RLG00000005595

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009829
Physical Location & Seq
Reverse (-)
166743 .. 170925
4183 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005595

Sequence Viewer

Length: 1665 bp
ATGAGAAGAGTATGTCACTTGGCCTGTTCTCTTCCTGCTCTATTTCAGACTACCACGAGGTCGAAGCTCGCTACTGTGTACTTACGCCTTGGCGAATTGAATCGAAATTTAGTACATCTTGAAGATCGATATATGGCGAACATAGACATAGAGGGAATACTGAAGGAGCTGCCCAACGATGGGCGTATCCCGAAGACAAAGATCGTGTGCACTTTGGGTCCTTCATCTCGGTCGGTGGAGATGTTGGAGAAGCTGCTGAGGGCTGGCATGAATGTTGCTCGCTTTAACTTCTCCCATGGCACTCACGACTACCACCAGGAGACCCTCAACAATCTCCGCACTGCCATGCACAACACTCAGATCCTCTGCGCCGTCATGCTTGATACCAAGGGACCTGAGATTCGGACTGGATTCCTCAAGGATGGGAAGCCTATTCAACTTAAAGAAGGCCAGGAAATCACCATTACTACTGACTATAGCATCAAGGGTGATCCAGAGACCATCTCCATGAGCTACAAGAAGCTGGCCGTGGACTTGAAGCCCGGAAATACCATTTTATGTGCAGACGGCACCATTACCCTTGCTGTGTTGTCTTGTGATCCAGCGGCTGGTATTGTGAGGTGCCGTTGTGAGAACACTGCAATGCTGGGTGAGAGGAAAAATGTCAATCTTCCTGGTGTTGTGGTGGATCTACCCACACTGACAGAGAAGGACAAGGAAGATATTCTGGGATGGGGTGTTCCCAACAACATTGATATGATTGCTCTTTCATTTGTACGCAAGGGATCAGATCTTGTTAATGTCCGTAAAGTTCTTGGACCCCATGCCAAGAACATACAGTTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAACTTTGATGAGATACTGCGTGAGACCGACTCATTCATGGTTGCACGAGGCGATCTTGGTATGGAAATCCCAGTTGAGAAAATTTTTCTGGCACAAAAAATGATGATATATAAGTGTAATCTTGTGGGCAAGCCTGTTGTCACTGCCACTCAGATGCTTGAATCCATGATCAAGTCTCCACGGCCAACGCGTGCTGAAGCCACAGATGTAGCTAATGCTGTCCTTGATGGCACTGATTGTGTCATGCTTAGTGGGGAGAGTGCAGCTGGGTCCTATCCTGAACTCGCTGTGAAGATCATGGCTCGAATATGTATTGAGGCAGAATCGTCCCTTGACTATGGGGCTATCTTTAAGGAGATGATAAAGTCTACACCGCTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCTGCTGTCCGAACAGCCAACAAGGCCAGAGCCAAACTCATTGTTGTGTTGACTCGTGGTGGGAGCACCGCAAAATTGGTAGCCAAGTACAGGCCAGCTGTTCCTATCCTATCTGTAGTTGTTCCAGTTTTGACCACGGACTCATTTGATTGGGACTGCAGTGATGAGACTCCAGCAAGACATAGCCTGATCTACAGGGGTTTGATTCCTCTATTGGCTGAAGGATCTGCAAAGGCCACCGATTCTGAATCCACTGAGGTGATCCTGGATGCTGCTCTAAAGTCGGCAACAAAGAGGAGACTGTGCAAGCCTGGTGATGCTGTGGTGGCACTTCATCGTATTGGAGTTGCCTCTGTCATTAAGATTTGCATAGTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

555

Amino Acids

60.43

Weight (kDa)

