Rh4CG459900

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018707
Physical Location & Seq
Reverse (-)
69088659 .. 69090048
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG459900.1

Sequence Viewer

Length: 1101 bp
ATGAGCAACAAGGCAACCCCAGTGCTGGAAGCACATCCAATGAACGGTGGAGATGGCACATACAGCTACACCAAAAACTCCTATTACCAAAGAGAAGCTGCAAATGTTGCGCAAACTCTAATCGGTGATGTGATAGGCATGAAGCTTGACATAAACGAGATTACTTCTTCTTCGACCAATGCATTTTGTATAGCAGATTTGGGGTGTTCAGTTGGACCAAACAGTTTTGTTTCTGTGGAGAACATACTGGAAGCTATTAAACATAAGTACCAGTCCGGATGCATGTCTTCCCAAATGCCTGAATTCCAAGTCTTCTTTAATGATCATGCAGGCAATGATTTCAATACCCTATTTGTAAATTTCCCTCCGACAAGGCCCTACTTTGCAGCTGGTGTGCCGGGTTCTTTCCATGGCCGATTATTCCCCAAGTCCGCTCTCCATTTTGTGCATTCTTCATATGCAACCCACTGGCTCTCAAAGGTACCAGAAGAACTGACAGACAAGAACTCTCCGGCATGGAACAAAGGGAAGATTCACTACACAACTGCCCCTGAAGAAGTAGTTAATGCTTATGCAGCTCAATTTGCCAAGGACATGGCAACATTCTTGGAAGCTCGCGCTCAAGAGCTTGTGGTTGGTGGAATGATGATGCTAATCATGCAAGCTGTCCCAACTGGGATCCCTCATTCCCGTGTTCCAAATGGAGTGATGTTTGATTTTCTGGGGTCTATTCTCATGGATATGGCAAAGGAAGGAGTTATATCTGAAGCTGAGGTGGACTCATTCAACTTGCCAGTGTACACTACCTCTCCCAAGGAGATGGGAGAGCTGGTAGAGAGAAATGGATGCTTTAGCATAGAGCGAATGGAGTCAACGAGCCCCTGGCTTGATTCCAAGCATTCGAACTCCAACTACGGGAAGACACTTTCGATGACGCTGAGAGCTGGCATGGAGGGAGTTCTCAAAAGCCATTTTGGAAGTGAGATTACCAATCAACTGTTTAATAGGCTCTGTGACCAAAGTGGACAACTTACCCACCAACTAGAGGAGTCCTGCTGCAGAGAAGGAACTCAGTTGTTTGTTGCTCTCAAGCGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

366

Amino Acids

40.4

Weight (kDa)

