Rorug07G0052300

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008078
Physical Location & Seq
Reverse (-)
3734535 .. 3736813
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0052300.1

Sequence Viewer

Length: 618 bp
ATGGCGTTCTTGAGTAAATTCGGCAATATACTTAAACAGACCGCAACCAGGCAGATCAGATCTGAATTGGCTCCTTCCAAGCCGTCGATTTTTCAGGCCATAAGATGCATGTCAACTGGGCCAAGTTCTAAACTATTTATTGGAGGTGTGTCATTTCAGAGTGATGAGCAAAGTTTGAGGGAAGAATTTGCCAAATATGGTGAAGTTGTTGATGTAAGAATCATCACAGACCGTGACACTGGTAGATCGAGAGGCTTTGGATTCGTTACTTACACATCTAGCGAGGAGGCATCAAGTGCCATCCAGGCCTTGGATGGTCAGGAGCTTCATGGCCGCCGCGTCAGGGTGAATTATGCTACTGACAGGCCTCGGACTAACTTTGGAGGAGGTGGCAGCTATGGTTCTGGGAACTCTGGTGGTGGATACGGTGGAAGTGGAGTTGGCTACAACAGCGGTGGTACTTTTGATAATGCTAGCACTGGTGGTTTTGGTGGTACTGACAACAGCTTTTCTGCTGGTGGAGCTCAATTTGGAGAGGACAAAAGTGGCTTCGATGAGCCATTAGAAGGAAACAATAGGGATGATGATGACACTGCAGACTTCGAAAAAAGGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

205

Amino Acids

21.7

Weight (kDa)

