAAAGCCCGTTCTTTCACGTCCTCAACATGAATTTTACATGGGAGAAAACTACCTCGAGATTGATTTGGATATGCACAGATTTAGTTACATCTCTAGGAAAGGGTTTGAAGCGTTCCTAGATAGACTAAAGCACTGCATATTGGATGTCGGGCTCACGATTCAGGGGAACAAACCCGAAGAGTTGCCGGAGCAGATCTTATGTTGTATACGATTGAACGGAATTGATTACATGAACTACCACCAACTGGGGTTGACCCAAGATCCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

535

Amino Acids

59.96

Weight (kDa)

6.45

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 279 - 520 5.2e-57 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 387, 776
Acc65I GGTACC 1 cut(s) 1051
AccB1I GGYRCC 1 cut(s) 1051
AccB7I CCANNNNNTGG 1 cut(s) 1579
AccBSI CCGCTC 1 cut(s) 1309
AccI GTMKAC 1 cut(s) 1540
AccII CGCG 1 cut(s) 315
AciI CCGC 6 cut(s) 94, 162, 167, 357, 1026, 1309
AclWI GGATC 3 cut(s) 145, 224, 1589
AcsI RAATTY 3 cut(s) 853, 1105, 1364
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 4 cut(s) 1047, 1053, 1118, 1139
AfiI CCNNNNNNNGG 4 cut(s) 78, 720, 785, 1579
AflIII ACRYGT 2 cut(s) 313, 1267
AhdI GACNNNNNGTC 1 cut(s) 111
AjiI CACGTC 2 cut(s) 1268, 1352
AjuI GAANNNNNNNTTGG 2 cut(s) 772, 804
AleI CACNNNNGTG 1 cut(s) 1296
AluBI AGCT 5 cut(s) 51, 915, 980, 994, 1126
AluI AGCT 5 cut(s) 51, 915, 980, 994, 1126
Alw21I GWGCWC 2 cut(s) 53, 1303
Alw26I GTCTC 2 cut(s) 81, 1079
Alw44I GTGCAC 1 cut(s) 1299
AlwI GGATC 3 cut(s) 145, 224, 1589
Ama87I CYCGRG 1 cut(s) 1388
AoxI GGCC 1 cut(s) 117
ApaLI GTGCAC 1 cut(s) 1299
ApeKI GCWGC 4 cut(s) 698, 920, 994, 1067
ApoI RAATTY 3 cut(s) 853, 1105, 1364
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AseI ATTAAT 1 cut(s) 462
Asp700I GAANNNNTTC 1 cut(s) 1287
Asp718I GGTACC 1 cut(s) 1051
AspS9I GGNCC 3 cut(s) 117, 159, 872
AsuC2I CCSGG 2 cut(s) 73, 111
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 2 cut(s) 159, 872
BaeGI GKGCMC 1 cut(s) 1303
BaeI ACNNNNGTAYC 4 cut(s) 1043, 1043, 1076, 1076
BanI GGYRCC 1 cut(s) 1051
BanII GRGCYC 2 cut(s) 53, 1488
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 53, 1303
BbvI GCAGC 4 cut(s) 685, 907, 981, 1054
BccI CCATC 3 cut(s) 485, 649, 1085
BciVI GTATCC 2 cut(s) 328, 1071
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 2 cut(s) 73, 111
BcoDI GTCTC 2 cut(s) 81, 1079
BfaI CTAG 3 cut(s) 672, 1428, 1451
BfuAI ACCTGC 2 cut(s) 387, 776
BfuI GTATCC 2 cut(s) 328, 1071
BglI GCCNNNNNGGC 1 cut(s) 392
BglII AGATCT 3 cut(s) 1018, 1285, 1527
BisI GCNGC 4 cut(s) 699, 921, 995, 1068
BlsI GCNGC 4 cut(s) 700, 922, 996, 1069
Bme1390I CCNGG 2 cut(s) 73, 111
Bme18I GGWCC 2 cut(s) 159, 872
BmeRI GACNNNNNGTC 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 1388
BmgBI CACGTC 2 cut(s) 1268, 1352
BmgT120I GGNCC 3 cut(s) 117, 159, 872
BmiI GGNNCC 2 cut(s) 873, 1053
BmrFI CCNGG 2 cut(s) 73, 111
BmrI ACTGGG 1 cut(s) 1589
BmsI GCATC 3 cut(s) 337, 393, 753
BmuI ACTGGG 1 cut(s) 1589
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 1035
BpuEI CTTGAG 2 cut(s) 777, 1315
