RchiOBHm_Chr4g0446481

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018707
Physical Location & Seq
Reverse (-)
66618313 .. 66619705
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41400

Sequence Viewer

Length: 1104 bp
ATGAGCAACAAGGCAAACCCAGTGCTGGAAGCACATCCAATGAACGGTGGAGATGGCACATACAGCTACACCAAAAACTCCTATTACCAAAGAGAAGCTGCAAATGTTGCGCAAACTCTAATCGGTGATGTGATAGGCATGAAGCTTGACATAAACAAGATTACTTCTTCGACCAATGCATTTTGTATAGCAGATTTGGGGTGTTCAGTTGGACCAAACAGTTTTGTTTCTGTGGAGAACATACTGGAAGCTATTAAACATAAGTACCAGTCCGGATGCATGTCTTCCCAAATGCCTGAATTCCAAGTTTTCTTTAATGATCATGCAGGCAATGATTTCAATACCCTATTTGCAAATTTCCCTCCGACAAGGCCCTACTTTGCAGCTGGTGTGCCGGGTTCTTTCCATGGCCGATTATTCCCCAAGTCCTCTCTCCATTTCGTGCATTCTTCATATGCAACCCACTGGCTCTCAAAGGTACCAGAAGAACTGACAGACAAGAACTCTCCGGCATGGAACAAAGGGAAGATTCACTACACAACTGCCCCCGAAGAAGTAGTTAATGCTTATGCAGCTCAATTTGCCAAGGACATGGCAACATTCTTGGAAGCTCGCGCTCAAGAGCTTGTGGTTGGTGGAATGATGATGCTAATCATGCAAGCTGTCCCAACTGGGATCCCTCATTCCCGTGTTCCAAATGGAGTGATGTTTGATTTTCTGGGGTCTATTCTCATGGATATGGCAAAGGAAGGAGTTATATCTGAAGCTGAGGTGGACTCATTCAACTTGCCAGTGTACACTACCTCTCCCAAGGAGATAGGAGAGCTGGTAGAGAGAAATGGATGCTTTAGCATAGAGCGAATGGAGTCAACGAGCCCCTGGCTTGCTTCCAAGCATTCGAACTCCAACTACGGGAAGACACTGTCGATGTCGCTAAGAGCTGGCATGGAGGGAGTTTTCAAAAGCCATTTTGGAAGTGAGATTACCAATCAACTGTTTAATAGGCTCTGTGACCAAAGTGGACAACTTACCCACCAACTGGAGGAGTCCTACTCCTGCTGCAGAGAAGGAACTCAGTTGTTTGTTGCTCTCAAGCGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

367

Amino Acids

40.52

Weight (kDa)

