MD13G1007200.v1.1

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0018707
Physical Location & Seq
Forward (+)
466657 .. 468452
1796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1007200.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGAGCAGCAACGAAACCACAGTGCCGGAAGCCTATCCGATGAACGGTGGAGATGGCGCATACAGCTACACCAAAAACTCCAATTGCCAGAGAGCTGCAGCAAATGTTTCAAAGAGTCTACTTGATGATGCAATAGCAGAGAAGCTTGACGTAGAGGACTTCTCTTGCAATCCAAGCAACGCATTTCGGATAGCAGATTTGGGATGTTCAGTTGGACCAAACACTTTCTTTAGTGTCCAAAACATTCTGGAGGCAGTGAACCACAAGTACCAATCCCAATGCATCTCCTCCCAAATGCCTGAATTCCAAGTATTCTTCAGTGATCATGTTGCCAACGATTTCAACACCCTTTTCGCATCTTTGCCTCCGGAAAGGCAGTACTTTGCAGCTGGCGTGCCGGGATCTTTCCACGGCCAGTTATTCCCCAAGTCCTCGCTTCATTTCGTGCATTCTTCGTATGCAGCTCACTGGCTCTCTAAGGTACCGGAGCAAGTGGTAGACAAGAACTCTCCGGCGTGGAACAAAGGGAAGATTTACTACACGACCTCCCCTGATGAAGTAGTTGATGCTTATGCAGCCCAATTTGGTAAAGACATGACAGCATTCTTGGAAGCTAGGGCTCAAGAGCTTGTGGTGGGTGGAATGATGGTGATAATCATGCAAGCCATCCCAAACGGGACCCCTCCATCTCGGATTCCAAATGGCATCATGTTTGATTTTCTGGGGTCTACTCTCATGGAGATTGCCAAGGAAGGATTGATTAGCGAAGGAGAGGTGGACTCGTTCAACATTCCAACATACAACACAACTCCGAAGGAAATGATGGAGGTGATCGAAAGAAATGGATGCTTCAGCATAGCGAGAATTGAGTCGACAAGCCCATGGTCAAAAGCCGGTCATATGATCAACGGGCCGGGACTCACAATGCAGCTCAGAGCTGGCATGGAGGGAGTTTTCAAAACACATTTTGGAACTGAGATAACGGACCAAATGTTTGATAGGGTTTATGAAAAAAGTGGAGAACTTATTCACAAAATAGAATCCAGCTGCAAAGAAGGAACTCAGTTGTTTCTTGCTCTCAAACGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

364

Amino Acids

40.04

Weight (kDa)

