Rh4AG440400

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009829
Physical Location & Seq
Forward (+)
73141440 .. 73144601
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG440400.1

Sequence Viewer

Length: 1533 bp
ATGGCGAACATAGACATAGAGGGAATACTGAAGGAGCTGCCCAACGATGGGCGTATCCCGAAGACGAAGATCGTGTGTACTTTGGGTCCTTCATCTCGGTCGGTGGAGATGTTGGAGAAGCTGCTGAGGGCTGGCATGAATGTTGCTCGCTTTAACTTCTCCCATGGCACTCATGACTACCACCAGGAGACCCTCAACAATCTCCGCACTGCCATGCACAACACTCAGATCCTCTGCGCCGTCATGCTCGATACCAAGGGACCTGAGATTCGGACTGGATTCCTCAAGGATGGGAAGCCTATTCAACTTAAAGAAGGCCAGGAAATCACCATTACTACTGACTATACCATCAAGGGTGATCCAGAGACCATCTCCATGAGCTACAAGAAGCTGGCCGTGGACTTGAAGCCTGGAAACACCATTTTATGTGCGGACGGCACCATTACCCTTAATGTGTTGTCTTGTGATCCAGCGGCTGGTATTGTGAGGTGCCGTTGTGGGAACACTGCATTGCTGGGTGAGAGGAAAAATGTCAATCTTCCTGGTGTTGTGGTGGATCTTCCCACACTGACAGAGAAGGACAAGGAAGATATTCTGGGATGGGGTGTTCCCAACAACATTGATATGATTGCTCTTTCATTTGTACGCAAGGGATCAGATCTTGTTAATGTCCGTAAAGTTCTTGGACCCCATGCCAAGAACATACAGTTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAACTTTGATGAGATACTGCGTGAGACCGACTCATTCATGGTTGCACGAGGCGATCTTGGTATGGAAATCCCAGTTGAGAAAATTTTTCTGGCACAAAAAATGATGATATATAAGTGTAATCTTGTGGGCAAGCCTGTTGTCACTGCCACTCAGATGCTTGAATCCATGATCAAGTCTCCACGGCCAACGCGTGCTGAAGCCACAGATGTAGCTAATGCTGTCCTTGATGGCACTGATTGTGTCATGCTTAGTGGTGAGAGTGCAGCTGGGTCCTATCCAGAACTCGCTGTGAAGATCATGGCTCGAATATGTATTGAGGCAGAATCGTCCCTTGACTATGGGGCTATCTTTAAGGAGATGATAAAGTCTACACCGCTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCTGCTGTCCGAACAGCCAACAAGGCCAGAGCCAAACTCATTGTTGTGTTGACTCGTGGTGGGAGCACCGCAAAATTGGTAGCCAAGTACAGGCCAGCTGTTCCTATCCTATCTGTAGTTGTTCCAGTTTTGACCACGGACTCGTTTGATTGGGACTGCAGTGATGAGACTCCAGCAAGACATAGCCTGATCTACAGGGGTTTGATTCCTCTATTGGCTGAAGGATCTGCAAAGGCCACCGATTCCGAATCCACTGAGGTGATCCTGGATGCTGCTCTAAAGTCGGCAACAAAGAGGAGACTGTGCAAGCCTGGTGATGCTGTGGTGGCACTTCATCGTATTGGAGTTGCCTCGGTCATTAAGATTTGCGTAGTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

510

Amino Acids

55.24

Weight (kDa)