8.52

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 64 - 389 2.2e-152 Pyruvate kinase, barrel domain
PK_C PF02887 424 - 544 4.9e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009829)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08570 AT5G08570 AT5G08570 AT5G63680 AT5G63680 AT5G63680
fragaria_vesca FvH4_4g37360
malus_domestica MD13G1000500.v1.1 MD16G1005100.v1.1
prunus_persica Prupe.4G287000_v2.0.a1
pyrus_communis pycom2749g00020
rosa_chinensis RchiOBHm_Chr4g0447231
rosa_laevigata RLG00000005595
rosa_multiflora Rmu_ssc0000396.1_g000002
rosa_roxburghii Rroxscaffold_5G00387590
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4AG440400 Rh4BG449400 Rh4CG466400 Rh4DG447900
rosa_wichuraiana Rw4G037680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 569, 621
AccB7I CCANNNNNTGG 1 cut(s) 608
AccI GTMKAC 1 cut(s) 1244
AccII CGCG 1 cut(s) 1066
AciI CCGC 4 cut(s) 337, 605, 1250, 1353
AclWI GGATC 7 cut(s) 355, 485, 593, 696, 793, 1516, 1540
AcoI YGGCCR 2 cut(s) 525, 1058
AcsI RAATTY 2 cut(s) 106, 957
AcuI CTGAAG 3 cut(s) 182, 1092, 1524
AfaI GTAC 4 cut(s) 80, 114, 777, 1373
AfiI CCNNNNNNNGG 4 cut(s) 179, 180, 608, 1374
AflIII ACRYGT 1 cut(s) 1064
AgsI TTSAA 5 cut(s) 100, 122, 437, 538, 1037
AjnI CCWGG 6 cut(s) 315, 450, 673, 861, 1548, 1594
AleI CACNNNNGTG 1 cut(s) 1541
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
AluBI AGCT 8 cut(s) 67, 169, 253, 513, 523, 1088, 1142, 1382
AluI AGCT 8 cut(s) 67, 169, 253, 513, 523, 1088, 1142, 1382
Alw21I GWGCWC 2 cut(s) 212, 1352
Alw26I GTCTC 6 cut(s) 314, 491, 893, 1056, 1445, 1576
Alw44I GTGCAC 1 cut(s) 208
AlwI GGATC 7 cut(s) 355, 485, 593, 696, 793, 1516, 1540
AlwNI CAGNNNCTG 1 cut(s) 608
AoxI GGCC 7 cut(s) 21, 448, 525, 1058, 1308, 1376, 1518
ApaLI GTGCAC 1 cut(s) 208
ApeKI GCWGC 4 cut(s) 169, 253, 1139, 1556
ApoI RAATTY 2 cut(s) 106, 957
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
Asp700I GAANNNNTTC 1 cut(s) 723
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 4 cut(s) 218, 392, 818, 1146
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 5 cut(s) 451, 500, 662, 1555, 1610
AvaII GGWCC 4 cut(s) 218, 392, 818, 1146
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 2 cut(s) 569, 621
BanII GRGCYC 1 cut(s) 1265
BauI CACGAG 3 cut(s) 55, 921, 1338
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 212, 1352
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 156, 240, 1151, 1543
BccI CCATC 5 cut(s) 173, 416, 509, 726, 1097
BceAI ACGGC 5 cut(s) 356, 512, 583, 609, 1073
BciT130I CCWGG 6 cut(s) 317, 452, 675, 863, 1550, 1596
BciVI GTATCC 1 cut(s) 197
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 6 cut(s) 314, 491, 893, 1056, 1445, 1576
BfmI CTRYAG 4 cut(s) 475, 1398, 1441, 1477
BfuI GTATCC 1 cut(s) 197
BglI GCCNNNNNGGC 1 cut(s) 1307
BglII AGATCT 1 cut(s) 790
BisI GCNGC 5 cut(s) 170, 254, 606, 1140, 1557
BlsI GCNGC 5 cut(s) 171, 255, 607, 1141, 1558
Bme1390I CCNGG 7 cut(s) 317, 452, 543, 675, 863, 1550, 1596
Bme18I GGWCC 4 cut(s) 218, 392, 818, 1146
BmgT120I GGNCC 4 cut(s) 218, 392, 818, 1146
