FvH4_5g01670

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008078
Physical Location & Seq
Forward (+)
1073323 .. 1078854
5532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01670.t5

Sequence Viewer

Length: 1476 bp
ATGGCAGTTAAACTGGAACAAGAGCCAATCACACCAAGGACGGCCACCGGTTGCACCAGTGCCACAGATTGCATCATCTGTTTCCAATCTGGTGCTTATGCCTCCAAGCTGCTATCGAGAAATGATAAAAACGGATGCAATCCAAGCGAGGTTAACAAGGCAACAGCAGACACTGGAAATGCTAACTTCGAAAAAGCTGGCGCACTAGCAGCAAAGGTCTTGGCAAAGTTGAACATTAAAGGAGCCAACAAAGGTGGAAAAGAAGATCATGAAATATCTCAAAATAATGGCGGAAGTGCTAGTCTTTTTAGCAATGAAGTGACAAAGCAGCCTAATTCCCATCGATATCCAAACACATCAAGTGCATATGGGCATAAGAATCCAATTGATTTAGATGAGAGTTCTGTCTTAGCATATTCAAGGGAGTGCAACGAGTGTTTTGGGTTGCATAGACCAAATCATCTAGTGCCAATATTACGCTATGCCGATGATCCGGAGCCTGAAGAAGGCAACTTGAATAATTCAGAGGCAGAAGATACAATGCAAAGACAAACAGATGGTAAACCAAGTTCAGCAGACAACACTCACAGCCCCAGTAGTTCATCAAGCAAAGGGGATAATGATTCTACTTCCATAGGAGGTTGTGAGATTCGTTGGGAGGACTTACATTTGGGAGAGGGAATCGGACAAGGTTCATGTGGTATTGTATATCGTGGAATCTGGAATGGATCGGATGTTGCCATTAAGGTTTACTTTAAAAATGAATACAGTGAGGGGATTTTACATGGCTACAGAAAGGAGATTGATATAATGAAAAGATTACGGCATCCTAATGTGCTGCTATTTATGGGAGCAGTATATTCACAAGAACGTCTTGCTATTGTCACAGAGTACTTACCTAGGGGTAGTCTTTTCAGACAACTTCACAAAAATAATCAAGCATTGGATATTAAGCGACGTATGAGGATGGCCCTTGATGTTGCGAGAGGTATGAATTACTTGCATCGTAGAAATCCACCTATAGTCCACAGAGACTTAAAATCGTCTAACTTGCTGGTTGACAAGAACTGGACTGTCAAGGTTGGAGACTTTGGTCTGTCGAAGTTCAAGAACGCAACCTTCTTGACTGCAAAATCTGGTAGAGGGACACCTCAATGGATGGCACCTGAGGTCCTCCGAAATGAACCTTCAAATGAGAAGTCTGATGTATTCAGCTATGGTGTCATCCTTTGGGAAATAATGACTGTATCGGTCCCATGGGATAACCTCAATCCCTTGCAGGTTGTTGGAGTTGTCGGCTTCATGGATAGGAGATTGGACATACCGGAAGGCCTCAATCCTGAAATAGTATCCATTATCGAAGACTGTTGGCGAAGTGACCCCGGCCAGCGCCCATCGTTTGAAGATGTGATTCAGATAATGAAGAACTTAGTTATCAAAGTTGAAGCAGCTTCAGTAAGAAGTTCAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

492

Amino Acids

54.38

Weight (kDa)