BpuMI CCSGG 2 cut(s) 73, 111
BsaJI CCNNGG 1 cut(s) 567
BsaWI WCCGGW 2 cut(s) 232, 1303
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 720, 785, 1579
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 1584
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 5 cut(s) 289, 559, 733, 1107, 1483
BseLI CCNNNNNNNGG 4 cut(s) 78, 720, 785, 1579
BseMII CTCAG 3 cut(s) 464, 656, 971
BseNI ACTGG 1 cut(s) 1584
BseRI GAGGAG 3 cut(s) 94, 97, 532
BseSI GKGCMC 1 cut(s) 1303
BseXI GCAGC 4 cut(s) 685, 907, 981, 1054
BseYI CCCAGC 1 cut(s) 911
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 1051
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 53, 1303
BsiHKCI CYCGRG 1 cut(s) 1388
BsiSI CCGG 8 cut(s) 23, 73, 110, 120, 143, 233, 1304, 1520
BslFI GGGAC 2 cut(s) 885, 1278
BslI CCNNNNNNNGG 4 cut(s) 78, 720, 785, 1579
BsmAI GTCTC 2 cut(s) 81, 1079
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 2 cut(s) 885, 1278
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 1388
Bsp1286I GDGCHC 3 cut(s) 53, 1303, 1488
Bsp1407I TGTACA 1 cut(s) 1045
BspACI CCGC 6 cut(s) 94, 162, 167, 357, 1026, 1309
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 3 cut(s) 463, 657, 970
BspFNI CGCG 1 cut(s) 315
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 873, 1053
BspMI ACCTGC 2 cut(s) 387, 776
BspPI GGATC 3 cut(s) 145, 224, 1589
BspQI GCTCTTC 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 1051
BsrBI CCGCTC 1 cut(s) 1309
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 1045
BsrI ACTGG 1 cut(s) 1584
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 567
BssNAI GTATAC 1 cut(s) 1541
BssT1I CCWWGG 1 cut(s) 567
Bst1107I GTATAC 1 cut(s) 1541
Bst4CI ACNGT 2 cut(s) 637, 1056
Bst6I CTCTTC 6 cut(s) 53, 59, 71, 449, 550, 1506
BstAUI TGTACA 1 cut(s) 1045
BstC8I GCNNGC 4 cut(s) 10, 96, 384, 1323
BstDEI CTNAG 3 cut(s) 450, 665, 957
BstF5I GGATG 5 cut(s) 289, 559, 733, 1107, 1483
BstFNI CGCG 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 81, 1079
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNSI RCATGY 2 cut(s) 386, 908
BstSCI CCNGG 2 cut(s) 71, 109
BstSLI GKGCMC 1 cut(s) 1303
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 4 cut(s) 685, 907, 981, 1054
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 4 cut(s) 1018, 1285, 1527, 1594
BstYI RGATCY 4 cut(s) 1018, 1285, 1527, 1594
BstZ17I GTATAC 1 cut(s) 1541
BsuI GTATCC 2 cut(s) 328, 1071
BsuRI GGCC 1 cut(s) 119
BtgZI GCGATG 2 cut(s) 273, 1206
BtrI CACGTC 2 cut(s) 1268, 1352
BtsCI GGATG 5 cut(s) 289, 559, 733, 1107, 1483
BtsI GCAGTG 1 cut(s) 1465
BtsIMutI CAGTG 3 cut(s) 445, 662, 1465
BveI ACCTGC 2 cut(s) 387, 776
Cac8I GCNNGC 4 cut(s) 10, 96, 384, 1323
CciI TCATGA 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 117, 159, 872
CpoI CGGWCCG 1 cut(s) 159
CseI GACGC 2 cut(s) 22, 227
Csp6I GTAC 4 cut(s) 1046, 1052, 1117, 1138
