MD16G1005100.v1.1

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009829
Physical Location & Seq
Forward (+)
381855 .. 385302
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1005100.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGCCAACATAGACATAGAAGGAATACTCAAGGAGCTCCCCAACGACGGCCGCATCCCCAAGACCAAGATCGTCTGCACCCTCGGCCCCTCTTCCCGCTCGGTCCCCATGGTCGAGAAGCTTCTCAGGGCTGGCATGAACGTCGCCCGCTTCAACTTCTCTCACGGCACCCACGACTACCACCAGGAGACCCTCGACAATCTCCGCACTGCCATGCACAACACTCAGATCCTCTGCGCCGTGATGCTTGATACAAAGGGGCCTGAGATTCGGACTGGATTCCTCAAGGGTGGAAAACCGATTCAACTTAAGGAAGGGCAGGAAATTACTATTACTACTGATTACAGCATCAAGGGGGATGCAGAGATGATTTCCATGAGCTACAAGAAGCTTGCTGTGGATTTGAAGCCTAGAAATACCATTTTATGTGCAGATGGCACCATTACTCTCACTGTATTATCTTGTGATCCGGCAGCTGGTACAGTTAGGTGCCGCTGTGAGAACACTGCAATGCTGGGTGAAAGAAAAAATGTCAATCTTCCTGGTGTTGTGGTGGATCTTCCCACACTGACGGAGAAGGATAAGGAAGATATTCTCGAATGGGGCGTGCCCAACAAGATTGATATGATTGCTCTTTCATTTGTACGCAAGGGATCAGATCTTGTTAATGTCCGTAAAGTTCTTGGACCCCATGCCAAGAACATACAATTGATGTCAAAGGTTGAAAACCAGGAGGGAGTTATCAACTTTGACGAGATATTGCGAGAGACAGATTCATTCATGGTTGCCCGTGGTGATCTTGGAATGGAAATCCCAGTTGAGAAAATTTTTCTGGCACAAAAGATGATGATATATAAGTGTAATCTTGTGGGCAAGCCTGTGGTCACTGCCACTCAGATGCTTGAATCCATGATCAAGTCTCCTCGGCCAACCCGTGCTGAAGCCACAGATGTAGCTAATGCTGTCCTTGATGGCACTGATTGTGTCATGCTTAGTGGTGAGAGTGCAGCTGGGTCCTATCCAGAACTTGCTGTGAAAATTATGGCTCGTATATGTATTGAGGCAGAATCTTCCCTTGATTACAGGGCAATCTTTAAGGAGATGATAAAGTCGACACCCCTTCCAATGAGCCCTTTGGAGAGTCTTGCATCCTCTGCTGTCAGAACAGCCAACAAGGCCAGAGCCAAACTCATTGTTGTGTTGACACGTGGTGGGAGCACAGCCAAATTGGTGGCGAAGTACCGGCCTGCTGTTCCTATCCTGTCGGTGGTGGTTCCAGTTCTGACCACAGACTCATTTGATTGGACCTGCAGTGATGAGACTCCGGCAAGGCATAGCCTAATCTACAGGGGGTTGATTCCTCTATTGGCTGAAGGATCTGCCAAAGCTACTGATGCAGAATCCACAGAGGTGATCCTGGAAGCTGCTCTGAAGTCAGCAACAGAGAGGGGATTGTGCACGCCTGGGGATGCTGTGGTGGCACTTCATCGTATTGGAGTCGCCTCTGTTATTAAGATCTGCGTGGTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

511

Amino Acids

55.33

Weight (kDa)

7.89

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 20 - 345 2.6e-151 Pyruvate kinase, barrel domain
PK_C PF02887 380 - 500 1.1e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009829)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08570 AT5G08570 AT5G08570 AT5G63680 AT5G63680 AT5G63680
fragaria_vesca FvH4_4g37360
malus_domestica MD13G1000500.v1.1 MD16G1005100.v1.1
prunus_persica Prupe.4G287000_v2.0.a1
pyrus_communis pycom2749g00020
rosa_chinensis RchiOBHm_Chr4g0447231
rosa_laevigata RLG00000005595
rosa_multiflora Rmu_ssc0000396.1_g000002
rosa_roxburghii Rroxscaffold_5G00387590
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4AG440400 Rh4BG449400 Rh4CG466400 Rh4DG447900
rosa_wichuraiana Rw4G037680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1316
AccB1I GGYRCC 3 cut(s) 167, 437, 489
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 1112
AciI CCGC 5 cut(s) 52, 97, 148, 205, 493
AclWI GGATC 6 cut(s) 223, 461, 564, 661, 1384, 1408
AcoI YGGCCR 3 cut(s) 3, 49, 926
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 3 cut(s) 960, 1392, 1451