5.74

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 365 4.2e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 111
Acc65I GGTACC 1 cut(s) 481
AccB1I GGYRCC 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 434
AccII CGCG 1 cut(s) 618
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 1 cut(s) 432
AclWI GGATC 2 cut(s) 673, 686
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 302, 358
AcuI CTGAAG 2 cut(s) 573, 786
AfaI GTAC 3 cut(s) 269, 483, 800
AfiI CCNNNNNNNGG 4 cut(s) 25, 44, 915, 1045
AgsI TTSAA 2 cut(s) 343, 787
AjnI CCWGG 1 cut(s) 881
AlwI GGATC 2 cut(s) 673, 686
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 2 cut(s) 374, 412
ApeKI GCWGC 4 cut(s) 98, 386, 575, 1056
ApoI RAATTY 2 cut(s) 302, 358
Asp718I GGTACC 1 cut(s) 481
AspLEI GCGC 3 cut(s) 112, 620, 1095
AspS9I GGNCC 2 cut(s) 215, 375
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 902
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 251, 284
BamHI GGATCC 1 cut(s) 678
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 881
BarI GAAGNNNNNNTAC 4 cut(s) 521, 553, 970, 1002
BbsI GAAGAC 3 cut(s) 279, 304, 926
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 4 cut(s) 85, 398, 587, 1043
BccI CCATC 2 cut(s) 47, 814
BciT130I CCWGG 1 cut(s) 883
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 399
BfaI CTAG 1 cut(s) 1043
BfmI CTRYAG 1 cut(s) 1057
BisI GCNGC 4 cut(s) 99, 387, 576, 1057
BlsI GCNGC 4 cut(s) 100, 388, 577, 1058
Bme1390I CCNGG 2 cut(s) 399, 883
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 215, 375
BmiI GGNNCC 2 cut(s) 483, 680
BmrFI CCNGG 2 cut(s) 399, 883
BmrI ACTGGG 2 cut(s) 14, 684
BmsI GCATC 3 cut(s) 269, 639, 836
BmuI ACTGGG 2 cut(s) 14, 684
BpiI GAAGAC 3 cut(s) 279, 304, 926
BplI GAGNNNNNCTC 2 cut(s) 764, 796
Bpu10I CCTNAGC 1 cut(s) 771
Bpu14I TTCGAA 1 cut(s) 902
BpuEI CTTGAG 2 cut(s) 606, 1073
BpuMI CCSGG 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 653
BsaJI CCNNGG 4 cut(s) 409, 588, 813, 881
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 4 cut(s) 793, 816, 823, 846
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 44, 915, 1045
Bse1I ACTGG 6 cut(s) 20, 252, 271, 473, 679, 794
Bse3DI GCAATG 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 4 cut(s) 409, 588, 813, 881
BseGI GGATG 3 cut(s) 34, 284, 851
BseJI GATNNNNATC 1 cut(s) 653
BseLI CCNNNNNNNGG 4 cut(s) 25, 44, 915, 1045
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 3 cut(s) 762, 929, 1085
BseNI ACTGG 6 cut(s) 20, 252, 271, 473, 679, 794
BseRI GAGGAG 1 cut(s) 1061
BseXI GCAGC 4 cut(s) 85, 398, 587, 1043
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 2 cut(s) 376, 414
BshNI GGYRCC 1 cut(s) 481
BsiSI CCGG 3 cut(s) 276, 398, 512
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 4 cut(s) 25, 44, 915, 1045
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 2 cut(s) 448, 898
BsnI GGCC 2 cut(s) 376, 414
Bsp119I TTCGAA 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 881
Bsp13I TCCGGA 1 cut(s) 275
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 2 cut(s) 322, 678
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 1 cut(s) 432
BspANI GGCC 2 cut(s) 376, 414
BspCNI CTCAG 3 cut(s) 763, 930, 1084
BspEI TCCGGA 1 cut(s) 275
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 483, 680
