FvH4_4g37360

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009829
Physical Location & Seq
Forward (+)
33851006 .. 33855051
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37360.t1

Sequence Viewer

Length: 1425 bp
ATGTTGGAGAAGCTGCTGAGGGCTGGCATGAATGTTGCTCGCTTTAACTTCTCCCATGGCACTCACGACTACCACCAGGAGACCCTCAACAACCTCCGCACTGCCATGCACAACACTCAGATCCTCTGCGCCCTCATGCTCGATACCAAGGGACCTGAGATTCGGACTGGATTCCTCAAGGATGGGAAACCCATTCAACTTAAGGAAGGCCAGGAAATCACCATTACCACTGACTACAACATCAAGGGAGATCCAGAGACCATTTCCATGAGCTACAAAAAGCTGGCCGTGGACTTGAAGCCTGGAAATACCATTTTATGTGCAGATGGCACCATTACCCTCGCTGTCTTGTCTTGTGATCCAGCGGCTGGTATTGTGAGGTGCCGTTGTGAAAACAGTGCAATGCTGGGTGAGAGGAAAAATGTCAATCTTCCTGGTGTTGTGGTTGATCTTCCCACACTGACAGAGAAGGACAGGGAAGATATTCTGGGATGGGGTGTTCCTAACAACATTGATATGATTGCTCTTTCATTTGTACGCAAAGGATCAGATCTAGTTAATGTCCGTAAAGTTCTTGGACCCCATGCCAAGAACATACAGTTGATGTCAAAGGTTGAAAACCAGGAGGGTGTTATCAACTTTGATGAGATACTGCGTGAGACTGACTCATTCATGGTTGCACGAGGTGATCTTGGTATGGAAATCCCAGTTGAGAAAATTTTTCTGGCACAAAAAATGATGATATATAAGTGTAACCTTGTGGGAAAGCCTGTTGTCACGGCCACTCAGATGCTTGAATCCATGATCAAGTCTCCACGACCAACGCGTGCTGAAGCCACAGATGTAGCTAATGCTGTCCTTGATGGCACTGATTGTGTCATGCTTAGTGGTGAGAGTGCAGCTGGGTCCTATCCAGAACTCGCTGTGAAGATCATGGCTCGGATATGTATCGAGGCAGAATCTTCCCTTGACTATGGGGCTATCTTCAAGGAGATGATAAAGTCTACACCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCTGCTGTCCGAACAGCCAACAAGGCAAGGGCCAAACTCATTGTTGTGTTGACACGTGGGGGGAGCACCGCAAAATTGGTGGCCAAGTACAGGCCAGCTGTTCCTATCCTGTCGGTGGTTGTTCCAGTTTTGACCACAGACTCGTTTGATTGGGACTGCAGTGATGAGACTCCAGCAAGACATAGCCTGATATACAGGGGTTTGATTCCTCTATTGGCTGAAGGATCTGCGAAGGCCACCGATTCTGAATCCACTGAGGTGATCCTGGATGCTGCTCTAAAGTCGGCAACAAAAAGGAGACTGTGCCAGCCTGGTGATGCTGTGGTGGCACTTCATCGTATCGGAGTTGCATCTGTCATCAAGATCTGCATAGTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

475

Amino Acids

51.46

Weight (kDa)