AcvI CACGTG 1 cut(s) 1208
AdeI CACNNNGTG 1 cut(s) 1211
AfaI GTAC 3 cut(s) 481, 645, 1241
AfiI CCNNNNNNNGG 3 cut(s) 47, 476, 1267
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 1205
AgsI TTSAA 5 cut(s) 154, 305, 406, 725, 905
AjnI CCWGG 5 cut(s) 183, 541, 729, 1416, 1462
AleI CACNNNNGTG 1 cut(s) 1409
AluBI AGCT 9 cut(s) 37, 121, 381, 391, 476, 956, 1010, 1388, 1424
AluI AGCT 9 cut(s) 37, 121, 381, 391, 476, 956, 1010, 1388, 1424
Alw21I GWGCWC 3 cut(s) 39, 1220, 1460
Alw26I GTCTC 4 cut(s) 182, 761, 924, 1313
Alw44I GTGCAC 1 cut(s) 1456
AlwI GGATC 6 cut(s) 223, 461, 564, 661, 1384, 1408
AoxI GGCC 7 cut(s) 3, 49, 85, 260, 926, 1176, 1244
ApaLI GTGCAC 1 cut(s) 1456
ApeKI GCWGC 3 cut(s) 473, 1007, 1424
ApoI RAATTY 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 6 cut(s) 86, 103, 260, 686, 1014, 1305
AsuHPI GGTGA 4 cut(s) 530, 806, 1010, 1423
AvaII GGWCC 4 cut(s) 103, 686, 1014, 1305
BaeGI GKGCMC 2 cut(s) 612, 1460
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 167, 437, 489
BanII GRGCYC 2 cut(s) 39, 1133
BbrPI CACGTG 1 cut(s) 1208
Bbv12I GWGCWC 3 cut(s) 39, 1220, 1460
BbvI GCAGC 3 cut(s) 485, 1019, 1411
BccI CCATC 2 cut(s) 428, 965
BceAI ACGGC 3 cut(s) 64, 181, 224
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 5 cut(s) 185, 543, 731, 1418, 1464
BclI TGATCA 1 cut(s) 912
BcoDI GTCTC 4 cut(s) 182, 761, 924, 1313
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 2 cut(s) 1309, 1345
BfrI CTTAAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 1316
BglI GCCNNNNNGGC 1 cut(s) 1175
BglII AGATCT 2 cut(s) 658, 1515
BisI GCNGC 5 cut(s) 52, 474, 493, 1008, 1425
BlsI GCNGC 5 cut(s) 53, 475, 494, 1009, 1426
Bme1390I CCNGG 5 cut(s) 185, 543, 731, 1418, 1464
Bme18I GGWCC 4 cut(s) 103, 686, 1014, 1305
BmgT120I GGNCC 6 cut(s) 86, 103, 260, 686, 1014, 1305
BmiI GGNNCC 9 cut(s) 88, 105, 169, 261, 439, 491, 688, 1015, 1275
BmrFI CCNGG 5 cut(s) 185, 543, 731, 1418, 1464
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 8 cut(s) 63, 234, 349, 357, 888, 1157, 1384, 1459
BmuI ACTGGG 1 cut(s) 809
BplI GAGNNNNNCTC 2 cut(s) 1173, 1205
BpuEI CTTGAG 2 cut(s) 14, 269
BsaAI YACGTR 1 cut(s) 1208
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 5 cut(s) 82, 108, 790, 923, 1463
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 476, 1267
Bse118I RCCGGY 1 cut(s) 1242
Bse1I ACTGG 3 cut(s) 280, 815, 1277
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 5 cut(s) 185, 543, 731, 1418, 1464
BseDI CCNNGG 5 cut(s) 82, 108, 790, 923, 1463
BseGI GGATG 4 cut(s) 54, 364, 1148, 1474
BseLI CCNNNNNNNGG 3 cut(s) 47, 476, 1267
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 4 cut(s) 139, 239, 255, 908
BseNI ACTGG 3 cut(s) 280, 815, 1277
BseRI GAGGAG 1 cut(s) 912
BseSI GKGCMC 2 cut(s) 612, 1460
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 485, 1019, 1411
BseYI CCCAGC 2 cut(s) 514, 1010
BsgI GTGCAG 3 cut(s) 61, 450, 1026
