pycom13g00610

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0018707
Physical Location & Seq
Forward (+)
386521 .. 388686
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00610.4

Sequence Viewer

Length: 1314 bp
ATGCTCTTTCTTTCTGTGTGGGACGATGCCGGGGAGAGAGGGGAATGTGTCGAGGCGGCGCCGCTAAAGCTTCATGCCATTGAATACTGGTCCCGAAAACGATCAATACAGGGACATTTTGTGTGCGTGTGCGCTATAAATTGCAGATATCTATCACTGACAACCTGCACAATTCGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGTTGAAGATGAGCATCAACGGAACCACGGTGCCGGAAGCATATCCGATGAACGGTGGAGATGGCGCATACAGCTACACCAAAAACTCCTATTTCCAGAGAGCTGCTGCAAATGTTTCAAGGAGTCTACTTGATGATGCAATAACAGAGAAGCTTGACATAGAGGACTTTTCTTGCAATCCAACCAACGCATTTCGGATAGCAGATTTGGGATGTTCAGTTGGACCAAACACTTTCTTTAGCGTCCAAAACATTCTGGAAGCAGTGAAACACAAGTACCAATCCCAATGCATCTCCTCCCAAATGCCTGAATTCCAAGTATTCTTCAGCGATCATGTGGCCAACGATTTTAACACCCTTTTTGCGTCTTTGCCTCCGGAAAGGCACTACTTTGCTGCTGGCGTGCCGGGGTCTTTCCACGGCCAGTTATTCCCCAAGTCCTCGCTCCATTTCGTGCATTCTTCGTATGCAGCTCACTGGCTCTCTAAGGTACCGGAGCAAGTGGTAGACAAGAACTCTCCGGCGTGGAACAGAGGGAAGATTTACTACACGACTTCCCCTGATGAAGTAGTTGATGCTTATGCAGCCCAATTTGGTAAAGACATGGCAGCATTCTTGGAAGCTCGGGCTAAAGAGCTTGTGGTGGGTGGAATGATGGTGATAATCATGCAAGCTATCCCAAATGGGACCCCTCCATCTCAGATTCCAAATGGCATCATGTTTGATTTTCTGGGGTCTACTCTCATGGAGATTGCCAAGGAAGGATTGATTAGCGAAGGAGAGGTGGACTCGTTCAACATTCCAACATACAACGCAACTCCGAAGGAAATGATGGAGGTGATCGAAAGAAATGGATGCTTCAACATAGCGAGAATTGAGTCGACAAGCCCATGGTTAAAAGCCGGTCATATGATCAATGGGCCAGGACTCACAATGCAGCTCAGAGCTGGCATGGAGGGAGTTTTCAAAACGCATTTTGGAACTAAGATAACGGACCAAGTGTTTGATAGGGTTTATGAAAAAAATGAAGAACTTATTCACAAAATAAAATCCAGCTGCAAAGAAGGAACCCAGTTGTTTCTTGCTCTCAAACGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

438

Amino Acids

49.01

Weight (kDa)