6.65

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 76 - 181 2e-09 PAS fold
PAS_4 PF08448 80 - 186 5.1e-07 PAS fold
PAS_9 PF13426 91 - 186 1.2e-08 PAS domain
PK_Tyr_Ser-Thr PF07714 428 - 678 1.5e-65 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 430 - 678 1.1e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1270
AccB1I GGYRCC 1 cut(s) 1162
AccIII TCCGGA 1 cut(s) 493
AciI CCGC 1 cut(s) 291
AclWI GGATC 2 cut(s) 485, 736
AcoI YGGCCR 2 cut(s) 42, 1384
AcuI CTGAAG 2 cut(s) 522, 1437
AfaI GTAC 1 cut(s) 893
AfiI CCNNNNNNNGG 1 cut(s) 506
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 8 cut(s) 232, 420, 517, 1108, 1191, 1403, 1445, 1467
AluBI AGCT 4 cut(s) 109, 197, 1215, 1451
AluI AGCT 4 cut(s) 109, 197, 1215, 1451
Alw26I GTCTC 2 cut(s) 1026, 1080
AlwI GGATC 2 cut(s) 485, 736
AlwNI CAGNNNCTG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 493
AoxI GGCC 4 cut(s) 42, 969, 1330, 1384
ApeKI GCWGC 5 cut(s) 109, 209, 328, 838, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 1184, 1216
AsiGI ACCGGT 1 cut(s) 47
AspA2I CCTAGG 1 cut(s) 899
AspLEI GCGC 2 cut(s) 203, 1392
AspS9I GGNCC 3 cut(s) 970, 1171, 1252
AsuC2I CCSGG 1 cut(s) 1383
AsuII TTCGAA 1 cut(s) 189
AvaII GGWCC 2 cut(s) 1171, 1252
AvrII CCTAGG 1 cut(s) 899
AxyI CCTNAGG 1 cut(s) 1167
BanI GGYRCC 1 cut(s) 1162
BbsI GAAGAC 1 cut(s) 1368
BbvI GCAGC 5 cut(s) 96, 221, 340, 825, 1460
BccI CCATC 5 cut(s) 348, 551, 961, 1153, 1402
BceAI ACGGC 2 cut(s) 57, 839
BciVI GTATCC 1 cut(s) 1360
BcnI CCSGG 1 cut(s) 1383
BcoDI GTCTC 2 cut(s) 1026, 1080
BfaI CTAG 4 cut(s) 206, 300, 464, 900
BfmI CTRYAG 2 cut(s) 790, 1020
BfoI RGCGCY 1 cut(s) 1393
BfuAI ACCTGC 1 cut(s) 1270
BfuI GTATCC 1 cut(s) 1360
BisI GCNGC 5 cut(s) 110, 210, 329, 839, 1449
BlnI CCTAGG 1 cut(s) 899
BlsI GCNGC 5 cut(s) 111, 211, 330, 840, 1450
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 1 cut(s) 1383
Bme18I GGWCC 2 cut(s) 1171, 1252
BmgT120I GGNCC 3 cut(s) 970, 1171, 1252
BmiI GGNNCC 4 cut(s) 244, 498, 1164, 1254
BmrFI CCNGG 1 cut(s) 1383
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 4 cut(s) 81, 125, 835, 1012
BmuI ACTGGG 1 cut(s) 588
BoxI GACNNNNGTC 1 cut(s) 1092
BpiI GAAGAC 1 cut(s) 1368
Bpu14I TTCGAA 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 1383
Bsa29I ATCGAT 1 cut(s) 343
BsaJI CCNNGG 4 cut(s) 35, 899, 1256, 1381
BsaWI WCCGGW 3 cut(s) 47, 493, 1324
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 5 cut(s) 18, 57, 178, 594, 1073
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 493
BseCI ATCGAT 1 cut(s) 343
BseDI CCNNGG 4 cut(s) 35, 899, 1256, 1381