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 7 cut(s) 5, 51, 87, 262, 928, 1178, 1246
BshNI GGYRCC 3 cut(s) 167, 437, 489
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 3 cut(s) 39, 1220, 1460
BsiSI CCGG 3 cut(s) 470, 1243, 1325
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 3 cut(s) 47, 476, 1267
BsmAI GTCTC 4 cut(s) 182, 761, 924, 1313
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 7 cut(s) 5, 51, 87, 262, 928, 1178, 1246
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 5 cut(s) 39, 612, 1133, 1220, 1460
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 5 cut(s) 52, 97, 148, 205, 493
BspANI GGCC 7 cut(s) 5, 51, 87, 262, 928, 1178, 1246
BspCNI CTCAG 4 cut(s) 138, 238, 256, 907
BspLI GGNNCC 9 cut(s) 88, 105, 169, 261, 439, 491, 688, 1015, 1275
BspMAI CTGCAG 1 cut(s) 1313
BspMI ACCTGC 1 cut(s) 1316
BspPI GGATC 6 cut(s) 223, 461, 564, 661, 1384, 1408
BspT107I GGYRCC 3 cut(s) 167, 437, 489
BspTI CTTAAG 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 1242
BsrI ACTGG 3 cut(s) 280, 815, 1277
BssAI RCCGGY 1 cut(s) 1242
BssECI CCNNGG 5 cut(s) 82, 108, 790, 923, 1463
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 5 cut(s) 185, 543, 731, 1418, 1464
Bst4CI ACNGT 2 cut(s) 454, 484
Bst6I CTCTTC 1 cut(s) 97
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstBAI YACGTR 1 cut(s) 1208
BstC8I GCNNGC 7 cut(s) 133, 148, 393, 608, 875, 1248, 1460
BstDEI CTNAG 5 cut(s) 125, 225, 264, 894, 992
BstDSI CCRYGG 2 cut(s) 108, 790
BstF5I GGATG 4 cut(s) 54, 364, 1148, 1474
BstHHI GCGC 1 cut(s) 239
BstMAI GTCTC 4 cut(s) 182, 761, 924, 1313
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 5 cut(s) 84, 1154, 1175, 1394, 1478
BstNI CCWGG 5 cut(s) 185, 543, 731, 1418, 1464
BstSCI CCNGG 5 cut(s) 183, 541, 729, 1416, 1462
BstSFI CTRYAG 2 cut(s) 1309, 1345
BstSLI GKGCMC 2 cut(s) 612, 1460
BstV1I GCAGC 3 cut(s) 485, 1019, 1411
BstX2I RGATCY 5 cut(s) 228, 556, 658, 1376, 1515
BstXI CCANNNNNNTGG 1 cut(s) 1231
BstYI RGATCY 5 cut(s) 228, 556, 658, 1376, 1515
BstZI CGGCCG 1 cut(s) 49
BsuRI GGCC 7 cut(s) 5, 51, 87, 262, 928, 1178, 1246
BtgI CCRYGG 2 cut(s) 108, 790
BtsCI GGATG 4 cut(s) 54, 364, 1148, 1474
BtsI GCAGTG 4 cut(s) 207, 504, 885, 1318
BtsIMutI CAGTG 7 cut(s) 207, 450, 504, 566, 885, 975, 1318
BveI ACCTGC 1 cut(s) 1316
Cac8I GCNNGC 7 cut(s) 133, 148, 393, 608, 875, 1248, 1460
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 1242
Cfr13I GGNCC 6 cut(s) 86, 103, 260, 686, 1014, 1305
Csp6I GTAC 3 cut(s) 480, 644, 1240
CviAII CATG 8 cut(s) 109, 136, 214, 376, 692, 781, 910, 988
CviQI GTAC 3 cut(s) 480, 644, 1240
DdeI CTNAG 5 cut(s) 125, 225, 264, 894, 992
DraIII CACNNNGTG 1 cut(s) 1211
EaeI YGGCCR 3 cut(s) 3, 49, 926
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 37
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 2 cut(s) 39, 1133
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 4 cut(s) 103, 686, 1014, 1305