7.51

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 309 9.8e-142 Pyruvate kinase, barrel domain
PK_C PF02887 344 - 464 5.8e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009829)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08570 AT5G08570 AT5G08570 AT5G63680 AT5G63680 AT5G63680
fragaria_vesca FvH4_4g37360
malus_domestica MD13G1000500.v1.1 MD16G1005100.v1.1
prunus_persica Prupe.4G287000_v2.0.a1
pyrus_communis pycom2749g00020
rosa_chinensis RchiOBHm_Chr4g0447231
rosa_laevigata RLG00000005595
rosa_multiflora Rmu_ssc0000396.1_g000002
rosa_roxburghii Rroxscaffold_5G00387590
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4AG440400 Rh4BG449400 Rh4CG466400 Rh4DG447900
rosa_wichuraiana Rw4G037680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 329, 381
AccB7I CCANNNNNTGG 1 cut(s) 368
AccI GTMKAC 1 cut(s) 1004
AccII CGCG 1 cut(s) 826
AciI CCGC 3 cut(s) 97, 365, 1113
AclWI GGATC 6 cut(s) 115, 245, 353, 553, 1276, 1300
AcoI YGGCCR 3 cut(s) 285, 780, 1125
AcsI RAATTY 1 cut(s) 717
AcuI CTGAAG 2 cut(s) 852, 1284
AcvI CACGTG 1 cut(s) 1100
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 537, 1133
AfiI CCNNNNNNNGG 3 cut(s) 368, 1134, 1159
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 2 cut(s) 824, 1097
AgsI TTSAA 5 cut(s) 197, 298, 617, 797, 988
AjnI CCWGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
AleI CACNNNNGTG 1 cut(s) 1301
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
AluBI AGCT 6 cut(s) 13, 273, 283, 848, 902, 1142
AluI AGCT 6 cut(s) 13, 273, 283, 848, 902, 1142
Alw21I GWGCWC 1 cut(s) 1112
Alw26I GTCTC 6 cut(s) 74, 251, 653, 816, 1205, 1336
AlwI GGATC 6 cut(s) 115, 245, 353, 553, 1276, 1300
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 7 cut(s) 208, 285, 780, 1074, 1125, 1136, 1278
ApeKI GCWGC 3 cut(s) 13, 899, 1316
ApoI RAATTY 1 cut(s) 717
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 152, 578, 906, 1074
AsuHPI GGTGA 6 cut(s) 211, 422, 698, 902, 1315, 1370
AvaII GGWCC 3 cut(s) 152, 578, 906
BalI TGGCCA 1 cut(s) 1127
BanI GGYRCC 2 cut(s) 329, 381
BanII GRGCYC 1 cut(s) 1025
BauI CACGAG 1 cut(s) 681
BbrPI CACGTG 1 cut(s) 1100
Bbv12I GWGCWC 1 cut(s) 1112
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 2 cut(s) 911, 1303
BccI CCATC 4 cut(s) 176, 320, 486, 857
BceAI ACGGC 3 cut(s) 272, 369, 795
BciT130I CCWGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
BclI TGATCA 1 cut(s) 804
BcoDI GTCTC 6 cut(s) 74, 251, 653, 816, 1205, 1336
BfaI CTAG 1 cut(s) 554
BfmI CTRYAG 1 cut(s) 1201
BfrI CTTAAG 1 cut(s) 200
BglI GCCNNNNNGGC 1 cut(s) 1067
BglII AGATCT 2 cut(s) 550, 1407
BisI GCNGC 4 cut(s) 14, 366, 900, 1317
BlsI GCNGC 4 cut(s) 15, 367, 901, 1318
Bme1390I CCNGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
Bme18I GGWCC 3 cut(s) 152, 578, 906
BmgT120I GGNCC 4 cut(s) 152, 578, 906, 1074
