Rmu_sc0005526.1_g000007

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005526.1
OG0018707
Physical Location & Seq
Forward (+)
49218 .. 49971
754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005526.1_g000007.1.cds

Sequence Viewer

Length: 609 bp
ctgacagacaagaactctccggcatggaacaaagggaagattcactacacaactgcccctgaagaagtagttaatgcttatgcagctcaatttgccaaggacatggcaacattcttggaagctcgcgctcaagagcttgtggttggtggaatgatgatgctaatcatgccagctgtcccaactgggatccctcattcccgtgttccaaatggagtgatgtttgattttctggggtctattctcaaggatatggcaaaggaaggagttatatctgaagctgaggtggactcattcaacttgccagtgtacactacctctcccaaggagatgggagagctggtagagagaaatggatgctttagcatagagcgaatggagtcaacgagcccctggcttgtttccaagcattcgaactccaactacgggaagacactgtcgatggggctaagagctggcatggagggagttttcaaaagccattttggaagtgagattaccaatcaactgttcaataggctctgtgaccaaagtggacaacttacccaacaactaggggagtcctgctgcagagaaggaactcagttgtttgttgctctcaagcgcaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

202

Amino Acids

22.27

Weight (kDa)

6.13

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 126
AclWI GGATC 2 cut(s) 181, 194
AcuI CTGAAG 2 cut(s) 81, 294
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 3 cut(s) 295, 472, 511
AjnI CCWGG 1 cut(s) 389
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 7 cut(s) 86, 122, 136, 173, 278, 337, 452
AluI AGCT 7 cut(s) 86, 122, 136, 173, 278, 337, 452
AlwI GGATC 2 cut(s) 181, 194
ApeKI GCWGC 2 cut(s) 83, 564
AspLEI GCGC 2 cut(s) 128, 603
AsuII TTCGAA 1 cut(s) 410
BamHI GGATCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 389
BarI GAAGNNNNNNTAC 4 cut(s) 29, 61, 478, 510
BbsI GAAGAC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 95, 551
BccI CCATC 2 cut(s) 322, 433
BciT130I CCWGG 1 cut(s) 391
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 565
BisI GCNGC 2 cut(s) 84, 565
BlsI GCNGC 2 cut(s) 85, 566
Bme1390I CCNGG 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 1 cut(s) 391
BmrI ACTGGG 1 cut(s) 192
BmsI GCATC 2 cut(s) 147, 344
BmuI ACTGGG 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 434
BplI GAGNNNNNCTC 2 cut(s) 272, 304
Bpu10I CCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 3 cut(s) 114, 227, 581
BsaBI GATNNNNATC 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 96, 321, 389
BsaXI ACNNNNNCTCC 4 cut(s) 301, 324, 331, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse1I ACTGG 2 cut(s) 187, 302
Bse8I GATNNNNATC 1 cut(s) 161
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 3 cut(s) 96, 321, 389
BseGI GGATG 1 cut(s) 359
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 2 cut(s) 270, 593
BseNI ACTGG 2 cut(s) 187, 302
BseXI GCAGC 2 cut(s) 95, 551
Bsh1236I CGCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 389
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 1 cut(s) 186
BspCNI CTCAG 2 cut(s) 271, 592
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 1 cut(s) 569
BspPI GGATC 2 cut(s) 181, 194
BspT104I TTCGAA 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 2 cut(s) 187, 302
BssECI CCNNGG 3 cut(s) 96, 321, 389
BssMI GATC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 96, 321
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 435, 507
BstAUI TGTACA 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 124, 171, 454
BstDEI CTNAG 3 cut(s) 279, 446, 579
BstF5I GGATG 1 cut(s) 359
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 2 cut(s) 128, 603
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 83, 92, 166
BstNI CCWGG 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 389
BstSFI CTRYAG 1 cut(s) 565
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 95, 551