BspMAI CTGCAG 1 cut(s) 1061
BspPI GGATC 2 cut(s) 673, 686
BspT104I TTCGAA 1 cut(s) 902
BspT107I GGYRCC 1 cut(s) 481
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 340
BsrGI TGTACA 1 cut(s) 798
BsrI ACTGG 6 cut(s) 20, 252, 271, 473, 679, 794
BssECI CCNNGG 4 cut(s) 409, 588, 813, 881
BssMI GATC 2 cut(s) 322, 678
BssT1I CCWWGG 3 cut(s) 409, 588, 813
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 3 cut(s) 47, 224, 999
BstAPI GCANNNNNTGC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 798
BstBI TTCGAA 1 cut(s) 902
BstC8I GCNNGC 4 cut(s) 331, 616, 663, 946
BstDEI CTNAG 3 cut(s) 771, 938, 1071
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 34, 284, 851
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 3 cut(s) 112, 620, 1095
BstKTI GATC 2 cut(s) 325, 681
BstMBI GATC 2 cut(s) 322, 678
BstMWI GCNNNNNNNGC 5 cut(s) 63, 107, 575, 584, 658
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 2 cut(s) 397, 881
BstSFI CTRYAG 1 cut(s) 1057
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 4 cut(s) 85, 398, 587, 1043
BstV2I GAAGAC 3 cut(s) 279, 304, 926
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 2 cut(s) 595, 820
BstYI RGATCY 1 cut(s) 678
BsuRI GGCC 2 cut(s) 376, 414
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 3 cut(s) 34, 284, 851
BtsIMutI CAGTG 3 cut(s) 27, 466, 801
Cac8I GCNNGC 4 cut(s) 331, 616, 663, 946
CfoI GCGC 3 cut(s) 112, 620, 1095
Cfr13I GGNCC 2 cut(s) 215, 375
CseI GACGC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 268, 482, 799
CviAII CATG 9 cut(s) 139, 283, 326, 410, 516, 595, 658, 736, 949
CviQI GTAC 3 cut(s) 268, 482, 799
DdeI CTNAG 3 cut(s) 771, 938, 1071
DpnI GATC 2 cut(s) 324, 680
DpnII GATC 2 cut(s) 322, 678
EaeI YGGCCR 1 cut(s) 412
Eco130I CCWWGG 3 cut(s) 409, 588, 813
Eco24I GRGCYC 1 cut(s) 881
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 573, 786
EcoO109I RGGNCCY 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 881
EcoT14I CCWWGG 3 cut(s) 409, 588, 813
EcoT22I ATGCAT 2 cut(s) 184, 284
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 3 cut(s) 409, 588, 813
FaeI CATG 9 cut(s) 142, 286, 329, 413, 519, 598, 661, 739, 952
FaqI GGGAC 1 cut(s) 653
FatI CATG 9 cut(s) 138, 282, 325, 409, 515, 594, 657, 735, 948
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 99, 387, 576, 1057
FokI GGATG 3 cut(s) 21, 291, 858
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 4 cut(s) 99, 387, 576, 1057
FspBI CTAG 1 cut(s) 1043
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 3 cut(s) 111, 619, 1094
GluI GCNGC 4 cut(s) 99, 387, 576, 1057
HaeIII GGCC 2 cut(s) 376, 414
HapII CCGG 3 cut(s) 276, 398, 512
HgaI GACGC 1 cut(s) 943
HhaI GCGC 3 cut(s) 112, 620, 1095
Hin1II CATG 9 cut(s) 142, 286, 329, 413, 519, 598, 661, 739, 952
Hin6I GCGC 3 cut(s) 110, 618, 1093
HinP1I GCGC 3 cut(s) 110, 618, 1093
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 5 cut(s) 532, 779, 869, 890, 1049
HpaII CCGG 3 cut(s) 276, 398, 512
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 5 cut(s) 778, 799, 801, 873, 1025
Hpy188I TCNGA 2 cut(s) 369, 766
Hpy188III TCNNGA 2 cut(s) 276, 623
Hpy8I GTNNAC 5 cut(s) 778, 799, 801, 873, 1025
HpyAV CCTTC 2 cut(s) 746, 1058
HpyCH4III ACNGT 3 cut(s) 47, 224, 999
