FvH4_4g36770

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018707
Physical Location & Seq
Reverse (-)
33538392 .. 33545468
7077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36770.t1

Sequence Viewer

Length: 1134 bp
ATGATTGCAGCAGAAAGAGAGATGGAACCAAAAATGAGCAACAAGGCAACCACAGTGCTGGAAGCACATCCGATGAACGGTGGAGATGGCACATACAGCTACACCAAAAATTCCTATTACCAAAGAGAAGCTGCAAATGTTGCGCAAACTCTTATTGGTGATGTAATAGCCATGAAGCTTGACATAAACGAGTTTGCTTCTTCTTCTACCAATGCATTTTGTATAGCAGATCTGGGGTGTTCAGTTGGACCAAACACCTTTGTTTCTGTGGAGAACATATTGGAAGCAATTAAGCATAAGTACCGGTCCGAATGCATGTCTTCCCAGACGCGTGAATTCCAAGTCTTCTTTAATGATCACGCAGGCAATGATTTCAATACCCTATTTGCAAAATTCCCTCCGACAAGGAACTACTTTGCAGCTGGTGTGCCGGGGTCTTTCCATGGCCGATTATTCCCCAAGTCCTCTCTCCATGTTGTGCATTCTTCATATGCAACCCACTGGCTCTCAAAGGTACCCGAAGAACTGACAGACAAGAACTCTCCGGCATGGAACAAAGGGAAGATTCACTACACAACTGCCCCTGAAGAAGTTGTTAATGCTTATGCAGCTCAATTTACCAAGGACATGGCAGCATTCTTGGAAGCTCGCGCTCAAGAGCTTGTGGTTGGTGGAATGATGATACTAATCTTGCCAGCTGTCCCAACTGGGATCCCTCATTCCCGTGTTCCCAACGGAGTAATGTTCGATTTTATGGGGTCTATTCTCATCGATATGGCAAAGGAAGGAGTAATATCTGAAGCTGAGGTGGACTCATTCAACTTGCCAGTGTACACTACCTCTCCCAAGGAGATGGGAGAGCTGGTAGAGAGAAATGGACGCTTTCACATCGAGCAAATGGAATCAACGAGCCCCTGGCTTGAAATCAAGCATTCCAACTCTGACTACGGGAAGACACTGTCGATGTCGCTAAGAGCTGGCATGGAGGGAGTTCTCAGAAGCCATTTCGGAAGTGAGATTACCAATCAGCTATTTAATAGGCTCTGTGACCAAAGTGGACAACTTACCCGCCAGCTACAGGAGTCCTGCTGCAGAGAAGGTACTCAGTTGTTTGTTGCTCTCAAGCGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

378

Amino Acids

41.89

Weight (kDa)