6.21

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 173
Acc65I GGTACC 1 cut(s) 703
AccB1I GGYRCC 3 cut(s) 58, 244, 703
AccI GTMKAC 4 cut(s) 340, 720, 950, 1094
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 2 cut(s) 56, 62
AcoI YGGCCR 2 cut(s) 552, 634
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 491, 705
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 6 cut(s) 83, 220, 333, 1009, 1075, 1180
AjnI CCWGG 1 cut(s) 1135
Ama87I CYCGRG 1 cut(s) 837
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 3 cut(s) 552, 634, 1133
ApeKI GCWGC 8 cut(s) 317, 320, 608, 683, 797, 821, 1150, 1269
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
Asp700I GAANNNNTTC 1 cut(s) 1248
Asp718I GGTACC 1 cut(s) 703
AspLEI GCGC 3 cut(s) 61, 134, 281
AspS9I GGNCC 5 cut(s) 90, 437, 900, 1133, 1207
AsuC2I CCSGG 2 cut(s) 31, 621
AsuHPI GGTGA 2 cut(s) 883, 1063
AvaI CYCGRG 1 cut(s) 837
AvaII GGWCC 4 cut(s) 90, 437, 900, 1207
BaeI ACNNNNGTAYC 2 cut(s) 473, 506
BalI TGGCCA 1 cut(s) 554
BanI GGYRCC 3 cut(s) 58, 244, 703
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbvI GCAGC 8 cut(s) 304, 307, 595, 695, 809, 833, 1162, 1256
BccI CCATC 4 cut(s) 269, 862, 916, 1039
BceAI ACGGC 1 cut(s) 649
BciT130I CCWGG 1 cut(s) 1137
BclI TGATCA 1 cut(s) 1125
BcnI CCSGG 2 cut(s) 31, 621
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 173
Bme1390I CCNGG 3 cut(s) 31, 621, 1137
Bme18I GGWCC 4 cut(s) 90, 437, 900, 1207
BmeT110I CYCGRG 1 cut(s) 837
BmgT120I GGNCC 5 cut(s) 90, 437, 900, 1133, 1207
BmiI GGNNCC 8 cut(s) 60, 92, 238, 246, 705, 901, 902, 1282
BmrFI CCNGG 3 cut(s) 31, 621, 1137
BmrI ACTGGG 1 cut(s) 1279
BmsI GCATC 7 cut(s) 16, 237, 340, 513, 778, 936, 1058
BmuI ACTGGG 1 cut(s) 1279
BplI GAGNNNNNCTC 2 cut(s) 986, 1018
BpuMI CCSGG 2 cut(s) 31, 621
BsaBI GATNNNNATC 2 cut(s) 151, 227
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 6 cut(s) 30, 240, 620, 631, 969, 1103
BsaWI WCCGGW 2 cut(s) 589, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 4 cut(s) 92, 637, 695, 1285
Bse8I GATNNNNATC 2 cut(s) 151, 227
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 1 cut(s) 1137
BseDI CCNNGG 6 cut(s) 30, 240, 620, 631, 969, 1103
BseGI GGATG 2 cut(s) 431, 1073
BseJI GATNNNNATC 2 cut(s) 151, 227
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 2 cut(s) 926, 1168
BseNI ACTGG 4 cut(s) 92, 637, 695, 1285
BseRI GAGGAG 1 cut(s) 499
BseXI GCAGC 8 cut(s) 304, 307, 595, 695, 809, 833, 1162, 1256
BsgI GTGCAG 1 cut(s) 151
BshFI GGCC 3 cut(s) 554, 636, 1135
BshNI GGYRCC 3 cut(s) 58, 244, 703
BsiHKCI CYCGRG 1 cut(s) 837
BsiSI CCGG 7 cut(s) 30, 248, 590, 620, 707, 734, 1116
BslFI GGGAC 4 cut(s) 35, 76, 126, 913
BslI CCNNNNNNNGG 1 cut(s) 266
BsmFI GGGAC 4 cut(s) 35, 76, 126, 913
BsmI GAATGC 2 cut(s) 670, 824
BsnI GGCC 3 cut(s) 554, 636, 1135
BsoBI CYCGRG 1 cut(s) 837
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 4 cut(s) 101, 544, 1053, 1125
Bsp19I CCATGG 1 cut(s) 1103
BspACI CCGC 2 cut(s) 56, 62
BspANI GGCC 3 cut(s) 554, 636, 1135
BspCNI CTCAG 2 cut(s) 925, 1167
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 8 cut(s) 60, 92, 238, 246, 705, 901, 902, 1282
BspMI ACCTGC 1 cut(s) 173
BspT107I GGYRCC 3 cut(s) 58, 244, 703