5.25

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 57 - 362 1.6e-104 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36770
malus_domestica MD13G1007200.v1.1
prunus_persica Prupe.1G345700_v2.0.a1
pyrus_communis pycom13g00610
rosa_chinensis RchiOBHm_Chr4g0446481
rosa_multiflora Rmu_sc0005526.1_g000007
rosa_rugosa Rorug04G0371800
rosa_samantha Rh4CG459900
rosa_wichuraiana Rw4G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 481
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 4 cut(s) 118, 498, 728, 872
AccIII TCCGGA 1 cut(s) 367
AclWI GGATC 1 cut(s) 409
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 2 cut(s) 301, 835
AfaI GTAC 3 cut(s) 269, 380, 483
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 4 cut(s) 111, 343, 787, 958
AlwI GGATC 1 cut(s) 409
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 2 cut(s) 412, 911
ApeKI GCWGC 8 cut(s) 6, 95, 98, 386, 461, 575, 928, 1047
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 977, 1009
Asp700I GAANNNNTTC 1 cut(s) 1026
Asp718I GGTACC 1 cut(s) 481
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 215, 678, 911, 985
AsuC2I CCSGG 2 cut(s) 399, 915
AsuHPI GGTGA 2 cut(s) 661, 841
AvaII GGWCC 3 cut(s) 215, 678, 985
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 622
BarI GAAGNNNNNNTAC 2 cut(s) 521, 553
BbvI GCAGC 8 cut(s) 18, 82, 110, 398, 473, 587, 940, 1034
BccI CCATC 5 cut(s) 47, 640, 674, 694, 817
BceAI ACGGC 1 cut(s) 427
BclI TGATCA 2 cut(s) 322, 903
BcnI CCSGG 2 cut(s) 399, 915
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 8 cut(s) 7, 96, 99, 387, 462, 576, 929, 1048
BlsI GCNGC 8 cut(s) 8, 97, 100, 388, 463, 577, 930, 1049
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 2 cut(s) 399, 915
Bme18I GGWCC 3 cut(s) 215, 678, 985
BmgT120I GGNCC 4 cut(s) 215, 678, 911, 985
BmiI GGNNCC 3 cut(s) 483, 679, 680
BmrFI CCNGG 2 cut(s) 399, 915
BmsI GCATC 6 cut(s) 118, 291, 365, 556, 714, 836
BplI GAGNNNNNCTC 4 cut(s) 146, 178, 764, 796
BpmI CTGGAG 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 606
BpuMI CCSGG 2 cut(s) 399, 915
BsaJI CCNNGG 3 cut(s) 409, 747, 881
BsaWI WCCGGW 2 cut(s) 367, 484
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 893
Bse1I ACTGG 2 cut(s) 415, 473
BseAI TCCGGA 1 cut(s) 367
BseDI CCNNGG 3 cut(s) 409, 747, 881
BseGI GGATG 3 cut(s) 209, 666, 851
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 3 cut(s) 946, 966, 1076
BseNI ACTGG 2 cut(s) 415, 473
BseRI GAGGAG 1 cut(s) 277
BseXI GCAGC 8 cut(s) 18, 82, 110, 398, 473, 587, 940, 1034
BshFI GGCC 2 cut(s) 414, 913
BshNI GGYRCC 1 cut(s) 481
BsiSI CCGG 7 cut(s) 26, 368, 398, 485, 512, 894, 914
BslFI GGGAC 2 cut(s) 691, 930
BslI CCNNNNNNNGG 1 cut(s) 44
BsmFI GGGAC 2 cut(s) 691, 930
BsmI GAATGC 2 cut(s) 448, 602
BsnI GGCC 2 cut(s) 414, 913
Bsp1286I GDGCHC 1 cut(s) 622
Bsp13I TCCGGA 1 cut(s) 367
Bsp143I GATC 4 cut(s) 322, 401, 831, 903
Bsp19I CCATGG 1 cut(s) 881
BspANI GGCC 2 cut(s) 414, 913
BspCNI CTCAG 3 cut(s) 945, 967, 1075
BspEI TCCGGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 483, 679, 680
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 481
BsrFI RCCGGY 1 cut(s) 893
BsrI ACTGG 2 cut(s) 415, 473
BssAI RCCGGY 1 cut(s) 893
BssECI CCNNGG 3 cut(s) 409, 747, 881
BssMI GATC 4 cut(s) 322, 401, 831, 903
BssT1I CCWWGG 2 cut(s) 747, 881
Bst4CI ACNGT 2 cut(s) 22, 47
BstC8I GCNNGC 4 cut(s) 391, 395, 663, 940
BstDEI CTNAG 4 cut(s) 477, 932, 975, 1062
BstDSI CCRYGG 2 cut(s) 409, 881
BstF5I GGATG 3 cut(s) 209, 666, 851
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 4 cut(s) 325, 404, 834, 906
BstMBI GATC 4 cut(s) 322, 401, 831, 903
BstMWI GCNNNNNNNGC 3 cut(s) 63, 174, 575
BstSCI CCNGG 2 cut(s) 397, 913
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 8 cut(s) 18, 82, 110, 398, 473, 587, 940, 1034
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BsuRI GGCC 2 cut(s) 414, 913
BtgI CCRYGG 2 cut(s) 409, 881
BtsCI GGATG 3 cut(s) 209, 666, 851
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 4 cut(s) 27, 261, 325, 466
Cac8I GCNNGC 4 cut(s) 391, 395, 663, 940
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 893
Cfr13I GGNCC 4 cut(s) 215, 678, 911, 985
Csp6I GTAC 3 cut(s) 268, 379, 482
CviAII CATG 7 cut(s) 326, 595, 658, 709, 736, 882, 943
CviQI GTAC 3 cut(s) 268, 379, 482
DdeI CTNAG 4 cut(s) 477, 932, 975, 1062
DpnI GATC 4 cut(s) 324, 403, 833, 905
DpnII GATC 4 cut(s) 322, 401, 831, 903
EaeI YGGCCR 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 747, 881
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 3 cut(s) 215, 678, 985
Eco57I CTGAAG 2 cut(s) 301, 835
EcoO109I RGGNCCY 1 cut(s) 678