8.15

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 20 - 345 5.4e-152 Pyruvate kinase, barrel domain
PK_C PF02887 380 - 500 4.2e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009829)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08570 AT5G08570 AT5G08570 AT5G63680 AT5G63680 AT5G63680
fragaria_vesca FvH4_4g37360
malus_domestica MD13G1000500.v1.1 MD16G1005100.v1.1
prunus_persica Prupe.4G287000_v2.0.a1
pyrus_communis pycom2749g00020
rosa_chinensis RchiOBHm_Chr4g0447231
rosa_laevigata RLG00000005595
rosa_multiflora Rmu_ssc0000396.1_g000002
rosa_roxburghii Rroxscaffold_5G00387590
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4AG440400 Rh4BG449400 Rh4CG466400 Rh4DG447900
rosa_wichuraiana Rw4G037680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 437, 489
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 1 cut(s) 1112
AccII CGCG 1 cut(s) 934
AciI CCGC 5 cut(s) 205, 431, 473, 1118, 1221
AclWI GGATC 7 cut(s) 223, 353, 461, 564, 661, 1384, 1408
AcoI YGGCCR 2 cut(s) 393, 926
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 3 cut(s) 50, 960, 1392
AfaI GTAC 3 cut(s) 79, 645, 1241
AfiI CCNNNNNNNGG 4 cut(s) 47, 48, 476, 1242
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 3 cut(s) 305, 406, 905
AjnI CCWGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
AleI CACNNNNGTG 1 cut(s) 1409
AloI GAACNNNNNNTCC 2 cut(s) 591, 623
AluBI AGCT 7 cut(s) 37, 121, 381, 391, 956, 1010, 1250
AluI AGCT 7 cut(s) 37, 121, 381, 391, 956, 1010, 1250
Alw21I GWGCWC 1 cut(s) 1220
Alw26I GTCTC 6 cut(s) 182, 359, 761, 924, 1313, 1444
AlwI GGATC 7 cut(s) 223, 353, 461, 564, 661, 1384, 1408
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 6 cut(s) 316, 393, 926, 1176, 1244, 1386
ApeKI GCWGC 4 cut(s) 37, 121, 1007, 1424
ApoI RAATTY 1 cut(s) 825
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 4 cut(s) 86, 260, 686, 1014
AsuHPI GGTGA 6 cut(s) 319, 368, 530, 1010, 1423, 1478
AvaII GGWCC 4 cut(s) 86, 260, 686, 1014
BanI GGYRCC 2 cut(s) 437, 489
BanII GRGCYC 1 cut(s) 1133
BauI CACGAG 2 cut(s) 789, 1206
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 1220
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 4 cut(s) 24, 108, 1019, 1411
BccI CCATC 6 cut(s) 41, 284, 356, 377, 594, 965
BceAI ACGGC 5 cut(s) 224, 380, 451, 477, 941
BciT130I CCWGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
BciVI GTATCC 1 cut(s) 65
BclI TGATCA 1 cut(s) 912
BcoDI GTCTC 6 cut(s) 182, 359, 761, 924, 1313, 1444
BfmI CTRYAG 3 cut(s) 1266, 1309, 1345
BfuI GTATCC 1 cut(s) 65
BglI GCCNNNNNGGC 1 cut(s) 1175
BglII AGATCT 1 cut(s) 658
BisI GCNGC 5 cut(s) 38, 122, 474, 1008, 1425
BlsI GCNGC 5 cut(s) 39, 123, 475, 1009, 1426
Bme1390I CCNGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
Bme18I GGWCC 4 cut(s) 86, 260, 686, 1014
BmgT120I GGNCC 4 cut(s) 86, 260, 686, 1014
BmiI GGNNCC 6 cut(s) 87, 261, 439, 491, 688, 1015