5.86

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 67 - 376 5e-107 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 514
AccII CGCG 2 cut(s) 331, 651
AciI CCGC 1 cut(s) 1069
AclWI GGATC 2 cut(s) 706, 719
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 3 cut(s) 109, 335, 392
AcuI CTGAAG 2 cut(s) 606, 819
AfaI GTAC 4 cut(s) 302, 516, 833, 1102
AfiI CCNNNNNNNGG 2 cut(s) 77, 1078
AflIII ACRYGT 1 cut(s) 329
AgeI ACCGGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 376, 820, 923
AjnI CCWGG 1 cut(s) 914
AlwI GGATC 2 cut(s) 706, 719
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 6 cut(s) 8, 131, 419, 608, 632, 1089
ApoI RAATTY 3 cut(s) 109, 335, 392
AsiGI ACCGGT 1 cut(s) 303
Asp718I GGTACC 1 cut(s) 514
AspLEI GCGC 3 cut(s) 145, 653, 1128
AspS9I GGNCC 2 cut(s) 248, 306
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 170
AvaII GGWCC 2 cut(s) 248, 306
BamHI GGATCC 1 cut(s) 711
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 914
BarI GAAGNNNNNNTAC 4 cut(s) 554, 586, 1003, 1035
BbsI GAAGAC 3 cut(s) 312, 337, 959
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 6 cut(s) 20, 118, 431, 620, 644, 1076
BccI CCATC 3 cut(s) 16, 80, 847
BciT130I CCWGG 1 cut(s) 916
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 1 cut(s) 432
BfmI CTRYAG 2 cut(s) 1076, 1090
BglII AGATCT 1 cut(s) 229
BisI GCNGC 6 cut(s) 9, 132, 420, 609, 633, 1090
BlsI GCNGC 6 cut(s) 10, 133, 421, 610, 634, 1091
Bme1390I CCNGG 2 cut(s) 432, 916
Bme18I GGWCC 2 cut(s) 248, 306
BmgT120I GGNCC 2 cut(s) 248, 306
BmiI GGNNCC 3 cut(s) 27, 516, 713
BmrFI CCNGG 2 cut(s) 432, 916
BmrI ACTGGG 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 717
BpiI GAAGAC 3 cut(s) 312, 337, 959
BplI GAGNNNNNCTC 2 cut(s) 797, 829
Bpu10I CCTNAGC 1 cut(s) 804
BpuEI CTTGAG 2 cut(s) 639, 1106
BpuMI CCSGG 1 cut(s) 432
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 686
BsaJI CCNNGG 5 cut(s) 431, 442, 621, 846, 914
BsaWI WCCGGW 1 cut(s) 303
BsaXI ACNNNNNCTCC 4 cut(s) 826, 849, 856, 879
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 1078
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 3 cut(s) 506, 712, 827
Bse3DI GCAATG 1 cut(s) 373
Bse8I GATNNNNATC 1 cut(s) 686
BseBI CCWGG 1 cut(s) 916
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 5 cut(s) 431, 442, 621, 846, 914
BseGI GGATG 1 cut(s) 67
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 2 cut(s) 77, 1078
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 3 cut(s) 795, 1009, 1118
BseNI ACTGG 3 cut(s) 506, 712, 827
BseXI GCAGC 6 cut(s) 20, 118, 431, 620, 644, 1076
Bsh1236I CGCG 2 cut(s) 331, 651
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 771
BsiSI CCGG 3 cut(s) 304, 431, 545
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 2 cut(s) 77, 1078
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 4 cut(s) 317, 481, 635, 931
BsnI GGCC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 914
Bsp1407I TGTACA 1 cut(s) 831
Bsp143I GATC 3 cut(s) 229, 355, 711
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 1 cut(s) 1069
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 3 cut(s) 796, 1008, 1117
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 2 cut(s) 331, 651
BspLI GGNNCC 3 cut(s) 27, 516, 713
BspMAI CTGCAG 1 cut(s) 1094