EcoRI GAATTC 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 747, 881
EcoT22I ATGCAT 1 cut(s) 284
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 2 cut(s) 747, 881
FaeI CATG 7 cut(s) 329, 598, 661, 712, 739, 885, 946
FaqI GGGAC 2 cut(s) 691, 930
FatI CATG 7 cut(s) 325, 594, 657, 708, 735, 881, 942
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 2 cut(s) 322, 903
FblI GTMKAC 4 cut(s) 118, 498, 728, 872
Fnu4HI GCNGC 8 cut(s) 7, 96, 99, 387, 462, 576, 929, 1048
FokI GGATG 3 cut(s) 216, 653, 858
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 8 cut(s) 7, 96, 99, 387, 462, 576, 929, 1048
FspBI CTAG 1 cut(s) 615
GlaI GCGC 1 cut(s) 58
GluI GCNGC 8 cut(s) 7, 96, 99, 387, 462, 576, 929, 1048
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 414, 913
HapII CCGG 7 cut(s) 26, 368, 398, 485, 512, 894, 914
HhaI GCGC 1 cut(s) 59
Hin1II CATG 7 cut(s) 329, 598, 661, 712, 739, 885, 946
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 6 cut(s) 115, 694, 779, 869, 918, 1040
HpaII CCGG 7 cut(s) 26, 368, 398, 485, 512, 894, 914
HphI GGTGA 2 cut(s) 661, 841
Hpy166II GTNNAC 6 cut(s) 119, 259, 499, 729, 778, 873
Hpy188I TCNGA 5 cut(s) 39, 189, 693, 813, 935
Hpy188III TCNNGA 3 cut(s) 248, 368, 623
Hpy8I GTNNAC 6 cut(s) 119, 259, 499, 729, 778, 873
HpyAV CCTTC 4 cut(s) 746, 761, 808, 1049
HpyCH4III ACNGT 2 cut(s) 22, 47
HpyCH4IV ACGT 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 174, 575
HpyF3I CTNAG 4 cut(s) 477, 932, 975, 1062
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 7 cut(s) 329, 598, 661, 712, 739, 885, 946
HspAI GCGC 1 cut(s) 57
KflI GGGWCCC 1 cut(s) 678
Kpn2I TCCGGA 1 cut(s) 367
KpnI GGTACC 1 cut(s) 485
Ksp22I TGATCA 2 cut(s) 322, 903
Kzo9I GATC 4 cut(s) 322, 401, 831, 903
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 8 cut(s) 18, 82, 110, 398, 473, 587, 940, 1034
LweI GCATC 6 cut(s) 118, 291, 365, 556, 714, 836
MaeI CTAG 1 cut(s) 615
MaeII ACGT 1 cut(s) 150
MalI GATC 4 cut(s) 324, 403, 833, 905
MboI GATC 4 cut(s) 322, 401, 831, 903
MboII GAAGA 3 cut(s) 307, 444, 541
MfeI CAATTG 1 cut(s) 82
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 4 cut(s) 82, 302, 581, 864
MlyI GAGTC 4 cut(s) 124, 773, 878, 912
MmeI TCCRAC 2 cut(s) 193, 818
Mph1103I ATGCAT 1 cut(s) 284
MroI TCCGGA 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 1026
MspA1I CMGCKG 2 cut(s) 389, 1047
MspI CCGG 7 cut(s) 26, 368, 398, 485, 512, 894, 914
MspR9I CCNGG 2 cut(s) 399, 915
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 2 cut(s) 448, 602
MwoI GCNNNNNNNGC 3 cut(s) 63, 174, 575
NciI CCSGG 2 cut(s) 399, 915
NcoI CCATGG 1 cut(s) 881
NdeI CATATG 1 cut(s) 900
NdeII GATC 4 cut(s) 322, 401, 831, 903
NlaIII CATG 7 cut(s) 329, 598, 661, 712, 739, 885, 946
NlaIV GGNNCC 3 cut(s) 483, 679, 680
NsiI ATGCAT 1 cut(s) 284
PctI GAATGC 2 cut(s) 448, 602
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 2 cut(s) 694, 1040
PkrI GCNGC 8 cut(s) 8, 97, 100, 388, 463, 577, 930, 1049
PleI GAGTC 4 cut(s) 123, 773, 877, 912
PpsI GAGTC 4 cut(s) 123, 773, 877, 912
PpuMI RGGWCCY 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 678
PspN4I GGNNCC 3 cut(s) 483, 679, 680
PspPI GGNCC 4 cut(s) 215, 678, 911, 985
PspPPI RGGWCCY 1 cut(s) 678
PsrI GAACNNNNNNTAC 2 cut(s) 251, 283
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 401
PvuII CAGCTG 2 cut(s) 389, 1047
RsaI GTAC 3 cut(s) 269, 380, 483
RsaNI GTAC 3 cut(s) 268, 379, 482
SalI GTCGAC 1 cut(s) 871
SatI GCNGC 8 cut(s) 7, 96, 99, 387, 462, 576, 929, 1048
Sau3AI GATC 4 cut(s) 322, 401, 831, 903
Sau96I GGNCC 4 cut(s) 215, 678, 911, 985
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 4 cut(s) 124, 773, 878, 912
ScrFI CCNGG 2 cut(s) 399, 915
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 6 cut(s) 118, 291, 365, 556, 714, 836
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 3 cut(s) 215, 678, 985
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 4 cut(s) 82, 302, 581, 864
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 397, 913
StyI CCWWGG 2 cut(s) 747, 881
TaaI ACNGT 2 cut(s) 22, 47
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 834, 872
TasI AATT 4 cut(s) 82, 302, 581, 864
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 2 cut(s) 694, 1040
TscAI CASTG 4 cut(s) 27, 261, 325, 473
TseI GCWGC 8 cut(s) 6, 95, 98, 386, 461, 575, 928, 1047
TspDTI ATGAA 4 cut(s) 56, 428, 570, 1023
TspGWI ACGGA 1 cut(s) 998
TspRI CASTG 4 cut(s) 27, 261, 325, 473
VpaK11BI GGWCC 3 cut(s) 215, 678, 985
XapI RAATTY 1 cut(s) 302
XmiI GTMKAC 4 cut(s) 118, 498, 728, 872
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 1 cut(s) 615
ZrmI AGTACT 1 cut(s) 380
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.