Rroxscaffold_3G00257180

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008078
Physical Location & Seq
Forward (+)
51576128 .. 51580661
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257180.1

Sequence Viewer

Length: 1500 bp
ATGGCAGTTAAACTGGAACAAGAGCCAATTACACCCTGGCCGGCCACCGGTCGCACCATTGCCACGAATTGCATCATCTGTTTCCAATCTGGTGCTTATGCCTCCAAACTGCTATCACGAAATGATGACAATGGATGCAATCCTAGTGAGGTTAACAAGGCAAGGGAGAACTCTACAGCAGACACTGGAAATGCTAACTTAGAGAAAGCTGGTGCACTAGCGTCTAAGTTCTTGGCAAAGTTGAACATTAAAGGAGCCAACAAAGGTGGGAAAGAAGATTATGAAACATCTCAAAAAAATGACAGAAATGATGCTAATCTATTTAGCAATGAAGTGACAAAGCAGCCTAATTCCCCTAGAAATTCAAACGCATCAAGTGTATATGGGCATAAGAATCCGATTGATTTAGATGAGAGTTCTGTCTTAGCCTCTTCAAGGGAGTGCAATGAGTATATTGGGTTGCATAGACCAAATCATCTATTGCCAAAATTACACTATGCAAATGATCAGGGGCCTGAAGAATGCAACTTGAATGCTTCAGAGGTAGAAGATGCAATGCAAAAACAACCAGATGGTCAACCATTACCTAGTTCAGCAGAGAGCACTCGCAGCCCCAATAGTTCATCAAGCAAAGGGGTTAATAATTCTACTTCCATAGGAGGTTGTGAGATTCGTTGGGAGGACTTACATTTGGGAGAGGAAATTGGACAAGGTTCCTGTGCTATTGTATATCGTGGAATCTGGAATGGATCGGATGTTGCCATTAAGGTTTACTTTAAAAATGAATACAGTGAGGGGGTTTTGCATGACTACAGAAAGGAGATTGATATAATGAAAAGATTGCGGCATCCTAATGTGCTGCTATTTATGGGAGCAGTATATTCACAAGAACGTCTGGCTATTGTCACAGAGTACCTACCTAGGGGAAGTCTTTTCAGACAACTTCACAAAAACAATCAAGCATTGGATATTAAACGACGTATGAGGATGGCCCTTGATGTTGCGAGAGGTATGAATTACTTGCATCGTAGAAATCCACCTATAGTTCACAGAGACTTAAAATCGTCTAACTTGCTGGTTGATAAGAACTGGACTGTCAAGGTTGGAGACTTTGGTCTGTCGAAGTTAAAGAACGCAACCTTCTTGACTGCAAAATCTGGGAGAGGAACACCTCAGTGGATGGCACCTGAAGTCCTCCGAAATGAACCTTCAAATGAGAAGTCTGATGTATTTAGCTTTGGTGTCATCCTTTGGGAACTAATGACTGTATCGGTCCCATGGAATAACCTCAATCCCTTGCAGGTTGTTGGAGTTGTAGGCTTCATGGATAGAAGATTAGACATACCAGAAGGCCTCAATCCCGAAGTAGTATCCATTATTGAAGACTGTTGGCGTAGTGACCCAGGACAGCGTCCATCGTTTGAAGGTGTGATTCAGAGAATGAAGAACTTGGTTATCAAAGTTGAAGCAGCTTCAGTAAGAAGAAGTTCAGACCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

499

Amino Acids

55.56

Weight (kDa)