CspI CGGWCCG 1 cut(s) 159
CviAII CATG 7 cut(s) 222, 383, 472, 905, 1361, 1372, 1564
CviQI GTAC 4 cut(s) 1046, 1052, 1117, 1138
DdeI CTNAG 3 cut(s) 450, 665, 957
DriI GACNNNNNGTC 1 cut(s) 111
Eam1104I CTCTTC 6 cut(s) 53, 59, 71, 449, 550, 1506
Eam1105I GACNNNNNGTC 1 cut(s) 111
EarI CTCTTC 6 cut(s) 53, 59, 71, 449, 550, 1506
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 567
Eco24I GRGCYC 2 cut(s) 53, 1488
Eco47I GGWCC 2 cut(s) 159, 872
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 1388
EcoICRI GAGCTC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 53, 1488
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 7 cut(s) 225, 386, 475, 908, 1364, 1375, 1567
FalI AAGNNNNNCTT 1 cut(s) 1584
FaqI GGGAC 2 cut(s) 885, 1278
FatI CATG 7 cut(s) 221, 382, 471, 904, 1360, 1371, 1563
FauI CCCGC 3 cut(s) 87, 174, 1033
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 1540
Fnu4HI GCNGC 4 cut(s) 699, 921, 995, 1068
FokI GGATG 5 cut(s) 296, 566, 720, 1114, 1490
FriOI GRGCYC 2 cut(s) 53, 1488
Fsp4HI GCNGC 4 cut(s) 699, 921, 995, 1068
FspBI CTAG 3 cut(s) 672, 1428, 1451
GluI GCNGC 4 cut(s) 699, 921, 995, 1068
GsaI CCCAGC 1 cut(s) 915
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 1 cut(s) 119
HapII CCGG 8 cut(s) 23, 73, 110, 120, 143, 233, 1304, 1520
HgaI GACGC 2 cut(s) 22, 227
Hin1II CATG 7 cut(s) 225, 386, 475, 908, 1364, 1375, 1567
HincII GTYRAC 1 cut(s) 1587
HindII GTYRAC 1 cut(s) 1587
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 7 cut(s) 106, 248, 419, 557, 598, 830, 1492
HpaII CCGG 8 cut(s) 23, 73, 110, 120, 143, 233, 1304, 1520
Hpy166II GTNNAC 4 cut(s) 1225, 1301, 1541, 1587
Hpy188I TCNGA 6 cut(s) 38, 133, 253, 402, 453, 1178
Hpy188III TCNNGA 8 cut(s) 281, 472, 941, 1014, 1166, 1244, 1390, 1489
Hpy8I GTNNAC 4 cut(s) 1225, 1301, 1541, 1587
Hpy99I CGWCG 2 cut(s) 38, 58
HpyCH4III ACNGT 2 cut(s) 637, 1056
HpyCH4IV ACGT 4 cut(s) 486, 945, 1267, 1351
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 3 cut(s) 450, 665, 957
HpySE526I ACGT 4 cut(s) 486, 945, 1267, 1351
Hsp92II CATG 7 cut(s) 225, 386, 475, 908, 1364, 1375, 1567
KpnI GGTACC 1 cut(s) 1055
Ksp22I TGATCA 1 cut(s) 397
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 4 cut(s) 182, 913, 1306, 1522
Lsp1109I GCAGC 4 cut(s) 685, 907, 981, 1054
LweI GCATC 3 cut(s) 337, 393, 753
MaeI CTAG 3 cut(s) 672, 1428, 1451
MaeII ACGT 4 cut(s) 486, 945, 1267, 1351
MaeIII GTNAC 3 cut(s) 660, 837, 1418
MbiI CCGCTC 1 cut(s) 1309
MfeI CAATTG 3 cut(s) 352, 589, 1230
MflI RGATCY 4 cut(s) 1018, 1285, 1527, 1594
MhlI GDGCHC 3 cut(s) 53, 1303, 1488
MluI ACGCGT 1 cut(s) 313
MlyI GAGTC 2 cut(s) 100, 257
MmeI TCCRAC 3 cut(s) 16, 111, 276
Mph1103I ATGCAT 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 1287
MseI TTAA 6 cut(s) 462, 885, 1122, 1128, 1184, 1603
MslI CAYNNNNRTG 1 cut(s) 1296