BmiI GGNNCC 5 cut(s) 153, 331, 383, 580, 907
BmrFI CCNGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
BmrI ACTGGG 1 cut(s) 701
BmsI GCATC 5 cut(s) 780, 1049, 1303, 1351, 1403
BmuI ACTGGG 1 cut(s) 701
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 1 cut(s) 1200
Bpu10I CCTNAGC 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 1100
BsaBI GATNNNNATC 1 cut(s) 947
BsaI GGTCTC 2 cut(s) 74, 251
BsaJI CCNNGG 3 cut(s) 55, 147, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 1134, 1159
Bse1I ACTGG 3 cut(s) 172, 707, 1169
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 947
BseBI CCWGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
BseDI CCNNGG 3 cut(s) 55, 147, 288
BseGI GGATG 4 cut(s) 187, 497, 1040, 1318
BseJI GATNNNNATC 1 cut(s) 947
BseLI CCNNNNNNNGG 3 cut(s) 368, 1134, 1159
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 5 cut(s) 8, 131, 147, 800, 1290
BseNI ACTGG 3 cut(s) 172, 707, 1169
BseXI GCAGC 2 cut(s) 911, 1303
BseYI CCCAGC 2 cut(s) 406, 902
BsgI GTGCAG 2 cut(s) 342, 918
Bsh1236I CGCG 1 cut(s) 826
BshFI GGCC 7 cut(s) 210, 287, 782, 1076, 1127, 1138, 1280
BshNI GGYRCC 2 cut(s) 329, 381
BsiHKAI GWGCWC 1 cut(s) 1112
BslFI GGGAC 2 cut(s) 165, 1211
BslI CCNNNNNNNGG 3 cut(s) 368, 1134, 1159
BsmAI GTCTC 6 cut(s) 74, 251, 653, 816, 1205, 1336
BsmFI GGGAC 2 cut(s) 165, 1211
BsnI GGCC 7 cut(s) 210, 287, 782, 1076, 1127, 1138, 1280
Bso31I GGTCTC 2 cut(s) 74, 251
Bsp1286I GDGCHC 2 cut(s) 1025, 1112
Bsp19I CCATGG 1 cut(s) 55
BspACI CCGC 3 cut(s) 97, 365, 1113
BspANI GGCC 7 cut(s) 210, 287, 782, 1076, 1127, 1138, 1280
BspCNI CTCAG 5 cut(s) 9, 130, 148, 799, 1291
BspFNI CGCG 1 cut(s) 826
BspLI GGNNCC 5 cut(s) 153, 331, 383, 580, 907
BspMAI CTGCAG 1 cut(s) 1205
BspPI GGATC 6 cut(s) 115, 245, 353, 553, 1276, 1300
BspT107I GGYRCC 2 cut(s) 329, 381
BspTI CTTAAG 1 cut(s) 200
BspTNI GGTCTC 2 cut(s) 74, 251
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 172, 707, 1169
BssECI CCNNGG 3 cut(s) 55, 147, 288
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 55, 147
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
Bst4CI ACNGT 3 cut(s) 398, 600, 1347
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBAI YACGTR 1 cut(s) 1100
BstC8I GCNNGC 6 cut(s) 25, 40, 285, 828, 1140, 1352
BstDEI CTNAG 6 cut(s) 17, 117, 156, 786, 884, 1299
BstDSI CCRYGG 2 cut(s) 55, 288
BstF5I GGATG 4 cut(s) 187, 497, 1040, 1318
BstFNI CGCG 1 cut(s) 826
BstHHI GCGC 1 cut(s) 131
BstMAI GTCTC 6 cut(s) 74, 251, 653, 816, 1205, 1336
BstMWI GCNNNNNNNGC 3 cut(s) 1046, 1067, 1370
BstNI CCWGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
BstSCI CCNGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
BstSFI CTRYAG 1 cut(s) 1201
BstUI CGCG 1 cut(s) 826
BstV1I GCAGC 2 cut(s) 911, 1303
BstX2I RGATCY 5 cut(s) 120, 250, 550, 1268, 1407
BstYI RGATCY 5 cut(s) 120, 250, 550, 1268, 1407