8.02

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 229 - 480 2.8e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 230 - 479 5.1e-54 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1291
AccB1I GGYRCC 1 cut(s) 1183
AciI CCGC 1 cut(s) 844
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 2 cut(s) 38, 42
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 4 cut(s) 522, 537, 1209, 1458
AfaI GTAC 1 cut(s) 914
AfiI CCNNNNNNNGG 3 cut(s) 47, 435, 922
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 8 cut(s) 244, 366, 435, 532, 1212, 1382, 1424, 1466
AjnI CCWGG 2 cut(s) 35, 1402
AleI CACNNNNGTG 1 cut(s) 1174
AluBI AGCT 3 cut(s) 209, 1236, 1472
AluI AGCT 3 cut(s) 209, 1236, 1472
Alw21I GWGCWC 2 cut(s) 217, 605
Alw26I GTCTC 2 cut(s) 1047, 1101
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 1 cut(s) 757
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 5 cut(s) 38, 42, 512, 990, 1351
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 4 cut(s) 343, 609, 859, 1469
ApoI RAATTY 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 1486
AspA2I CCTAGG 1 cut(s) 920
AspS9I GGNCC 3 cut(s) 512, 991, 1273
AvaII GGWCC 1 cut(s) 1273
AvrII CCTAGG 1 cut(s) 920
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 1183
BbsI GAAGAC 1 cut(s) 1389
Bbv12I GWGCWC 2 cut(s) 217, 605
BbvI GCAGC 4 cut(s) 355, 621, 846, 1481
BccI CCATC 4 cut(s) 566, 982, 1174, 1423
BciT130I CCWGG 2 cut(s) 37, 1404
BciVI GTATCC 1 cut(s) 1381
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 1047, 1101
BfaI CTAG 5 cut(s) 144, 218, 357, 588, 921
BfmI CTRYAG 3 cut(s) 174, 811, 1041
BfuAI ACCTGC 1 cut(s) 1291
BfuI GTATCC 1 cut(s) 1381
BisI GCNGC 5 cut(s) 344, 610, 845, 860, 1470
BlnI CCTAGG 1 cut(s) 920
BlsI GCNGC 5 cut(s) 345, 611, 846, 861, 1471
Bme1390I CCNGG 2 cut(s) 37, 1404
Bme18I GGWCC 1 cut(s) 1273
BmgT120I GGNCC 3 cut(s) 512, 991, 1273
BmiI GGNNCC 5 cut(s) 256, 513, 715, 1185, 1275
BmrFI CCNGG 2 cut(s) 37, 1404
BmsI GCATC 7 cut(s) 81, 125, 301, 380, 541, 856, 1033
BoxI GACNNNNGTC 1 cut(s) 1113
BpiI GAAGAC 1 cut(s) 1389
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 35, 920, 1277, 1402
BsaWI WCCGGW 1 cut(s) 47
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 435, 922
Bse118I RCCGGY 2 cut(s) 40, 47
Bse1I ACTGG 3 cut(s) 18, 190, 1094
Bse3DI GCAATG 4 cut(s) 57, 334, 451, 561
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 2 cut(s) 37, 1404
BseDI CCNNGG 4 cut(s) 35, 920, 1277, 1402
BseGI GGATG 6 cut(s) 140, 760, 847, 993, 1185, 1245
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 3 cut(s) 47, 435, 922
BseMI GCAATG 4 cut(s) 57, 334, 451, 561
BseMII CTCAG 1 cut(s) 1187
BseNI ACTGG 3 cut(s) 18, 190, 1094
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 4 cut(s) 355, 621, 846, 1481
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 5 cut(s) 40, 44, 514, 992, 1353
BshNI GGYRCC 1 cut(s) 1183
BshTI ACCGGT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 217, 605
BsiSI CCGG 2 cut(s) 41, 48
BslFI GGGAC 1 cut(s) 1259
BslI CCNNNNNNNGG 3 cut(s) 47, 435, 922
BsmAI GTCTC 2 cut(s) 1047, 1101
BsmFI GGGAC 1 cut(s) 1259
BsmI GAATGC 2 cut(s) 527, 538
BsnI GGCC 5 cut(s) 40, 44, 514, 992, 1353
Bsp1286I GDGCHC 2 cut(s) 217, 605
Bsp143I GATC 2 cut(s) 505, 749
Bsp19I CCATGG 1 cut(s) 1277