BmiI GGNNCC 6 cut(s) 219, 393, 571, 623, 820, 1147
BmrFI CCNGG 7 cut(s) 317, 452, 543, 675, 863, 1550, 1596
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 5 cut(s) 489, 1020, 1289, 1543, 1591
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 200
BplI GAGNNNNNCTC 6 cut(s) 488, 520, 890, 922, 1305, 1337
BpmI CTGGAG 1 cut(s) 1440
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 127
BsaI GGTCTC 3 cut(s) 314, 491, 893
BsaJI CCNNGG 6 cut(s) 88, 295, 387, 528, 1055, 1419
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 180, 608, 1374
Bse1I ACTGG 3 cut(s) 412, 947, 1409
Bse3DI GCAATG 1 cut(s) 648
BseBI CCWGG 6 cut(s) 317, 452, 675, 863, 1550, 1596
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 6 cut(s) 88, 295, 387, 528, 1055, 1419
BseGI GGATG 4 cut(s) 427, 737, 1280, 1558
BseLI CCNNNNNNNGG 4 cut(s) 179, 180, 608, 1374
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 5 cut(s) 248, 371, 387, 1040, 1530
BseNI ACTGG 3 cut(s) 412, 947, 1409
BseRI GAGGAG 1 cut(s) 1594
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 4 cut(s) 156, 240, 1151, 1543
BseYI CCCAGC 2 cut(s) 646, 1142
BsgI GTGCAG 2 cut(s) 582, 1158
Bsh1236I CGCG 1 cut(s) 1066
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 7 cut(s) 23, 450, 527, 1060, 1310, 1378, 1520
BshNI GGYRCC 2 cut(s) 569, 621
BshVI ATCGAT 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 233
BsiHKAI GWGCWC 2 cut(s) 212, 1352
BsiSI CCGG 1 cut(s) 543
BslFI GGGAC 3 cut(s) 405, 1189, 1451
BslI CCNNNNNNNGG 4 cut(s) 179, 180, 608, 1374
BsmAI GTCTC 6 cut(s) 314, 491, 893, 1056, 1445, 1576
BsmFI GGGAC 3 cut(s) 405, 1189, 1451
BsnI GGCC 7 cut(s) 23, 450, 527, 1060, 1310, 1378, 1520
Bso31I GGTCTC 3 cut(s) 314, 491, 893
Bsp1286I GDGCHC 3 cut(s) 212, 1265, 1352
Bsp19I CCATGG 1 cut(s) 295
BspACI CCGC 4 cut(s) 337, 605, 1250, 1353
BspANI GGCC 7 cut(s) 23, 450, 527, 1060, 1310, 1378, 1520
BspCNI CTCAG 5 cut(s) 249, 370, 388, 1039, 1531
BspDI ATCGAT 1 cut(s) 127
BspFNI CGCG 1 cut(s) 1066
BspLI GGNNCC 6 cut(s) 219, 393, 571, 623, 820, 1147
BspMAI CTGCAG 1 cut(s) 1445
BspPI GGATC 7 cut(s) 355, 485, 593, 696, 793, 1516, 1540
BspT107I GGYRCC 2 cut(s) 569, 621
BspTNI GGTCTC 3 cut(s) 314, 491, 893
BsrDI GCAATG 1 cut(s) 648
BsrI ACTGG 3 cut(s) 412, 947, 1409
BssECI CCNNGG 6 cut(s) 88, 295, 387, 528, 1055, 1419
BssSI CACGAG 3 cut(s) 55, 921, 1338
BssT1I CCWWGG 3 cut(s) 88, 295, 387
Bst2BI CACGAG 3 cut(s) 55, 921, 1338
Bst2UI CCWGG 6 cut(s) 317, 452, 675, 863, 1550, 1596
Bst4CI ACNGT 3 cut(s) 76, 840, 1587
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstC8I GCNNGC 8 cut(s) 69, 265, 280, 525, 1007, 1068, 1380, 1592
BstDEI CTNAG 6 cut(s) 257, 357, 396, 1026, 1124, 1539
BstDSI CCRYGG 4 cut(s) 295, 528, 1055, 1419
BstF5I GGATG 4 cut(s) 427, 737, 1280, 1558
BstFNI CGCG 1 cut(s) 1066
BstHHI GCGC 1 cut(s) 371
BstMAI GTCTC 6 cut(s) 314, 491, 893, 1056, 1445, 1576
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 3 cut(s) 1286, 1307, 1610
BstNI CCWGG 6 cut(s) 317, 452, 675, 863, 1550, 1596
BstSCI CCNGG 7 cut(s) 315, 450, 541, 673, 861, 1548, 1594