BspPI GGATC 2 cut(s) 706, 719
BspT107I GGYRCC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 831
BsrI ACTGG 3 cut(s) 506, 712, 827
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 5 cut(s) 431, 442, 621, 846, 914
BssMI GATC 3 cut(s) 229, 355, 711
BssT1I CCWWGG 3 cut(s) 442, 621, 846
Bst2UI CCWGG 1 cut(s) 916
Bst4CI ACNGT 3 cut(s) 55, 80, 960
BstAPI GCANNNNNTGC 1 cut(s) 140
BstAUI TGTACA 1 cut(s) 831
BstC8I GCNNGC 5 cut(s) 364, 649, 696, 979, 1073
BstDEI CTNAG 4 cut(s) 804, 971, 995, 1104
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 2 cut(s) 331, 651
BstHHI GCGC 3 cut(s) 145, 653, 1128
BstKTI GATC 3 cut(s) 232, 358, 714
BstMBI GATC 3 cut(s) 229, 355, 711
BstMWI GCNNNNNNNGC 3 cut(s) 96, 140, 608
BstNI CCWGG 1 cut(s) 916
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 430, 914
BstSFI CTRYAG 2 cut(s) 1076, 1090
BstUI CGCG 2 cut(s) 331, 651
BstV1I GCAGC 6 cut(s) 20, 118, 431, 620, 644, 1076
BstV2I GAAGAC 3 cut(s) 312, 337, 959
BstX2I RGATCY 2 cut(s) 229, 711
BstXI CCANNNNNNTGG 3 cut(s) 58, 628, 853
BstYI RGATCY 2 cut(s) 229, 711
Bsu15I ATCGAT 1 cut(s) 771
BsuRI GGCC 1 cut(s) 447
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 1 cut(s) 67
BtsIMutI CAGTG 4 cut(s) 60, 499, 834, 956
Cac8I GCNNGC 5 cut(s) 364, 649, 696, 979, 1073
CfoI GCGC 3 cut(s) 145, 653, 1128
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 2 cut(s) 248, 306
ClaI ATCGAT 1 cut(s) 771
CpoI CGGWCCG 1 cut(s) 306
CseI GACGC 2 cut(s) 337, 888
Csp6I GTAC 4 cut(s) 301, 515, 832, 1101
CspAI ACCGGT 1 cut(s) 303
CspI CGGWCCG 1 cut(s) 306
CviAII CATG 7 cut(s) 172, 316, 443, 473, 549, 628, 982
CviQI GTAC 4 cut(s) 301, 515, 832, 1101
DdeI CTNAG 4 cut(s) 804, 971, 995, 1104
DpnI GATC 3 cut(s) 231, 357, 713
DpnII GATC 3 cut(s) 229, 355, 711
EaeI YGGCCR 1 cut(s) 445
Eco130I CCWWGG 3 cut(s) 442, 621, 846
Eco24I GRGCYC 1 cut(s) 914
Eco47I GGWCC 2 cut(s) 248, 306
Eco57I CTGAAG 2 cut(s) 606, 819
EcoRI GAATTC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 914
EcoT14I CCWWGG 3 cut(s) 442, 621, 846
EcoT22I ATGCAT 2 cut(s) 217, 317
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 3 cut(s) 442, 621, 846
FaeI CATG 7 cut(s) 175, 319, 446, 476, 552, 631, 985
FaqI GGGAC 1 cut(s) 686
FatI CATG 7 cut(s) 171, 315, 442, 472, 548, 627, 981
FauI CCCGC 1 cut(s) 1076
FauNDI CATATG 2 cut(s) 490, 1130
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 6 cut(s) 9, 132, 420, 609, 633, 1090
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 6 cut(s) 9, 132, 420, 609, 633, 1090
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 3 cut(s) 144, 652, 1127
GluI GCNGC 6 cut(s) 9, 132, 420, 609, 633, 1090
HaeIII GGCC 1 cut(s) 447
HapII CCGG 3 cut(s) 304, 431, 545
HgaI GACGC 2 cut(s) 337, 888
HhaI GCGC 3 cut(s) 145, 653, 1128
Hin1II CATG 7 cut(s) 175, 319, 446, 476, 552, 631, 985
Hin6I GCGC 3 cut(s) 143, 651, 1126
HinP1I GCGC 3 cut(s) 143, 651, 1126
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 4 cut(s) 565, 812, 902, 1082
HpaII CCGG 3 cut(s) 304, 431, 545
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 4 cut(s) 811, 832, 834, 1058
Hpy188I TCNGA 7 cut(s) 72, 310, 402, 799, 943, 998, 1010
Hpy188III TCNNGA 1 cut(s) 656
Hpy8I GTNNAC 4 cut(s) 811, 832, 834, 1058
HpyAV CCTTC 2 cut(s) 779, 1091