5.01

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 45 - 115 5.5e-22 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 1 cut(s) 339
AciI CCGC 4 cut(s) 42, 334, 337, 453
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 2 cut(s) 17, 185
AfaI GTAC 2 cut(s) 460, 496
AfiI CCNNNNNNNGG 4 cut(s) 48, 310, 343, 566
AjnI CCWGG 2 cut(s) 47, 303
AluBI AGCT 4 cut(s) 325, 396, 507, 524
AluI AGCT 4 cut(s) 325, 396, 507, 524
Alw21I GWGCWC 1 cut(s) 526
AoxI GGCC 6 cut(s) 96, 119, 306, 331, 365, 612
ApeKI GCWGC 1 cut(s) 393
ApoI RAATTY 2 cut(s) 17, 185
AspS9I GGNCC 2 cut(s) 119, 612
AsuHPI GGTGA 2 cut(s) 212, 358
AsuII TTCGAA 1 cut(s) 603
AsuNHI GCTAGC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 308, 308
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 2 cut(s) 49, 305
BciVI GTATCC 1 cut(s) 416
BfaI CTAG 2 cut(s) 279, 474
BfmI CTRYAG 1 cut(s) 594
BfuI GTATCC 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 305
BglII AGATCT 1 cut(s) 59
BisI GCNGC 3 cut(s) 334, 337, 394
BlsI GCNGC 3 cut(s) 335, 338, 395
Bme1390I CCNGG 2 cut(s) 49, 305
BmgT120I GGNCC 2 cut(s) 119, 612
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 49, 305
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 2 cut(s) 95, 299
BmtI GCTAGC 1 cut(s) 477
BmuI ACTGGG 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 603
BpuEI CTTGAG 1 cut(s) 31
BsaJI CCNNGG 2 cut(s) 309, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 310, 343, 566
Bse1I ACTGG 3 cut(s) 121, 244, 484
BseBI CCWGG 2 cut(s) 49, 305
BseDI CCNNGG 2 cut(s) 309, 368
BseGI GGATG 3 cut(s) 300, 319, 586
BseLI CCNNNNNNNGG 4 cut(s) 48, 310, 343, 566
BseNI ACTGG 3 cut(s) 121, 244, 484
BseRI GAGGAG 2 cut(s) 299, 399
BseXI GCAGC 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 6 cut(s) 98, 121, 308, 333, 367, 614
BsiHKAI GWGCWC 1 cut(s) 526
BslI CCNNNNNNNGG 4 cut(s) 48, 310, 343, 566
BsnI GGCC 6 cut(s) 98, 121, 308, 333, 367, 614
Bsp119I TTCGAA 1 cut(s) 603
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 3 cut(s) 54, 59, 245
BspACI CCGC 4 cut(s) 42, 334, 337, 453
BspANI GGCC 6 cut(s) 98, 121, 308, 333, 367, 614
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 598
BspOI GCTAGC 1 cut(s) 477
BspT104I TTCGAA 1 cut(s) 603
BsrI ACTGG 3 cut(s) 121, 244, 484
BssECI CCNNGG 2 cut(s) 309, 368
BssMI GATC 3 cut(s) 54, 59, 245
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 49, 305
Bst4CI ACNGT 2 cut(s) 233, 428
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 475
BstF5I GGATG 3 cut(s) 300, 319, 586
BstFNI CGCG 1 cut(s) 339
BstKTI GATC 3 cut(s) 57, 62, 248
BstMBI GATC 3 cut(s) 54, 59, 245
BstMWI GCNNNNNNNGC 4 cut(s) 296, 305, 450, 521
BstNI CCWGG 2 cut(s) 49, 305
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 47, 303
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 59
BstYI RGATCY 1 cut(s) 59
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 6 cut(s) 98, 121, 308, 333, 367, 614
BtsCI GGATG 3 cut(s) 300, 319, 586
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 3 cut(s) 237, 477, 591
Cac8I GCNNGC 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 119, 612
CseI GACGC 1 cut(s) 328
Csp6I GTAC 2 cut(s) 459, 495
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 2 cut(s) 109, 329
CviQI GTAC 2 cut(s) 459, 495
DpnI GATC 3 cut(s) 56, 61, 247
DpnII GATC 3 cut(s) 54, 59, 245
EaeI YGGCCR 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 309
Eco147I AGGCCT 2 cut(s) 308, 367
Eco24I GRGCYC 1 cut(s) 526
Eco53kI GAGCTC 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 612
EcoRII CCWGG 2 cut(s) 47, 303
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 110
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 2 cut(s) 112, 332
FaiI YATR 7 cut(s) 29, 101, 110, 198, 330, 354, 399
FatI CATG 2 cut(s) 108, 328
Fnu4HI GCNGC 3 cut(s) 334, 337, 394
FokI GGATG 3 cut(s) 287, 326, 593
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 3 cut(s) 334, 337, 394
FspBI CTAG 2 cut(s) 279, 474
GluI GCNGC 3 cut(s) 334, 337, 394
HaeIII GGCC 6 cut(s) 98, 121, 308, 333, 367, 614
HgaI GACGC 1 cut(s) 328
Hin1II CATG 2 cut(s) 112, 332
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 219, 261
HphI GGTGA 2 cut(s) 212, 358
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 4 cut(s) 59, 64, 159, 372
Hpy188III TCNNGA 3 cut(s) 10, 249, 320
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 2 cut(s) 84, 560
HpyCH4III ACNGT 2 cut(s) 233, 428
HpyCH4V TGCA 2 cut(s) 108, 596
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 305, 450, 521
Hsp92II CATG 2 cut(s) 112, 332
Kzo9I GATC 3 cut(s) 54, 59, 245
LmnI GCTCC 3 cut(s) 76, 322, 521
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 2 cut(s) 95, 299
MaeI CTAG 2 cut(s) 279, 474
MaeIII GTNAC 2 cut(s) 233, 265
MalI GATC 3 cut(s) 56, 61, 247
MboI GATC 3 cut(s) 54, 59, 245
MboII GAAGA 1 cut(s) 194
MflI RGATCY 1 cut(s) 59
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 5 cut(s) 17, 65, 185, 349, 527
MnlI CCTC 9 cut(s) 137, 171, 245, 277, 280, 377, 378, 380, 529
Mph1103I ATGCAT 1 cut(s) 110
MseI TTAA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 453
MspR9I CCNGG 2 cut(s) 49, 305
MvaI CCWGG 2 cut(s) 49, 305
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 4 cut(s) 296, 305, 450, 521
NdeII GATC 3 cut(s) 54, 59, 245
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 2 cut(s) 112, 332
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 110
NspI RCATGY 1 cut(s) 112
NspV TTCGAA 1 cut(s) 603
PceI AGGCCT 2 cut(s) 308, 367
PfeI GAWTC 2 cut(s) 219, 261
PflMI CCANNNNNTGG 1 cut(s) 310
PkrI GCNGC 3 cut(s) 335, 338, 395
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 2 cut(s) 47, 303
PspGI CCWGG 2 cut(s) 47, 303
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 119, 612
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 59
RsaI GTAC 2 cut(s) 460, 496
RsaNI GTAC 2 cut(s) 459, 495
SacI GAGCTC 1 cut(s) 526
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 334, 337, 394
Sau3AI GATC 3 cut(s) 54, 59, 245
Sau96I GGNCC 2 cut(s) 119, 612
ScrFI CCNGG 2 cut(s) 49, 305
SduI GDGCHC 1 cut(s) 526
SetI ASST 6 cut(s) 148, 327, 391, 398, 509, 526
SfaNI GCATC 2 cut(s) 95, 299
SfcI CTRYAG 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 5 cut(s) 17, 65, 185, 349, 527
SseBI AGGCCT 2 cut(s) 308, 367
SsiI CCGC 4 cut(s) 42, 334, 337, 453
SspMI CTAG 2 cut(s) 279, 474
SstI GAGCTC 1 cut(s) 526
StuI AGGCCT 2 cut(s) 308, 367
StyD4I CCNGG 2 cut(s) 47, 303
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 233, 428
TaqI TCGA 4 cut(s) 86, 248, 552, 603
TasI AATT 5 cut(s) 17, 65, 185, 349, 527
TauI GCSGC 2 cut(s) 336, 339
TfiI GAWTC 2 cut(s) 219, 261
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 3 cut(s) 244, 484, 598
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 393
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 3 cut(s) 244, 484, 598
Van91I CCANNNNNTGG 1 cut(s) 310
XapI RAATTY 2 cut(s) 17, 185
XceI RCATGY 1 cut(s) 112
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 311
XspI CTAG 2 cut(s) 279, 474
Zsp2I ATGCAT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.