BstSFI CTRYAG 4 cut(s) 475, 1398, 1441, 1477
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 1 cut(s) 1066
BstV1I GCAGC 4 cut(s) 156, 240, 1151, 1543
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 4 cut(s) 360, 688, 790, 1508
BstYI RGATCY 4 cut(s) 360, 688, 790, 1508
Bsu15I ATCGAT 1 cut(s) 127
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 7 cut(s) 23, 450, 527, 1060, 1310, 1378, 1520
BsuTUI ATCGAT 1 cut(s) 127
BtgI CCRYGG 4 cut(s) 295, 528, 1055, 1419
BtsCI GGATG 4 cut(s) 427, 737, 1280, 1558
BtsI GCAGTG 4 cut(s) 339, 636, 1017, 1450
BtsIMutI CAGTG 7 cut(s) 339, 636, 698, 1017, 1107, 1450, 1536
Cac8I GCNNGC 8 cut(s) 69, 265, 280, 525, 1007, 1068, 1380, 1592
CaiI CAGNNNCTG 1 cut(s) 608
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 4 cut(s) 218, 392, 818, 1146
ClaI ATCGAT 1 cut(s) 127
Csp6I GTAC 4 cut(s) 79, 113, 776, 1372
CviQI GTAC 4 cut(s) 79, 113, 776, 1372
DdeI CTNAG 6 cut(s) 257, 357, 396, 1026, 1124, 1539
EaeI YGGCCR 2 cut(s) 525, 1058
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 88, 295, 387
Eco24I GRGCYC 1 cut(s) 1265
Eco31I GGTCTC 3 cut(s) 314, 491, 893
Eco47I GGWCC 4 cut(s) 218, 392, 818, 1146
Eco57I CTGAAG 3 cut(s) 182, 1092, 1524
EcoO109I RGGNCCY 3 cut(s) 218, 392, 1146
EcoRII CCWGG 6 cut(s) 315, 450, 673, 861, 1548, 1594
EcoT14I CCWWGG 3 cut(s) 88, 295, 387
EcoT38I GRGCYC 1 cut(s) 1265
ErhI CCWWGG 3 cut(s) 88, 295, 387
FaqI GGGAC 3 cut(s) 405, 1189, 1451
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 1 cut(s) 1244
Fnu4HI GCNGC 5 cut(s) 170, 254, 606, 1140, 1557
FokI GGATG 4 cut(s) 434, 744, 1267, 1565
FriOI GRGCYC 1 cut(s) 1265
Fsp4HI GCNGC 5 cut(s) 170, 254, 606, 1140, 1557
GlaI GCGC 1 cut(s) 370
GluI GCNGC 5 cut(s) 170, 254, 606, 1140, 1557
GsaI CCCAGC 2 cut(s) 650, 1146
GsuI CTGGAG 1 cut(s) 1440
HaeIII GGCC 7 cut(s) 23, 450, 527, 1060, 1310, 1378, 1520
HapII CCGG 1 cut(s) 543
HhaI GCGC 1 cut(s) 371
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 1 cut(s) 1335
HindII GTYRAC 1 cut(s) 1335
HpaII CCGG 1 cut(s) 543
HphI GGTGA 5 cut(s) 451, 500, 662, 1555, 1610
Hpy166II GTNNAC 5 cut(s) 79, 210, 532, 1245, 1335
Hpy188I TCNGA 7 cut(s) 48, 360, 405, 790, 1029, 1295, 1531
Hpy188III TCNNGA 5 cut(s) 119, 190, 305, 494, 1154
Hpy8I GTNNAC 5 cut(s) 79, 210, 532, 1245, 1335
HpyAV CCTTC 5 cut(s) 157, 231, 440, 703, 1499
HpyCH4III ACNGT 3 cut(s) 76, 840, 1587
HpyF10VI GCNNNNNNNGC 3 cut(s) 1286, 1307, 1610
HpyF3I CTNAG 6 cut(s) 257, 357, 396, 1026, 1124, 1539
HspAI GCGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 1044
LmnI GCTCC 2 cut(s) 166, 1347
Lsp1109I GCAGC 4 cut(s) 156, 240, 1151, 1543
LweI GCATC 5 cut(s) 489, 1020, 1289, 1543, 1591
MaeIII GTNAC 2 cut(s) 14, 1015
MboII GAAGA 7 cut(s) 18, 23, 134, 205, 662, 731, 1180
MflI RGATCY 4 cut(s) 360, 688, 790, 1508
MhlI GDGCHC 3 cut(s) 212, 1265, 1352
MluCI AATT 4 cut(s) 95, 106, 957, 1358
MluI ACGCGT 1 cut(s) 1064
MlyI GAGTC 5 cut(s) 899, 1282, 1330, 1418, 1447
MmeI TCCRAC 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 723
MseI TTAA 5 cut(s) 285, 441, 798, 1227, 1644