HpyCH4III ACNGT 3 cut(s) 55, 80, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 140, 608
HpyF3I CTNAG 4 cut(s) 804, 971, 995, 1104
Hsp92II CATG 7 cut(s) 175, 319, 446, 476, 552, 631, 985
HspAI GCGC 3 cut(s) 143, 651, 1126
KpnI GGTACC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 229, 355, 711
Lsp1109I GCAGC 6 cut(s) 20, 118, 431, 620, 644, 1076
MaeIII GTNAC 1 cut(s) 1046
MalI GATC 3 cut(s) 231, 357, 713
MboI GATC 3 cut(s) 229, 355, 711
MboII GAAGA 9 cut(s) 192, 195, 312, 337, 477, 533, 574, 599, 964
MflI RGATCY 2 cut(s) 229, 711
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 5 cut(s) 109, 288, 335, 392, 614
MluI ACGCGT 1 cut(s) 329
MlyI GAGTC 2 cut(s) 806, 1091
MmeI TCCRAC 3 cut(s) 226, 425, 960
MnlI CCTC 6 cut(s) 408, 475, 726, 799, 850, 979
Mph1103I ATGCAT 2 cut(s) 217, 317
MseI TTAA 4 cut(s) 291, 351, 597, 1035
MslI CAYNNNNRTG 2 cut(s) 723, 773
MspA1I CMGCKG 2 cut(s) 422, 698
MspI CCGG 3 cut(s) 304, 431, 545
MspR9I CCNGG 2 cut(s) 432, 916
Mva1269I GAATGC 4 cut(s) 317, 481, 635, 931
MvaI CCWGG 1 cut(s) 916
MvnI CGCG 2 cut(s) 331, 651
MwoI GCNNNNNNNGC 3 cut(s) 96, 140, 608
NciI CCSGG 1 cut(s) 432
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 2 cut(s) 490, 1130
NdeII GATC 3 cut(s) 229, 355, 711
NlaIII CATG 7 cut(s) 175, 319, 446, 476, 552, 631, 985
NlaIV GGNNCC 3 cut(s) 27, 516, 713
NmuCI GTSAC 1 cut(s) 1046
NsbI TGCGCA 1 cut(s) 144
NsiI ATGCAT 2 cut(s) 217, 317
NspI RCATGY 1 cut(s) 319
PctI GAATGC 4 cut(s) 317, 481, 635, 931
PfeI GAWTC 2 cut(s) 565, 902
PflFI GACNNNGTC 1 cut(s) 958
PinAI ACCGGT 1 cut(s) 303
PkrI GCNGC 6 cut(s) 10, 133, 421, 610, 634, 1091
PleI GAGTC 2 cut(s) 806, 1090
PpsI GAGTC 2 cut(s) 806, 1090
Psp6I CCWGG 1 cut(s) 914
PspGI CCWGG 1 cut(s) 914
PspN4I GGNNCC 3 cut(s) 27, 516, 713
PspPI GGNCC 2 cut(s) 248, 306
PstI CTGCAG 1 cut(s) 1094
PsuI RGATCY 2 cut(s) 229, 711
PsyI GACNNNGTC 1 cut(s) 958
PvuII CAGCTG 2 cut(s) 422, 698
RsaI GTAC 4 cut(s) 302, 516, 833, 1102
RsaNI GTAC 4 cut(s) 301, 515, 832, 1101
RseI CAYNNNNRTG 2 cut(s) 723, 773
Rsr2I CGGWCCG 1 cut(s) 306
RsrII CGGWCCG 1 cut(s) 306
SaqAI TTAA 4 cut(s) 291, 351, 597, 1035
SatI GCNGC 6 cut(s) 9, 132, 420, 609, 633, 1090
Sau3AI GATC 3 cut(s) 229, 355, 711
Sau96I GGNCC 2 cut(s) 248, 306
SchI GAGTC 2 cut(s) 806, 1091
ScrFI CCNGG 2 cut(s) 432, 916
SduI GDGCHC 1 cut(s) 914
SfcI CTRYAG 2 cut(s) 1076, 1090
SinI GGWCC 2 cut(s) 248, 306
SmiMI CAYNNNNRTG 2 cut(s) 723, 773
SmlI CTYRAG 2 cut(s) 654, 1121
SmoI CTYRAG 2 cut(s) 654, 1121
Sse9I AATT 5 cut(s) 109, 288, 335, 392, 614
SsiI CCGC 1 cut(s) 1069
StyD4I CCNGG 2 cut(s) 430, 914
StyI CCWWGG 3 cut(s) 442, 621, 846
TaaI ACNGT 3 cut(s) 55, 80, 960
TaqI TCGA 4 cut(s) 747, 771, 891, 962
TasI AATT 5 cut(s) 109, 288, 335, 392, 614
TatI WGTACW 1 cut(s) 831
TfiI GAWTC 2 cut(s) 565, 902
Tru1I TTAA 4 cut(s) 291, 351, 597, 1035
Tru9I TTAA 4 cut(s) 291, 351, 597, 1035
TscAI CASTG 4 cut(s) 60, 506, 834, 963
TseFI GTSAC 1 cut(s) 1046
TseI GCWGC 6 cut(s) 8, 131, 419, 608, 632, 1089
Tsp45I GTSAC 1 cut(s) 1046
TspDTI ATGAA 3 cut(s) 89, 188, 477
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 4 cut(s) 60, 506, 834, 963
Tth111I GACNNNGTC 1 cut(s) 958
VpaK11BI GGWCC 2 cut(s) 248, 306
XapI RAATTY 3 cut(s) 109, 335, 392
XceI RCATGY 1 cut(s) 319
Zsp2I ATGCAT 2 cut(s) 217, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.