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 107, 575, 584, 658
HpyF3I CTNAG 3 cut(s) 771, 938, 1071
Hsp92II CATG 9 cut(s) 142, 286, 329, 413, 519, 598, 661, 739, 952
HspAI GCGC 3 cut(s) 110, 618, 1093
Kpn2I TCCGGA 1 cut(s) 275
KpnI GGTACC 1 cut(s) 485
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 322, 678
Lsp1109I GCAGC 4 cut(s) 85, 398, 587, 1043
LweI GCATC 3 cut(s) 269, 639, 836
MaeI CTAG 1 cut(s) 1043
MaeIII GTNAC 1 cut(s) 1013
MalI GATC 2 cut(s) 324, 680
MbiI CCGCTC 1 cut(s) 434
MboI GATC 2 cut(s) 322, 678
MboII GAAGA 9 cut(s) 159, 162, 279, 304, 444, 500, 541, 566, 931
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 881
MluCI AATT 3 cut(s) 302, 358, 581
MlyI GAGTC 3 cut(s) 773, 878, 1058
MmeI TCCRAC 3 cut(s) 193, 392, 933
MnlI CCTC 6 cut(s) 375, 693, 766, 817, 946, 1039
Mph1103I ATGCAT 2 cut(s) 184, 284
MroI TCCGGA 1 cut(s) 275
MseI TTAA 4 cut(s) 258, 318, 564, 1002
MslI CAYNNNNRTG 2 cut(s) 690, 740
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 276, 398, 512
MspR9I CCNGG 2 cut(s) 399, 883
Mva1269I GAATGC 2 cut(s) 448, 898
MvaI CCWGG 1 cut(s) 883
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 5 cut(s) 63, 107, 575, 584, 658
NciI CCSGG 1 cut(s) 399
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 457
NdeII GATC 2 cut(s) 322, 678
NlaIII CATG 9 cut(s) 142, 286, 329, 413, 519, 598, 661, 739, 952
NlaIV GGNNCC 2 cut(s) 483, 680
NmuCI GTSAC 1 cut(s) 1013
NsbI TGCGCA 1 cut(s) 111
NsiI ATGCAT 2 cut(s) 184, 284
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 902
PctI GAATGC 2 cut(s) 448, 898
PfeI GAWTC 2 cut(s) 532, 890
PkrI GCNGC 4 cut(s) 100, 388, 577, 1058
PleI GAGTC 3 cut(s) 773, 877, 1057
PpsI GAGTC 3 cut(s) 773, 877, 1057
Psp6I CCWGG 1 cut(s) 881
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 2 cut(s) 483, 680
PspPI GGNCC 2 cut(s) 215, 375
PsrI GAACNNNNNNTAC 2 cut(s) 896, 928
PstI CTGCAG 1 cut(s) 1061
PsuI RGATCY 1 cut(s) 678
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 269, 483, 800
RsaNI GTAC 3 cut(s) 268, 482, 799
RseI CAYNNNNRTG 2 cut(s) 690, 740
SaqAI TTAA 4 cut(s) 258, 318, 564, 1002
SatI GCNGC 4 cut(s) 99, 387, 576, 1057
Sau3AI GATC 2 cut(s) 322, 678
Sau96I GGNCC 2 cut(s) 215, 375
SchI GAGTC 3 cut(s) 773, 878, 1058
ScrFI CCNGG 2 cut(s) 399, 883
SduI GDGCHC 1 cut(s) 881
SfaNI GCATC 3 cut(s) 269, 639, 836
SfcI CTRYAG 1 cut(s) 1057
SfuI TTCGAA 1 cut(s) 902
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 690, 740
SmlI CTYRAG 2 cut(s) 621, 1088
SmoI CTYRAG 2 cut(s) 621, 1088
Sse9I AATT 3 cut(s) 302, 358, 581
SsiI CCGC 1 cut(s) 432
SspMI CTAG 1 cut(s) 1043
StyD4I CCNGG 2 cut(s) 397, 881
StyI CCWWGG 3 cut(s) 409, 588, 813
TaaI ACNGT 3 cut(s) 47, 224, 999
TaqI TCGA 3 cut(s) 173, 902, 929
TasI AATT 3 cut(s) 302, 358, 581
TatI WGTACW 1 cut(s) 798
TfiI GAWTC 2 cut(s) 532, 890
Tru1I TTAA 4 cut(s) 258, 318, 564, 1002
Tru9I TTAA 4 cut(s) 258, 318, 564, 1002
TscAI CASTG 3 cut(s) 27, 473, 801
TseFI GTSAC 1 cut(s) 1013
TseI GCWGC 4 cut(s) 98, 386, 575, 1056
Tsp45I GTSAC 1 cut(s) 1013
TspDTI ATGAA 3 cut(s) 56, 155, 444
TspRI CASTG 3 cut(s) 27, 473, 801
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 302, 358
XceI RCATGY 1 cut(s) 286
XspI CTAG 1 cut(s) 1043
Zsp2I ATGCAT 2 cut(s) 184, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.