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 186
BstXI CCANNNNNNTGG 2 cut(s) 103, 328
BstYI RGATCY 1 cut(s) 186
BtsCI GGATG 1 cut(s) 359
BtsIMutI CAGTG 2 cut(s) 309, 431
Cac8I GCNNGC 3 cut(s) 124, 171, 454
CfoI GCGC 2 cut(s) 128, 603
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 24, 103, 166, 457
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 3 cut(s) 279, 446, 579
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 96, 321
Eco24I GRGCYC 1 cut(s) 389
Eco57I CTGAAG 2 cut(s) 81, 294
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 2 cut(s) 96, 321
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 2 cut(s) 96, 321
FaeI CATG 4 cut(s) 27, 106, 169, 460
FaiI YATR 8 cut(s) 25, 81, 104, 167, 251, 269, 365, 458
FaqI GGGAC 1 cut(s) 161
FatI CATG 4 cut(s) 23, 102, 165, 456
Fnu4HI GCNGC 2 cut(s) 84, 565
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 84, 565
FspBI CTAG 1 cut(s) 551
GlaI GCGC 2 cut(s) 127, 602
GluI GCNGC 2 cut(s) 84, 565
HapII CCGG 1 cut(s) 20
HhaI GCGC 2 cut(s) 128, 603
Hin1II CATG 4 cut(s) 27, 106, 169, 460
Hin6I GCGC 2 cut(s) 126, 601
HinP1I GCGC 2 cut(s) 126, 601
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 4 cut(s) 40, 287, 377, 557
HpaII CCGG 1 cut(s) 20
Hpy166II GTNNAC 5 cut(s) 286, 307, 309, 381, 533
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 5 cut(s) 286, 307, 309, 381, 533
HpyAV CCTTC 2 cut(s) 254, 566
HpyCH4III ACNGT 2 cut(s) 435, 507
HpyCH4V TGCA 2 cut(s) 83, 567
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 92, 166
HpyF3I CTNAG 3 cut(s) 279, 446, 579
Hsp92II CATG 4 cut(s) 27, 106, 169, 460
HspAI GCGC 2 cut(s) 126, 601
Kzo9I GATC 1 cut(s) 186
Lsp1109I GCAGC 2 cut(s) 95, 551
LweI GCATC 2 cut(s) 147, 344
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 1 cut(s) 521
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 49, 74, 439
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 1 cut(s) 89
MlyI GAGTC 3 cut(s) 281, 386, 566
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 4 cut(s) 201, 274, 325, 454
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 391
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 3 cut(s) 83, 92, 166
NdeII GATC 1 cut(s) 186
NlaIII CATG 4 cut(s) 27, 106, 169, 460
NlaIV GGNNCC 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 521
NspV TTCGAA 1 cut(s) 410
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 40
PflFI GACNNNGTC 1 cut(s) 433
PkrI GCNGC 2 cut(s) 85, 566
PleI GAGTC 3 cut(s) 281, 385, 565
PpsI GAGTC 3 cut(s) 281, 385, 565
Psp6I CCWGG 1 cut(s) 389
PspGI CCWGG 1 cut(s) 389
PspN4I GGNNCC 1 cut(s) 188
PsrI GAACNNNNNNTAC 2 cut(s) 404, 436
PstI CTGCAG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 186
PsyI GACNNNGTC 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 2 cut(s) 84, 565
Sau3AI GATC 1 cut(s) 186
SchI GAGTC 3 cut(s) 281, 386, 566
ScrFI CCNGG 1 cut(s) 391
SduI GDGCHC 1 cut(s) 389
SetI ASST 9 cut(s) 88, 124, 138, 175, 280, 285, 317, 339, 454
SfaNI GCATC 2 cut(s) 147, 344
SfcI CTRYAG 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 3 cut(s) 129, 242, 596
SmoI CTYRAG 3 cut(s) 129, 242, 596
Sse9I AATT 1 cut(s) 89
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 389
StyI CCWWGG 2 cut(s) 96, 321
TaaI ACNGT 2 cut(s) 435, 507
TaqI TCGA 2 cut(s) 410, 437
TasI AATT 1 cut(s) 89
TatI WGTACW 1 cut(s) 306
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 2 cut(s) 309, 438
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 2 cut(s) 83, 564
Tsp45I GTSAC 1 cut(s) 521
TspRI CASTG 2 cut(s) 309, 438
Tth111I GACNNNGTC 1 cut(s) 433
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.