Rh4AG420000

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008071
Physical Location & Seq
Forward (+)
71739674 .. 71744315
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG420000.1

Sequence Viewer

Length: 1566 bp
ATGTGGGAACTCATTTACTCTGACAAACTAGGCAGGCTCCGGTGGAATTTTCTTTTCAAGCATTCTATTTCTCAAATAACATGTTACCGTAAACTTCTTTATGCCATTGCCTCTTGTAACTGTTATAACTGTTATGCATCCACAGAAAGTACTGAAGAAGCATGGTTTGATCCGGTTGCAATATTGGAATCTGACTGTGATGAAGATTACCTAAGTGTTCATGATGAGATTTCATCTCTAAATGGTTTTGAACATGCTTCTGTATCAAGTACTTCATCTGCAAAAGATGCCAATTATGGGGACTATATTATTAGCGATGTCCATGGCCCTACAGTGGAGCAGATGCATAAAGCAGGGGATGTATCACCAGAAAATTCTGCAAACTCTGTCACGGTGGTTGTGCAAAAGGACGACAGACAAGTATTTAATGACGGTGATTCCCAAGCTAAATGTCATGGTCATTCAACAACCGAAGCGAATCAACCTGTATTCCTCGATGAAATCTCCTCCTCTGTGGATGAAAGCTCTTCCAAGGAGGAAGGTATGTTGGATAATTGTGGGATTCTTCCAATCAACTGTCTGCCTTGTCTTGTTTCAACTGAATCCTCGGATGGAAAGAGACGCTCACTAAGTTATAGTCCACCAAGTGCAAGGAAAAGGGCTGCCATAAAACTTCCCTTCAAATGGAAAGAACCTGCCAATGCAAGTTTATTTTCATCAAAGATGATTCTAGAAAGACCAATAGCAGGTTCCCAAGTTCCTTTTTGCCCTATAGAAAAGAACATGTTCGATTCTTGGTCACAAATTGAGCCACGTACTTTCAAAATTCGGGGAGTGAACTATCTTAGGGACAAGAAGAAGGAATTTGCTGCGAACTATGCTGCATATTATCCATTTGGTCTTGACGTATTTTTATCTCAGCGAAAAGTAAATCATATAGCTCGGTTTGTGAAACTCCCTGCTGCTAGTTCTTCTGGAAATCTGCCTGCTATTCTTGTTGTAAATGTACAGGTACCATTGTATCCCGCTGCAATTTTTCAGGGTGAGACTGATGGAGAAGGAATGAATTTTGTTTTGTACTTTAAGCTTAATGAGTCATACTCAAAAGAACTTCCACCTCACTTTCAAGAAAGTATCAGAAAATTAATTGATGATGAAGTTGAAAAGGTTAAAGGTTTTCCTGTGGATACAATTGCACCCTTTCGAGAAAGGTTGAAAATATTGGGACGTGTTGTTAATGTGGAAGATCTTCATTTAAATACAGCAGAGCGGAAGCTTATGCAGGCTTACAATGAAAAACCTGTTCTTTCGCGACCTCAGCATGAGTTCTACTTGGGAGAAAACTACCTTGAGATTGACTTGGATATGCACAGATTTAGTTATATATCTAGGAAAGGGTTTGAAACATTCCTAGATAGACTGAAGATCTGCATCTTGGATGTCGGCCTCACAATTCAGGGGAACAAACCCGAAGAGTTGCCAGAGCAGATCTTATGTTGTATACGGTTGAATGGAATTGACTACATGAACTACAATCAACTGGGGTTGACCCAAGATCCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

521

Amino Acids

59.0

Weight (kDa)