BmrFI CCNGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 4 cut(s) 888, 1157, 1411, 1459
BmuI ACTGGG 1 cut(s) 809
BpiI GAAGAC 1 cut(s) 68
BplI GAGNNNNNCTC 6 cut(s) 356, 388, 758, 790, 1173, 1205
BpmI CTGGAG 1 cut(s) 1308
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 269
BsaI GGTCTC 3 cut(s) 182, 359, 761
BsaJI CCNNGG 6 cut(s) 163, 255, 396, 923, 1287, 1503
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 48, 476, 1242
Bse1I ACTGG 3 cut(s) 280, 815, 1277
Bse3DI GCAATG 1 cut(s) 509
BseBI CCWGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
BseDI CCNNGG 6 cut(s) 163, 255, 396, 923, 1287, 1503
BseGI GGATG 4 cut(s) 295, 605, 1148, 1426
BseLI CCNNNNNNNGG 4 cut(s) 47, 48, 476, 1242
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 5 cut(s) 116, 239, 255, 908, 1398
BseNI ACTGG 3 cut(s) 280, 815, 1277
BseRI GAGGAG 1 cut(s) 1462
BseXI GCAGC 4 cut(s) 24, 108, 1019, 1411
BseYI CCCAGC 2 cut(s) 514, 1010
BsgI GTGCAG 1 cut(s) 1026
Bsh1236I CGCG 1 cut(s) 934
Bsh1285I CGRYCG 1 cut(s) 101
BshFI GGCC 6 cut(s) 318, 395, 928, 1178, 1246, 1388
BshNI GGYRCC 2 cut(s) 437, 489
BsiEI CGRYCG 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 1220
BslFI GGGAC 3 cut(s) 273, 1057, 1319
BslI CCNNNNNNNGG 4 cut(s) 47, 48, 476, 1242
BsmAI GTCTC 6 cut(s) 182, 359, 761, 924, 1313, 1444
BsmFI GGGAC 3 cut(s) 273, 1057, 1319
BsnI GGCC 6 cut(s) 318, 395, 928, 1178, 1246, 1388
Bso31I GGTCTC 3 cut(s) 182, 359, 761
Bsp1286I GDGCHC 2 cut(s) 1133, 1220
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 5 cut(s) 205, 431, 473, 1118, 1221
BspANI GGCC 6 cut(s) 318, 395, 928, 1178, 1246, 1388
BspCNI CTCAG 5 cut(s) 117, 238, 256, 907, 1399
BspFNI CGCG 1 cut(s) 934
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 6 cut(s) 87, 261, 439, 491, 688, 1015
BspMAI CTGCAG 1 cut(s) 1313
BspPI GGATC 7 cut(s) 223, 353, 461, 564, 661, 1384, 1408
BspT107I GGYRCC 2 cut(s) 437, 489
BspTNI GGTCTC 3 cut(s) 182, 359, 761
BsrDI GCAATG 1 cut(s) 509
BsrI ACTGG 3 cut(s) 280, 815, 1277
BssECI CCNNGG 6 cut(s) 163, 255, 396, 923, 1287, 1503
BssSI CACGAG 2 cut(s) 789, 1206
BssT1I CCWWGG 2 cut(s) 163, 255
Bst2BI CACGAG 2 cut(s) 789, 1206
Bst2UI CCWGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
Bst4CI ACNGT 2 cut(s) 708, 1455
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstC8I GCNNGC 7 cut(s) 133, 148, 393, 875, 936, 1248, 1460
BstDEI CTNAG 6 cut(s) 125, 225, 264, 894, 992, 1407
BstDSI CCRYGG 4 cut(s) 163, 396, 923, 1287
BstF5I GGATG 4 cut(s) 295, 605, 1148, 1426
BstFNI CGCG 1 cut(s) 934
BstHHI GCGC 1 cut(s) 239
BstMAI GTCTC 6 cut(s) 182, 359, 761, 924, 1313, 1444
BstMCI CGRYCG 1 cut(s) 101
BstMWI GCNNNNNNNGC 3 cut(s) 1154, 1175, 1478
BstNI CCWGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
BstSCI CCNGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
BstSFI CTRYAG 3 cut(s) 1266, 1309, 1345
BstUI CGCG 1 cut(s) 934