MspI CCGG 8 cut(s) 23, 73, 110, 120, 143, 233, 1304, 1520
MspR9I CCNGG 2 cut(s) 73, 111
MunI CAATTG 3 cut(s) 352, 589, 1230
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 392
NciI CCSGG 2 cut(s) 73, 111
NlaIII CATG 7 cut(s) 225, 386, 475, 908, 1364, 1375, 1567
NlaIV GGNNCC 2 cut(s) 873, 1053
NmuCI GTSAC 2 cut(s) 660, 837
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 2 cut(s) 386, 908
OliI CACNNNNGTG 1 cut(s) 1296
PaeI GCATGC 1 cut(s) 386
PaeR7I CTCGAG 1 cut(s) 1388
PagI TCATGA 1 cut(s) 471
PciSI GCTCTTC 1 cut(s) 449
PdmI GAANNNNTTC 1 cut(s) 1287
PfeI GAWTC 5 cut(s) 419, 557, 598, 830, 1492
PflMI CCANNNNNTGG 1 cut(s) 1579
PkrI GCNGC 4 cut(s) 700, 922, 996, 1069
PleI GAGTC 2 cut(s) 100, 256
PpsI GAGTC 2 cut(s) 100, 256
PshBI ATTAAT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 911
PspN4I GGNNCC 2 cut(s) 873, 1053
PspPI GGNCC 3 cut(s) 117, 159, 872
PsuI RGATCY 4 cut(s) 1018, 1285, 1527, 1594
RsaI GTAC 4 cut(s) 1047, 1053, 1118, 1139
RsaNI GTAC 4 cut(s) 1046, 1052, 1117, 1138
RseI CAYNNNNRTG 1 cut(s) 1296
Rsr2I CGGWCCG 1 cut(s) 159
RsrII CGGWCCG 1 cut(s) 159
SacI GAGCTC 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 449
SaqAI TTAA 6 cut(s) 462, 885, 1122, 1128, 1184, 1603
SatI GCNGC 4 cut(s) 699, 921, 995, 1068
Sau96I GGNCC 3 cut(s) 117, 159, 872
SchI GAGTC 2 cut(s) 100, 257
ScrFI CCNGG 2 cut(s) 73, 111
SduI GDGCHC 3 cut(s) 53, 1303, 1488
SfaNI GCATC 3 cut(s) 337, 393, 753
Sfr274I CTCGAG 1 cut(s) 1388
SinI GGWCC 2 cut(s) 159, 872
SlaI CTCGAG 1 cut(s) 1388
SmiMI CAYNNNNRTG 1 cut(s) 1296
SmlI CTYRAG 3 cut(s) 792, 1294, 1388
SmoI CTYRAG 3 cut(s) 792, 1294, 1388
SphI GCATGC 1 cut(s) 386
SsiI CCGC 6 cut(s) 94, 162, 167, 357, 1026, 1309
SspI AATATT 3 cut(s) 370, 582, 1260
SspMI CTAG 3 cut(s) 672, 1428, 1451
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 2 cut(s) 71, 109
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 637, 1056
TaiI ACGT 4 cut(s) 489, 948, 1270, 1354
TaqI TCGA 5 cut(s) 56, 500, 990, 1243, 1389
TaqII GACCGA 2 cut(s) 147, 889
TatI WGTACW 3 cut(s) 1045, 1116, 1137
TfiI GAWTC 5 cut(s) 419, 557, 598, 830, 1492
Tru1I TTAA 6 cut(s) 462, 885, 1122, 1128, 1184, 1603
Tru9I TTAA 6 cut(s) 462, 885, 1122, 1128, 1184, 1603
TscAI CASTG 3 cut(s) 445, 669, 1472
TseFI GTSAC 2 cut(s) 660, 837
TseI GCWGC 4 cut(s) 698, 920, 994, 1067
Tsp45I GTSAC 2 cut(s) 660, 837
TspDTI ATGAA 8 cut(s) 276, 549, 570, 1118, 1209, 1347, 1377, 1580
TspGWI ACGGA 2 cut(s) 310, 1566
TspRI CASTG 3 cut(s) 445, 669, 1472
Van91I CCANNNNNTGG 1 cut(s) 1579
VneI GTGCAC 1 cut(s) 1299
VpaK11BI GGWCC 2 cut(s) 159, 872
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 3 cut(s) 853, 1105, 1364
XceI RCATGY 2 cut(s) 386, 908
XhoI CTCGAG 1 cut(s) 1388
XmiI GTMKAC 1 cut(s) 1540
XmnI GAANNNNTTC 1 cut(s) 1287
XspI CTAG 3 cut(s) 672, 1428, 1451
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.