5.39

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 266 - 507 6.8e-57 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 126
Acc36I ACCTGC 2 cut(s) 703, 737
Acc65I GGTACC 1 cut(s) 1012
AccB1I GGYRCC 1 cut(s) 1012
AccBSI CCGCTC 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 1501
AccII CGCG 1 cut(s) 1312
AciI CCGC 2 cut(s) 1026, 1270
AclWI GGATC 2 cut(s) 164, 1550
AcsI RAATTY 5 cut(s) 46, 373, 825, 863, 1066
AcuI CTGAAG 2 cut(s) 174, 1442
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 6 cut(s) 151, 271, 817, 1008, 1014, 1079
AfiI CCNNNNNNNGG 4 cut(s) 297, 334, 684, 746
AflIII ACRYGT 3 cut(s) 80, 783, 1228
AjiI CACGTC 1 cut(s) 1229
AjuI GAANNNNNNNTTGG 2 cut(s) 733, 765
AluBI AGCT 5 cut(s) 446, 525, 941, 1087, 1276
AluI AGCT 5 cut(s) 446, 525, 941, 1087, 1276
Alw26I GTCTC 2 cut(s) 613, 1040
AlwI GGATC 2 cut(s) 164, 1550
AoxI GGCC 2 cut(s) 325, 1444
ApeKI GCWGC 5 cut(s) 662, 869, 881, 962, 1028
ApoI RAATTY 5 cut(s) 46, 373, 825, 863, 1066
AseI ATTAAT 1 cut(s) 1145
Asp700I GAANNNNTTC 2 cut(s) 785, 1248
Asp718I GGTACC 1 cut(s) 1012
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 3 cut(s) 357, 446, 1055
BaeI ACNNNNGTAYC 4 cut(s) 1004, 1004, 1037, 1037
BanI GGYRCC 1 cut(s) 1012
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 5 cut(s) 649, 856, 868, 949, 1015
BccI CCATC 2 cut(s) 605, 1046
BciVI GTATCC 2 cut(s) 1032, 1180
BcoDI GTCTC 2 cut(s) 613, 1040
BfaI CTAG 5 cut(s) 29, 731, 966, 1389, 1412
BfmI CTRYAG 2 cut(s) 330, 771
BfuAI ACCTGC 2 cut(s) 703, 737
BfuI GTATCC 2 cut(s) 1032, 1180
BglII AGATCT 3 cut(s) 1246, 1425, 1488
BisI GCNGC 5 cut(s) 663, 870, 882, 963, 1029
BlsI GCNGC 5 cut(s) 664, 871, 883, 964, 1030
BmcAI AGTACT 2 cut(s) 151, 271
BmgBI CACGTC 1 cut(s) 1229
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 38, 751, 1014
BmrI ACTGGG 1 cut(s) 1550
BmsI GCATC 4 cut(s) 146, 277, 333, 1440
BmuI ACTGGG 1 cut(s) 1550
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
Bpu10I CCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 1 cut(s) 1370
BsaAI YACGTR 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 1430
BsaJI CCNNGG 3 cut(s) 322, 531, 606
BsaWI WCCGGW 2 cut(s) 39, 172
Bsc4I CCNNNNNNNGG 4 cut(s) 297, 334, 684, 746
Bse1I ACTGG 1 cut(s) 1545
Bse3DI GCAATG 1 cut(s) 105
Bse8I GATNNNNATC 1 cut(s) 1430
BseDI CCNNGG 3 cut(s) 322, 531, 606
BseGI GGATG 5 cut(s) 137, 364, 523, 616, 1444
BseJI GATNNNNATC 1 cut(s) 1430
BseLI CCNNNNNNNGG 4 cut(s) 297, 334, 684, 746
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 932, 1331
BseNI ACTGG 1 cut(s) 1545
BseRI GAGGAG 2 cut(s) 496, 499