BsuRI GGCC 7 cut(s) 210, 287, 782, 1076, 1127, 1138, 1280
BtgI CCRYGG 2 cut(s) 55, 288
BtsCI GGATG 4 cut(s) 187, 497, 1040, 1318
BtsI GCAGTG 2 cut(s) 99, 1210
BtsIMutI CAGTG 7 cut(s) 99, 228, 403, 458, 867, 1210, 1296
Cac8I GCNNGC 6 cut(s) 25, 40, 285, 828, 1140, 1352
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 4 cut(s) 152, 578, 906, 1074
Csp6I GTAC 2 cut(s) 536, 1132
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CviQI GTAC 2 cut(s) 536, 1132
DdeI CTNAG 6 cut(s) 17, 117, 156, 786, 884, 1299
DraIII CACNNNGTG 1 cut(s) 686
EaeI YGGCCR 3 cut(s) 285, 780, 1125
Eco130I CCWWGG 2 cut(s) 55, 147
Eco24I GRGCYC 1 cut(s) 1025
Eco31I GGTCTC 2 cut(s) 74, 251
Eco47I GGWCC 3 cut(s) 152, 578, 906
Eco57I CTGAAG 2 cut(s) 852, 1284
Eco72I CACGTG 1 cut(s) 1100
EcoO109I RGGNCCY 2 cut(s) 152, 906
EcoRII CCWGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
EcoT14I CCWWGG 2 cut(s) 55, 147
EcoT38I GRGCYC 1 cut(s) 1025
ErhI CCWWGG 2 cut(s) 55, 147
FaqI GGGAC 2 cut(s) 165, 1211
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 1004
Fnu4HI GCNGC 4 cut(s) 14, 366, 900, 1317
FokI GGATG 4 cut(s) 194, 504, 1027, 1325
FriOI GRGCYC 1 cut(s) 1025
Fsp4HI GCNGC 4 cut(s) 14, 366, 900, 1317
FspBI CTAG 1 cut(s) 554
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 14, 366, 900, 1317
GsaI CCCAGC 2 cut(s) 410, 906
GsuI CTGGAG 1 cut(s) 1200
HaeIII GGCC 7 cut(s) 210, 287, 782, 1076, 1127, 1138, 1280
HhaI GCGC 1 cut(s) 131
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HphI GGTGA 6 cut(s) 211, 422, 698, 902, 1315, 1370
Hpy166II GTNNAC 3 cut(s) 292, 1005, 1095
Hpy188I TCNGA 8 cut(s) 120, 165, 550, 789, 942, 1055, 1291, 1388
Hpy188III TCNNGA 4 cut(s) 65, 254, 914, 1405
Hpy8I GTNNAC 3 cut(s) 292, 1005, 1095
HpyAV CCTTC 4 cut(s) 200, 463, 1259, 1270
HpyCH4III ACNGT 3 cut(s) 398, 600, 1347
HpyCH4IV ACGT 1 cut(s) 1099
HpyCH4V TGCA 9 cut(s) 109, 323, 401, 680, 899, 1040, 1203, 1394, 1413
HpyF10VI GCNNNNNNNGC 3 cut(s) 1046, 1067, 1370
HpyF3I CTNAG 6 cut(s) 17, 117, 156, 786, 884, 1299
HpySE526I ACGT 1 cut(s) 1099
HspAI GCGC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 804
LmnI GCTCC 1 cut(s) 1107
Lsp1109I GCAGC 2 cut(s) 911, 1303
LweI GCATC 5 cut(s) 780, 1049, 1303, 1351, 1403
MaeI CTAG 1 cut(s) 554
MaeII ACGT 1 cut(s) 1099
MaeIII GTNAC 2 cut(s) 752, 775
MboII GAAGA 6 cut(s) 422, 443, 491, 940, 954, 976
MflI RGATCY 5 cut(s) 120, 250, 550, 1268, 1407
MhlI GDGCHC 2 cut(s) 1025, 1112
MlsI TGGCCA 1 cut(s) 1127
MluCI AATT 2 cut(s) 717, 1118
MluI ACGCGT 1 cut(s) 824
MluNI TGGCCA 1 cut(s) 1127
MlyI GAGTC 4 cut(s) 659, 1042, 1178, 1207
Mox20I TGGCCA 1 cut(s) 1127
MroXI GAANNNNTTC 1 cut(s) 483
MscI TGGCCA 1 cut(s) 1127
MseI TTAA 3 cut(s) 45, 201, 558
MslI CAYNNNNRTG 6 cut(s) 104, 266, 515, 788, 1088, 1301
Msp20I TGGCCA 1 cut(s) 1127
MspA1I CMGCKG 3 cut(s) 365, 902, 1142
MspCI CTTAAG 1 cut(s) 200