BsrFI RCCGGY 1 cut(s) 1115
BsrI ACTGG 4 cut(s) 92, 637, 695, 1285
BssAI RCCGGY 1 cut(s) 1115
BssECI CCNNGG 6 cut(s) 30, 240, 620, 631, 969, 1103
BssMI GATC 4 cut(s) 101, 544, 1053, 1125
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 969, 1103
Bst2UI CCWGG 1 cut(s) 1137
Bst4CI ACNGT 2 cut(s) 244, 269
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 613, 617, 885, 1162
BstDEI CTNAG 4 cut(s) 699, 912, 1154, 1197
BstDSI CCRYGG 3 cut(s) 240, 631, 1103
BstF5I GGATG 2 cut(s) 431, 1073
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 3 cut(s) 61, 134, 281
BstKTI GATC 4 cut(s) 104, 547, 1056, 1128
BstMBI GATC 4 cut(s) 101, 544, 1053, 1125
BstMWI GCNNNNNNNGC 3 cut(s) 67, 285, 797
BstNI CCWGG 1 cut(s) 1137
BstSCI CCNGG 3 cut(s) 29, 619, 1135
BstV1I GCAGC 8 cut(s) 304, 307, 595, 695, 809, 833, 1162, 1256
BsuRI GGCC 3 cut(s) 554, 636, 1135
BtgI CCRYGG 3 cut(s) 240, 631, 1103
BtsCI GGATG 2 cut(s) 431, 1073
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 3 cut(s) 155, 483, 688
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 4 cut(s) 613, 617, 885, 1162
CfoI GCGC 3 cut(s) 61, 134, 281
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 5 cut(s) 90, 437, 900, 1133, 1207
CseI GACGC 2 cut(s) 445, 567
Csp6I GTAC 2 cut(s) 490, 704
CviAII CATG 8 cut(s) 74, 548, 817, 880, 931, 958, 1104, 1165
CviQI GTAC 2 cut(s) 490, 704
DdeI CTNAG 4 cut(s) 699, 912, 1154, 1197
DinI GGCGCC 1 cut(s) 60
DpnI GATC 4 cut(s) 103, 546, 1055, 1127
DpnII GATC 4 cut(s) 101, 544, 1053, 1125
EaeI YGGCCR 2 cut(s) 552, 634
Eco130I CCWWGG 2 cut(s) 969, 1103
Eco32I GATATC 1 cut(s) 149
Eco47I GGWCC 4 cut(s) 90, 437, 900, 1207
Eco57I CTGAAG 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 837
EcoO109I RGGNCCY 1 cut(s) 900
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 1135
EcoRV GATATC 1 cut(s) 149
EcoT14I CCWWGG 2 cut(s) 969, 1103
EcoT22I ATGCAT 1 cut(s) 506
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 969, 1103
FaeI CATG 8 cut(s) 77, 551, 820, 883, 934, 961, 1107, 1168
FaqI GGGAC 4 cut(s) 35, 76, 126, 913
FatI CATG 8 cut(s) 73, 547, 816, 879, 930, 957, 1103, 1164
FauNDI CATATG 1 cut(s) 1122
FbaI TGATCA 1 cut(s) 1125
FblI GTMKAC 4 cut(s) 340, 720, 950, 1094
FokI GGATG 2 cut(s) 438, 1080
GlaI GCGC 3 cut(s) 60, 133, 280
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 3 cut(s) 554, 636, 1135
HapII CCGG 7 cut(s) 30, 248, 590, 620, 707, 734, 1116
HgaI GACGC 2 cut(s) 445, 567
HhaI GCGC 3 cut(s) 61, 134, 281
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 8 cut(s) 77, 551, 820, 883, 934, 961, 1107, 1168
Hin6I GCGC 3 cut(s) 59, 132, 279
HinP1I GCGC 3 cut(s) 59, 132, 279
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 68, 365
HinfI GANTC 5 cut(s) 337, 916, 1001, 1091, 1140
HpaII CCGG 7 cut(s) 30, 248, 590, 620, 707, 734, 1116
HphI GGTGA 2 cut(s) 883, 1063
Hpy166II GTNNAC 5 cut(s) 341, 721, 951, 1000, 1095
Hpy188I TCNGA 5 cut(s) 261, 411, 915, 1035, 1157
Hpy188III TCNNGA 5 cut(s) 93, 176, 310, 470, 590
Hpy8I GTNNAC 5 cut(s) 341, 721, 951, 1000, 1095
HpyAV CCTTC 4 cut(s) 968, 983, 1030, 1271
HpyCH4III ACNGT 2 cut(s) 244, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 285, 797
HpyF3I CTNAG 4 cut(s) 699, 912, 1154, 1197
