Rw4G037030

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018707
Physical Location & Seq
Reverse (-)
63765985 .. 63767588
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037030.1

Sequence Viewer

Length: 960 bp
ATGAAGCTTGACATAAACGAGATTACTTCTTCGACCAATGCATTTTGTATAGCAGATTTGGGGTGTTCAGTTGGACCAAACTGTTTTGTTTCTGTGGAGAACATACTGGAAGCTATTAAACATAAGTACCAGTCCGGATGCATGTCTTCCCAAATGCCTGAATTCCAAGTCTTCTTTAATGATCATGCGGGCAATGATTTCAATACCCTATTTGCAAATTTCCCTCCGACAAGGCACTACTTTGCAGCTGGTGTGCCGGGTTCTTTCCACGGCCGATTATTCCCCAAGTCCTCTCTCCATTTCGTGCATTCTTCATATGCAACGCACTGGCTCTCAAAGGTACCAGAAGAACTGACAGACAAGAACTCTCCGGCATGGAACAAAGGGAAGATTCACTACACAACTGCCCCTGAAGAAGTAGTTAATGCTTATGCAGCTCAATTTGCCAAGGACATGGCAACATTCTTGGAAGCTCGCGCTCAAGAGCTTGTGGTTGGTGGAATGATGATGCTAATCATGCCAGCTGTCCCAACTGGGATCCCTCATTCCCGTGTTCCAAATGGAGTGATGTTTGATTTTCTGGGGTCTATTCTCATGGATATGGCAAAGGAAGGAGTTATATCTGAAGCTGAGGTGGACTCATTCAACTTGCCAGTGTACACTACCTCTCCCAAGGAGATGGGAGAGCTGGTAGAGAGAAATGGATGCTTTAGCATAGAGCGAATGGAGTCAACGAGCCCCTGGCTTGCTTCCAAGCATTCGAACTCCAACTACGGGAAGACACTGTCGGTGGCGCTAAGAGCTGGCATGGAGGGAGTTTTCAAAAGCCATTTTGGAAGTGAGATTACCAATCAACTGTTTAATAGGCTCTGTGACCAAAGTGGACAACTTACCCACCAACTAGGGGAGTCCTGCTGCAGAGAAGGAACTCAGTTGTTTGTTGCTCTCAAGCGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

319

Amino Acids

35.26

Weight (kDa)