MspR9I CCNGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
MvaI CCWGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
MvnI CGCG 1 cut(s) 826
MwoI GCNNNNNNNGC 3 cut(s) 1046, 1067, 1370
NcoI CCATGG 1 cut(s) 55
NlaIV GGNNCC 5 cut(s) 153, 331, 383, 580, 907
NmuCI GTSAC 1 cut(s) 775
OliI CACNNNNGTG 1 cut(s) 1301
PcsI WCGNNNNNNNCGW 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 7 cut(s) 160, 171, 797, 959, 1249, 1286, 1292
PflMI CCANNNNNTGG 1 cut(s) 368
PfoI TCCNGGA 1 cut(s) 1308
PkrI GCNGC 4 cut(s) 15, 367, 901, 1318
PleI GAGTC 4 cut(s) 659, 1041, 1178, 1207
PmaCI CACGTG 1 cut(s) 1100
PmlI CACGTG 1 cut(s) 1100
PpsI GAGTC 4 cut(s) 659, 1041, 1178, 1207
Ppu21I YACGTR 1 cut(s) 1100
PpuMI RGGWCCY 2 cut(s) 152, 906
Psp5II RGGWCCY 2 cut(s) 152, 906
Psp6I CCWGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
PspCI CACGTG 1 cut(s) 1100
PspFI CCCAGC 2 cut(s) 406, 902
PspGI CCWGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
PspN4I GGNNCC 5 cut(s) 153, 331, 383, 580, 907
PspPI GGNCC 4 cut(s) 152, 578, 906, 1074
PspPPI RGGWCCY 2 cut(s) 152, 906
PstI CTGCAG 1 cut(s) 1205
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 5 cut(s) 120, 250, 550, 1268, 1407
PvuII CAGCTG 2 cut(s) 902, 1142
RsaI GTAC 2 cut(s) 537, 1133
RsaNI GTAC 2 cut(s) 536, 1132
RseI CAYNNNNRTG 6 cut(s) 104, 266, 515, 788, 1088, 1301
SaqAI TTAA 3 cut(s) 45, 201, 558
SatI GCNGC 4 cut(s) 14, 366, 900, 1317
Sau96I GGNCC 4 cut(s) 152, 578, 906, 1074
SchI GAGTC 4 cut(s) 659, 1042, 1178, 1207
ScrFI CCNGG 7 cut(s) 77, 212, 303, 435, 623, 1310, 1356
SduI GDGCHC 2 cut(s) 1025, 1112
SfaNI GCATC 5 cut(s) 780, 1049, 1303, 1351, 1403
SfcI CTRYAG 1 cut(s) 1201
SinI GGWCC 3 cut(s) 152, 578, 906
SmiMI CAYNNNNRTG 6 cut(s) 104, 266, 515, 788, 1088, 1301
SmlI CTYRAG 2 cut(s) 176, 200
SmoI CTYRAG 2 cut(s) 176, 200
Sse9I AATT 2 cut(s) 717, 1118
SsiI CCGC 3 cut(s) 97, 365, 1113
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 7 cut(s) 75, 210, 301, 433, 621, 1308, 1354
StyI CCWWGG 2 cut(s) 55, 147
TaaI ACNGT 3 cut(s) 398, 600, 1347
TaiI ACGT 1 cut(s) 1102
TaqI TCGA 2 cut(s) 141, 951
TasI AATT 2 cut(s) 717, 1118
TatI WGTACW 1 cut(s) 1131
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 7 cut(s) 160, 171, 797, 959, 1249, 1286, 1292
Tru1I TTAA 3 cut(s) 45, 201, 558
Tru9I TTAA 3 cut(s) 45, 201, 558
TscAI CASTG 7 cut(s) 106, 235, 403, 465, 874, 1210, 1303
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 3 cut(s) 13, 899, 1316
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 4 cut(s) 44, 519, 661, 1367
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 7 cut(s) 106, 235, 403, 465, 874, 1210, 1303
Van91I CCANNNNNTGG 1 cut(s) 368
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 3 cut(s) 152, 578, 906
XapI RAATTY 1 cut(s) 717
XmiI GTMKAC 1 cut(s) 1004
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.