BseXI GCAGC 5 cut(s) 649, 856, 868, 949, 1015
Bsh1236I CGCG 1 cut(s) 1312
BshFI GGCC 2 cut(s) 327, 1446
BshNI GGYRCC 1 cut(s) 1012
BsiSI CCGG 2 cut(s) 40, 173
BslFI GGGAC 3 cut(s) 314, 863, 1239
BslI CCNNNNNNNGG 4 cut(s) 297, 334, 684, 746
BsmAI GTCTC 2 cut(s) 613, 1040
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 3 cut(s) 314, 863, 1239
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 2 cut(s) 327, 1446
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
Bsp19I CCATGG 1 cut(s) 322
Bsp68I TCGCGA 1 cut(s) 1312
BspACI CCGC 2 cut(s) 1026, 1270
BspANI GGCC 2 cut(s) 327, 1446
BspCNI CTCAG 2 cut(s) 931, 1330
BspFNI CGCG 1 cut(s) 1312
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 38, 751, 1014
BspMI ACCTGC 2 cut(s) 703, 737
BspPI GGATC 2 cut(s) 164, 1550
BspQI GCTCTTC 1 cut(s) 532
BspT107I GGYRCC 1 cut(s) 1012
BsrBI CCGCTC 1 cut(s) 1270
BsrDI GCAATG 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 1 cut(s) 1545
BssECI CCNNGG 3 cut(s) 322, 531, 606
BssMI GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
BssNAI GTATAC 1 cut(s) 1502
BssT1I CCWWGG 2 cut(s) 322, 531
Bst1107I GTATAC 1 cut(s) 1502
Bst4CI ACNGT 9 cut(s) 89, 122, 131, 197, 334, 394, 434, 578, 1506
Bst6I CTCTTC 2 cut(s) 532, 1467
BstAPI GCANNNNNTGC 1 cut(s) 287
BstAUI TGTACA 1 cut(s) 1006
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 35, 987, 1284
BstDEI CTNAG 5 cut(s) 212, 629, 845, 918, 1317
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 5 cut(s) 137, 364, 523, 616, 1444
BstFNI CGCG 1 cut(s) 1312
BstKTI GATC 5 cut(s) 172, 1249, 1428, 1491, 1558
BstMAI GTCTC 2 cut(s) 613, 1040
BstMBI GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
BstMWI GCNNNNNNNGC 3 cut(s) 287, 878, 1318
BstNSI RCATGY 3 cut(s) 84, 257, 787
BstSFI CTRYAG 2 cut(s) 330, 771
BstUI CGCG 1 cut(s) 1312
BstV1I GCAGC 5 cut(s) 649, 856, 868, 949, 1015
BstX2I RGATCY 4 cut(s) 1246, 1425, 1488, 1555
BstYI RGATCY 4 cut(s) 1246, 1425, 1488, 1555
BstZ17I GTATAC 1 cut(s) 1502
BsuI GTATCC 2 cut(s) 1032, 1180
BsuRI GGCC 2 cut(s) 327, 1446
BtgI CCRYGG 1 cut(s) 322
BtgZI GCGATG 1 cut(s) 330
BtrI CACGTC 1 cut(s) 1229
BtsCI GGATG 5 cut(s) 137, 364, 523, 616, 1444
BtsIMutI CAGTG 1 cut(s) 339
BtuMI TCGCGA 1 cut(s) 1312
BveI ACCTGC 2 cut(s) 703, 737
Cac8I GCNNGC 3 cut(s) 35, 987, 1284
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 1 cut(s) 630
Csp6I GTAC 6 cut(s) 150, 270, 816, 1007, 1013, 1078
CviAII CATG 9 cut(s) 81, 162, 221, 254, 323, 455, 784, 1322, 1525
CviQI GTAC 6 cut(s) 150, 270, 816, 1007, 1013, 1078
DdeI CTNAG 5 cut(s) 212, 629, 845, 918, 1317