BstV1I GCAGC 4 cut(s) 24, 108, 1019, 1411
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 4 cut(s) 228, 556, 658, 1376
BstYI RGATCY 4 cut(s) 228, 556, 658, 1376
BsuI GTATCC 1 cut(s) 65
BsuRI GGCC 6 cut(s) 318, 395, 928, 1178, 1246, 1388
BtgI CCRYGG 4 cut(s) 163, 396, 923, 1287
BtsCI GGATG 4 cut(s) 295, 605, 1148, 1426
BtsI GCAGTG 4 cut(s) 207, 504, 885, 1318
BtsIMutI CAGTG 7 cut(s) 207, 504, 566, 885, 975, 1318, 1404
Cac8I GCNNGC 7 cut(s) 133, 148, 393, 875, 936, 1248, 1460
CaiI CAGNNNCTG 1 cut(s) 476
CciI TCATGA 1 cut(s) 172
CfoI GCGC 1 cut(s) 239
Cfr13I GGNCC 4 cut(s) 86, 260, 686, 1014
Csp6I GTAC 3 cut(s) 78, 644, 1240
CviQI GTAC 3 cut(s) 78, 644, 1240
DdeI CTNAG 6 cut(s) 125, 225, 264, 894, 992, 1407
EaeI YGGCCR 2 cut(s) 393, 926
Eco130I CCWWGG 2 cut(s) 163, 255
Eco24I GRGCYC 1 cut(s) 1133
Eco31I GGTCTC 3 cut(s) 182, 359, 761
Eco47I GGWCC 4 cut(s) 86, 260, 686, 1014
Eco57I CTGAAG 3 cut(s) 50, 960, 1392
EcoO109I RGGNCCY 3 cut(s) 86, 260, 1014
EcoRII CCWGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
EcoT14I CCWWGG 2 cut(s) 163, 255
EcoT38I GRGCYC 1 cut(s) 1133
ErhI CCWWGG 2 cut(s) 163, 255
FaqI GGGAC 3 cut(s) 273, 1057, 1319
FbaI TGATCA 1 cut(s) 912
FblI GTMKAC 1 cut(s) 1112
Fnu4HI GCNGC 5 cut(s) 38, 122, 474, 1008, 1425
FokI GGATG 4 cut(s) 302, 612, 1135, 1433
FriOI GRGCYC 1 cut(s) 1133
Fsp4HI GCNGC 5 cut(s) 38, 122, 474, 1008, 1425
GlaI GCGC 1 cut(s) 238
GluI GCNGC 5 cut(s) 38, 122, 474, 1008, 1425
GsaI CCCAGC 2 cut(s) 518, 1014
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 6 cut(s) 318, 395, 928, 1178, 1246, 1388
HhaI GCGC 1 cut(s) 239
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 1 cut(s) 1203
HindII GTYRAC 1 cut(s) 1203
HphI GGTGA 6 cut(s) 319, 368, 530, 1010, 1423, 1478
Hpy166II GTNNAC 4 cut(s) 78, 400, 1113, 1203
Hpy188I TCNGA 6 cut(s) 228, 273, 658, 897, 1163, 1399
Hpy188III TCNNGA 4 cut(s) 58, 173, 362, 1022
Hpy8I GTNNAC 4 cut(s) 78, 400, 1113, 1203
HpyAV CCTTC 5 cut(s) 25, 99, 308, 571, 1367
HpyCH4III ACNGT 2 cut(s) 708, 1455
HpyCH4V TGCA 8 cut(s) 217, 509, 788, 1007, 1148, 1311, 1382, 1458
HpyF10VI GCNNNNNNNGC 3 cut(s) 1154, 1175, 1478
HpyF3I CTNAG 6 cut(s) 125, 225, 264, 894, 992, 1407
HspAI GCGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 912
LmnI GCTCC 2 cut(s) 34, 1215
Lsp1109I GCAGC 4 cut(s) 24, 108, 1019, 1411
LweI GCATC 4 cut(s) 888, 1157, 1411, 1459
MaeIII GTNAC 1 cut(s) 883
MboII GAAGA 6 cut(s) 73, 79, 530, 551, 599, 1048
MflI RGATCY 4 cut(s) 228, 556, 658, 1376
MhlI GDGCHC 2 cut(s) 1133, 1220
MluCI AATT 2 cut(s) 825, 1226
MluI ACGCGT 1 cut(s) 932
MlyI GAGTC 5 cut(s) 767, 1150, 1198, 1286, 1315
MmeI TCCRAC 1 cut(s) 93
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 6 cut(s) 153, 309, 450, 666, 1095, 1512
MslI CAYNNNNRTG 6 cut(s) 212, 374, 623, 896, 1196, 1409
MspA1I CMGCKG 3 cut(s) 473, 1010, 1250