BspACI CCGC 1 cut(s) 844
BspANI GGCC 5 cut(s) 40, 44, 514, 992, 1353
BspCNI CTCAG 1 cut(s) 1186
BspLI GGNNCC 5 cut(s) 256, 513, 715, 1185, 1275
BspMI ACCTGC 1 cut(s) 1291
BspPI GGATC 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 1183
BsrDI GCAATG 4 cut(s) 57, 334, 451, 561
BsrFI RCCGGY 2 cut(s) 40, 47
BsrI ACTGG 3 cut(s) 18, 190, 1094
BssAI RCCGGY 2 cut(s) 40, 47
BssECI CCNNGG 4 cut(s) 35, 920, 1277, 1402
BssMI GATC 2 cut(s) 505, 749
BssT1I CCWWGG 2 cut(s) 920, 1277
Bst2UI CCWGG 2 cut(s) 37, 1404
Bst4CI ACNGT 4 cut(s) 791, 1096, 1267, 1388
Bst6I CTCTTC 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 4 cut(s) 199, 225, 424, 1173
BstDSI CCRYGG 1 cut(s) 1277
BstENI CCTNNNNNAGG 1 cut(s) 433
BstF5I GGATG 6 cut(s) 140, 760, 847, 993, 1185, 1245
BstKTI GATC 2 cut(s) 508, 752
BstMAI GTCTC 2 cut(s) 1047, 1101
BstMBI GATC 2 cut(s) 505, 749
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 609
BstNI CCWGG 2 cut(s) 37, 1404
BstPAI GACNNNNGTC 1 cut(s) 1113
BstSCI CCNGG 2 cut(s) 35, 1402
BstSFI CTRYAG 3 cut(s) 174, 811, 1041
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 4 cut(s) 355, 621, 846, 1481
BstV2I GAAGAC 1 cut(s) 1389
BsuI GTATCC 1 cut(s) 1381
BsuRI GGCC 5 cut(s) 40, 44, 514, 992, 1353
BtgI CCRYGG 1 cut(s) 1277
BtsCI GGATG 6 cut(s) 140, 760, 847, 993, 1185, 1245
BtsIMutI CAGTG 3 cut(s) 183, 796, 1181
BveI ACCTGC 1 cut(s) 1291
Cac8I GCNNGC 1 cut(s) 42
CaiI CAGNNNCTG 1 cut(s) 185
Cfr10I RCCGGY 2 cut(s) 40, 47
Cfr13I GGNCC 3 cut(s) 512, 991, 1273
CseI GACGC 2 cut(s) 210, 1400
Csp6I GTAC 1 cut(s) 913
CspAI ACCGGT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 3 cut(s) 806, 1278, 1324
CviQI GTAC 1 cut(s) 913
DdeI CTNAG 4 cut(s) 199, 225, 424, 1173
DpnI GATC 2 cut(s) 507, 751
DpnII GATC 2 cut(s) 505, 749
DraI TTTAAA 1 cut(s) 778
EaeI YGGCCR 2 cut(s) 38, 42
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 920, 1277
Eco147I AGGCCT 1 cut(s) 1353
Eco47I GGWCC 1 cut(s) 1273
Eco57I CTGAAG 4 cut(s) 522, 537, 1209, 1458
EcoNI CCTNNNNNAGG 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 35, 1402
EcoT14I CCWWGG 2 cut(s) 920, 1277
ErhI CCWWGG 2 cut(s) 920, 1277
FaeI CATG 3 cut(s) 809, 1281, 1327
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 1 cut(s) 1259
FatI CATG 3 cut(s) 805, 1277, 1323
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 5 cut(s) 344, 610, 845, 860, 1470
FokI GGATG 6 cut(s) 147, 767, 834, 1000, 1192, 1232
FseI GGCCGGCC 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 344, 610, 845, 860, 1470
FspBI CTAG 5 cut(s) 144, 218, 357, 588, 921
GluI GCNGC 5 cut(s) 344, 610, 845, 860, 1470
HaeIII GGCC 5 cut(s) 40, 44, 514, 992, 1353
HapII CCGG 2 cut(s) 41, 48
HgaI GACGC 2 cut(s) 210, 1400
Hin1II CATG 3 cut(s) 809, 1281, 1327
HincII GTYRAC 2 cut(s) 154, 578
HindII GTYRAC 2 cut(s) 154, 578
HinfI GANTC 4 cut(s) 394, 670, 738, 1432
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 2 cut(s) 41, 48
Hpy166II GTNNAC 5 cut(s) 154, 215, 578, 772, 1048
Hpy188I TCNGA 8 cut(s) 399, 541, 754, 938, 1199, 1225, 1437, 1492
Hpy188III TCNNGA 4 cut(s) 117, 742, 1144, 1361
Hpy8I GTNNAC 5 cut(s) 154, 215, 578, 772, 1048
Hpy99I CGWCG 1 cut(s) 981
HpyAV CCTTC 4 cut(s) 1150, 1218, 1343, 1418
HpyCH4III ACNGT 4 cut(s) 791, 1096, 1267, 1388
HpyCH4IV ACGT 2 cut(s) 892, 979
HpyF10VI GCNNNNNNNGC 1 cut(s) 609