6.06

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 366 6e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 111
Acc65I GGTACC 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 478
AccB7I CCANNNNNTGG 1 cut(s) 1039
AccII CGCG 1 cut(s) 615
AccIII TCCGGA 1 cut(s) 272
AclWI GGATC 2 cut(s) 670, 683
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 299, 355
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 3 cut(s) 266, 480, 797
AfiI CCNNNNNNNGG 5 cut(s) 25, 44, 912, 1039, 1042
AgsI TTSAA 3 cut(s) 340, 784, 961
AjnI CCWGG 1 cut(s) 878
AlwI GGATC 2 cut(s) 670, 683
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 2 cut(s) 371, 409
ApeKI GCWGC 4 cut(s) 98, 383, 572, 1059
ApoI RAATTY 2 cut(s) 299, 355
Asp718I GGTACC 1 cut(s) 478
AspLEI GCGC 3 cut(s) 112, 617, 1098
AspS9I GGNCC 2 cut(s) 212, 372
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 899
AvaII GGWCC 1 cut(s) 212
BaeI ACNNNNGTAYC 2 cut(s) 248, 281
BamHI GGATCC 1 cut(s) 675
BanI GGYRCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 878
BarI GAAGNNNNNNTAC 4 cut(s) 518, 550, 967, 999
BbsI GAAGAC 2 cut(s) 276, 923
BbvCI CCTCAGC 1 cut(s) 768
BbvI GCAGC 4 cut(s) 85, 395, 584, 1046
BccI CCATC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 880
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 396
BfmI CTRYAG 1 cut(s) 1060
BisI GCNGC 4 cut(s) 99, 384, 573, 1060
BlsI GCNGC 4 cut(s) 100, 385, 574, 1061
Bme1390I CCNGG 2 cut(s) 396, 880
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 212, 372
BmiI GGNNCC 2 cut(s) 480, 677
BmrFI CCNGG 2 cut(s) 396, 880
BmrI ACTGGG 2 cut(s) 14, 681
BmsI GCATC 3 cut(s) 266, 636, 833
BmuI ACTGGG 2 cut(s) 14, 681
BpiI GAAGAC 2 cut(s) 276, 923
BplI GAGNNNNNCTC 4 cut(s) 761, 793, 1037, 1069
BpmI CTGGAG 1 cut(s) 1061
Bpu10I CCTNAGC 1 cut(s) 768
Bpu14I TTCGAA 1 cut(s) 899
BpuEI CTTGAG 2 cut(s) 603, 1076
BpuMI CCSGG 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 650
BsaJI CCNNGG 4 cut(s) 406, 585, 810, 878
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 4 cut(s) 790, 813, 820, 843
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 44, 912, 1039, 1042
Bse1I ACTGG 7 cut(s) 20, 249, 268, 470, 676, 791, 1044
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 4 cut(s) 406, 585, 810, 878
BseGI GGATG 3 cut(s) 34, 281, 848
BseJI GATNNNNATC 1 cut(s) 650
BseLI CCNNNNNNNGG 5 cut(s) 25, 44, 912, 1039, 1042
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 759, 1088
BseNI ACTGG 7 cut(s) 20, 249, 268, 470, 676, 791, 1044
BseRI GAGGAG 1 cut(s) 1058
BseXI GCAGC 4 cut(s) 85, 395, 584, 1046
Bsh1236I CGCG 1 cut(s) 615
BshFI GGCC 2 cut(s) 373, 411
BshNI GGYRCC 1 cut(s) 478
BsiSI CCGG 3 cut(s) 273, 395, 509
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 5 cut(s) 25, 44, 912, 1039, 1042
BsmFI GGGAC 1 cut(s) 650
BsmI GAATGC 2 cut(s) 445, 895
BsnI GGCC 2 cut(s) 373, 411
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 1 cut(s) 878
Bsp13I TCCGGA 1 cut(s) 272
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 2 cut(s) 319, 675
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 2 cut(s) 373, 411
BspCNI CTCAG 2 cut(s) 760, 1087
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 480, 677
BspMAI CTGCAG 1 cut(s) 1064