MspR9I CCNGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
MvaI CCWGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
MvnI CGCG 1 cut(s) 934
MwoI GCNNNNNNNGC 3 cut(s) 1154, 1175, 1478
NcoI CCATGG 1 cut(s) 163
NlaIV GGNNCC 6 cut(s) 87, 261, 439, 491, 688, 1015
NmuCI GTSAC 1 cut(s) 883
OliI CACNNNNGTG 1 cut(s) 1409
PagI TCATGA 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 931
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 7 cut(s) 268, 279, 905, 1067, 1357, 1394, 1400
PflMI CCANNNNNTGG 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 1416
PkrI GCNGC 5 cut(s) 39, 123, 475, 1009, 1426
PleI GAGTC 5 cut(s) 767, 1149, 1198, 1286, 1315
PpsI GAGTC 5 cut(s) 767, 1149, 1198, 1286, 1315
PpuMI RGGWCCY 3 cut(s) 86, 260, 1014
Psp5II RGGWCCY 3 cut(s) 86, 260, 1014
Psp6I CCWGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
PspFI CCCAGC 2 cut(s) 514, 1010
PspGI CCWGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
PspN4I GGNNCC 6 cut(s) 87, 261, 439, 491, 688, 1015
PspPI GGNCC 4 cut(s) 86, 260, 686, 1014
PspPPI RGGWCCY 3 cut(s) 86, 260, 1014
PstI CTGCAG 1 cut(s) 1313
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 4 cut(s) 228, 556, 658, 1376
PvuII CAGCTG 2 cut(s) 1010, 1250
RsaI GTAC 3 cut(s) 79, 645, 1241
RsaNI GTAC 3 cut(s) 78, 644, 1240
RseI CAYNNNNRTG 6 cut(s) 212, 374, 623, 896, 1196, 1409
SaqAI TTAA 6 cut(s) 153, 309, 450, 666, 1095, 1512
SatI GCNGC 5 cut(s) 38, 122, 474, 1008, 1425
Sau96I GGNCC 4 cut(s) 86, 260, 686, 1014
SchI GAGTC 5 cut(s) 767, 1150, 1198, 1286, 1315
ScrFI CCNGG 7 cut(s) 185, 320, 411, 543, 731, 1418, 1464
SduI GDGCHC 2 cut(s) 1133, 1220
SfaNI GCATC 4 cut(s) 888, 1157, 1411, 1459
SfcI CTRYAG 3 cut(s) 1266, 1309, 1345
SinI GGWCC 4 cut(s) 86, 260, 686, 1014
SmiMI CAYNNNNRTG 6 cut(s) 212, 374, 623, 896, 1196, 1409
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 2 cut(s) 825, 1226
SsiI CCGC 5 cut(s) 205, 431, 473, 1118, 1221
StyD4I CCNGG 7 cut(s) 183, 318, 409, 541, 729, 1416, 1462
StyI CCWWGG 2 cut(s) 163, 255
TaaI ACNGT 2 cut(s) 708, 1455
TaqI TCGA 2 cut(s) 249, 1048
TaqII GACCGA 3 cut(s) 87, 785, 1495
TasI AATT 2 cut(s) 825, 1226
TatI WGTACW 2 cut(s) 77, 1239
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 7 cut(s) 268, 279, 905, 1067, 1357, 1394, 1400
Tru1I TTAA 6 cut(s) 153, 309, 450, 666, 1095, 1512
Tru9I TTAA 6 cut(s) 153, 309, 450, 666, 1095, 1512
TscAI CASTG 7 cut(s) 214, 511, 573, 892, 982, 1318, 1411
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 4 cut(s) 37, 121, 1007, 1424
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 5 cut(s) 81, 152, 627, 769, 1475
TspGWI ACGGA 2 cut(s) 662, 1304
TspRI CASTG 7 cut(s) 214, 511, 573, 892, 982, 1318, 1411
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 4 cut(s) 86, 260, 686, 1014
XapI RAATTY 1 cut(s) 825
XmiI GTMKAC 1 cut(s) 1112
XmnI GAANNNNTTC 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.