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 3 cut(s) 960, 1392, 1451
Eco72I CACGTG 1 cut(s) 1208
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 2 cut(s) 260, 1014
EcoRII CCWGG 5 cut(s) 183, 541, 729, 1416, 1462
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 2 cut(s) 39, 1133
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 8 cut(s) 112, 139, 217, 379, 695, 784, 913, 991
FaqI GGGAC 1 cut(s) 89
FatI CATG 8 cut(s) 108, 135, 213, 375, 691, 780, 909, 987
FauI CCCGC 2 cut(s) 104, 155
FbaI TGATCA 1 cut(s) 912
FblI GTMKAC 1 cut(s) 1112
Fnu4HI GCNGC 5 cut(s) 52, 474, 493, 1008, 1425
FokI GGATG 4 cut(s) 41, 371, 1135, 1481
FriOI GRGCYC 2 cut(s) 39, 1133
Fsp4HI GCNGC 5 cut(s) 52, 474, 493, 1008, 1425
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 238
GluI GCNGC 5 cut(s) 52, 474, 493, 1008, 1425
GsaI CCCAGC 2 cut(s) 518, 1014
HaeIII GGCC 7 cut(s) 5, 51, 87, 262, 928, 1178, 1246
HapII CCGG 3 cut(s) 470, 1243, 1325
HhaI GCGC 1 cut(s) 239
Hin1II CATG 8 cut(s) 112, 139, 217, 379, 695, 784, 913, 991
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 2 cut(s) 1113, 1203
HindII GTYRAC 2 cut(s) 1113, 1203
HindIII AAGCTT 2 cut(s) 119, 389
HpaII CCGG 3 cut(s) 470, 1243, 1325
HphI GGTGA 4 cut(s) 530, 806, 1010, 1423
Hpy166II GTNNAC 3 cut(s) 1113, 1203, 1458
Hpy188I TCNGA 7 cut(s) 228, 273, 658, 897, 1163, 1284, 1431
Hpy188III TCNNGA 3 cut(s) 115, 596, 1022
Hpy8I GTNNAC 3 cut(s) 1113, 1203, 1458
Hpy99I CGWCG 2 cut(s) 50, 146
HpyAV CCTTC 5 cut(s) 14, 308, 571, 1130, 1367
HpyCH4III ACNGT 2 cut(s) 454, 484
HpyCH4IV ACGT 2 cut(s) 141, 1207
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 1154, 1175, 1394, 1478
HpyF3I CTNAG 5 cut(s) 125, 225, 264, 894, 992
HpySE526I ACGT 2 cut(s) 141, 1207
Hsp92II CATG 8 cut(s) 112, 139, 217, 379, 695, 784, 913, 991
HspAI GCGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 912
LmnI GCTCC 3 cut(s) 34, 42, 1215
Lsp1109I GCAGC 3 cut(s) 485, 1019, 1411
LweI GCATC 8 cut(s) 63, 234, 349, 357, 888, 1157, 1384, 1459
MaeI CTAG 1 cut(s) 411
MaeII ACGT 2 cut(s) 141, 1207
MaeIII GTNAC 1 cut(s) 883
MbiI CCGCTC 1 cut(s) 99
MboII GAAGA 5 cut(s) 84, 530, 551, 599, 1062
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 5 cut(s) 228, 556, 658, 1376, 1515
MhlI GDGCHC 5 cut(s) 39, 612, 1133, 1220, 1460
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 324, 707, 825, 1038, 1226
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 1150, 1286, 1315, 1506
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 591
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 309, 666, 1095, 1512
MslI CAYNNNNRTG 5 cut(s) 212, 509, 896, 1196, 1409
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 476, 495, 1010
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 3 cut(s) 470, 1243, 1325
MspR9I CCNGG 5 cut(s) 185, 543, 731, 1418, 1464
MunI CAATTG 1 cut(s) 707
MvaI CCWGG 5 cut(s) 185, 543, 731, 1418, 1464