BseGI GGATG 6 cut(s) 140, 739, 826, 972, 1164, 1224
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 1158
BseNI ACTGG 5 cut(s) 18, 57, 178, 594, 1073
BseXI GCAGC 5 cut(s) 96, 221, 340, 825, 1460
BshFI GGCC 4 cut(s) 44, 971, 1332, 1386
BshNI GGYRCC 1 cut(s) 1162
BshTI ACCGGT 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 343
BsiSI CCGG 4 cut(s) 48, 494, 1325, 1383
BslFI GGGAC 2 cut(s) 1159, 1238
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 2 cut(s) 1026, 1080
BsmFI GGGAC 2 cut(s) 1159, 1238
BsnI GGCC 4 cut(s) 44, 971, 1332, 1386
Bsp119I TTCGAA 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 493
Bsp143I GATC 3 cut(s) 265, 490, 728
Bsp19I CCATGG 1 cut(s) 1256
BspACI CCGC 1 cut(s) 291
BspANI GGCC 4 cut(s) 44, 971, 1332, 1386
BspCNI CTCAG 1 cut(s) 1159
BspDI ATCGAT 1 cut(s) 343
BspEI TCCGGA 1 cut(s) 493
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 4 cut(s) 244, 498, 1164, 1254
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 2 cut(s) 485, 736
BspT104I TTCGAA 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 1162
BsrDI GCAATG 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 5 cut(s) 18, 57, 178, 594, 1073
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 4 cut(s) 35, 899, 1256, 1381
BssMI GATC 3 cut(s) 265, 490, 728
BssT1I CCWWGG 3 cut(s) 35, 899, 1256
Bst4CI ACNGT 4 cut(s) 770, 1075, 1246, 1367
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 199, 1388
BstDEI CTNAG 3 cut(s) 409, 1167, 1429
BstDSI CCRYGG 1 cut(s) 1256
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 6 cut(s) 140, 739, 826, 972, 1164, 1224
BstH2I RGCGCY 1 cut(s) 1393
BstHHI GCGC 2 cut(s) 203, 1392
BstKTI GATC 3 cut(s) 268, 493, 731
BstMAI GTCTC 2 cut(s) 1026, 1080
BstMBI GATC 3 cut(s) 265, 490, 728
BstMWI GCNNNNNNNGC 2 cut(s) 144, 209
BstPAI GACNNNNGTC 1 cut(s) 1092
BstSCI CCNGG 1 cut(s) 1381
BstSFI CTRYAG 2 cut(s) 790, 1020
BstV1I GCAGC 5 cut(s) 96, 221, 340, 825, 1460
BstV2I GAAGAC 1 cut(s) 1368
Bsu15I ATCGAT 1 cut(s) 343
Bsu36I CCTNAGG 1 cut(s) 1167
BsuI GTATCC 1 cut(s) 1360
BsuRI GGCC 4 cut(s) 44, 971, 1332, 1386
BsuTUI ATCGAT 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 1256
BtsCI GGATG 6 cut(s) 140, 739, 826, 972, 1164, 1224
BtsIMutI CAGTG 3 cut(s) 64, 171, 775
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 2 cut(s) 199, 1388
CaiI CAGNNNCTG 1 cut(s) 173
CciI TCATGA 1 cut(s) 268
CfoI GCGC 2 cut(s) 203, 1392
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 3 cut(s) 970, 1171, 1252
ClaI ATCGAT 1 cut(s) 343
Csp6I GTAC 1 cut(s) 892