BspPI GGATC 2 cut(s) 670, 683
BspT104I TTCGAA 1 cut(s) 899
BspT107I GGYRCC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 7 cut(s) 20, 249, 268, 470, 676, 791, 1044
BssECI CCNNGG 4 cut(s) 406, 585, 810, 878
BssMI GATC 2 cut(s) 319, 675
BssT1I CCWWGG 3 cut(s) 406, 585, 810
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 4 cut(s) 47, 221, 924, 996
BstAPI GCANNNNNTGC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 795
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 5 cut(s) 328, 613, 660, 885, 943
BstDEI CTNAG 3 cut(s) 768, 935, 1074
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 3 cut(s) 34, 281, 848
BstFNI CGCG 1 cut(s) 615
BstHHI GCGC 3 cut(s) 112, 617, 1098
BstKTI GATC 2 cut(s) 322, 678
BstMBI GATC 2 cut(s) 319, 675
BstMWI GCNNNNNNNGC 5 cut(s) 63, 107, 572, 581, 655
BstNI CCWGG 1 cut(s) 880
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 394, 878
BstSFI CTRYAG 1 cut(s) 1060
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 4 cut(s) 85, 395, 584, 1046
BstV2I GAAGAC 2 cut(s) 276, 923
BstX2I RGATCY 1 cut(s) 675
BstXI CCANNNNNNTGG 1 cut(s) 592
BstYI RGATCY 1 cut(s) 675
BsuRI GGCC 2 cut(s) 373, 411
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 3 cut(s) 34, 281, 848
BtsIMutI CAGTG 4 cut(s) 27, 463, 798, 920
Cac8I GCNNGC 5 cut(s) 328, 613, 660, 885, 943
CfoI GCGC 3 cut(s) 112, 617, 1098
Cfr13I GGNCC 2 cut(s) 212, 372
Csp6I GTAC 3 cut(s) 265, 479, 796
CviAII CATG 9 cut(s) 139, 280, 323, 407, 513, 592, 655, 733, 946
CviQI GTAC 3 cut(s) 265, 479, 796
DdeI CTNAG 3 cut(s) 768, 935, 1074
DpnI GATC 2 cut(s) 321, 677
DpnII GATC 2 cut(s) 319, 675
EaeI YGGCCR 1 cut(s) 409
Eco130I CCWWGG 3 cut(s) 406, 585, 810
Eco24I GRGCYC 1 cut(s) 878
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 783
EcoO109I RGGNCCY 1 cut(s) 372
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 878
EcoT14I CCWWGG 3 cut(s) 406, 585, 810
EcoT22I ATGCAT 2 cut(s) 181, 281
EcoT38I GRGCYC 1 cut(s) 878
ErhI CCWWGG 3 cut(s) 406, 585, 810
FaeI CATG 9 cut(s) 142, 283, 326, 410, 516, 595, 658, 736, 949
FaqI GGGAC 1 cut(s) 650
FatI CATG 9 cut(s) 138, 279, 322, 406, 512, 591, 654, 732, 945
FauNDI CATATG 1 cut(s) 454
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 4 cut(s) 99, 384, 573, 1060
FokI GGATG 3 cut(s) 21, 288, 855
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 4 cut(s) 99, 384, 573, 1060
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 3 cut(s) 111, 616, 1097
GluI GCNGC 4 cut(s) 99, 384, 573, 1060
GsuI CTGGAG 1 cut(s) 1061
HaeIII GGCC 2 cut(s) 373, 411
HapII CCGG 3 cut(s) 273, 395, 509
HhaI GCGC 3 cut(s) 112, 617, 1098
Hin1II CATG 9 cut(s) 142, 283, 326, 410, 516, 595, 658, 736, 949
Hin6I GCGC 3 cut(s) 110, 615, 1096
HinP1I GCGC 3 cut(s) 110, 615, 1096
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 529, 776, 866, 1046
HpaII CCGG 3 cut(s) 273, 395, 509
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 5 cut(s) 775, 796, 798, 870, 1022
Hpy188I TCNGA 2 cut(s) 366, 763
Hpy188III TCNNGA 2 cut(s) 273, 620
Hpy8I GTNNAC 5 cut(s) 775, 796, 798, 870, 1022
HpyAV CCTTC 2 cut(s) 743, 1061
HpyCH4III ACNGT 4 cut(s) 47, 221, 924, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 107, 572, 581, 655