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 8 cut(s) 77, 551, 820, 883, 934, 961, 1107, 1168
HspAI GCGC 3 cut(s) 59, 132, 279
KasI GGCGCC 1 cut(s) 58
KflI GGGWCCC 1 cut(s) 900
Kpn2I TCCGGA 1 cut(s) 589
KpnI GGTACC 1 cut(s) 707
Ksp22I TGATCA 1 cut(s) 1125
Kzo9I GATC 4 cut(s) 101, 544, 1053, 1125
LmnI GCTCC 2 cut(s) 663, 709
Lsp1109I GCAGC 8 cut(s) 304, 307, 595, 695, 809, 833, 1162, 1256
LweI GCATC 7 cut(s) 16, 237, 340, 513, 778, 936, 1058
MalI GATC 4 cut(s) 103, 546, 1055, 1127
MboI GATC 4 cut(s) 101, 544, 1053, 1125
MboII GAAGA 5 cut(s) 232, 529, 666, 763, 1253
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 5 cut(s) 139, 171, 524, 803, 1086
MluNI TGGCCA 1 cut(s) 554
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 4 cut(s) 346, 995, 1100, 1134
MmeI TCCRAC 3 cut(s) 415, 419, 1040
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 506
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 554
MseI TTAA 2 cut(s) 564, 1109
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 1269
MspI CCGG 7 cut(s) 30, 248, 590, 620, 707, 734, 1116
MspR9I CCNGG 3 cut(s) 31, 621, 1137
Mva1269I GAATGC 2 cut(s) 670, 824
MvaI CCWGG 1 cut(s) 1137
MwoI GCNNNNNNNGC 3 cut(s) 67, 285, 797
NarI GGCGCC 1 cut(s) 59
NciI CCSGG 2 cut(s) 31, 621
NcoI CCATGG 1 cut(s) 1103
NdeI CATATG 1 cut(s) 1122
NdeII GATC 4 cut(s) 101, 544, 1053, 1125
NlaIII CATG 8 cut(s) 77, 551, 820, 883, 934, 961, 1107, 1168
NlaIV GGNNCC 8 cut(s) 60, 92, 238, 246, 705, 901, 902, 1282
NsiI ATGCAT 1 cut(s) 506
PctI GAATGC 2 cut(s) 670, 824
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 1 cut(s) 916
PleI GAGTC 4 cut(s) 345, 995, 1099, 1134
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 4 cut(s) 345, 995, 1099, 1134
PpuMI RGGWCCY 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 900
Psp6I CCWGG 1 cut(s) 1135
PspGI CCWGG 1 cut(s) 1135
PspN4I GGNNCC 8 cut(s) 60, 92, 238, 246, 705, 901, 902, 1282
PspPI GGNCC 5 cut(s) 90, 437, 900, 1133, 1207
PspPPI RGGWCCY 1 cut(s) 900
PvuII CAGCTG 1 cut(s) 1269
RsaI GTAC 2 cut(s) 491, 705
RsaNI GTAC 2 cut(s) 490, 704
SalI GTCGAC 1 cut(s) 1093
SaqAI TTAA 2 cut(s) 564, 1109
Sau3AI GATC 4 cut(s) 101, 544, 1053, 1125
Sau96I GGNCC 5 cut(s) 90, 437, 900, 1133, 1207
SchI GAGTC 4 cut(s) 346, 995, 1100, 1134
ScrFI CCNGG 3 cut(s) 31, 621, 1137
SfaNI GCATC 7 cut(s) 16, 237, 340, 513, 778, 936, 1058
SfoI GGCGCC 1 cut(s) 60
SinI GGWCC 4 cut(s) 90, 437, 900, 1207
Sse9I AATT 5 cut(s) 139, 171, 524, 803, 1086
SsiI CCGC 2 cut(s) 56, 62
SspDI GGCGCC 1 cut(s) 58
StyD4I CCNGG 3 cut(s) 29, 619, 1135
StyI CCWWGG 2 cut(s) 969, 1103
TaaI ACNGT 2 cut(s) 244, 269
TaqI TCGA 3 cut(s) 51, 1056, 1094
TasI AATT 5 cut(s) 139, 171, 524, 803, 1086
TauI GCSGC 2 cut(s) 59, 64
TfiI GAWTC 1 cut(s) 916
Tru1I TTAA 2 cut(s) 564, 1109
Tru9I TTAA 2 cut(s) 564, 1109
TscAI CASTG 3 cut(s) 162, 483, 695
TseI GCWGC 8 cut(s) 317, 320, 608, 683, 797, 821, 1150, 1269
TspDTI ATGAA 5 cut(s) 62, 278, 792, 1245, 1254
TspGWI ACGGA 2 cut(s) 249, 1220
TspRI CASTG 3 cut(s) 162, 483, 695
VpaK11BI GGWCC 4 cut(s) 90, 437, 900, 1207
XapI RAATTY 1 cut(s) 524
XmiI GTMKAC 4 cut(s) 340, 720, 950, 1094
XmnI GAANNNNTTC 1 cut(s) 1248
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.