CspAI ACCGGT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 5 cut(s) 269, 696, 785, 1257, 1303
CviQI GTAC 1 cut(s) 892
DdeI CTNAG 3 cut(s) 409, 1167, 1429
DpnI GATC 3 cut(s) 267, 492, 730
DpnII GATC 3 cut(s) 265, 490, 728
DraI TTTAAA 1 cut(s) 757
EaeI YGGCCR 2 cut(s) 42, 1384
EciI GGCGGA 1 cut(s) 306
Eco130I CCWWGG 3 cut(s) 35, 899, 1256
Eco147I AGGCCT 1 cut(s) 1332
Eco32I GATATC 1 cut(s) 347
Eco47I GGWCC 2 cut(s) 1171, 1252
Eco57I CTGAAG 2 cut(s) 522, 1437
Eco81I CCTNAGG 1 cut(s) 1167
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 1171
EcoRV GATATC 1 cut(s) 347
EcoT14I CCWWGG 3 cut(s) 35, 899, 1256
ErhI CCWWGG 3 cut(s) 35, 899, 1256
FaeI CATG 5 cut(s) 272, 699, 788, 1260, 1306
FalI AAGNNNNNCTT 4 cut(s) 737, 769, 858, 890
FaqI GGGAC 2 cut(s) 1159, 1238
FatI CATG 5 cut(s) 268, 695, 784, 1256, 1302
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 5 cut(s) 110, 210, 329, 839, 1449
FokI GGATG 6 cut(s) 147, 746, 813, 979, 1171, 1211
Fsp4HI GCNGC 5 cut(s) 110, 210, 329, 839, 1449
FspBI CTAG 4 cut(s) 206, 300, 464, 900
GlaI GCGC 2 cut(s) 202, 1391
GluI GCNGC 5 cut(s) 110, 210, 329, 839, 1449
HaeII RGCGCY 1 cut(s) 1393
HaeIII GGCC 4 cut(s) 44, 971, 1332, 1386
HapII CCGG 4 cut(s) 48, 494, 1325, 1383
HhaI GCGC 2 cut(s) 203, 1392
Hin1II CATG 5 cut(s) 272, 699, 788, 1260, 1306
Hin6I GCGC 2 cut(s) 201, 1390
HinP1I GCGC 2 cut(s) 201, 1390
HincII GTYRAC 2 cut(s) 154, 1060
HindII GTYRAC 2 cut(s) 154, 1060
HinfI GANTC 6 cut(s) 379, 623, 649, 681, 717, 1411
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 4 cut(s) 48, 494, 1325, 1383
Hpy166II GTNNAC 5 cut(s) 154, 563, 751, 1027, 1060
Hpy188I TCNGA 7 cut(s) 526, 686, 733, 917, 1178, 1204, 1416
Hpy188III TCNNGA 7 cut(s) 117, 269, 494, 721, 1108, 1123, 1340
Hpy8I GTNNAC 5 cut(s) 154, 563, 751, 1027, 1060
Hpy99I CGWCG 1 cut(s) 960
HpyAV CCTTC 4 cut(s) 500, 1129, 1197, 1322
HpyCH4III ACNGT 4 cut(s) 770, 1075, 1246, 1367
HpyCH4IV ACGT 2 cut(s) 871, 958
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 209
HpyF3I CTNAG 3 cut(s) 409, 1167, 1429
HpySE526I ACGT 2 cut(s) 871, 958
Hsp92II CATG 5 cut(s) 272, 699, 788, 1260, 1306
HspAI GCGC 2 cut(s) 201, 1390
Kpn2I TCCGGA 1 cut(s) 493
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 3 cut(s) 265, 490, 728
LmnI GCTCC 3 cut(s) 242, 496, 851
Lsp1109I GCAGC 5 cut(s) 96, 221, 340, 825, 1460
LweI GCATC 4 cut(s) 81, 125, 835, 1012
MaeI CTAG 4 cut(s) 206, 300, 464, 900
MaeII ACGT 2 cut(s) 871, 958
MaeIII GTNAC 3 cut(s) 319, 883, 1376
MalI GATC 3 cut(s) 267, 492, 730
MboI GATC 3 cut(s) 265, 490, 728
MboII GAAGA 6 cut(s) 275, 515, 545, 1373, 1415, 1435