DpnI GATC 5 cut(s) 171, 1248, 1427, 1490, 1557
DpnII GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
DraI TTTAAA 1 cut(s) 1257
DraIII CACNNNGTG 1 cut(s) 647
Eam1104I CTCTTC 2 cut(s) 532, 1467
EarI CTCTTC 2 cut(s) 532, 1467
Eco130I CCWWGG 2 cut(s) 322, 531
Eco57I CTGAAG 2 cut(s) 174, 1442
EcoT14I CCWWGG 2 cut(s) 322, 531
EcoT22I ATGCAT 2 cut(s) 139, 348
ErhI CCWWGG 2 cut(s) 322, 531
Esp3I CGTCTC 1 cut(s) 613
FaeI CATG 9 cut(s) 84, 165, 224, 257, 326, 458, 787, 1325, 1528
FalI AAGNNNNNCTT 1 cut(s) 1545
FaqI GGGAC 3 cut(s) 314, 863, 1239
FatI CATG 9 cut(s) 80, 161, 220, 253, 322, 454, 783, 1321, 1524
FauI CCCGC 1 cut(s) 1033
FblI GTMKAC 1 cut(s) 1501
Fnu4HI GCNGC 5 cut(s) 663, 870, 882, 963, 1029
FokI GGATG 5 cut(s) 124, 371, 530, 623, 1451
Fsp4HI GCNGC 5 cut(s) 663, 870, 882, 963, 1029
FspBI CTAG 5 cut(s) 29, 731, 966, 1389, 1412
GluI GCNGC 5 cut(s) 663, 870, 882, 963, 1029
HaeIII GGCC 2 cut(s) 327, 1446
HapII CCGG 2 cut(s) 40, 173
HgaI GACGC 1 cut(s) 630
Hin1II CATG 9 cut(s) 84, 165, 224, 257, 326, 458, 787, 1325, 1528
HincII GTYRAC 1 cut(s) 1548
HindII GTYRAC 1 cut(s) 1548
HindIII AAGCTT 2 cut(s) 1085, 1274
HinfI GANTC 8 cut(s) 188, 437, 478, 562, 602, 727, 791, 1094
HpaII CCGG 2 cut(s) 40, 173
HphI GGTGA 3 cut(s) 357, 446, 1055
Hpy166II GTNNAC 5 cut(s) 92, 641, 838, 1502, 1548
Hpy188I TCNGA 4 cut(s) 22, 193, 610, 1139
Hpy188III TCNNGA 7 cut(s) 221, 731, 902, 975, 1127, 1205, 1311
Hpy8I GTNNAC 5 cut(s) 92, 641, 838, 1502, 1548
HpyAV CCTTC 4 cut(s) 533, 688, 853, 1052
HpyCH4III ACNGT 9 cut(s) 89, 122, 131, 197, 334, 394, 434, 578, 1506
HpyCH4IV ACGT 3 cut(s) 814, 906, 1228
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 878, 1318
HpyF3I CTNAG 5 cut(s) 212, 629, 845, 918, 1317
HpySE526I ACGT 3 cut(s) 814, 906, 1228
Hsp92II CATG 9 cut(s) 84, 165, 224, 257, 326, 458, 787, 1325, 1528
KpnI GGTACC 1 cut(s) 1016
Kzo9I GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
LguI GCTCTTC 1 cut(s) 532
LmnI GCTCC 2 cut(s) 42, 337
Lsp1109I GCAGC 5 cut(s) 649, 856, 868, 949, 1015
LweI GCATC 4 cut(s) 146, 277, 333, 1440
MaeI CTAG 5 cut(s) 29, 731, 966, 1389, 1412
MaeII ACGT 3 cut(s) 814, 906, 1228
MaeIII GTNAC 4 cut(s) 83, 116, 388, 798
MalI GATC 5 cut(s) 171, 1248, 1427, 1490, 1557
MbiI CCGCTC 1 cut(s) 1270
MboI GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 4 cut(s) 1246, 1425, 1488, 1555
MlyI GAGTC 1 cut(s) 1103
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 9 cut(s) 121, 503, 517, 520, 529, 616, 1128, 1326, 1457
Mph1103I ATGCAT 2 cut(s) 139, 348
MroXI GAANNNNTTC 2 cut(s) 785, 1248
MseI TTAA 7 cut(s) 426, 1083, 1089, 1145, 1170, 1236, 1256