6.06

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 10 - 318 8.8e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 340
AccII CGCG 1 cut(s) 477
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 1 cut(s) 188
AclWI GGATC 2 cut(s) 532, 545
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 161, 217
AcuI CTGAAG 2 cut(s) 432, 645
AfaI GTAC 3 cut(s) 128, 342, 659
AfiI CCNNNNNNNGG 2 cut(s) 774, 904
AgsI TTSAA 3 cut(s) 202, 646, 823
AjnI CCWGG 1 cut(s) 740
AlwI GGATC 2 cut(s) 532, 545
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 3 cut(s) 245, 434, 915
ApoI RAATTY 2 cut(s) 161, 217
Asp718I GGTACC 1 cut(s) 340
AspLEI GCGC 3 cut(s) 479, 796, 954
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 258
AsuII TTCGAA 1 cut(s) 761
AvaII GGWCC 1 cut(s) 74
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BamHI GGATCC 1 cut(s) 537
BanI GGYRCC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 740
BarI GAAGNNNNNNTAC 4 cut(s) 380, 412, 829, 861
BbsI GAAGAC 3 cut(s) 138, 163, 785
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 3 cut(s) 257, 446, 902
BccI CCATC 1 cut(s) 673
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 742
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 258
BfaI CTAG 1 cut(s) 902
BfmI CTRYAG 1 cut(s) 916
BfoI RGCGCY 1 cut(s) 797
BisI GCNGC 3 cut(s) 246, 435, 916
BlsI GCNGC 3 cut(s) 247, 436, 917
Bme1390I CCNGG 2 cut(s) 258, 742
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 342, 539
BmrFI CCNGG 2 cut(s) 258, 742
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 3 cut(s) 128, 498, 695
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 138, 163, 785
BplI GAGNNNNNCTC 2 cut(s) 623, 655
Bpu10I CCTNAGC 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 761
BpuEI CTTGAG 2 cut(s) 465, 932
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 268, 447, 672, 740
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 4 cut(s) 652, 675, 682, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 774, 904
Bse1I ACTGG 5 cut(s) 111, 130, 332, 538, 653
Bse3DI GCAATG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 512
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 1 cut(s) 742
BseDI CCNNGG 4 cut(s) 268, 447, 672, 740
BseGI GGATG 2 cut(s) 143, 710
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 2 cut(s) 774, 904
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 621, 944
BseNI ACTGG 5 cut(s) 111, 130, 332, 538, 653
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 257, 446, 902
Bsh1236I CGCG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 274
BsiSI CCGG 3 cut(s) 135, 257, 371
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 2 cut(s) 774, 904
BsmFI GGGAC 1 cut(s) 512
BsmI GAATGC 2 cut(s) 307, 757
BsnI GGCC 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 761
Bsp1286I GDGCHC 1 cut(s) 740
Bsp13I TCCGGA 1 cut(s) 134
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 2 cut(s) 181, 537
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 622, 943
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 342, 539
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 2 cut(s) 532, 545
BspT104I TTCGAA 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 199
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 5 cut(s) 111, 130, 332, 538, 653
BssECI CCNNGG 4 cut(s) 268, 447, 672, 740
BssMI GATC 2 cut(s) 181, 537
BssT1I CCWWGG 2 cut(s) 447, 672
Bst2UI CCWGG 1 cut(s) 742
Bst4CI ACNGT 3 cut(s) 83, 786, 858
BstAUI TGTACA 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 761
BstC8I GCNNGC 5 cut(s) 190, 475, 522, 747, 805
BstDEI CTNAG 3 cut(s) 630, 797, 930
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 143, 710
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 797
BstHHI GCGC 3 cut(s) 479, 796, 954
BstKTI GATC 2 cut(s) 184, 540
BstMBI GATC 2 cut(s) 181, 537
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 4 cut(s) 434, 443, 517, 800
BstNI CCWGG 1 cut(s) 742
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 256, 740
BstSFI CTRYAG 1 cut(s) 916
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 3 cut(s) 257, 446, 902
BstV2I GAAGAC 3 cut(s) 138, 163, 785
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 2 cut(s) 454, 679
BstYI RGATCY 1 cut(s) 537
BstZI CGGCCG 1 cut(s) 271
BsuRI GGCC 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 2 cut(s) 143, 710
BtsIMutI CAGTG 3 cut(s) 325, 660, 782
Cac8I GCNNGC 5 cut(s) 190, 475, 522, 747, 805
CfoI GCGC 3 cut(s) 479, 796, 954
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 3 cut(s) 127, 341, 658
CviAII CATG 7 cut(s) 142, 185, 375, 454, 517, 595, 808
CviQI GTAC 3 cut(s) 127, 341, 658
DdeI CTNAG 3 cut(s) 630, 797, 930
DpnI GATC 2 cut(s) 183, 539
DpnII GATC 2 cut(s) 181, 537