HpyF3I CTNAG 3 cut(s) 768, 935, 1074
Hsp92II CATG 9 cut(s) 142, 283, 326, 410, 516, 595, 658, 736, 949
HspAI GCGC 3 cut(s) 110, 615, 1096
Kpn2I TCCGGA 1 cut(s) 272
KpnI GGTACC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 2 cut(s) 319, 675
Lsp1109I GCAGC 4 cut(s) 85, 395, 584, 1046
LweI GCATC 3 cut(s) 266, 636, 833
MaeIII GTNAC 1 cut(s) 1010
MalI GATC 2 cut(s) 321, 677
MboI GATC 2 cut(s) 319, 675
MboII GAAGA 7 cut(s) 159, 276, 441, 497, 538, 563, 928
MflI RGATCY 1 cut(s) 675
MhlI GDGCHC 1 cut(s) 878
MluCI AATT 3 cut(s) 299, 355, 578
MlyI GAGTC 3 cut(s) 770, 875, 1055
MmeI TCCRAC 3 cut(s) 190, 389, 930
MnlI CCTC 7 cut(s) 372, 439, 690, 763, 814, 943, 1036
Mph1103I ATGCAT 2 cut(s) 181, 281
MroI TCCGGA 1 cut(s) 272
MseI TTAA 4 cut(s) 255, 315, 561, 999
MslI CAYNNNNRTG 2 cut(s) 687, 737
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 3 cut(s) 273, 395, 509
MspR9I CCNGG 2 cut(s) 396, 880
Mva1269I GAATGC 2 cut(s) 445, 895
MvaI CCWGG 1 cut(s) 880
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 5 cut(s) 63, 107, 572, 581, 655
NciI CCSGG 1 cut(s) 396
NcoI CCATGG 1 cut(s) 406
NdeI CATATG 1 cut(s) 454
NdeII GATC 2 cut(s) 319, 675
NlaIII CATG 9 cut(s) 142, 283, 326, 410, 516, 595, 658, 736, 949
NlaIV GGNNCC 2 cut(s) 480, 677
NmuCI GTSAC 1 cut(s) 1010
NsbI TGCGCA 1 cut(s) 111
NsiI ATGCAT 2 cut(s) 181, 281
NspI RCATGY 1 cut(s) 283
NspV TTCGAA 1 cut(s) 899
PctI GAATGC 2 cut(s) 445, 895
PfeI GAWTC 1 cut(s) 529
PflFI GACNNNGTC 1 cut(s) 922
PflMI CCANNNNNTGG 1 cut(s) 1039
PkrI GCNGC 4 cut(s) 100, 385, 574, 1061
PleI GAGTC 3 cut(s) 770, 874, 1054
PpsI GAGTC 3 cut(s) 770, 874, 1054
Psp6I CCWGG 1 cut(s) 878
PspGI CCWGG 1 cut(s) 878
PspN4I GGNNCC 2 cut(s) 480, 677
PspPI GGNCC 2 cut(s) 212, 372
PsrI GAACNNNNNNTAC 2 cut(s) 893, 925
PstI CTGCAG 1 cut(s) 1064
PsuI RGATCY 1 cut(s) 675
PsyI GACNNNGTC 1 cut(s) 922
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 3 cut(s) 266, 480, 797
RsaNI GTAC 3 cut(s) 265, 479, 796
RseI CAYNNNNRTG 2 cut(s) 687, 737
SaqAI TTAA 4 cut(s) 255, 315, 561, 999
SatI GCNGC 4 cut(s) 99, 384, 573, 1060
Sau3AI GATC 2 cut(s) 319, 675
Sau96I GGNCC 2 cut(s) 212, 372
SchI GAGTC 3 cut(s) 770, 875, 1055
ScrFI CCNGG 2 cut(s) 396, 880
SduI GDGCHC 1 cut(s) 878
SfaNI GCATC 3 cut(s) 266, 636, 833
SfcI CTRYAG 1 cut(s) 1060
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 687, 737
SmlI CTYRAG 2 cut(s) 618, 1091
SmoI CTYRAG 2 cut(s) 618, 1091
Sse9I AATT 3 cut(s) 299, 355, 578
StyD4I CCNGG 2 cut(s) 394, 878
StyI CCWWGG 3 cut(s) 406, 585, 810
TaaI ACNGT 4 cut(s) 47, 221, 924, 996
TaqI TCGA 3 cut(s) 170, 899, 926
TasI AATT 3 cut(s) 299, 355, 578
TatI WGTACW 1 cut(s) 795
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 4 cut(s) 255, 315, 561, 999
Tru9I TTAA 4 cut(s) 255, 315, 561, 999
TscAI CASTG 4 cut(s) 27, 470, 798, 927
TseFI GTSAC 1 cut(s) 1010
TseI GCWGC 4 cut(s) 98, 383, 572, 1059
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 3 cut(s) 56, 155, 441
TspRI CASTG 4 cut(s) 27, 470, 798, 927
Tth111I GACNNNGTC 1 cut(s) 922
Van91I CCANNNNNTGG 1 cut(s) 1039
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 2 cut(s) 299, 355
XceI RCATGY 1 cut(s) 283
Zsp2I ATGCAT 2 cut(s) 181, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.