MspA1I CMGCKG 1 cut(s) 1028
MspI CCGG 2 cut(s) 40, 173
MunI CAATTG 1 cut(s) 1191
Mva1269I GAATGC 1 cut(s) 61
MvnI CGCG 1 cut(s) 1312
MwoI GCNNNNNNNGC 3 cut(s) 287, 878, 1318
NcoI CCATGG 1 cut(s) 322
NdeII GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
NlaIII CATG 9 cut(s) 84, 165, 224, 257, 326, 458, 787, 1325, 1528
NlaIV GGNNCC 3 cut(s) 38, 751, 1014
NmuCI GTSAC 2 cut(s) 388, 798
NruI TCGCGA 1 cut(s) 1312
NsiI ATGCAT 2 cut(s) 139, 348
NspI RCATGY 3 cut(s) 84, 257, 787
PagI TCATGA 1 cut(s) 220
PciI ACATGT 2 cut(s) 80, 783
PciSI GCTCTTC 1 cut(s) 532
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 2 cut(s) 785, 1248
PfeI GAWTC 7 cut(s) 188, 437, 478, 562, 602, 727, 791
PkrI GCNGC 5 cut(s) 664, 871, 883, 964, 1030
PleI GAGTC 1 cut(s) 1102
PpsI GAGTC 1 cut(s) 1102
Ppu21I YACGTR 1 cut(s) 815
PscI ACATGT 2 cut(s) 80, 783
PshBI ATTAAT 1 cut(s) 1145
PsiI TTATAA 1 cut(s) 126
PspN4I GGNNCC 3 cut(s) 38, 751, 1014
PspPI GGNCC 1 cut(s) 326
PsuI RGATCY 4 cut(s) 1246, 1425, 1488, 1555
RruI TCGCGA 1 cut(s) 1312
RsaI GTAC 6 cut(s) 151, 271, 817, 1008, 1014, 1079
RsaNI GTAC 6 cut(s) 150, 270, 816, 1007, 1013, 1078
SapI GCTCTTC 1 cut(s) 532
SaqAI TTAA 7 cut(s) 426, 1083, 1089, 1145, 1170, 1236, 1256
SatI GCNGC 5 cut(s) 663, 870, 882, 963, 1029
Sau3AI GATC 5 cut(s) 169, 1246, 1425, 1488, 1555
Sau96I GGNCC 1 cut(s) 326
ScaI AGTACT 2 cut(s) 151, 271
SchI GAGTC 1 cut(s) 1103
SfaNI GCATC 4 cut(s) 146, 277, 333, 1440
SfcI CTRYAG 2 cut(s) 330, 771
SmiI ATTTAAAT 1 cut(s) 1257
SmlI CTYRAG 1 cut(s) 1349
SmoI CTYRAG 1 cut(s) 1349
SsiI CCGC 2 cut(s) 1026, 1270
SspI AATATT 2 cut(s) 183, 1221
SspMI CTAG 5 cut(s) 29, 731, 966, 1389, 1412
StyI CCWWGG 2 cut(s) 322, 531
SwaI ATTTAAAT 1 cut(s) 1257
TaaI ACNGT 9 cut(s) 89, 122, 131, 197, 334, 394, 434, 578, 1506
TaiI ACGT 3 cut(s) 817, 909, 1231
TaqI TCGA 3 cut(s) 495, 789, 1204
TatI WGTACW 4 cut(s) 149, 269, 1006, 1077
TfiI GAWTC 7 cut(s) 188, 437, 478, 562, 602, 727, 791
Tru1I TTAA 7 cut(s) 426, 1083, 1089, 1145, 1170, 1236, 1256
Tru9I TTAA 7 cut(s) 426, 1083, 1089, 1145, 1170, 1236, 1256
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 388, 798
TseI GCWGC 5 cut(s) 662, 869, 881, 962, 1028
Tsp45I GTSAC 2 cut(s) 388, 798
TspRI CASTG 1 cut(s) 339
VspI ATTAAT 1 cut(s) 1145
XapI RAATTY 5 cut(s) 46, 373, 825, 863, 1066
XbaI TCTAGA 1 cut(s) 730
XceI RCATGY 3 cut(s) 84, 257, 787
XmiI GTMKAC 1 cut(s) 1501
XmnI GAANNNNTTC 2 cut(s) 785, 1248
XspI CTAG 5 cut(s) 29, 731, 966, 1389, 1412
ZrmI AGTACT 2 cut(s) 151, 271
Zsp2I ATGCAT 2 cut(s) 139, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.