MwoI GCNNNNNNNGC 5 cut(s) 84, 1154, 1175, 1394, 1478
NcoI CCATGG 1 cut(s) 108
NlaIII CATG 8 cut(s) 112, 139, 217, 379, 695, 784, 913, 991
NlaIV GGNNCC 9 cut(s) 88, 105, 169, 261, 439, 491, 688, 1015, 1275
NmeAIII GCCGAG 2 cut(s) 63, 904
NmuCI GTSAC 1 cut(s) 883
OliI CACNNNNGTG 1 cut(s) 1409
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 603, 931
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 8 cut(s) 268, 279, 301, 773, 905, 1067, 1357, 1400
PfoI TCCNGGA 1 cut(s) 1416
PkrI GCNGC 5 cut(s) 53, 475, 494, 1009, 1426
PleI GAGTC 4 cut(s) 1149, 1286, 1315, 1505
PmaCI CACGTG 1 cut(s) 1208
PmlI CACGTG 1 cut(s) 1208
PpsI GAGTC 4 cut(s) 1149, 1286, 1315, 1505
Ppu21I YACGTR 1 cut(s) 1208
PpuMI RGGWCCY 1 cut(s) 1014
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 5 cut(s) 183, 541, 729, 1416, 1462
PspCI CACGTG 1 cut(s) 1208
PspFI CCCAGC 2 cut(s) 514, 1010
PspGI CCWGG 5 cut(s) 183, 541, 729, 1416, 1462
PspN4I GGNNCC 9 cut(s) 88, 105, 169, 261, 439, 491, 688, 1015, 1275
PspPI GGNCC 6 cut(s) 86, 103, 260, 686, 1014, 1305
PspPPI RGGWCCY 1 cut(s) 1014
PstI CTGCAG 1 cut(s) 1313
PsuI RGATCY 5 cut(s) 228, 556, 658, 1376, 1515
PvuII CAGCTG 2 cut(s) 476, 1010
RsaI GTAC 3 cut(s) 481, 645, 1241
RsaNI GTAC 3 cut(s) 480, 644, 1240
RseI CAYNNNNRTG 5 cut(s) 212, 509, 896, 1196, 1409
SacI GAGCTC 1 cut(s) 39
SalI GTCGAC 1 cut(s) 1111
SaqAI TTAA 4 cut(s) 309, 666, 1095, 1512
SatI GCNGC 5 cut(s) 52, 474, 493, 1008, 1425
Sau96I GGNCC 6 cut(s) 86, 103, 260, 686, 1014, 1305
SchI GAGTC 4 cut(s) 1150, 1286, 1315, 1506
ScrFI CCNGG 5 cut(s) 185, 543, 731, 1418, 1464
SduI GDGCHC 5 cut(s) 39, 612, 1133, 1220, 1460
SfaNI GCATC 8 cut(s) 63, 234, 349, 357, 888, 1157, 1384, 1459
SfcI CTRYAG 2 cut(s) 1309, 1345
SinI GGWCC 4 cut(s) 103, 686, 1014, 1305
SmiMI CAYNNNNRTG 5 cut(s) 212, 509, 896, 1196, 1409
SmlI CTYRAG 3 cut(s) 29, 284, 308
SmoI CTYRAG 3 cut(s) 29, 284, 308
Sse9I AATT 5 cut(s) 324, 707, 825, 1038, 1226
SsiI CCGC 5 cut(s) 52, 97, 148, 205, 493
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 183, 541, 729, 1416, 1462
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 454, 484
TaiI ACGT 2 cut(s) 144, 1210
TaqI TCGA 4 cut(s) 114, 195, 597, 1112
TaqII GACCGA 1 cut(s) 91
TasI AATT 5 cut(s) 324, 707, 825, 1038, 1226
TauI GCSGC 2 cut(s) 54, 495
TfiI GAWTC 8 cut(s) 268, 279, 301, 773, 905, 1067, 1357, 1400
Tru1I TTAA 4 cut(s) 309, 666, 1095, 1512
Tru9I TTAA 4 cut(s) 309, 666, 1095, 1512
TscAI CASTG 7 cut(s) 214, 457, 511, 573, 892, 982, 1318
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 3 cut(s) 473, 1007, 1424
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 5 cut(s) 152, 627, 765, 769, 1475
TspGWI ACGGA 2 cut(s) 587, 662
TspRI CASTG 7 cut(s) 214, 457, 511, 573, 892, 982, 1318
Vha464I CTTAAG 1 cut(s) 308
VneI GTGCAC 1 cut(s) 1456
VpaK11BI GGWCC 4 cut(s) 103, 686, 1014, 1305
XapI RAATTY 1 cut(s) 825
XmiI GTMKAC 1 cut(s) 1112
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.