MslI CAYNNNNRTG 7 cut(s) 344, 506, 641, 755, 1028, 1328, 1541
MspA1I CMGCKG 3 cut(s) 605, 1142, 1382
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 7 cut(s) 317, 452, 543, 675, 863, 1550, 1596
MvaI CCWGG 6 cut(s) 317, 452, 675, 863, 1550, 1596
MvnI CGCG 1 cut(s) 1066
MwoI GCNNNNNNNGC 3 cut(s) 1286, 1307, 1610
NciI CCSGG 1 cut(s) 543
NcoI CCATGG 1 cut(s) 295
NlaIV GGNNCC 6 cut(s) 219, 393, 571, 623, 820, 1147
NmuCI GTSAC 2 cut(s) 14, 1015
OliI CACNNNNGTG 1 cut(s) 1541
PcsI WCGNNNNNNNCGW 1 cut(s) 1063
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 8 cut(s) 100, 400, 411, 1037, 1199, 1489, 1526, 1532
PflMI CCANNNNNTGG 1 cut(s) 608
PfoI TCCNGGA 1 cut(s) 1548
PkrI GCNGC 5 cut(s) 171, 255, 607, 1141, 1558
PleI GAGTC 5 cut(s) 899, 1281, 1330, 1418, 1447
PpsI GAGTC 5 cut(s) 899, 1281, 1330, 1418, 1447
PpuMI RGGWCCY 3 cut(s) 218, 392, 1146
Psp5II RGGWCCY 3 cut(s) 218, 392, 1146
Psp6I CCWGG 6 cut(s) 315, 450, 673, 861, 1548, 1594
PspFI CCCAGC 2 cut(s) 646, 1142
PspGI CCWGG 6 cut(s) 315, 450, 673, 861, 1548, 1594
PspN4I GGNNCC 6 cut(s) 219, 393, 571, 623, 820, 1147
PspPI GGNCC 4 cut(s) 218, 392, 818, 1146
PspPPI RGGWCCY 3 cut(s) 218, 392, 1146
PstI CTGCAG 1 cut(s) 1445
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 4 cut(s) 360, 688, 790, 1508
PvuII CAGCTG 2 cut(s) 1142, 1382
RsaI GTAC 4 cut(s) 80, 114, 777, 1373
RsaNI GTAC 4 cut(s) 79, 113, 776, 1372
RseI CAYNNNNRTG 7 cut(s) 344, 506, 641, 755, 1028, 1328, 1541
SaqAI TTAA 5 cut(s) 285, 441, 798, 1227, 1644
SatI GCNGC 5 cut(s) 170, 254, 606, 1140, 1557
Sau96I GGNCC 4 cut(s) 218, 392, 818, 1146
SchI GAGTC 5 cut(s) 899, 1282, 1330, 1418, 1447
ScrFI CCNGG 7 cut(s) 317, 452, 543, 675, 863, 1550, 1596
SduI GDGCHC 3 cut(s) 212, 1265, 1352
SfaNI GCATC 5 cut(s) 489, 1020, 1289, 1543, 1591
SfcI CTRYAG 4 cut(s) 475, 1398, 1441, 1477
SinI GGWCC 4 cut(s) 218, 392, 818, 1146
SmiMI CAYNNNNRTG 7 cut(s) 344, 506, 641, 755, 1028, 1328, 1541
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 4 cut(s) 95, 106, 957, 1358
SsiI CCGC 4 cut(s) 337, 605, 1250, 1353
StyD4I CCNGG 7 cut(s) 315, 450, 541, 673, 861, 1548, 1594
StyI CCWWGG 3 cut(s) 88, 295, 387
TaaI ACNGT 3 cut(s) 76, 840, 1587
TaqI TCGA 4 cut(s) 62, 103, 127, 1180
TaqII GACCGA 2 cut(s) 219, 917
TasI AATT 4 cut(s) 95, 106, 957, 1358
TatI WGTACW 3 cut(s) 78, 112, 1371
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 8 cut(s) 100, 400, 411, 1037, 1199, 1489, 1526, 1532
Tru1I TTAA 5 cut(s) 285, 441, 798, 1227, 1644
Tru9I TTAA 5 cut(s) 285, 441, 798, 1227, 1644
TscAI CASTG 7 cut(s) 346, 643, 705, 1024, 1114, 1450, 1543
TseFI GTSAC 2 cut(s) 14, 1015
TseI GCWGC 4 cut(s) 169, 253, 1139, 1556
Tsp45I GTSAC 2 cut(s) 14, 1015
TspDTI ATGAA 5 cut(s) 213, 284, 759, 901, 1607
TspGWI ACGGA 2 cut(s) 794, 1436
TspRI CASTG 7 cut(s) 346, 643, 705, 1024, 1114, 1450, 1543
Van91I CCANNNNNTGG 1 cut(s) 608
VneI GTGCAC 1 cut(s) 208
VpaK11BI GGWCC 4 cut(s) 218, 392, 818, 1146
XapI RAATTY 2 cut(s) 106, 957
XmiI GTMKAC 1 cut(s) 1244
XmnI GAANNNNTTC 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.