MfeI CAATTG 1 cut(s) 384
MluCI AATT 4 cut(s) 334, 384, 520, 994
MmeI TCCRAC 2 cut(s) 1063, 1267
MroI TCCGGA 1 cut(s) 493
MseI TTAA 7 cut(s) 9, 153, 237, 744, 756, 951, 1037
MslI CAYNNNNRTG 2 cut(s) 831, 1153
MspI CCGG 4 cut(s) 48, 494, 1325, 1383
MspR9I CCNGG 1 cut(s) 1383
MunI CAATTG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 144, 209
NciI CCSGG 1 cut(s) 1383
NcoI CCATGG 1 cut(s) 1256
NdeI CATATG 1 cut(s) 367
NdeII GATC 3 cut(s) 265, 490, 728
NlaIII CATG 5 cut(s) 272, 699, 788, 1260, 1306
NlaIV GGNNCC 4 cut(s) 244, 498, 1164, 1254
NmuCI GTSAC 3 cut(s) 319, 883, 1376
NspV TTCGAA 1 cut(s) 189
PagI TCATGA 1 cut(s) 268
PceI AGGCCT 1 cut(s) 1332
PfeI GAWTC 6 cut(s) 379, 623, 649, 681, 717, 1411
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 5 cut(s) 111, 211, 330, 840, 1450
PpuMI RGGWCCY 1 cut(s) 1171
PshAI GACNNNNGTC 1 cut(s) 1092
Psp5II RGGWCCY 1 cut(s) 1171
PspN4I GGNNCC 4 cut(s) 244, 498, 1164, 1254
PspPI GGNCC 3 cut(s) 970, 1171, 1252
PspPPI RGGWCCY 1 cut(s) 1171
PsrI GAACNNNNNNTAC 2 cut(s) 553, 585
PstNI CAGNNNCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
RseI CAYNNNNRTG 2 cut(s) 831, 1153
SaqAI TTAA 7 cut(s) 9, 153, 237, 744, 756, 951, 1037
SatI GCNGC 5 cut(s) 110, 210, 329, 839, 1449
Sau3AI GATC 3 cut(s) 265, 490, 728
Sau96I GGNCC 3 cut(s) 970, 1171, 1252
ScaI AGTACT 1 cut(s) 893
ScrFI CCNGG 1 cut(s) 1383
SfaNI GCATC 4 cut(s) 81, 125, 835, 1012
SfcI CTRYAG 2 cut(s) 790, 1020
SfuI TTCGAA 1 cut(s) 189
SinI GGWCC 2 cut(s) 1171, 1252
SmiMI CAYNNNNRTG 2 cut(s) 831, 1153
Sse9I AATT 4 cut(s) 334, 384, 520, 994
SseBI AGGCCT 1 cut(s) 1332
SsiI CCGC 1 cut(s) 291
SspI AATATT 1 cut(s) 474
SspMI CTAG 4 cut(s) 206, 300, 464, 900
StuI AGGCCT 1 cut(s) 1332
StyD4I CCNGG 1 cut(s) 1381
StyI CCWWGG 3 cut(s) 35, 899, 1256
TaaI ACNGT 4 cut(s) 770, 1075, 1246, 1367
TaiI ACGT 2 cut(s) 874, 961
TaqI TCGA 5 cut(s) 116, 189, 343, 1100, 1359
TaqII GACCGA 1 cut(s) 1240
TasI AATT 4 cut(s) 334, 384, 520, 994
TatI WGTACW 1 cut(s) 891
TfiI GAWTC 6 cut(s) 379, 623, 649, 681, 717, 1411
Tru1I TTAA 7 cut(s) 9, 153, 237, 744, 756, 951, 1037
Tru9I TTAA 7 cut(s) 9, 153, 237, 744, 756, 951, 1037
TscAI CASTG 3 cut(s) 64, 178, 775
TseFI GTSAC 3 cut(s) 319, 883, 1376
TseI GCWGC 5 cut(s) 109, 209, 328, 838, 1448
Tsp45I GTSAC 3 cut(s) 319, 883, 1376
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 3 cut(s) 64, 178, 775
VpaK11BI GGWCC 2 cut(s) 1171, 1252
XagI CCTNNNNNAGG 1 cut(s) 504
XmaJI CCTAGG 1 cut(s) 899
XspI CTAG 4 cut(s) 206, 300, 464, 900
ZrmI AGTACT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.