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 447, 672
Eco24I GRGCYC 1 cut(s) 740
Eco47I GGWCC 1 cut(s) 74
Eco52I CGGCCG 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 432, 645
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 740
EcoT14I CCWWGG 2 cut(s) 447, 672
EcoT22I ATGCAT 2 cut(s) 43, 143
EcoT38I GRGCYC 1 cut(s) 740
ErhI CCWWGG 2 cut(s) 447, 672
FaeI CATG 7 cut(s) 145, 188, 378, 457, 520, 598, 811
FaqI GGGAC 1 cut(s) 512
FatI CATG 7 cut(s) 141, 184, 374, 453, 516, 594, 807
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 246, 435, 916
FokI GGATG 2 cut(s) 150, 717
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 3 cut(s) 246, 435, 916
FspBI CTAG 1 cut(s) 902
GlaI GCGC 3 cut(s) 478, 795, 953
GluI GCNGC 3 cut(s) 246, 435, 916
HaeII RGCGCY 1 cut(s) 797
HaeIII GGCC 1 cut(s) 273
HapII CCGG 3 cut(s) 135, 257, 371
HhaI GCGC 3 cut(s) 479, 796, 954
Hin1II CATG 7 cut(s) 145, 188, 378, 457, 520, 598, 811
Hin6I GCGC 3 cut(s) 477, 794, 952
HinP1I GCGC 3 cut(s) 477, 794, 952
HincII GTYRAC 1 cut(s) 732
HindII GTYRAC 1 cut(s) 732
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 4 cut(s) 391, 638, 728, 908
HpaII CCGG 3 cut(s) 135, 257, 371
Hpy166II GTNNAC 5 cut(s) 637, 658, 660, 732, 884
Hpy188I TCNGA 2 cut(s) 228, 625
Hpy188III TCNNGA 2 cut(s) 135, 482
Hpy8I GTNNAC 5 cut(s) 637, 658, 660, 732, 884
HpyAV CCTTC 2 cut(s) 605, 917
HpyCH4III ACNGT 3 cut(s) 83, 786, 858
HpyCH4V TGCA 8 cut(s) 41, 141, 215, 245, 307, 320, 434, 918
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 443, 517, 800
HpyF3I CTNAG 3 cut(s) 630, 797, 930
Hsp92II CATG 7 cut(s) 145, 188, 378, 457, 520, 598, 811
HspAI GCGC 3 cut(s) 477, 794, 952
Kpn2I TCCGGA 1 cut(s) 134
KpnI GGTACC 1 cut(s) 344
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 181, 537
Lsp1109I GCAGC 3 cut(s) 257, 446, 902
LweI GCATC 3 cut(s) 128, 498, 695
MaeI CTAG 1 cut(s) 902
MaeIII GTNAC 1 cut(s) 872
MalI GATC 2 cut(s) 183, 539
MboI GATC 2 cut(s) 181, 537
MboII GAAGA 8 cut(s) 21, 138, 163, 303, 359, 400, 425, 790
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 3 cut(s) 161, 217, 440
MlyI GAGTC 3 cut(s) 632, 737, 917
MmeI TCCRAC 3 cut(s) 52, 251, 792
MnlI CCTC 6 cut(s) 234, 301, 552, 625, 676, 805
Mph1103I ATGCAT 2 cut(s) 43, 143
MroI TCCGGA 1 cut(s) 134
MseI TTAA 4 cut(s) 117, 177, 423, 861
MslI CAYNNNNRTG 2 cut(s) 549, 599
MspA1I CMGCKG 2 cut(s) 248, 524
MspI CCGG 3 cut(s) 135, 257, 371
MspR9I CCNGG 2 cut(s) 258, 742
Mva1269I GAATGC 2 cut(s) 307, 757
MvaI CCWGG 1 cut(s) 742
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 4 cut(s) 434, 443, 517, 800
NciI CCSGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 316
NdeII GATC 2 cut(s) 181, 537
NlaIII CATG 7 cut(s) 145, 188, 378, 457, 520, 598, 811
NlaIV GGNNCC 2 cut(s) 342, 539
NmuCI GTSAC 1 cut(s) 872
NsiI ATGCAT 2 cut(s) 43, 143
NspI RCATGY 1 cut(s) 145
NspV TTCGAA 1 cut(s) 761
PctI GAATGC 2 cut(s) 307, 757
PfeI GAWTC 1 cut(s) 391
PflFI GACNNNGTC 1 cut(s) 784
PkrI GCNGC 3 cut(s) 247, 436, 917
PleI GAGTC 3 cut(s) 632, 736, 916
PpsI GAGTC 3 cut(s) 632, 736, 916
Psp6I CCWGG 1 cut(s) 740
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 2 cut(s) 342, 539
PspPI GGNCC 1 cut(s) 74
PsrI GAACNNNNNNTAC 2 cut(s) 755, 787
PstI CTGCAG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 537
PsyI GACNNNGTC 1 cut(s) 784
PvuII CAGCTG 2 cut(s) 248, 524
RsaI GTAC 3 cut(s) 128, 342, 659
RsaNI GTAC 3 cut(s) 127, 341, 658
RseI CAYNNNNRTG 2 cut(s) 549, 599
SaqAI TTAA 4 cut(s) 117, 177, 423, 861
SatI GCNGC 3 cut(s) 246, 435, 916
Sau3AI GATC 2 cut(s) 181, 537
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 3 cut(s) 632, 737, 917
ScrFI CCNGG 2 cut(s) 258, 742
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 3 cut(s) 128, 498, 695
SfcI CTRYAG 1 cut(s) 916
SfuI TTCGAA 1 cut(s) 761
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 549, 599
SmlI CTYRAG 2 cut(s) 480, 947
SmoI CTYRAG 2 cut(s) 480, 947
Sse9I AATT 3 cut(s) 161, 217, 440
SsiI CCGC 1 cut(s) 188
SspMI CTAG 1 cut(s) 902
StyD4I CCNGG 2 cut(s) 256, 740
StyI CCWWGG 2 cut(s) 447, 672
TaaI ACNGT 3 cut(s) 83, 786, 858
TaqI TCGA 2 cut(s) 32, 761
TasI AATT 3 cut(s) 161, 217, 440
TatI WGTACW 1 cut(s) 657
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 117, 177, 423, 861
Tru9I TTAA 4 cut(s) 117, 177, 423, 861
TscAI CASTG 3 cut(s) 332, 660, 789
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 3 cut(s) 245, 434, 915
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 2 cut(s) 17, 303
TspRI CASTG 3 cut(s) 332, 660, 789
Tth111I GACNNNGTC 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 2 cut(s) 161, 217
XceI RCATGY 1 cut(s) 145
XspI CTAG 1 cut(s) 902
Zsp2I ATGCAT 2 cut(s) 43, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.