HpyF3I CTNAG 4 cut(s) 199, 225, 424, 1173
HpySE526I ACGT 2 cut(s) 892, 979
Hsp92II CATG 3 cut(s) 809, 1281, 1327
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 505
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 505, 749
LmnI GCTCC 2 cut(s) 254, 872
Lsp1109I GCAGC 4 cut(s) 355, 621, 846, 1481
LweI GCATC 7 cut(s) 81, 125, 301, 380, 541, 856, 1033
MaeI CTAG 5 cut(s) 144, 218, 357, 588, 921
MaeII ACGT 2 cut(s) 892, 979
MaeIII GTNAC 3 cut(s) 334, 904, 1397
MalI GATC 2 cut(s) 507, 751
MboI GATC 2 cut(s) 505, 749
MboII GAAGA 8 cut(s) 287, 423, 530, 560, 1344, 1394, 1456, 1494
MhlI GDGCHC 2 cut(s) 217, 605
MluCI AATT 8 cut(s) 27, 67, 349, 361, 488, 643, 702, 1015
MmeI TCCRAC 2 cut(s) 1084, 1288
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 1486
MslI CAYNNNNRTG 2 cut(s) 852, 1174
MspI CCGG 2 cut(s) 41, 48
MspR9I CCNGG 2 cut(s) 37, 1404
Mva1269I GAATGC 2 cut(s) 527, 538
MvaI CCWGG 2 cut(s) 37, 1404
MwoI GCNNNNNNNGC 1 cut(s) 609
NaeI GCCGGC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 1277
NdeII GATC 2 cut(s) 505, 749
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 809, 1281, 1327
NlaIV GGNNCC 5 cut(s) 256, 513, 715, 1185, 1275
NmuCI GTSAC 3 cut(s) 334, 904, 1397
OliI CACNNNNGTG 1 cut(s) 1174
PceI AGGCCT 1 cut(s) 1353
PctI GAATGC 2 cut(s) 527, 538
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 1486
PfeI GAWTC 4 cut(s) 394, 670, 738, 1432
PflFI GACNNNGTC 1 cut(s) 1410
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 5 cut(s) 345, 611, 846, 861, 1471
PshAI GACNNNNGTC 1 cut(s) 1113
Psp6I CCWGG 2 cut(s) 35, 1402
PspGI CCWGG 2 cut(s) 35, 1402
PspN4I GGNNCC 5 cut(s) 256, 513, 715, 1185, 1275
PspPI GGNCC 3 cut(s) 512, 991, 1273
PstNI CAGNNNCTG 1 cut(s) 185
PsyI GACNNNGTC 1 cut(s) 1410
RigI GGCCGGCC 1 cut(s) 44
RsaI GTAC 1 cut(s) 914
RsaNI GTAC 1 cut(s) 913
RseI CAYNNNNRTG 2 cut(s) 852, 1174
SatI GCNGC 5 cut(s) 344, 610, 845, 860, 1470
Sau3AI GATC 2 cut(s) 505, 749
Sau96I GGNCC 3 cut(s) 512, 991, 1273
ScrFI CCNGG 2 cut(s) 37, 1404
SduI GDGCHC 2 cut(s) 217, 605
SfaNI GCATC 7 cut(s) 81, 125, 301, 380, 541, 856, 1033
SfcI CTRYAG 3 cut(s) 174, 811, 1041
SinI GGWCC 1 cut(s) 1273
SmiMI CAYNNNNRTG 2 cut(s) 852, 1174
Sse9I AATT 8 cut(s) 27, 67, 349, 361, 488, 643, 702, 1015
SseBI AGGCCT 1 cut(s) 1353
SsiI CCGC 1 cut(s) 844
SspMI CTAG 5 cut(s) 144, 218, 357, 588, 921
StuI AGGCCT 1 cut(s) 1353
StyD4I CCNGG 2 cut(s) 35, 1402
StyI CCWWGG 2 cut(s) 920, 1277
TaaI ACNGT 4 cut(s) 791, 1096, 1267, 1388
TaiI ACGT 2 cut(s) 895, 982
TaqI TCGA 1 cut(s) 1121
TaqII GACCGA 1 cut(s) 1261
TasI AATT 8 cut(s) 27, 67, 349, 361, 488, 643, 702, 1015
TauI GCSGC 1 cut(s) 847
TfiI GAWTC 4 cut(s) 394, 670, 738, 1432
TscAI CASTG 3 cut(s) 190, 796, 1181
TseFI GTSAC 3 cut(s) 334, 904, 1397
TseI GCWGC 4 cut(s) 343, 609, 859, 1469
Tsp45I GTSAC 3 cut(s) 334, 904, 1397
TspDTI ATGAA 9 cut(s) 297, 345, 612, 798, 848, 1028, 1218, 1312, 1457
TspRI CASTG 3 cut(s) 190, 796, 1181
Tth111I GACNNNGTC 1 cut(s) 1410
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 1273
XagI CCTNNNNNAGG 1 cut(s) 433
XapI RAATTY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XmaJI CCTAGG 1 cut(s) 920
XmnI GAANNNNTTC 1 cut(s) 1486
XspI CTAG 5 cut(s) 144, 218, 357, 588, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.